Lack of mycothiol and ergothioneine induces different protective mechanisms in Mycobacterium smegmatis.

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dc.contributor.authorSingh, A. R.en
dc.contributor.authorStrankman, A.en
dc.contributor.authorOrkusyan, R.en
dc.contributor.authorPurwantini, E.en
dc.contributor.authorRawat, M.en
dc.contributor.departmentBiochemistryen
dc.coverage.spatialNetherlandsen
dc.date.accessioned2018-01-08T13:32:58Zen
dc.date.available2018-01-08T13:32:58Zen
dc.date.issued2016-12en
dc.description.abstractMycobacterium smegmatis contains the low molecular weight thiols, mycothiol (MSH) and ergothioneine (ESH). Examination of transposon mutants disrupted in mshC and egtA, involved in the biosynthesis of MSH and ESH respectively, demonstrated that both mutants were sensitive to oxidative, alkylating, and metal stress. However, the mshC mutant exhibited significantly more protein carbonylation and lipid peroxidation than wildtype, while the egtA mutant had less protein and lipid damage than wildtype. We further show that Ohr, KatN, and AhpC, involved in protection against oxidative stress, are upregulated in the egtA mutant. In the mshC mutant, an Usp and a putative thiol peroxidase are upregulated. In addition, mutants lacking MSH also contained higher levels of Coenzyme F420 as compared to wildtype and two Coenzyme F420 dependent enzymes were found to be upregulated. These results indicate that lack of MSH and ESH result in induction of different mechanisms for protecting against oxidative stress.en
dc.description.versionPublished versionen
dc.format.extent100 - 106 page(s)en
dc.identifier.doihttps://doi.org/10.1016/j.bbrep.2016.08.006en
dc.identifier.issn2405-5808en
dc.identifier.urihttp://hdl.handle.net/10919/81597en
dc.identifier.volume8en
dc.language.isoenen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/28220152en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCoenzyme F420en
dc.subjectMycothiolen
dc.subjectergothioneineen
dc.subjectmycobacteriaen
dc.subjectoxidative stressen
dc.subjectthiol peroxidaseen
dc.titleLack of mycothiol and ergothioneine induces different protective mechanisms in Mycobacterium smegmatis.en
dc.title.serialBiochemistry and Biophysics Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
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