The olfactory basis of orchid pollination by mosquitoes

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dc.contributor.authorLahondère, Chloéen
dc.contributor.authorVinauger, Clémenten
dc.contributor.authorOkubo, Ryo P.en
dc.contributor.authorWolff, Gabriella H.en
dc.contributor.authorChan, Jeremy K.en
dc.contributor.authorAkbari, Omar S.en
dc.contributor.authorRiffell, Jeffrey A.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2020-02-17T15:40:02Zen
dc.date.available2020-02-17T15:40:02Zen
dc.date.issued2020-01-07en
dc.date.updated2020-02-17T15:39:53Zen
dc.description.abstractMosquitoes are important vectors of disease and require sources of carbohydrates for reproduction and survival. Unlike host-related behaviors of mosquitoes, comparatively less is understood about the mechanisms involved in nectar-feeding decisions, or how this sensory information is processed in the mosquito brain. Here we show that Aedes spp. mosquitoes, including Aedes aegypti, are effective pollinators of the Platanthera obtusata orchid, and demonstrate this mutualism is mediated by the orchid’s scent and the balance of excitation and inhibition in the mosquito’s antennal lobe (AL). The P. obtusata orchid emits an attractive, nonanal-rich scent, whereas related Platanthera species—not visited by mosquitoes—emit scents dominated by lilac aldehyde. Calcium imaging experiments in the mosquito AL revealed that nonanal and lilac aldehyde each respectively activate the LC2 and AM2 glomerulus, and remarkably, the AM2 glomerulus is also sensitive to N,N-diethylmeta-toluamide (DEET), a mosquito repellent. Lateral inhibition between these 2 glomeruli reflects the level of attraction to the orchid scents. Whereas the enriched nonanal scent of P. obtusata activates the LC2 and suppresses AM2, the high level of lilac aldehyde in the other orchid scents inverts this pattern of glomerular activity, and behavioral attraction is lost. These results demonstrate the ecological importance of mosquitoes beyond operating as disease vectors and open the door toward understanding the neural basis of mosquito nectar-seeking behaviors.en
dc.description.sponsorshipSupport for this project was provided by National Institutes of Health under grants RO1-DC013693 (J.A.R.) and R21-AI137947 (J.A.R.), Air Force Office of Scientific Research under grants FA9550-14-1-0398 (J.A.R.) and FA9550-16-1-0167 (J.A.R.), an Endowed Professorship for Excellence in Biology (J.A.R.), and the University of Washington Innovation Award (J.A.R.).en
dc.description.versionPublished (Publication status)en
dc.format.extentPages 708-716en
dc.format.extent9 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1073/pnas.1910589117en
dc.identifier.eissn1091-6490en
dc.identifier.issn0027-8424en
dc.identifier.issue1en
dc.identifier.orcidVinauger Tella, Clement [0000-0002-3704-5427]en
dc.identifier.orcidLahondere, Chloe [0000-0003-1949-7865]en
dc.identifier.other1910589117 (PII)en
dc.identifier.pmid31871198en
dc.identifier.urihttp://hdl.handle.net/10919/96852en
dc.identifier.volume117en
dc.language.isoenen
dc.publisherNational Academy of Sciencesen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000506001200095&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectSILENE-OTITESen
dc.subjectODORANT RECEPTORSen
dc.subjectINHIBITIONen
dc.subjectATTRACTANTSen
dc.subjectPREFERENCESen
dc.subjectMALARIAen
dc.subjectDEETen
dc.subjectAedes aegyptien
dc.subjectPlatantheraen
dc.subjectmosquitoesen
dc.subjectnectaren
dc.subjectolfactionen
dc.titleThe olfactory basis of orchid pollination by mosquitoesen
dc.title.serialProceedings of the National Academy of Sciences of the United States of Americaen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Techen

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