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dc.contributor.authorMeng, Shaowuen_US
dc.contributor.authorBrown, Douglas Een_US
dc.contributor.authorEbbole, Daniel Jen_US
dc.contributor.authorTorto-Alalibo, Trudyen_US
dc.contributor.authorOh, Yeon Yen_US
dc.contributor.authorDeng, Jixinen_US
dc.contributor.authorMitchell, Thomas K.en_US
dc.contributor.authorDean, Ralph Aen_US
dc.identifier.citationBMC Microbiology. 2009 Feb 19;9(Suppl 1):S8en_US
dc.description.abstractAbstract Background Magnaporthe oryzae, the causal agent of blast disease of rice, is the most destructive disease of rice worldwide. The genome of this fungal pathogen has been sequenced and an automated annotation has recently been updated to Version 6 However, a comprehensive manual curation remains to be performed. Gene Ontology (GO) annotation is a valuable means of assigning functional information using standardized vocabulary. We report an overview of the GO annotation for Version 5 of M. oryzae genome assembly. Methods A similarity-based (i.e., computational) GO annotation with manual review was conducted, which was then integrated with a literature-based GO annotation with computational assistance. For similarity-based GO annotation a stringent reciprocal best hits method was used to identify similarity between predicted proteins of M. oryzae and GO proteins from multiple organisms with published associations to GO terms. Significant alignment pairs were manually reviewed. Functional assignments were further cross-validated with manually reviewed data, conserved domains, or data determined by wet lab experiments. Additionally, biological appropriateness of the functional assignments was manually checked. Results In total, 6,286 proteins received GO term assignment via the homology-based annotation, including 2,870 hypothetical proteins. Literature-based experimental evidence, such as microarray, MPSS, T-DNA insertion mutation, or gene knockout mutation, resulted in 2,810 proteins being annotated with GO terms. Of these, 1,673 proteins were annotated with new terms developed for Plant-Associated Microbe Gene Ontology (PAMGO). In addition, 67 experiment-determined secreted proteins were annotated with PAMGO terms. Integration of the two data sets resulted in 7,412 proteins (57%) being annotated with 1,957 distinct and specific GO terms. Unannotated proteins were assigned to the 3 root terms. The Version 5 GO annotation is publically queryable via the GO site Additionally, the genome of M. oryzae is constantly being refined and updated as new information is incorporated. For the latest GO annotation of Version 6 genome, please visit our website The preliminary GO annotation of Version 6 genome is placed at a local MySql database that is publically queryable via a user-friendly interface Adhoc Query System. Conclusion Our analysis provides comprehensive and robust GO annotations of the M. oryzae genome assemblies that will be solid foundations for further functional interrogation of M. oryzae.en_US
dc.rightsAttribution 4.0 United States*
dc.titleGene Ontology annotation of the rice blast fungus, Magnaporthe oryzaeen_US
dc.typeJournal articleen_US
dc.description.versionPeer Revieweden_US
dc.rights.holderShaowu Meng et al.; licensee BioMed Central Ltd.en_US

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Attribution 4.0 United States
Except where otherwise noted, this item's license is described as Attribution 4.0 United States