Abstract
            
            
               
                  Background
               
               We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.
            
            
               
                  Methods
               
               Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.
            
            
               
                  Results
               
               By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.
            
            
               
                  Conclusion
               
               This is the first study demonstrated the occurrence of SHV-12 in Nigeria.