Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effects

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dc.contributor.authorDeng, Zhaoxien
dc.contributor.authorDai, Tianen
dc.contributor.authorZhang, Wenmingen
dc.contributor.authorZhu, Junlien
dc.contributor.authorLuo, Xin M.en
dc.contributor.authorFu, Dongyanen
dc.contributor.authorLiu, Jianxinen
dc.contributor.authorWang, Haifengen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-01-04T12:53:34Zen
dc.date.available2021-01-04T12:53:34Zen
dc.date.issued2020-12-21en
dc.date.updated2020-12-24T15:29:33Zen
dc.description.abstractThe ability to adhere to the intestinal mucus layer is an important property of probiotic bacteria. <i>Lactobacillus reuteri</i> strains ZJ615 and ZJ617 show low and high adhesion, respectively, to intestinal epithelial cells. In this study, we quantified bacterial cell wall-associated glyceraldehyde-3-phosphate dehydrogenases (cw-GAPDH) and bacterial cell membrane permeability in both strains using immunoblotting and flow cytometry, respectively. Highly adhesive <i>L. reuteri</i> ZJ617 possessed significantly more cw-GAPDH, higher cell membrane permeability, and significantly higher adhesive ability toward mucin compared with low-adhesive <i>L. reuteri</i> ZJ615. In vitro adhesion studies and analysis of interaction kinetics using the Octet, the system revealed significantly decreased interaction between <i>L. reuteri</i> and mucin when mucin was oxidized when bacterial surface proteins were removed when bacteria were heat-inactivated at 80 &deg;C for 30 min, and when the interaction was blocked with an anti-GAPDH antibody. SWISS-MODEL analysis suggested intensive interactions between mucin glycans (GalNAc&alpha;1-O-Ser, GalNAc&alpha;Ser, and Gal&beta;3GalNAc) and GAPDH. Furthermore, in vivo studies revealed significantly higher numbers of bacteria adhering to the jejunum, ileum, and colon of piglets orally inoculated with <i>L. reuteri</i> ZJ617 compared with those inoculated with <i>L. reuteri</i> ZJ615; this led to a significantly decreased rate of diarrhea in piglets inoculated with <i>L. reuteri</i> ZJ617. In conclusion, there are strong correlations among the abundance of cw-GAPDH in <i>L. reuteri</i>, the ability of the bacterium to adhere to the host, and the health benefits of this probiotic.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDeng, Z.; Dai, T.; Zhang, W.; Zhu, J.; Luo, X.M.; Fu, D.; Liu, J.; Wang, H. Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effects. Int. J. Mol. Sci. 2020, 21, 9756.en
dc.identifier.doihttps://doi.org/10.3390/ijms21249756en
dc.identifier.urihttp://hdl.handle.net/10919/101728en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLactobacillus reuterien
dc.subjectGAPDHen
dc.subjectadhesionen
dc.subjectmucinen
dc.subjectintestineen
dc.titleGlyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effectsen
dc.title.serialInternational Journal of Molecular Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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