Analysis of the antigenic determinants of the OspC protein of the Lyme disease spirochetes: Evidence that the C10 motif is not immunodominant or required to elicit bactericidal antibody responses

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dc.contributor.authorIzac, Jerilyn R.en
dc.contributor.authorCamire, Andrew C.en
dc.contributor.authorEarnhart, Christopher G.en
dc.contributor.authorEmbers, Monica E.en
dc.contributor.authorFunk, Rebecca A.en
dc.contributor.authorBreitschwerdt, Edward B.en
dc.contributor.authorMarconi, Richard T.en
dc.date.accessioned2019-08-29T14:23:43Zen
dc.date.available2019-08-29T14:23:43Zen
dc.date.issued2019-04-17en
dc.description.abstractAs Ixodes ticks spread to new regions, the incidence of Lyme disease (LD) in companion animals and humans will increase. Preventive strategies for LD in canines center on vaccination and tick control (acaricides). Both subunit and bacterin based LD veterinary vaccines are available. Outer surface protein C (OspC), a potent immunogen and dominant early antigen, has been demonstrated to elicit protective antibody (Ab) responses. However, a single OspC protein elicits a relatively narrow range of protection. There are conflicting reports as to whether the immunodominant epitopes of OspC reside within variable or conserved domains. A detailed understanding of the antigenic determinants of OspC is essential for understanding immune responses to this essential virulence factor and vaccinogen. Here, we investigate the contribution of the conserved C-terminal C10 motif in OspC triggered Ab responses. Using a panel of diverse recombinant full length OspC proteins and their corresponding C10 deletion variants (OspC Delta C10), we demonstrate that the C10 motif does not significantly contribute to immunization or infection induced Ab responses in rabbits, rats, canines, horses and non-human primates. Furthermore, the C10 motif is not required to trigger potent bactericidal Ab responses. This study provides insight into the antigenic structure of OspC. The results enhance our understanding of immune responses that develop during infection or upon vaccination and have implications for interpretation of LD diagnostic assays that employ OspC. (C) 2019 The Authors. Published by Elsevier Ltd.en
dc.description.notesSteven and Alexandra Cohen Foundation, NY, USA; National Institutes of Health, NIAID, Bethesda, MD, R56AI127801.en
dc.description.sponsorshipNational Institutes of Health, NIAID, Bethesda, MD [R56AI127801]; Steven and Alexandra Cohen Foundation, NY, USAen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.vaccine.2019.02.007en
dc.identifier.eissn1873-2518en
dc.identifier.issn0264-410Xen
dc.identifier.issue17en
dc.identifier.pmid30922701en
dc.identifier.urihttp://hdl.handle.net/10919/93300en
dc.identifier.volume37en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBorreliellaen
dc.subjectLyme diseaseen
dc.subjectOspCen
dc.subjectLyme disease vaccineen
dc.subjectBorreliaen
dc.titleAnalysis of the antigenic determinants of the OspC protein of the Lyme disease spirochetes: Evidence that the C10 motif is not immunodominant or required to elicit bactericidal antibody responsesen
dc.title.serialVaccineen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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