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dc.contributor.authorMohamed, Mohamed F.
dc.contributor.authorAbdelkhalek, Ahmed
dc.contributor.authorSeleem, Mohamed N.
dc.date.accessioned2020-09-21T16:15:31Z
dc.date.available2020-09-21T16:15:31Z
dc.date.issued2016-07-11
dc.identifierARTN 29707 (Article number)
dc.identifier.issn2045-2322
dc.identifier.othersrep29707 (PII)
dc.identifier.urihttp://hdl.handle.net/10919/100037
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) infections present a serious challenge because of the emergence of resistance to numerous conventional antibiotics. Due to their unique mode of action, antimicrobial peptides are novel alternatives to traditional antibiotics for tackling the issue of bacterial multidrug resistance. Herein, we investigated the antibacterial activity of two short novel peptides (WR12, a 12 residue peptide composed exclusively of arginine and tryptophan, and D-IK8, an eight residue β-sheet peptide) against multidrug resistant staphylococci. In vitro, both peptides exhibited good antibacterial activity against MRSA, vancomycin-resistant S. aureus, linezolid-resistant S. aureus, and methicillin-resistant S. epidermidis. WR12 and D-IK8 were able to eradicate persisters, MRSA in stationary growth phase, and showed significant clearance of intracellular MRSA in comparison to both vancomycin and linezolid. In vivo, topical WR12 and D-IK8 significantly reduced both the bacterial load and the levels of the pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in MRSA-infected skin lesions. Moreover, both peptides disrupted established in vitro biofilms of S. aureus and S. epidermidis significantly more so than traditional antimicrobials tested. Taken together, these results support the potential of WR12 and D-IK8 to be used as a topical antimicrobial agent for the treatment of staphylococcal skin infections.en
dc.format.extent14 page(s)
dc.format.mediumElectronic
dc.languageEnglish
dc.publisherNature Publishing Group
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSOFT-TISSUE INFECTIONS
dc.subjectPERSISTER CELLS
dc.subjectANTIBIOTICS
dc.subjectMECHANISMS
dc.subjectDISEASE
dc.subjectSKIN
dc.subjectEPIDERMIDIS
dc.subjectVANCOMYCIN
dc.subjectPEXIGANAN
dc.subjectPENETRATION
dc.subject.meshKeratinocytes
dc.subject.meshHumans
dc.subject.meshStaphylococcus epidermidis
dc.subject.meshStaphylococcal Skin Infections
dc.subject.meshAntimicrobial Cationic Peptides
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshMethicillin-Resistant Staphylococcus aureus
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshAntimicrobial Cationic Peptides
dc.subject.meshHumans
dc.subject.meshKeratinocytes
dc.subject.meshMethicillin-Resistant Staphylococcus aureus
dc.subject.meshStaphylococcal Skin Infections
dc.subject.meshStaphylococcus epidermidis
dc.titleEvaluation of short synthetic antimicrobial peptides for treatment of drug-resistant and intracellular Staphylococcus aureusen
dc.typeArticle - Refereeden
dc.date.updated2020-09-21T16:15:27Z
dc.description.versionPublished (Publication status)
dc.title.serialScientific Reportsen
dc.identifier.doihttps://doi.org/10.1038/srep29707
dc.type.otherArticle
dc.type.otherJournal
dc.identifier.volume6
dc.identifier.issue1
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458 (orcid)]
dc.identifier.pmid27405275 (pubmed)
dcterms.dateAccepted2016-06-21
dc.identifier.eissn2045-2322
pubs.organisational-group/Virginia Tech/Veterinary Medicine
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiology
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Faculty
pubs.organisational-group/Virginia Tech


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Creative Commons Attribution 4.0 International
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