dc.contributor.author	Lazar, Iuliana M.	en
dc.contributor.author	Kabulski, Jarod L.	en
dc.contributor.department	Biological Sciences	en
dc.date.accessed	2015-04-17	en
dc.date.accessioned	2015-04-20T21:04:11Z	en
dc.date.available	2015-04-20T21:04:11Z	en
dc.date.issued	2013-03-20	en
dc.description.abstract	A microfluidic device that enables on-chip matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) detection for liquid chromatography (LC) separations is described. The device comprises an array of functional elements to carry out LC separations, integrates a novel microchip-MS interface to facilitate the orthogonal transposition of the microfluidic LC channel into an array of reservoirs, and enables sensitive MALDI-MS detection directly from the chip. Essentially, the device provides a snapshot MALDI-MS map of the content of the separation channel present on the chip. The detection of proteins with biomarker potential from MCF10A breast epithelial cell extracts, and detection limits in the low fmol range, are demonstrated. In addition, the design of the novel LC-MALDI-MS chip entices the promotion of a new concept for performing sample separations within the limited time-frame that accompanies the dead-volume of a separation channel.	en
dc.description.notes	Supplementary information is included in separate files	en
dc.description.sponsorship	National Institutes of Health. National Cancer Institute - Award Number R21CA126669-01A1	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/vnd.ms-excel	en
dc.format.mimetype	image/jpeg	en
dc.identifier.citation	Lazar, I. M., & Kabulski, J. L. (2013). Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection. Lab on a Chip, 13(11), 2055-2065. doi: 10.1039/C3LC50190F	en
dc.identifier.doi	https://doi.org/10.1039/C3LC50190F	en
dc.identifier.issn	1473-0197	en
dc.identifier.uri	http://hdl.handle.net/10919/51708	en
dc.identifier.url	http://pubs.rsc.org/en/content/articlelanding/2013/lc/c3lc50190f	en
dc.language.iso	en_US	en
dc.publisher	The Royal Society of Chemistry	en
dc.rights	In Copyright	en
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/	en
dc.subject	Mass spectrometry	en
dc.subject	Liquid chromatography	en
dc.subject	Matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)	en
dc.title	Microfluidic LC Device with Orthogonal Sample Extraction for On-Chip MALDI-MS Detection	en
dc.title.serial	Lab on a Chip	en
dc.type	Article - Refereed	en
dc.type	Dataset	en
dc.type	Image	en
dc.type.dcmitype	Text	en
dc.type.dcmitype	Dataset	en
dc.type.dcmitype	Image	en

Microfluidic LC Device with Orthogonal Sample Extraction for On-Chip MALDI-MS Detection

Files

Original bundle

Collections