Gene Ontology annotation of the rice blast fungus, Magnaporthe oryzae

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dc.contributor.author Meng, Shaowu
dc.contributor.author Brown, Douglas E
dc.contributor.author Ebbole, Daniel J
dc.contributor.author Torto-Alalibo, Trudy
dc.contributor.author Oh, Yeon Y
dc.contributor.author Deng, Jixin
dc.contributor.author Mitchell, Thomas K
dc.contributor.author Dean, Ralph A
dc.date.accessioned 2012-08-24T11:47:31Z
dc.date.available 2012-08-24T11:47:31Z
dc.date.issued 2009-02-19
dc.identifier.citation BMC Microbiology. 2009 Feb 19;9(Suppl 1):S8
dc.identifier.uri http://hdl.handle.net/10919/18873
dc.description.abstract Abstract Background Magnaporthe oryzae, the causal agent of blast disease of rice, is the most destructive disease of rice worldwide. The genome of this fungal pathogen has been sequenced and an automated annotation has recently been updated to Version 6 http://www.broad.mit.edu/annotation/genome/magnaporthe_grisea/MultiDownloads.html. However, a comprehensive manual curation remains to be performed. Gene Ontology (GO) annotation is a valuable means of assigning functional information using standardized vocabulary. We report an overview of the GO annotation for Version 5 of M. oryzae genome assembly. Methods A similarity-based (i.e., computational) GO annotation with manual review was conducted, which was then integrated with a literature-based GO annotation with computational assistance. For similarity-based GO annotation a stringent reciprocal best hits method was used to identify similarity between predicted proteins of M. oryzae and GO proteins from multiple organisms with published associations to GO terms. Significant alignment pairs were manually reviewed. Functional assignments were further cross-validated with manually reviewed data, conserved domains, or data determined by wet lab experiments. Additionally, biological appropriateness of the functional assignments was manually checked. Results In total, 6,286 proteins received GO term assignment via the homology-based annotation, including 2,870 hypothetical proteins. Literature-based experimental evidence, such as microarray, MPSS, T-DNA insertion mutation, or gene knockout mutation, resulted in 2,810 proteins being annotated with GO terms. Of these, 1,673 proteins were annotated with new terms developed for Plant-Associated Microbe Gene Ontology (PAMGO). In addition, 67 experiment-determined secreted proteins were annotated with PAMGO terms. Integration of the two data sets resulted in 7,412 proteins (57%) being annotated with 1,957 distinct and specific GO terms. Unannotated proteins were assigned to the 3 root terms. The Version 5 GO annotation is publically queryable via the GO site http://amigo.geneontology.org/cgi-bin/amigo/go.cgi. Additionally, the genome of M. oryzae is constantly being refined and updated as new information is incorporated. For the latest GO annotation of Version 6 genome, please visit our website http://scotland.fgl.ncsu.edu/smeng/GoAnnotationMagnaporthegrisea.html. The preliminary GO annotation of Version 6 genome is placed at a local MySql database that is publically queryable via a user-friendly interface Adhoc Query System. Conclusion Our analysis provides comprehensive and robust GO annotations of the M. oryzae genome assemblies that will be solid foundations for further functional interrogation of M. oryzae.
dc.title Gene Ontology annotation of the rice blast fungus, Magnaporthe oryzae
dc.type Journal Article
dc.date.updated 2012-08-24T11:47:31Z
dc.description.version Peer Reviewed
dc.language.rfc3066 en
dc.rights.holder Shaowu Meng et al.; licensee BioMed Central Ltd.
dc.identifier.doi http://dx.doi.org/10.1186/1471-2180-9-S1-S8

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