Immunopathologic effects associated with Sarcocystis neurona-infected interferon-gamma knockout mice

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dc.contributor.authorWitonsky, Sharon G.en
dc.contributor.authorGogal, Robert M.en
dc.contributor.authorDuncan, R. B.en
dc.contributor.authorLindsay, David S.en
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:15Zen
dc.date.available2014-06-20T14:13:15Zen
dc.date.issued2003-10en
dc.description.abstractInterferon-gamma knockout (IFN-gamma KO) mice were infected with Sarcocystis neurona merozoites to characterize the immunopathology associated with infection. By day 14 postinfection (PI), mice developed splenomegaly and lymphadenopathy, characterized by marked lymphoid hyperplasia with increased numbers of germinal centers. Additional histopathologic changes included increased extramedullary hematopoiesis, multifocal mixed inflammatory infiltrates in the liver, perivascular infiltrate of the liver and lung, and interstitial pneumonia. The total number of B-cell splenocytes (P < 0.05) and the percentage of B-cells increased on day 14 PI in the spleen and on day 28 PI in the lymph nodes (P < 0.05). By day 28 PI, the number of B-cell splenocytes decreased significantly. A non-subset-specific decrease in percentages of CD4 lymphocytes throughout all lymphoid organs was observed on day 14 PI. However, total CD4 and CD44/CD4 splenocytes increased significantly by day 28 PI. Early-activation CD8 lymphocytes were reduced in the blood and spleen, whereas memory CD8 lymphocyte percentages and total numbers were significantly increased. On the basis of the results, we propose that S. neurona-infected IFN-gamma KO mice are immunocompromised and unable to clear the infection. Thus, they develop B-cell exhaustion and a delayed, but sustained. increased number of memory CD4 and CD8 lymphocytes due to chronic antigen stimulation.en
dc.description.sponsorshipCooperative State Research, Education, and Extension Service Animal Health and Disease granten
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSharon G. Witonsky, Robert M. Gogal Jr., Robert B. Duncan, and David S. Lindsay (2003). "Immunopathologic effects associated with Sarcocystis neurona-infected interferon-gamma knockout mice," Journal of Parasitology, Vol. 89, No. 5, pp. 932-940. doi: http://dx.doi.org/10.1645/GE-72Ren
dc.identifier.doihttps://doi.org/10.1645/ge-72ren
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49052en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-72Ren
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectequine protozoal myeloencephalitisen
dc.subjectopossum didelphis-virginianaen
dc.subjectcd8(+)en
dc.subjectt-cellsen
dc.subjecttoxoplasma-gondiien
dc.subjecthorsesen
dc.subjectencephalitisen
dc.subjectsporocystsen
dc.subjectantibodiesen
dc.subjectmigrationen
dc.subjectinvasionen
dc.subjectparasitologyen
dc.titleImmunopathologic effects associated with Sarcocystis neurona-infected interferon-gamma knockout miceen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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