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dc.contributor.advisorKornegay, E. T.en_US
dc.contributor.advisorHerbein, J. H. Jr.en_US
dc.contributor.advisorWebb, K. E. Jr.en_US
dc.contributor.authorBowers, Sharon H.en_US
dc.date.accessioned2011-08-06T14:36:31Z
dc.date.available2011-08-06T14:36:31Z
dc.date.issued1997-08-19en_US
dc.identifier.otheretd-81097-11925en_US
dc.identifier.urihttp://hdl.handle.net/10919/9589
dc.description.abstractIn order to characterize peptide transport in the ovine small intestine, [14C]-glycyl-sarcosine uptake by tissue collected from five sheep was studied through the use of brush border membrane vesicles (BBMV). Preliminary experiments determined that incubation in hyaluronidase is not necessary in order to separate mucosal tissue from the basement membrane and that the stop solution used in the uptake study needed to be buffered. Uptake was examined in proximal (denoted jejunal) and distal (denoted ileal) halves of the intestine at four times (15, 30, 45, and 60 s) and at three extravesicular pH levels (6.4, 7.0, and 7.5). An intravesicular pH of 7.5 was used throughout the study. The two tissue sites differed (P < .02), with BBMV from jejunal tissue showing greater uptake than ileal. Uptake plateaued after 45 s, resulting in a quadratic (P < .005) effect of time. The effect of changes in extravesicular pH was also quadratic (P < .04), with uptake being greatest at pH 6.4, lowest at pH 7.0 and intermediate between the two at pH 7.5. Peptide uptake by sheep jejunal and ileal BBMV was demonstrated, but there was no clear evidence for increased uptake with decreasing extravesicular pH.en_US
dc.format.mediumETDen_US
dc.publisherVirginia Techen_US
dc.relation.haspartthesis.pdfen_US
dc.rightsThe authors of the theses and dissertations are the copyright owners. Virginia Tech's Digital Library and Archives has their permission to store and provide access to these works.en_US
dc.source.urihttp://scholar.lib.vt.edu/theses/available/etd-81097-11925en_US
dc.subjectSheepen_US
dc.subjectJejunumen_US
dc.subjectIleumen_US
dc.subjectPeptideen_US
dc.subjectAbsorptionen_US
dc.subjectTransporten_US
dc.titleCharacterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesiclesen_US
dc.typeThesisen_US
dc.contributor.departmentAnimal and Poultry Sciencesen_US
dc.description.degreeMaster of Scienceen_US


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