Structural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mapping

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dc.contributor.authorMa, Manxiuen
dc.contributor.authorKler, Stanislaven
dc.contributor.authorPan, Yuchin Alberten
dc.date.accessioned2020-05-21T13:02:39Zen
dc.date.available2020-05-21T13:02:39Zen
dc.date.issued2020-01-23en
dc.description.abstractThe unique combination of small size, translucency, and powerful genetic tools makes larval zebrafish a uniquely useful vertebrate system to investigate normal and pathological brain structure and function. While functional connectivity can now be assessed by optical imaging (via fluorescent calcium or voltage reporters) at the whole-brain scale, it remains challenging to systematically determine structural connections and identify connectivity changes during development or disease. To address this, we developed Tracer with Restricted Anterograde Spread (TRAS), a novel vesicular stomatitis virus (VSV)-based neural circuit labeling approach. TRAS makes use of replication-incompetent VSV (VSV Delta G) and a helper virus (lentivirus) to enable anterograde transneuronal spread between efferent axons and their direct postsynaptic targets but restricts further spread to downstream areas. We integrated TRAS with the Z-Brain zebrafish 3D atlas for quantitative connectivity analysis and identified targets of the retinal and habenular efferent projections, in patterns consistent with previous reports. We compared retinofugal connectivity patterns between wild-type and down syndrome cell adhesion molecule-like 1 (dscaml1) mutant zebrafish and revealed differences in topographical distribution. These results demonstrate the utility of TRAS for quantitative structural connectivity analysis that would be valuable for detecting novel efferent targets and mapping connectivity changes underlying neurological or behavioral deficits.en
dc.description.notesThis work was supported by funding from the National Eye Institute of the National Institutes of Health (R01 EY024844 to YP), Center for Innovative Technology (ER14S-001-LS to YP), Augusta University, and Virginia Tech.en
dc.description.sponsorshipNational Eye Institute of the National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Eye Institute (NEI) [R01 EY024844]; Center for Innovative Technology [ER14S-001-LS]; Augusta University; Virginia Techen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fncir.2019.00085en
dc.identifier.eissn1662-5110en
dc.identifier.other85en
dc.identifier.pmid32038180en
dc.identifier.urihttp://hdl.handle.net/10919/98510en
dc.identifier.volume13en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecttranssynapticen
dc.subjectzebrafishen
dc.subjectVSVen
dc.subjectbrain mappingen
dc.subjectviral tracingen
dc.titleStructural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mappingen
dc.title.serialFrontiers in Neural Circuitsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Scholarly Works, Fralin Biomedical Research Institute at VTC
Destination Area: Adaptive Brain and Behavior (ABB)
Scholarly Works, Biomedical Sciences and Pathobiology
Scholarly Works, Virginia Tech Carilion School of Medicine (VTCSOM)