Focused ultrasound extraction (FUSE) for the rapid extraction of DNA from tissue matrices

Simple item page
dc.contributor.authorHolmes, Hal R.en
dc.contributor.authorHaywood, Morganen
dc.contributor.authorHutchison, Rubyen
dc.contributor.authorZhang, Qianen
dc.contributor.authorEdsall, Connoren
dc.contributor.authorHall, Timothy L.en
dc.contributor.authorBaisch, Daviden
dc.contributor.authorHolliday, Jason A.en
dc.contributor.authorVlaisavljevich, Elien
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.contributor.departmentForest Resources and Environmental Conservationen
dc.date.accessioned2020-12-07T14:12:48Zen
dc.date.available2020-12-07T14:12:48Zen
dc.date.issued2020-10-09en
dc.description.abstractRapid DNA extraction is a critical barrier for routine and fieldable genetics tests for applications in conservation, such as illegal trafficking and fraudulent mislabelling. Here, we develop a non-thermal focused ultrasound extraction (FUSE) technique that creates a dense cloud of high-pressure acoustic cavitation bubbles to disintegrate targeted tissues into an acellular debris, resulting in the rapid release of entrapped DNA. In this work, we demonstrate the proof-of-concept of the FUSE technique by obtaining species identifiable sequences and shotgun sequencing reads from DNA extracted from Atlantic salmon Salmo salar tissues. Having mitigated the key risks for this technique, we hypothesize future developments with this technology can be applied to accelerate and simplify DNA extraction from exceedingly difficult samples with complex tissue matrices (i.e. fibrous tissue and timber samples) in both laboratory and field settings.en
dc.description.notesNational Geographic Society; Schmidt Science Fellows; Gordon and Betty Moore Foundation, Grant/Award Number: #8518; Rhodes Trust and Schmidt Futures Foundation; Virginia Tech Department of Biomedical Engineering and Mechanics; Institute for Critical Technology and Applied Science; Center for Engineering Healthen
dc.description.sponsorshipGordon and Betty Moore FoundationGordon and Betty Moore Foundation [8518]; Rhodes Trust; Schmidt Futures Foundation; Virginia Tech Department of Biomedical Engineering and Mechanics; Center for Engineering Health; Institute for Critical Technology and Applied Science; National Geographic SocietyNational Geographic Societyen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/2041-210X.13505en
dc.identifier.eissn2041-2096en
dc.identifier.issn2041-210Xen
dc.identifier.urihttp://hdl.handle.net/10919/101024en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectDNA barcodingen
dc.subjectDNA extractionen
dc.subjectfocused ultrasounden
dc.titleFocused ultrasound extraction (FUSE) for the rapid extraction of DNA from tissue matricesen
dc.title.serialMethods in Ecology and Evolutionen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2041-210X.13505.pdf
Size:
1.26 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Scholarly Works, Biomedical Engineering and Mechanics
Scholarly Works, Forest Resources and Environmental Conservation