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Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages

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dc.contributor.author Mian, MA Rouf
dc.contributor.author Zhang, Yan
dc.contributor.author Wang, Zeng-Yu
dc.contributor.author Zhang, Ji-Yi
dc.contributor.author Cheng, Xiaofei
dc.contributor.author Chen, Lei
dc.contributor.author Chekhovskiy, Konstantin
dc.contributor.author Dai, Xinbin
dc.contributor.author Mao, Chunhong
dc.contributor.author Cheung, Foo
dc.contributor.author Zhao, Xuechun
dc.contributor.author He, Ji
dc.contributor.author Scott, Angela D
dc.contributor.author Town, Christopher D
dc.contributor.author May, Gregory D
dc.date.accessioned 2012-08-24T12:21:39Z
dc.date.available 2012-08-24T12:21:39Z
dc.date.issued 2008-03-04
dc.identifier.citation BMC Plant Biology. 2008 Mar 04;8(1):27
dc.identifier.uri http://dx.doi.org/10.1186/1471-2229-8-27
dc.identifier.uri http://hdl.handle.net/10919/18980
dc.description.abstract Abstract Background Tall fescue (Festuca arundinacea Schreb) is a major cool season forage and turf grass species grown in the temperate regions of the world. In this paper we report the generation of a tall fescue expressed sequence tag (EST) database developed from nine cDNA libraries representing tissues from different plant organs, developmental stages, and abiotic stress factors. The results of inter-library and library-specific in silico expression analyses of these ESTs are also reported. Results A total of 41,516 ESTs were generated from nine cDNA libraries of tall fescue representing tissues from different plant organs, developmental stages, and abiotic stress conditions. The Festuca Gene Index (FaGI) has been established. To date, this represents the first publicly available tall fescue EST database. In silico gene expression studies using these ESTs were performed to understand stress responses in tall fescue. A large number of ESTs of known stress response gene were identified from stressed tissue libraries. These ESTs represent gene homologues of heat-shock and oxidative stress proteins, and various transcription factor protein families. Highly expressed ESTs representing genes of unknown functions were also identified in the stressed tissue libraries. Conclusion FaGI provides a useful resource for genomics studies of tall fescue and other closely related forage and turf grass species. Comparative genomic analyses between tall fescue and other grass species, including ryegrasses (Lolium sp.), meadow fescue (F. pratensis) and tetraploid fescue (F. arundinacea var glaucescens) will benefit from this database. These ESTs are an excellent resource for the development of simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) PCR-based molecular markers.
dc.title Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages
dc.type Journal Article
dc.date.updated 2012-08-24T12:21:39Z
dc.description.version Peer Reviewed
dc.language.rfc3066 en
dc.rights.holder MA Rouf Mian et al.; licensee BioMed Central Ltd.


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  • BioMed Central [327]
    BioMed Central publications by Virginia Tech authors.

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