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dc.contributor.authorBabahosseini, Hesamen
dc.date.accessioned2017-10-18T04:18:16Zen
dc.date.available2017-10-18T04:18:16Zen
dc.date.issued2015-09-25en
dc.identifier.otheretd-10022015-090213en
dc.identifier.urihttp://hdl.handle.net/10919/79689en
dc.description.abstractCancer progression and physiological changes within the cells are accompanied by alterations in the biophysical properties. Therefore, the cell biophysical properties can serve as promising markers for cancer detection and physiological activities. To aid in the investigation of the biophysical markers of cells, a microfluidic chip has been developed which consists of a constriction channel and embedded microelectrodes. Single-cell impedance magnitudes at four frequencies and entry and travel times are measured simultaneously during their transit through the constriction channel. This microchip provides a high-throughput, label-free, automated assay to define biophysical signatures of malignant cells and monitor the therapeutic efficacy of drugs. Here, we monitored the dynamic cellular biophysical markers in response to sphingosine kinase inhibitors (SphKIs), and compared the effectiveness of drug delivery using Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with SphKIs versus conventional delivery. Cells treated with SphKIs showed significantly higher impedance magnitudes at all four frequencies. The bioelectrical parameters extracted using a model also revealed that the highly aggressive breast cells treated with SphKIs shifted electrically towards that of a less malignant phenotype; SphKI-treated cells exhibited an increase in cell-channel interface resistance and a significant decrease in specific membrane capacitance. Furthermore, SphKI-treated cells became slightly more deformable as measured by a decrease in their channel entry and travel times. We observed no significant difference in the bioelectrical changes produced by SphKI delivered conventionally or with NPs. However, NPs-packaged delivery of SphKI decreased the cell deformability. In summary, the results showed that while the bioelectrical properties of the cells were dominantly affected by SphKIs, the biomechanical properties were mainly changed by the NPs.en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectMicroElectroMechanical Systems (MEMS)en
dc.subjectMicrofluidicsen
dc.subjectBiosensoren
dc.subjectNanoparticlesen
dc.subjectDrug Deliveryen
dc.subjectBiomechanicsen
dc.subjectBioelectronicsen
dc.subjectBreast Canceren
dc.subjectMDA-MB-231en
dc.subjectSphingosine Kinase Inhibitorsen
dc.titleNanoparticle-Based Drug Delivery and the Impacts on Cancer Cell Biophysical Markersen
dc.typeThesisen
dc.contributor.departmentBiological Systems Engineeringen
dc.description.degreeMaster of Scienceen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelmastersen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.disciplineBiological Systems Engineeringen
dc.contributor.committeechairZhang, Chenming Mikeen
dc.contributor.committeememberSantos, Websteren
dc.type.dcmitypeTexten
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10022015-090213/en
dc.contributor.committeecochairAgah, Masouden
dc.date.sdate2015-10-02en
dc.date.rdate2015-11-19en
dc.date.adate2015-11-19en


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