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dc.contributor.authorMohammad, Haroon
dc.contributor.authorCushman, Mark
dc.contributor.authorSeleem, Mohamed N.
dc.date.accessioned2020-09-21T16:17:58Z
dc.date.available2020-09-21T16:17:58Z
dc.date.issued2015-11-04
dc.identifierARTN e0142321 (Article number)
dc.identifier.issn1932-6203
dc.identifier.otherPONE-D-15-22389 (PII)
dc.identifier.urihttp://hdl.handle.net/10919/100045
dc.description.abstractThe emergence of community-associated methicillin-resistant Staphylococcus aureus (MRSA), including strains resistant to current antibiotics, has contributed to an increase in the number of skin infections reported in humans in recent years. New therapeutic options are needed to counter this public health challenge. The aim of the present study was to examine the potential of thiazole compounds synthesized by our research group to be used topically to treat MRSA skin and wound infections. The broth microdilution method confirmed that the lead thiazole compound and four analogues are capable of inhibiting MRSA growth at concentrations as low as 1.3 μg/mL. Additionally, three compounds exhibited a synergistic relationship when combined with the topical antibiotic mupirocin against MRSA in vitro via the checkerboard assay. Thus the thiazole compounds have potential to be used alone or in combination with mupirocin against MRSA. When tested against human keratinocytes, four derivatives of the lead compound demonstrated an improved toxicity profile (were found to be non-toxic up to a concentration of 20 μg/mL). Utilizing a murine skin infection model, we confirmed that the lead compound and three analogues exhibited potent antimicrobial activity in vivo, with similar capability as the antibiotic mupirocin, as they reduced the burden of MRSA present in skin wounds by more than 90%. Taken altogether, the present study provides important evidence that these thiazole compounds warrant further investigation for development as novel topical antimicrobials to treat MRSA skin infections.en
dc.format.extent13 page(s)
dc.format.mediumElectronic-eCollection
dc.languageEnglish
dc.publisherPLoS
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMUPIROCIN RESISTANCE
dc.subjectVANCOMYCIN
dc.subjectANTIBIOTICS
dc.subjectCOMBINATION
dc.subjectEPIDEMIOLOGY
dc.subjectCLINDAMYCIN
dc.subjectAMOXICILLIN
dc.subjectCLAVULANATE
dc.subjectEMERGENCE
dc.subjectGENES
dc.subject.meshKeratinocytes
dc.subject.meshAnimals
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshStaphylococcal Infections
dc.subject.meshStaphylococcal Skin Infections
dc.subject.meshThiazoles
dc.subject.meshMupirocin
dc.subject.meshDrug Therapy, Combination
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshAdministration, Topical
dc.subject.meshMethicillin-Resistant Staphylococcus aureus
dc.subject.meshAdministration, Topical
dc.subject.meshAnimals
dc.subject.meshDrug Therapy, Combination
dc.subject.meshHumans
dc.subject.meshKeratinocytes
dc.subject.meshMethicillin-Resistant Staphylococcus aureus
dc.subject.meshMice
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshMupirocin
dc.subject.meshStaphylococcal Infections
dc.subject.meshStaphylococcal Skin Infections
dc.subject.meshThiazoles
dc.titleAntibacterial Evaluation of Synthetic Thiazole Compounds In Vitro and In Vivo in a Methicillin-Resistant Staphylococcus aureus (MRSA) Skin Infection Mouse Modelen
dc.typeArticle - Refereed
dc.date.updated2020-09-21T16:17:56Z
dc.description.versionPublished (Publication status)
dc.title.serialPLOS ONE
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0142321
dc.type.otherArticle
dc.type.otherJournal
dc.identifier.volume10
dc.identifier.issue11
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458 (orcid)]
dc.identifier.pmid26536129 (pubmed)
dcterms.dateAccepted2015-10-19
dc.identifier.eissn1932-6203
pubs.organisational-group/Virginia Tech/Veterinary Medicine
pubs.organisational-group/Virginia Tech/Faculty of Health Sciences
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiology
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Faculty
pubs.organisational-group/Virginia Tech


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Creative Commons Attribution 4.0 International
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