Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia

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dc.contributor.author Bowerman, Peter A
dc.contributor.author Ramirez, Melissa V
dc.contributor.author Price, Michelle B
dc.contributor.author Helm, Richard F
dc.contributor.author Winkel, Brenda SJ
dc.date.accessioned 2012-12-18T20:08:22Z
dc.date.available 2012-12-18T20:08:22Z
dc.date.issued 2012-09-04
dc.identifier.citation BMC Research Notes. 2012 Sep 04;5(1):485
dc.identifier.uri http://hdl.handle.net/10919/19095
dc.description.abstract Abstract Background The flavonoid pathway is a long-standing and important tool for plant genetics, biochemistry, and molecular biology. Numerous flavonoid mutants have been identified in Arabidopsis over the past several decades in a variety of ecotypes. Here we present an analysis of Arabidopsis lines of ecotype Columbia carrying T-DNA insertions in genes encoding enzymes of the central flavonoid pathway. We also provide a comprehensive summary of various mutant alleles for these structural genes that have been described in the literature to date in a wide variety of ecotypes. Findings The confirmed knockout lines present easily-scorable phenotypes due to altered pigmentation of the seed coat (or testa). Knockouts for seven alleles for six flavonoid biosynthetic genes were confirmed by PCR and characterized by UPLC for altered flavonol content. Conclusion Seven mutant lines for six genes of the central flavonoid pathway were characterized in ecotype, Columbia. These lines represent a useful resource for integrating biochemical and physiological studies with genomic, transcriptomic, and proteomic data, much of which has been, and continues to be, generated in the Columbia background.
dc.title Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia
dc.type Journal Article
dc.date.updated 2012-12-18T20:08:22Z
dc.description.version Peer Reviewed
dc.language.rfc3066 en
dc.rights.holder Peter A Bowerman et al.; licensee BioMed Central Ltd.
dc.identifier.doi http://dx.doi.org/10.1186/1756-0500-5-485

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    BioMed Central publications by Virginia Tech authors.

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