Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles

We are planning to upgrade VTechWorks to DSpace 5.2 on August 7th, 2015. The site may be down for a few hours on that day, and you may see some differences in the site. Write vtechworks@vt.edu with any concerns or comments.

Show simple item record

dc.contributor.advisor Kornegay, E. T. en_US
dc.contributor.advisor Herbein, J. H. Jr. en_US
dc.contributor.advisor Webb, K. E. Jr. en_US
dc.contributor.author Bowers, Sharon H. en_US
dc.date.accessioned 2011-08-06T14:36:31Z
dc.date.available 2011-08-06T14:36:31Z
dc.date.issued 1997-08-19 en_US
dc.identifier.other etd-81097-11925 en_US
dc.identifier.uri http://hdl.handle.net/10919/9589
dc.description.abstract In order to characterize peptide transport in the ovine small intestine, [14C]-glycyl-sarcosine uptake by tissue collected from five sheep was studied through the use of brush border membrane vesicles (BBMV). Preliminary experiments determined that incubation in hyaluronidase is not necessary in order to separate mucosal tissue from the basement membrane and that the stop solution used in the uptake study needed to be buffered. Uptake was examined in proximal (denoted jejunal) and distal (denoted ileal) halves of the intestine at four times (15, 30, 45, and 60 s) and at three extravesicular pH levels (6.4, 7.0, and 7.5). An intravesicular pH of 7.5 was used throughout the study. The two tissue sites differed (P < .02), with BBMV from jejunal tissue showing greater uptake than ileal. Uptake plateaued after 45 s, resulting in a quadratic (P < .005) effect of time. The effect of changes in extravesicular pH was also quadratic (P < .04), with uptake being greatest at pH 6.4, lowest at pH 7.0 and intermediate between the two at pH 7.5. Peptide uptake by sheep jejunal and ileal BBMV was demonstrated, but there was no clear evidence for increased uptake with decreasing extravesicular pH. en_US
dc.format.medium ETD en_US
dc.publisher Virginia Tech en_US
dc.relation.haspart thesis.pdf en_US
dc.rights The authors of the theses and dissertations are the copyright owners. Virginia Tech's Digital Library and Archives has their permission to store and provide access to these works. en_US
dc.source.uri http://scholar.lib.vt.edu/theses/available/etd-81097-11925 en_US
dc.subject Sheep en_US
dc.subject Jejunum en_US
dc.subject Ileum en_US
dc.subject Peptide en_US
dc.subject Absorption en_US
dc.subject Transport en_US
dc.title Characterization of Glycyl-Sarcosine Uptake by Ovine Intestinal Brush Border Membrane Vesicles en_US
dc.type Thesis en_US
dc.contributor.department Animal and Poultry Sciences en_US
dc.description.degree Master of Science en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search VTechWorks

Advanced Search


My Account