Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice

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dc.contributor.authorAvila-Calderon, Eric Danielen
dc.contributor.authorLopez-Merino, Ahidéen
dc.contributor.authorJain, Neetaen
dc.contributor.authorPeralta, Humbertoen
dc.contributor.authorLopez-Villegas, Edgar Oliveren
dc.contributor.authorSriranganathan, Nammalwaren
dc.contributor.authorBoyle, Stephen M.en
dc.contributor.authorWitonsky, Sharon G.en
dc.contributor.authorContreras-Rodriguez, Aracelien
dc.date.accessioned2019-05-20T11:48:23Zen
dc.date.available2019-05-20T11:48:23Zen
dc.date.issued2011-12-29en
dc.date.updated2019-05-20T07:13:40Zen
dc.description.abstractThe outer membrane vesicles (OMVs) from smooth B. melitensis 16 M and a derived rough mutant, VTRM1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. Proteomic analysis showed 29 proteins present in OMVs from B. melitensis 16 M; some of them are well-known Brucella immunogens such as SOD, GroES, Omp31, Omp25, Omp19, bp26, and Omp16. OMVs from a rough VTRM1 induced significantly higher expression of IL-12, TNFa, and IFN? genes in bone marrow dendritic cells than OMVs from smooth strain 16 M. Relative to saline control group, mice immunized intramuscularly with rough and smooth OMVs were protected from challenge with virulent strain B. melitensis 16 M just as well as the group immunized with live strain B. melitensis Rev1 (P < 0.005). Additionally, the levels of serum IgG2a increased in mice vaccinated with OMVs from rough strain VTRM1 consistent with the induction of cell-mediated immunity.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationEric Daniel Avila-Calderón, Ahidé Lopez-Merino, Neeta Jain, Humberto Peralta, Edgar Oliver López-Villegas, Nammalwar Sriranganathan, Stephen M. Boyle, Sharon Witonsky, Araceli Contreras-Rodríguez, "Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice", Journal of Immunology Research, vol. 2012, Article ID 352493, 13 pages, 2012. https://doi.org/10.1155/2012/352493en
dc.identifier.doihttps://doi.org/10.1155/2012/352493en
dc.identifier.urihttp://hdl.handle.net/10919/89572en
dc.language.isoenen
dc.publisherHindawi Publishing Corpen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderCopyright © 2012 Eric Daniel Avila-Calderon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleCharacterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Miceen
dc.title.serialJournal of Immunology Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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