Analysis of the in planta transcriptome expressed by the corn pathogen Pantoea stewartii subsp. stewartii via RNA-Seq
| dc.contributor.author | Packard, Holly | en |
| dc.contributor.author | Burke, Alison K. | en |
| dc.contributor.author | Jensen, Roderick V. | en |
| dc.contributor.author | Stevens, Ann M. | en |
| dc.contributor.department | Biological Sciences | en |
| dc.date.accessioned | 2018-01-12T12:58:31Z | en |
| dc.date.available | 2018-01-12T12:58:31Z | en |
| dc.date.issued | 2017-04-27 | en |
| dc.description.abstract | Pantoea stewartii subsp. stewartii is a bacterial phytopathogen that causes Stewart’s wilt disease in corn. It uses quorum sensing to regulate expression of some genes involved in virulence in a cell density-dependent manner as the bacterial population grows from small numbers at the initial infection site in the leaf apoplast to high cell numbers in the xylem where it forms a biofilm. There are also other genes important for pathogenesis not under quorum-sensing control such as a Type III secretion system. The purpose of this study was to compare gene expression during an in planta infection versus either a pre-inoculum in vitro liquid culture or an in vitro agar plate culture to identify genes specifically expressed in planta that may also be important for colonization and/or virulence. RNA was purified from each sample type to determine the transcriptome via RNA-Seq using Illumina sequencing of cDNA. Fold gene expression changes in the in planta data set in comparison to the two in vitro grown samples were determined and a list of the most differentially expressed genes was generated to elucidate genes important for plant association. Quantitative reverse transcription PCR (qRT-PCR) was used to validate expression patterns for a select subset of genes. Analysis of the transcriptome data via gene ontology revealed that bacterial transporters and systems important for oxidation reduction processes appear to play a critical role for P. stewartii as it colonizes and causes wilt disease in corn plants. | en |
| dc.identifier.doi | https://doi.org/10.7717/peerj.3237 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/81748 | en |
| dc.language.iso | en_US | en |
| dc.publisher | PeerJ | en |
| dc.rights | Creative Commons Attribution 3.0 United States | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | en |
| dc.subject | Transcriptome analysis | en |
| dc.subject | Pantoea stewartii | en |
| dc.subject | Phytopathogen | en |
| dc.subject | Corn | en |
| dc.subject | RNA-Seq | en |
| dc.title | Analysis of the in planta transcriptome expressed by the corn pathogen Pantoea stewartii subsp. stewartii via RNA-Seq | en |
| dc.title.serial | PeerJ | en |
| dc.type | Article - Refereed | en |