Additional file 7
BLAST results for Unigenes in Additional file 6
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDG55HJ01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_10358
Length=471
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 263882018
Number of extensions: 6073336
Number of successful extensions: 29467
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 29350
Number of HSP's successfully gapped: 0
Length of query: 471
Length of database: 6150218869
Length adjustment: 119
Effective length of query: 352
Effective length of database: 4017847873
Effective search space: 152678219174
Effective search space used: 152678219174
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDG8XYU01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_10508
Length=466
Score E
Sequences producing significant alignments: (Bits) Value
gb|AAF81194.1| LEA-18 [Phaseolus vulgaris] 101 3e-25
gb|AAC49859.1| PvLEA-18 [Phaseolus vulgaris] 101 5e-25
ref|NP_001238313.1| seed maturation protein PM35 [Glycine max... 100 9e-25
ref|XP_002284084.1| PREDICTED: uncharacterized protein LOC100... 100 1e-24
emb|CAN76778.1| hypothetical protein VITISV_032080 [Vitis vin... 99.4 3e-24
ALIGNMENTS
>gb|AAF81194.1| LEA-18 [Phaseolus vulgaris]
Length=82
Score = 101 bits (252), Expect = 3e-25
Identities = 54/81 (67%), Positives = 63/81 (78%), Gaps = 4/81 (5%)
Frame = -3
Query 464 EMEKKGEDK--KVNLEGLPTETSPYTQYKDLEDYKKQGYGTEGHLEPKAGHGAAGSTDAP 291
E EKK E + KVNLEGLPTE SPY +YKDLEDYK+QGYGT+GH EPK G G AG+T+AP
Sbjct 2 EKEKKTESEQGKVNLEGLPTEDSPYVKYKDLEDYKQQGYGTQGHQEPKTGRG-AGATEAP 60
Query 290 TLSGGAAADQKAQPSSTDAIN 228
TLS GAA K+Q ++T A N
Sbjct 61 TLS-GAAFSSKSQATATGATN 80
>gb|AAC49859.1| PvLEA-18 [Phaseolus vulgaris]
Length=86
Score = 101 bits (251), Expect = 5e-25
Identities = 54/81 (67%), Positives = 62/81 (77%), Gaps = 4/81 (5%)
Frame = -3
Query 464 EMEKKGEDK--KVNLEGLPTETSPYTQYKDLEDYKKQGYGTEGHLEPKAGHGAAGSTDAP 291
E EKK E + KVNLEGLPTE SPY +YKDLEDYK+QGYGT+GH EPK G G AG+T+AP
Sbjct 6 EKEKKTESEQGKVNLEGLPTEDSPYVKYKDLEDYKQQGYGTQGHQEPKTGRG-AGATEAP 64
Query 290 TLSGGAAADQKAQPSSTDAIN 228
TLS GAA K+Q + T A N
Sbjct 65 TLS-GAAFSSKSQATGTGATN 84
>ref|NP_001238313.1| seed maturation protein PM35 [Glycine max]
gb|AAD51623.1|AF169020_1 seed maturation protein PM35 [Glycine max]
gb|AAF21307.1|AF109720_1 seed maturation protein PM21 [Glycine max]
gb|ACU14932.1| unknown [Glycine max]
Length=95
Score = 100 bits (250), Expect = 9e-25
Identities = 50/76 (66%), Positives = 60/76 (79%), Gaps = 2/76 (3%)
Frame = -3
Query 440 KKVNLEGLPTETSPYTQYKDLEDYKKQGYGTEGHLEPKAGHGAAGSTDAPTLSGGAAADQ 261
K+ + EGLP E SPY +YKDLEDYK+QGYGT+GH EPK G G AG+T+APTLS GAA
Sbjct 22 KESSTEGLPMEDSPYVKYKDLEDYKRQGYGTQGHQEPKTGRG-AGATEAPTLS-GAAVSS 79
Query 260 KAQPSSTDAINKQGVP 213
AQ ++TDAIN +GVP
Sbjct 80 DAQVAATDAINSKGVP 95
>ref|XP_002284084.1| PREDICTED: uncharacterized protein LOC100244040 [Vitis vinifera]
Length=89
Score = 100 bits (249), Expect = 1e-24
Identities = 50/80 (63%), Positives = 62/80 (78%), Gaps = 3/80 (4%)
Frame = -3
Query 452 KGEDKKVNLEGLPTETSPYTQYKDLEDYKKQGYGTEGHLEPKAGHGAAGSTDAPTLSGGA 273
+ E K LEGLP ETSPYTQY DLEDYK++GYGTEGHL+PK G G+ STDAPT+S GA
Sbjct 13 ESEVKGGKLEGLPMETSPYTQYSDLEDYKRKGYGTEGHLQPKPGRGS--STDAPTIS-GA 69
Query 272 AADQKAQPSSTDAINKQGVP 213
+ +++ S+TDA N+ GVP
Sbjct 70 SVSSRSELSATDAKNRHGVP 89
>emb|CAN76778.1| hypothetical protein VITISV_032080 [Vitis vinifera]
Length=89
Score = 99.4 bits (246), Expect = 3e-24
Identities = 49/80 (61%), Positives = 62/80 (78%), Gaps = 3/80 (4%)
Frame = -3
Query 452 KGEDKKVNLEGLPTETSPYTQYKDLEDYKKQGYGTEGHLEPKAGHGAAGSTDAPTLSGGA 273
+ E+K LEGLP ETSPYTQ DLEDYK++GYGTEGHL+PK G G+ STDAPT+S GA
Sbjct 13 ESEEKGGKLEGLPMETSPYTQXSDLEDYKRKGYGTEGHLQPKPGRGS--STDAPTIS-GA 69
Query 272 AADQKAQPSSTDAINKQGVP 213
+ +++ S+TDA N+ GVP
Sbjct 70 SVSSRSELSATDAKNRHGVP 89
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 216886976
Number of extensions: 4419707
Number of successful extensions: 13226
Number of sequences better than 1e-10: 2
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 13207
Number of HSP's successfully gapped: 2
Length of query: 466
Length of database: 6150218869
Length adjustment: 117
Effective length of query: 349
Effective length of database: 4053686041
Effective search space: 154040069558
Effective search space used: 154040069558
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDHDVT101N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_10547
Length=465
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 238197342
Number of extensions: 5127797
Number of successful extensions: 15769
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 15607
Number of HSP's successfully gapped: 0
Length of query: 465
Length of database: 6150218869
Length adjustment: 117
Effective length of query: 348
Effective length of database: 4053686041
Effective search space: 154040069558
Effective search space used: 154040069558
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDHFG4601N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_10892
Length=456
Score E
Sequences producing significant alignments: (Bits) Value
dbj|BAK61860.1| hypothetical protein [Citrus unshiu] 101 9e-25
ref|XP_002514063.1| conserved hypothetical protein [Ricinus c... 101 1e-24
ref|XP_002326114.1| predicted protein [Populus trichocarpa] >... 100 2e-24
gb|ABK94669.1| unknown [Populus trichocarpa] 99.4 6e-24
gb|ABK96396.1| unknown [Populus trichocarpa x Populus deltoides] 99.0 9e-24
ALIGNMENTS
>dbj|BAK61860.1| hypothetical protein [Citrus unshiu]
Length=123
Score = 101 bits (252), Expect = 9e-25
Identities = 68/100 (68%), Positives = 80/100 (80%), Gaps = 2/100 (2%)
Frame = +2
Query 2 AFLKPLPLRSAKPSSVVLAKPAAGKQLQINASFKEKavvgltaaaltaSMIVPDVAEAAS 181
+FLKPLP+R +K + L KP + +LQ+ AS KEKAV GLTAAALTASM++P+VAEAA
Sbjct 25 SFLKPLPVRPSKAARF-LGKPKSRARLQVQASLKEKAVTGLTAAALTASMVIPEVAEAAG 83
Query 182 SA-SPSLNNFLYSIVAGGVVLTAIFGAVIGVSNFDPVKRT 298
SPSL NFL SIVAGGVVL A+ GAVIGV+NFDPVKRT
Sbjct 84 PGLSPSLKNFLLSIVAGGVVLAALVGAVIGVANFDPVKRT 123
>ref|XP_002514063.1| conserved hypothetical protein [Ricinus communis]
gb|EEF48017.1| conserved hypothetical protein [Ricinus communis]
Length=121
Score = 101 bits (251), Expect = 1e-24
Identities = 69/100 (69%), Positives = 80/100 (80%), Gaps = 5/100 (5%)
Frame = +2
Query 2 AFLKPLPLRSAKPSSVVLAKPAAGKQLQINASFKEKavvgltaaaltaSMIVPDVAEAAS 181
AF KPLPLR +K V+A + GK Q+ AS KEKA+ GLTAAALTASM++P+VAEAA
Sbjct 26 AFFKPLPLRQSK---AVMASKSNGK-FQVKASLKEKAITGLTAAALTASMVIPEVAEAAG 81
Query 182 SA-SPSLNNFLYSIVAGGVVLTAIFGAVIGVSNFDPVKRT 298
S SPSL NFL SIVAGGVVLTAI GA+IGV+NFDPVKR+
Sbjct 82 SGVSPSLKNFLLSIVAGGVVLTAIVGAIIGVANFDPVKRS 121
>ref|XP_002326114.1| predicted protein [Populus trichocarpa]
gb|EEE71784.1| predicted protein [Populus trichocarpa]
Length=121
Score = 100 bits (250), Expect = 2e-24
Identities = 68/99 (69%), Positives = 77/99 (78%), Gaps = 5/99 (5%)
Frame = +2
Query 2 AFLKPLPLRSAKPSSVVLAKPAAGKQLQINASFKEKavvgltaaaltaSMIVPDVAEAAS 181
AF KPLP A+PS + A ++G+ Q+ AS KEK V GLTA ALTASM++PDVAEAA
Sbjct 26 AFFKPLP---ARPSKAMSASKSSGR-FQVKASLKEKLVTGLTAGALTASMVIPDVAEAAG 81
Query 182 S-ASPSLNNFLYSIVAGGVVLTAIFGAVIGVSNFDPVKR 295
+ ASPSL NFL SIVAGGVVL AI GAVIGVSNFDPVKR
Sbjct 82 AGASPSLKNFLLSIVAGGVVLVAIIGAVIGVSNFDPVKR 120
>gb|ABK94669.1| unknown [Populus trichocarpa]
Length=121
Score = 99.4 bits (246), Expect = 6e-24
Identities = 67/99 (68%), Positives = 77/99 (78%), Gaps = 5/99 (5%)
Frame = +2
Query 2 AFLKPLPLRSAKPSSVVLAKPAAGKQLQINASFKEKavvgltaaaltaSMIVPDVAEAAS 181
AF KPLP A+PS + A ++G+ Q+ AS KEK V GLTA ALTASM++P+VAEAA
Sbjct 26 AFFKPLP---ARPSKAMSASKSSGR-FQVKASLKEKLVTGLTAGALTASMVIPEVAEAAG 81
Query 182 S-ASPSLNNFLYSIVAGGVVLTAIFGAVIGVSNFDPVKR 295
+ ASPSL NFL SIVAGGVVL AI GAVIGVSNFDPVKR
Sbjct 82 AGASPSLKNFLLSIVAGGVVLVAIIGAVIGVSNFDPVKR 120
>gb|ABK96396.1| unknown [Populus trichocarpa x Populus deltoides]
Length=122
Score = 99.0 bits (245), Expect = 9e-24
Identities = 63/100 (63%), Positives = 73/100 (73%), Gaps = 4/100 (4%)
Frame = +2
Query 2 AFLKPLPLRSAKPSSVVLAKPAAGKQLQINASFKEKavvgltaaaltaSMIVPDVAEAAS 181
AF KPLP A+PS + A + Q+ AS KEK V GLTA ALT SM++P+VAEAA
Sbjct 26 AFFKPLP---ARPSKAIAAASRCNDRFQVKASLKEKVVTGLTAGALTVSMVIPEVAEAAG 82
Query 182 -SASPSLNNFLYSIVAGGVVLTAIFGAVIGVSNFDPVKRT 298
SPSLNNFL SIVAGGVVL AI GAV+GVSNFDPV+R+
Sbjct 83 PGVSPSLNNFLLSIVAGGVVLVAIAGAVVGVSNFDPVRRS 122
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 216763722
Number of extensions: 4405504
Number of successful extensions: 15264
Number of sequences better than 1e-10: 4
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 15205
Number of HSP's successfully gapped: 4
Length of query: 456
Length of database: 6150218869
Length adjustment: 115
Effective length of query: 341
Effective length of database: 4089524209
Effective search space: 151312395733
Effective search space used: 151312395733
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDJHXYR01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_11012
Length=452
Score E
Sequences producing significant alignments: (Bits) Value
dbj|BAF63494.1| nodule protein Dg93-like protein [Potamogeton... 94.4 3e-22
ref|XP_002313673.1| predicted protein [Populus trichocarpa] >... 91.7 4e-21
ref|XP_002524454.1| Early nodulin, putative [Ricinus communis... 90.1 1e-20
ref|NP_001238685.1| uncharacterized protein LOC100306683 [Gly... 89.0 4e-20
gb|ABL85058.1| early nodulin protein [Brachypodium sylvaticum] 88.6 6e-20
ALIGNMENTS
>dbj|BAF63494.1| nodule protein Dg93-like protein [Potamogeton distinctus]
Length=94
Score = 94.4 bits (233), Expect = 3e-22
Identities = 62/75 (83%), Positives = 68/75 (91%), Gaps = 0/75 (0%)
Frame = -1
Query 395 AKRYSQEgakagakaaviatiatAIPTLASVKMVPWARANLNPTAQALIISTAAGMAYFI 216
AKR S EG KAG KAAV+AT+A+AIPTLASV+M+PWAR NLNPTAQALI+STAAGMAYFI
Sbjct 16 AKRCSHEGIKAGVKAAVVATVASAIPTLASVRMLPWARTNLNPTAQALIVSTAAGMAYFI 75
Query 215 VADKTILAAARNNSF 171
VADKTILA AR NSF
Sbjct 76 VADKTILATARKNSF 90
>ref|XP_002313673.1| predicted protein [Populus trichocarpa]
gb|EEE87628.1| predicted protein [Populus trichocarpa]
Length=109
Score = 91.7 bits (226), Expect = 4e-21
Identities = 62/80 (78%), Positives = 67/80 (84%), Gaps = 0/80 (0%)
Frame = -1
Query 395 AKRYSQEgakagakaaviatiatAIPTLASVKMVPWARANLNPTAQALIISTAAGMAYFI 216
AKR S EG AGAKAA +ATIATAIPTLAS +M+PWARANLNPTAQALIIST AG AYFI
Sbjct 26 AKRCSHEGVVAGAKAAALATIATAIPTLASARMLPWARANLNPTAQALIISTVAGAAYFI 85
Query 215 VADKTILAAARNNSFSGGAS 156
VADKT+LA AR NSF +S
Sbjct 86 VADKTVLATARKNSFKSRSS 105
>ref|XP_002524454.1| Early nodulin, putative [Ricinus communis]
gb|EEF37894.1| Early nodulin, putative [Ricinus communis]
Length=108
Score = 90.1 bits (222), Expect = 1e-20
Identities = 66/106 (62%), Positives = 78/106 (74%), Gaps = 7/106 (7%)
Frame = -1
Query 443 RNVSESNLEKCNPYQN-------AKRYSQEgakagakaaviatiatAIPTLASVKMVPWA 285
+NV++S LEK + AKR S EG AGAKAAV+A+IATAIPTLAS +M+PWA
Sbjct 3 KNVAQSPLEKTCSMASLDQKLAMAKRCSHEGVVAGAKAAVVASIATAIPTLASARMLPWA 62
Query 284 RANLNPTAQALIISTAAGMAYFIVADKTILAAARNNSFSGGASLSS 147
RANLN TAQALIIST AG AYFIVADKT+LA AR NSF ++ +
Sbjct 63 RANLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNIEA 108
>ref|NP_001238685.1| uncharacterized protein LOC100306683 [Glycine max]
gb|ACU14979.1| unknown [Glycine max]
Length=107
Score = 89.0 bits (219), Expect = 4e-20
Identities = 63/76 (83%), Positives = 67/76 (88%), Gaps = 0/76 (0%)
Frame = -1
Query 395 AKRYSQEgakagakaaviatiatAIPTLASVKMVPWARANLNPTAQALIISTAAGMAYFI 216
AKR S EGA AGAKAAV ATIATAIPTLASV+M+PWARANLN TAQALIIST AG AYFI
Sbjct 27 AKRCSHEGAMAGAKAAVAATIATAIPTLASVRMLPWARANLNHTAQALIISTVAGAAYFI 86
Query 215 VADKTILAAARNNSFS 168
VADKT+LA AR NSF+
Sbjct 87 VADKTVLATARKNSFN 102
>gb|ABL85058.1| early nodulin protein [Brachypodium sylvaticum]
Length=111
Score = 88.6 bits (218), Expect = 6e-20
Identities = 57/75 (76%), Positives = 66/75 (88%), Gaps = 0/75 (0%)
Frame = -1
Query 395 AKRYSQEgakagakaaviatiatAIPTLASVKMVPWARANLNPTAQALIISTAAGMAYFI 216
AKR S+E A AGAKAA +AT+A A+PTLASV+MVPWARA+LNPT QALIIST AGMAYFI
Sbjct 17 AKRCSKEAAMAGAKAAAVATVAAAVPTLASVRMVPWARAHLNPTGQALIISTVAGMAYFI 76
Query 215 VADKTILAAARNNSF 171
VADKT+L+ AR +SF
Sbjct 77 VADKTVLSMARKHSF 91
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 218115216
Number of extensions: 4370041
Number of successful extensions: 10816
Number of sequences better than 1e-10: 5
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 10804
Number of HSP's successfully gapped: 5
Length of query: 452
Length of database: 6150218869
Length adjustment: 113
Effective length of query: 339
Effective length of database: 4125362377
Effective search space: 152638407949
Effective search space used: 152638407949
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCZ2V9B01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_1122
Length=974
Score E
Sequences producing significant alignments: (Bits) Value
ref|NP_566697.1| cysteine-rich repeat secretory protein 38 [A... 176 1e-49
dbj|BAJ34612.1| unnamed protein product [Thellungiella haloph... 174 4e-49
ref|XP_002883339.1| hypothetical protein ARALYDRAFT_479720 [A... 173 9e-49
ref|XP_003543377.1| PREDICTED: cysteine-rich repeat secretory... 171 6e-48
ref|XP_003597035.1| Cysteine-rich repeat secretory protein [M... 171 9e-48
ALIGNMENTS
>ref|NP_566697.1| cysteine-rich repeat secretory protein 38 [Arabidopsis thaliana]
sp|Q9LRJ9.1|CRR38_ARATH RecName: Full=Cysteine-rich repeat secretory protein 38; Flags:
Precursor
dbj|BAB01391.1| unnamed protein product [Arabidopsis thaliana]
gb|AAK59407.1| unknown protein [Arabidopsis thaliana]
gb|ABD91491.1| At3g22060 [Arabidopsis thaliana]
gb|AEE76585.1| cysteine-rich repeat secretory protein 38 [Arabidopsis thaliana]
Length=252
Score = 176 bits (445), Expect = 1e-49
Identities = 99/228 (43%), Positives = 129/228 (57%), Gaps = 9/228 (4%)
Frame = +3
Query 66 QGSSLLNHVCTNTSQNYTKDTTYATNLKYLIDDLYVKTPQNYSFGQGSYGEGPNKVYGLA 245
Q ++ L H C++ ++T + Y +NL L L K P F S G PN V GLA
Sbjct 30 QNNAFLFHKCSDIEGSFTSKSLYESNLNNLFSQLSYKVPST-GFAASSTGNTPNNVNGLA 88
Query 246 QCRGDVSPDDCDYCLYDARCKITQRCYKRSA-VVWYDNCQLKYSDVNFLGKIDKENDFVM 422
CRGD S DC CL A ++ QRC A +VWYDNC +KYS NF GKID EN F +
Sbjct 89 LCRGDASSSDCRSCLETAIPELRQRCPNNKAGIVWYDNCLVKYSSTNFFGKIDFENRFYL 148
Query 423 INVKNVSRTEKEIFRDTSIGLLTDLSKQASDKANKYMFAGGEKLFDDKKNVTIHGMVLCT 602
NVKNVS + F + LLT+L+K+A+ + N+ +FA GEK K ++G+V CT
Sbjct 149 YNVKNVS--DPSTFNSQTKALLTELTKKATTRDNQKLFATGEKNIGKNK---LYGLVQCT 203
Query 603 QDLSYDDCKTCLDGVVKELPIYKGTIYSVGARVVGASCTVRYETYVFL 746
+DL CK CL+G++ ELP G RVVG SC RYE Y F+
Sbjct 204 RDLKSITCKACLNGIIGELP--NCCDGKEGGRVVGGSCNFRYEIYPFV 249
>dbj|BAJ34612.1| unnamed protein product [Thellungiella halophila]
Length=255
Score = 174 bits (442), Expect = 4e-49
Identities = 96/228 (42%), Positives = 131/228 (57%), Gaps = 9/228 (4%)
Frame = +3
Query 66 QGSSLLNHVCTNTSQNYTKDTTYATNLKYLIDDLYVKTPQNYSFGQGSYGEGPNKVYGLA 245
Q ++ L H C++ N+T + Y +NL L + + P + F S G P+ V GLA
Sbjct 33 QNNAFLYHKCSDIEGNFTSKSPYESNLDSLFRRISYRVPSS-GFAASSAGNSPDNVNGLA 91
Query 246 QCRGDVSPDDCDYCLYDARCKITQRCYKRSA-VVWYDNCQLKYSDVNFLGKIDKENDFVM 422
CRGD S DC CL A ++ QRC A ++WYDNC +KYS NF GKID EN F +
Sbjct 92 LCRGDASSSDCGSCLATAIPELRQRCPNNKAGIIWYDNCLVKYSSTNFFGKIDYENRFYL 151
Query 423 INVKNVSRTEKEIFRDTSIGLLTDLSKQASDKANKYMFAGGEKLFDDKKNVTIHGMVLCT 602
NV NVS + F + LLT+L+++A+ N+ +FA GEK + KK ++G+V CT
Sbjct 152 YNVNNVS--DPASFNTQTKALLTELTQKATTGDNQKLFATGEKNLEKKK---LYGLVQCT 206
Query 603 QDLSYDDCKTCLDGVVKELPIYKGTIYSVGARVVGASCTVRYETYVFL 746
+DL + CK CLDG++ ELP G RVVG SC RYE Y F+
Sbjct 207 RDLRRESCKACLDGIIGELP--NCCDGKEGGRVVGGSCNFRYEIYPFV 252
>ref|XP_002883339.1| hypothetical protein ARALYDRAFT_479720 [Arabidopsis lyrata subsp.
lyrata]
gb|EFH59598.1| hypothetical protein ARALYDRAFT_479720 [Arabidopsis lyrata subsp.
lyrata]
Length=252
Score = 173 bits (439), Expect = 9e-49
Identities = 99/229 (43%), Positives = 129/229 (56%), Gaps = 9/229 (4%)
Frame = +3
Query 66 QGSSLLNHVCTNTSQNYTKDTTYATNLKYLIDDLYVKTPQNYSFGQGSYGEGPNKVYGLA 245
Q ++ L H C++ ++T + Y +NL L L K P F S G P+ V GLA
Sbjct 30 QNNAFLFHKCSDIEGSFTSKSPYESNLNNLFPQLSYKVPST-GFATSSAGITPDNVNGLA 88
Query 246 QCRGDVSPDDCDYCLYDARCKITQRCYKRSA-VVWYDNCQLKYSDVNFLGKIDKENDFVM 422
CRGD S DC CL A +I QRC A ++WYDNC +KYS NF GKID EN F +
Sbjct 89 LCRGDASSSDCSSCLATAIPEIRQRCPSNKAGIIWYDNCLVKYSSTNFFGKIDFENRFYL 148
Query 423 INVKNVSRTEKEIFRDTSIGLLTDLSKQASDKANKYMFAGGEKLFDDKKNVTIHGMVLCT 602
NV NVS + F + LLT L+K+A+ N+ +FA GEK KK ++G+V CT
Sbjct 149 YNVNNVS--DPSTFNTQTKALLTKLTKKATTGDNQKLFATGEKNIGMKK---LYGLVQCT 203
Query 603 QDLSYDDCKTCLDGVVKELPIYKGTIYSVGARVVGASCTVRYETYVFLN 749
+DL + CK CL+G++ ELP G RVVG SC RYE Y F+N
Sbjct 204 RDLKSEACKACLNGIIGELP--NCCDGKEGGRVVGGSCNFRYEIYPFVN 250
>ref|XP_003543377.1| PREDICTED: cysteine-rich repeat secretory protein 38 [Glycine
max]
Length=244
Score = 171 bits (433), Expect = 6e-48
Identities = 99/230 (43%), Positives = 135/230 (59%), Gaps = 12/230 (5%)
Frame = +3
Query 69 GSSLLNHVCTNTSQNYTKDTTYATNLKYLIDDLYVKTPQNYSFGQGSYGEGPN-KVYGLA 245
G+ L H C+N S+N+T ++ Y +NLK LI+ L KTP FG GS G+ N K Y LA
Sbjct 25 GADPLFHFCSN-SENFTANSPYESNLKTLINSLIYKTPST-GFGVGSVGQYQNQKAYALA 82
Query 246 QCRGDVSPDDCDYCLYDARCKITQRC-YKRSAVVWYDNCQLKYSDVNFLGKIDKENDFVM 422
CRGDVS +C C+ +A +I RC Y + A++WYD C KY D +FLGKID N F M
Sbjct 83 LCRGDVSASECKTCVSEAPKEILSRCPYNKGAIIWYDYCMFKYLDTDFLGKIDNTNKFYM 142
Query 423 INVKNVSRTEKEIFRDTSIGLLTDLSKQASDKANKYMFAGGEKLFDDKKNVTIHGMVLCT 602
N+KNVS + F + LL+ L+++A N ++A GE ++ + T++G+ CT
Sbjct 143 WNLKNVS--DPATFNYNTRDLLSQLAQKA--YVNNKLYATGEAKLENSE--TLYGLTQCT 196
Query 603 QDLSYDDCKTCLDGVVKELPIYKGTIYSVGARVVGASCTVRYETYVFLND 752
+DLS DCK CLD + ELP G RVV SC RYE Y F+ +
Sbjct 197 RDLSSSDCKKCLDDAINELP--NCCDGKEGGRVVSGSCNFRYEIYPFVKE 244
>ref|XP_003597035.1| Cysteine-rich repeat secretory protein [Medicago truncatula]
gb|AES67286.1| Cysteine-rich repeat secretory protein [Medicago truncatula]
Length=246
Score = 171 bits (432), Expect = 9e-48
Identities = 100/232 (43%), Positives = 135/232 (58%), Gaps = 14/232 (6%)
Frame = +3
Query 69 GSSLLNHVCTNTSQNYTKDTTYATNLKYLIDDLYVKTPQNYSFGQGSYGEGP---NKVYG 239
G+ L H+C+ TS+N+T + Y +NLK LI+ L KTP FG GS K YG
Sbjct 25 GTDPLFHICS-TSENFTAHSPYESNLKTLINSLIYKTPST-GFGSGSIDLTQYQNQKAYG 82
Query 240 LAQCRGDVSPDDCDYCLYDARCKITQRC-YKRSAVVWYDNCQLKYSDVNFLGKIDKENDF 416
LA CRGDVS +C C+ A +I C YK+ A++WYDNC KY D +F GKID N F
Sbjct 83 LALCRGDVSTSECKTCVSQATKEILNVCPYKKGAIIWYDNCMFKYLDNDFFGKIDNTNKF 142
Query 417 VMINVKNVSRTEKEIFRDTSIGLLTDLSKQASDKANKYMFAGGEKLFDDKKNVTIHGMVL 596
++NV+NVS K F + + LL+ L+ +AS N ++A GE + + V +G+
Sbjct 143 ALLNVQNVSDPIK--FNNMTNDLLSFLANEAS--MNHKLYATGELKIGESERV--YGLTQ 196
Query 597 CTQDLSYDDCKTCLDGVVKELPIYKGTIYSVGARVVGASCTVRYETYVFLND 752
CT+D+S DCK CLDG + ELP G RVVG SC +RYE Y F+ +
Sbjct 197 CTRDISSVDCKKCLDGAISELP--NCCDGKKGGRVVGGSCNIRYEIYPFVRE 246
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 488350748
Number of extensions: 10155429
Number of successful extensions: 27066
Number of sequences better than 1e-10: 61
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 26782
Number of HSP's successfully gapped: 62
Length of query: 974
Length of database: 6150218869
Length adjustment: 140
Effective length of query: 834
Effective length of database: 3641547109
Effective search space: 670044668056
Effective search space used: 670044668056
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 177 (72.8 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDJJYNA01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_11517
Length=440
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003531875.1| PREDICTED: uncharacterized protein LOC100... 142 5e-41
ref|XP_002531002.1| conserved hypothetical protein [Ricinus c... 136 1e-38
dbj|BAC83875.1| unknown protein [Oryza sativa Japonica Group]... 132 6e-37
ref|XP_002875526.1| hypothetical protein ARALYDRAFT_323026 [A... 131 6e-37
ref|NP_189632.1| B12D protein [Arabidopsis thaliana] >dbj|BAB... 131 9e-37
ALIGNMENTS
>ref|XP_003531875.1| PREDICTED: uncharacterized protein LOC100527287 [Glycine max]
gb|ACU16358.1| unknown [Glycine max]
Length=86
Score = 142 bits (357), Expect = 5e-41
Identities = 64/83 (77%), Positives = 75/83 (90%), Gaps = 0/83 (0%)
Frame = +2
Query 5 GRWVKPEVYPLIAAMSFVTGLCVFQLSRNVIFNPDVRVNKVHRSTAVLENQEEGEKYAQH 184
GRW+KPEVYPL+AAM+FVTG+CVFQL+RNV+ NPDVR+NK RS AVLEN+EEGEKYA+H
Sbjct 2 GRWMKPEVYPLLAAMTFVTGMCVFQLTRNVLGNPDVRINKTRRSMAVLENREEGEKYAEH 61
Query 185 SLRKFLRTRTPEIMPALNRFFSQ 253
LRKFLRTR PEIMP +N FFS+
Sbjct 62 GLRKFLRTRPPEIMPTINHFFSE 84
>ref|XP_002531002.1| conserved hypothetical protein [Ricinus communis]
gb|EEF31390.1| conserved hypothetical protein [Ricinus communis]
Length=86
Score = 136 bits (342), Expect = 1e-38
Identities = 60/82 (73%), Positives = 72/82 (88%), Gaps = 0/82 (0%)
Frame = +2
Query 8 RWVKPEVYPLIAAMSFVTGLCVFQLSRNVIFNPDVRVNKVHRSTAVLENQEEGEKYAQHS 187
RW+KPEVYPL+AAM+FVT LC FQL+RN+ NPDVR+NK HR TAVLEN+ EGE+YA+H
Sbjct 3 RWIKPEVYPLMAAMTFVTSLCAFQLTRNMFLNPDVRINKAHRRTAVLENEVEGEQYAEHG 62
Query 188 LRKFLRTRTPEIMPALNRFFSQ 253
LRKFLRTR PEIMP++N FFS+
Sbjct 63 LRKFLRTRPPEIMPSINHFFSE 84
>dbj|BAC83875.1| unknown protein [Oryza sativa Japonica Group]
dbj|BAG88459.1| unnamed protein product [Oryza sativa Japonica Group]
gb|EEC82410.1| hypothetical protein OsI_26790 [Oryza sativa Indica Group]
gb|EEE67550.1| hypothetical protein OsJ_25042 [Oryza sativa Japonica Group]
Length=95
Score = 132 bits (331), Expect = 6e-37
Identities = 60/82 (73%), Positives = 71/82 (87%), Gaps = 0/82 (0%)
Frame = +2
Query 5 GRWVKPEVYPLIAAMSFVTGLCVFQLSRNVIFNPDVRVNKVHRSTAVLENQEEGEKYAQH 184
GRW+KP+VYPLIAAM+FVTGLC FQL+RNV NPDVRVNK +R +AVLEN EEGEKY QH
Sbjct 2 GRWLKPDVYPLIAAMTFVTGLCTFQLTRNVFMNPDVRVNKNNRKSAVLENAEEGEKYHQH 61
Query 185 SLRKFLRTRTPEIMPALNRFFS 250
+ R+FL T+ PE+ PALNRFF+
Sbjct 62 AFRRFLATQRPEVFPALNRFFA 83
>ref|XP_002875526.1| hypothetical protein ARALYDRAFT_323026 [Arabidopsis lyrata subsp.
lyrata]
gb|EFH51785.1| hypothetical protein ARALYDRAFT_323026 [Arabidopsis lyrata subsp.
lyrata]
Length=87
Score = 131 bits (330), Expect = 6e-37
Identities = 56/86 (65%), Positives = 73/86 (85%), Gaps = 0/86 (0%)
Frame = +2
Query 5 GRWVKPEVYPLIAAMSFVTGLCVFQLSRNVIFNPDVRVNKVHRSTAVLENQEEGEKYAQH 184
GRWV+PEVYPL+ AM FVT + VFQL+RN + NPD R+NK HR +LEN++EGEKYAQH
Sbjct 2 GRWVRPEVYPLLGAMGFVTTMVVFQLTRNALLNPDCRINKEHRKMGILENEDEGEKYAQH 61
Query 185 SLRKFLRTRTPEIMPALNRFFSQKND 262
+ RK+LRTR P++MP+LNRFFSQ+++
Sbjct 62 NFRKYLRTRQPQVMPSLNRFFSQEDN 87
>ref|NP_189632.1| B12D protein [Arabidopsis thaliana]
dbj|BAB02998.1| unnamed protein product [Arabidopsis thaliana]
gb|AEE77618.1| B12D protein [Arabidopsis thaliana]
Length=87
Score = 131 bits (329), Expect = 9e-37
Identities = 56/86 (65%), Positives = 72/86 (84%), Gaps = 0/86 (0%)
Frame = +2
Query 5 GRWVKPEVYPLIAAMSFVTGLCVFQLSRNVIFNPDVRVNKVHRSTAVLENQEEGEKYAQH 184
GRWV+PEVYPL+ AM FVT + VFQL+RN NPD R+NK HR +LEN++EGEKYAQH
Sbjct 2 GRWVRPEVYPLLGAMGFVTSMVVFQLTRNAFLNPDCRINKEHRKMGILENKDEGEKYAQH 61
Query 185 SLRKFLRTRTPEIMPALNRFFSQKND 262
+ RK+LRTR P++MP+LNRFFSQ+++
Sbjct 62 NFRKYLRTRQPQVMPSLNRFFSQEDN 87
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 206958538
Number of extensions: 3808331
Number of successful extensions: 7505
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 7505
Number of HSP's successfully gapped: 0
Length of query: 440
Length of database: 6150218869
Length adjustment: 110
Effective length of query: 330
Effective length of database: 4179119629
Effective search space: 150448306644
Effective search space used: 150448306644
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDJN51T01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_11956
Length=430
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002311334.1| predicted protein [Populus trichocarpa] >... 67.0 2e-18
ref|XP_003528855.1| PREDICTED: protein KTI12 homolog [Glycine... 66.2 4e-18
emb|CBI27263.3| unnamed protein product [Vitis vinifera] 62.4 8e-17
emb|CAN64163.1| hypothetical protein VITISV_040646 [Vitis vin... 62.4 8e-17
ref|XP_002271192.1| PREDICTED: protein KTI12 homolog [Vitis v... 62.4 8e-17
ALIGNMENTS
>ref|XP_002311334.1| predicted protein [Populus trichocarpa]
gb|EEE88701.1| predicted protein [Populus trichocarpa]
Length=302
Score = 67.0 bits (162), Expect(2) = 2e-18
Identities = 30/39 (77%), Positives = 36/39 (92%), Gaps = 0/39 (0%)
Frame = -3
Query 428 LGGPLNGVSIGPGLPSVGISRPVGLPELRRLRRTFIKLT 312
+GGPLNG+S+G GLP++ +SR VGLPELRRLRRTFIKLT
Sbjct 232 IGGPLNGISLGQGLPTLNMSRSVGLPELRRLRRTFIKLT 270
Score = 49.7 bits (117), Expect(2) = 2e-18
Identities = 22/23 (96%), Positives = 22/23 (96%), Gaps = 0/23 (0%)
Frame = -1
Query 256 PPPPSDATSAKRMFVDYLNRELG 188
PPPPSDA SAKRMFVDYLNRELG
Sbjct 278 PPPPSDAESAKRMFVDYLNRELG 300
>ref|XP_003528855.1| PREDICTED: protein KTI12 homolog [Glycine max]
Length=302
Score = 66.2 bits (160), Expect(2) = 4e-18
Identities = 32/39 (82%), Positives = 34/39 (87%), Gaps = 0/39 (0%)
Frame = -3
Query 428 LGGPLNGVSIGPGLPSVGISRPVGLPELRRLRRTFIKLT 312
LGGPLNGVSIG LP + ISR VGLPELRR+RRTFIKLT
Sbjct 232 LGGPLNGVSIGKDLPVINISRSVGLPELRRMRRTFIKLT 270
Score = 49.3 bits (116), Expect(2) = 4e-18
Identities = 21/23 (91%), Positives = 22/23 (96%), Gaps = 0/23 (0%)
Frame = -1
Query 256 PPPPSDATSAKRMFVDYLNRELG 188
PPPPSDA SAKRMF+DYLNRELG
Sbjct 278 PPPPSDADSAKRMFIDYLNRELG 300
>emb|CBI27263.3| unnamed protein product [Vitis vinifera]
Length=621
Score = 62.4 bits (150), Expect(2) = 8e-17
Identities = 28/39 (72%), Positives = 34/39 (87%), Gaps = 0/39 (0%)
Frame = -3
Query 428 LGGPLNGVSIGPGLPSVGISRPVGLPELRRLRRTFIKLT 312
+GGP+NG+S+G GL + IS+ VGLPELRRLRRTFIKLT
Sbjct 551 VGGPVNGISLGQGLAPINISKSVGLPELRRLRRTFIKLT 589
Score = 48.9 bits (115), Expect(2) = 8e-17
Identities = 21/24 (88%), Positives = 23/24 (96%), Gaps = 0/24 (0%)
Frame = -1
Query 256 PPPPSDATSAKRMFVDYLNRELGN 185
PPPPSD+ SAKRMFVDYLNRELG+
Sbjct 597 PPPPSDSDSAKRMFVDYLNRELGS 620
>emb|CAN64163.1| hypothetical protein VITISV_040646 [Vitis vinifera]
Length=601
Score = 62.4 bits (150), Expect(2) = 8e-17
Identities = 28/39 (72%), Positives = 34/39 (87%), Gaps = 0/39 (0%)
Frame = -3
Query 428 LGGPLNGVSIGPGLPSVGISRPVGLPELRRLRRTFIKLT 312
+GGP+NG+S+G GL + IS+ VGLPELRRLRRTFIKLT
Sbjct 531 VGGPVNGISLGQGLAPINISKSVGLPELRRLRRTFIKLT 569
Score = 48.9 bits (115), Expect(2) = 8e-17
Identities = 21/24 (88%), Positives = 23/24 (96%), Gaps = 0/24 (0%)
Frame = -1
Query 256 PPPPSDATSAKRMFVDYLNRELGN 185
PPPPSD+ SAKRMFVDYLNRELG+
Sbjct 577 PPPPSDSDSAKRMFVDYLNRELGS 600
>ref|XP_002271192.1| PREDICTED: protein KTI12 homolog [Vitis vinifera]
Length=302
Score = 62.4 bits (150), Expect(2) = 8e-17
Identities = 28/39 (72%), Positives = 34/39 (87%), Gaps = 0/39 (0%)
Frame = -3
Query 428 LGGPLNGVSIGPGLPSVGISRPVGLPELRRLRRTFIKLT 312
+GGP+NG+S+G GL + IS+ VGLPELRRLRRTFIKLT
Sbjct 232 VGGPVNGISLGQGLAPINISKSVGLPELRRLRRTFIKLT 270
Score = 48.9 bits (115), Expect(2) = 8e-17
Identities = 21/24 (88%), Positives = 23/24 (96%), Gaps = 0/24 (0%)
Frame = -1
Query 256 PPPPSDATSAKRMFVDYLNRELGN 185
PPPPSD+ SAKRMFVDYLNRELG+
Sbjct 278 PPPPSDSDSAKRMFVDYLNRELGS 301
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 218743152
Number of extensions: 4793444
Number of successful extensions: 12934
Number of sequences better than 1e-10: 2
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 12914
Number of HSP's successfully gapped: 4
Length of query: 430
Length of database: 6150218869
Length adjustment: 107
Effective length of query: 323
Effective length of database: 4232876881
Effective search space: 152383567716
Effective search space used: 152383567716
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD01A3Y016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_1258
Length=954
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002510959.1| zinc/iron transporter, putative [Ricinus ... 400 1e-135
gb|AAT01414.1| iron regulated transporter [Cucumis sativus] 399 1e-135
dbj|BAF48330.1| iron transporter protein IRT1 [Nicotiana taba... 396 4e-134
ref|NP_001234252.1| iron-regulated transporter 2 precursor [S... 394 1e-133
ref|XP_002273179.1| PREDICTED: probable zinc transporter 10 [... 382 8e-129
ALIGNMENTS
>ref|XP_002510959.1| zinc/iron transporter, putative [Ricinus communis]
gb|EEF51561.1| zinc/iron transporter, putative [Ricinus communis]
Length=350
Score = 400 bits (1027), Expect = 1e-135
Identities = 224/275 (81%), Positives = 237/275 (86%), Gaps = 6/275 (2%)
Frame = -2
Query 953 LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTLIID 774
LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTL++D
Sbjct 79 LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTLLVD 138
Query 773 SMATSLYSKKHQA-VLPESD--GGNREMAEVEggdgghghfhghhhGAKINAGPQLLRYR 603
SMATS+YSKK V PE++ +REM V G +K G QLLRYR
Sbjct 139 SMATSIYSKKCSVGVNPENELVQQDREMGTVNARQGHSHGHFHA---SKATDGQQLLRYR 195
Query 602 VIAMVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYkfi 423
VIAMVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYK
Sbjct 196 VIAMVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYKLF 255
Query 422 kkaamafffAVTTPFGIALGMALSSTYKENSPRSLIVVGLLNASSAGLLIYMALVDLLAA 243
KK MAFFF+VTTPFGIALG+ALS TYKENSP +LI VGLLNASSAGLLIYMALVDLLAA
Sbjct 256 KKVMMAFFFSVTTPFGIALGIALSKTYKENSPTALITVGLLNASSAGLLIYMALVDLLAA 315
Query 242 DFMGPKLQGSVKLQIKSYAAVLLGAGGMSLMAKWA 138
DFMGPKLQGS++LQIKSY AVLLGAGGMS+MAKWA
Sbjct 316 DFMGPKLQGSIRLQIKSYIAVLLGAGGMSVMAKWA 350
>gb|AAT01414.1| iron regulated transporter [Cucumis sativus]
Length=350
Score = 399 bits (1026), Expect = 1e-135
Identities = 215/272 (79%), Positives = 243/272 (89%), Gaps = 0/272 (0%)
Frame = -2
Query 953 LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTLIID 774
+FVI+KAFAAGIILATGFMHVLPDSFDMLWS+CLKENPWHKFPF+GFVAM+SAIVTL++D
Sbjct 79 MFVILKAFAAGIILATGFMHVLPDSFDMLWSNCLKENPWHKFPFSGFVAMMSAIVTLMVD 138
Query 773 SMATSLYSKKHQAVLPESDGGNREMAEVEggdgghghfhghhhGAKINAGPQLLRYRVIA 594
SMATSLY+KKH V+PE+ + E+ GGH H H H + NAG QLLRYRV+A
Sbjct 139 SMATSLYTKKHNEVMPENSPRGGDDHELPVVSGGHFHGHHHMDTKETNAGSQLLRYRVVA 198
Query 593 MVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYkfikka 414
MVLELGI+VHS+VIGLSLGA+N+TC+IKGLVAALCFHQMFEGMGLGGCILQAEYK++KKA
Sbjct 199 MVLELGIVVHSVVIGLSLGATNDTCTIKGLVAALCFHQMFEGMGLGGCILQAEYKWMKKA 258
Query 413 amafffAVTTPFGIALGMALSSTYKENSPRSLIVVGLLNASSAGLLIYMALVDLLAADFM 234
M FFF+VTTPFGIALG+ LS TYKENSP +L+ VGLLNASSAGLLIYMALVDLL+ADFM
Sbjct 259 IMVFFFSVTTPFGIALGIGLSKTYKENSPVALVTVGLLNASSAGLLIYMALVDLLSADFM 318
Query 233 GPKLQGSVKLQIKSYAAVLLGAGGMSLMAKWA 138
GPKLQGS+KLQ+KSY AVLLGAGGMSLMAKWA
Sbjct 319 GPKLQGSIKLQVKSYIAVLLGAGGMSLMAKWA 350
>dbj|BAF48330.1| iron transporter protein IRT1 [Nicotiana tabacum]
Length=355
Score = 396 bits (1017), Expect = 4e-134
Identities = 230/276 (83%), Positives = 244/276 (88%), Gaps = 4/276 (1%)
Frame = -2
Query 953 LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTLIID 774
LFVIVKAFAAGIILATGFMHVLPDSFDML S CLKENPWHKFPFTGFVAMLSAI TL ID
Sbjct 80 LFVIVKAFAAGIILATGFMHVLPDSFDMLSSSCLKENPWHKFPFTGFVAMLSAIFTLAID 139
Query 773 SMATSLYSKKHQA-VLPESDG--GNREMAEVEggdgghghfhghhhGAKINA-GPQLLRY 606
SMATSLYSKK++A V+PES G++EM V G+ H H H K G +LLRY
Sbjct 140 SMATSLYSKKNKAGVIPESQSQDGDQEMGAVNAGNHVHSHHHHGSFSTKDGVDGAKLLRY 199
Query 605 RVIAMVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYkf 426
RVIAMVLELGIIVHSIVIGLSLGASNNTC+IKGLVAALCFHQMFEGMGLGGCILQAEYKF
Sbjct 200 RVIAMVLELGIIVHSIVIGLSLGASNNTCTIKGLVAALCFHQMFEGMGLGGCILQAEYKF 259
Query 425 ikkaamafffAVTTPFGIALGMALSSTYKENSPRSLIVVGLLNASSAGLLIYMALVDLLA 246
+KKA MAFFFA+TTPFGIALG+ALSSTY+ENSPR+LI VGLLNASSAGLLIYMALVDLLA
Sbjct 260 LKKAIMAFFFAITTPFGIALGIALSSTYEENSPRALITVGLLNASSAGLLIYMALVDLLA 319
Query 245 ADFMGPKLQGSVKLQIKSYAAVLLGAGGMSLMAKWA 138
ADFMG KLQGS+KLQIKSY AVLLGAGGMSLMAKWA
Sbjct 320 ADFMGDKLQGSIKLQIKSYMAVLLGAGGMSLMAKWA 355
>ref|NP_001234252.1| iron-regulated transporter 2 precursor [Solanum lycopersicum]
gb|AAD30549.1|AF136580_1 iron-regulated transporter 2 [Solanum lycopersicum]
gb|AAF97510.1|AF246266_2 iron-regulated transporter 2 [Solanum lycopersicum]
Length=352
Score = 394 bits (1013), Expect = 1e-133
Identities = 226/274 (82%), Positives = 240/274 (88%), Gaps = 4/274 (1%)
Frame = -2
Query 953 LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTLIID 774
LFVIVKAFAAGIILATGFMHVLPDSFDML S CLKENPWHKFPFTGFVAMLSAIVT+ ID
Sbjct 81 LFVIVKAFAAGIILATGFMHVLPDSFDMLSSSCLKENPWHKFPFTGFVAMLSAIVTMAID 140
Query 773 SMATSLYSKKHQAVL--PESDGGNREMAEVEggdgghghfhghhhGAKINAGPQLLRYRV 600
S+ATS+YSKKH+A L PE+ G ++EM V GG H H G +LLRYRV
Sbjct 141 SIATSMYSKKHRAGLVNPETGGADQEMGAVNGGHSHHHHGSLSTKDGV--EGTKLLRYRV 198
Query 599 IAMVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYkfik 420
IAMVLELGIIVHSIVIG+SLGASNNTC+IKGLVAALCFHQMFEGMGLGGCILQAEYKF+K
Sbjct 199 IAMVLELGIIVHSIVIGISLGASNNTCTIKGLVAALCFHQMFEGMGLGGCILQAEYKFLK 258
Query 419 kaamafffAVTTPFGIALGMALSSTYKENSPRSLIVVGLLNASSAGLLIYMALVDLLAAD 240
K MAFFFAVTTPFGIALGMALS+TY+E SPR+LI VGLLNASSAGLLIYMALVDLLAAD
Sbjct 259 KTLMAFFFAVTTPFGIALGMALSTTYEETSPRALITVGLLNASSAGLLIYMALVDLLAAD 318
Query 239 FMGPKLQGSVKLQIKSYAAVLLGAGGMSLMAKWA 138
FMG KLQGSVKLQIKSY AVLLGAGGMSLMAKWA
Sbjct 319 FMGDKLQGSVKLQIKSYMAVLLGAGGMSLMAKWA 352
>ref|XP_002273179.1| PREDICTED: probable zinc transporter 10 [Vitis vinifera]
emb|CAN72233.1| hypothetical protein VITISV_032803 [Vitis vinifera]
emb|CBI29061.3| unnamed protein product [Vitis vinifera]
Length=348
Score = 382 bits (981), Expect = 8e-129
Identities = 214/273 (78%), Positives = 238/273 (87%), Gaps = 6/273 (2%)
Frame = -2
Query 953 LFVIVKAFAAGIILATGFMHVLPDSFDMLWSDCLKENPWHKFPFTGFVAMLSAIVTLIID 774
LF+IVKAFA+GIILATGFMHVLPDSFDMLWS CLKENPWHKFPFTGFVAMLSAI TL++D
Sbjct 81 LFIIVKAFASGIILATGFMHVLPDSFDMLWSPCLKENPWHKFPFTGFVAMLSAIFTLMVD 140
Query 773 SMATSLYSKKHQA-VLPESDGGNREMAEVEggdgghghfhghhhGAKINAGPQLLRYRVI 597
S+ATSLY+KK+ ++PE E+A++ G+ G HH G QLLRYRV+
Sbjct 141 SIATSLYTKKNNTGIIPEI-----EVADMAAGNTGGHFHGHHHGPKIGIEGSQLLRYRVV 195
Query 596 AMVLELGIIVHSIVIGLSLGASNNTCSIKGLVAALCFHQMFEGMGLGGCILQAEYkfikk 417
AMVLELGI+VHSIVIGLS+GASNNTC+IK LVAALCFHQMFEGMGLGGCILQAEYKF+KK
Sbjct 196 AMVLELGIVVHSIVIGLSMGASNNTCTIKPLVAALCFHQMFEGMGLGGCILQAEYKFVKK 255
Query 416 aamafffAVTTPFGIALGMALSSTYKENSPRSLIVVGLLNASSAGLLIYMALVDLLAADF 237
A M FFF+VTTPFGIALG+ALS TYKENSP SLI VGLLNASSAGLLIYMALVDLL+ADF
Sbjct 256 AWMVFFFSVTTPFGIALGIALSKTYKENSPTSLISVGLLNASSAGLLIYMALVDLLSADF 315
Query 236 MGPKLQGSVKLQIKSYAAVLLGAGGMSLMAKWA 138
MGPKLQGS+KLQIKS+ AVLLGAGGMS+MAKWA
Sbjct 316 MGPKLQGSIKLQIKSFVAVLLGAGGMSVMAKWA 348
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 10544852553
Number of extensions: 233317564
Number of successful extensions: 558658
Number of sequences better than 1e-10: 767
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 556595
Number of HSP's successfully gapped: 858
Length of query: 954
Length of database: 6150218869
Length adjustment: 140
Effective length of query: 814
Effective length of database: 3641547109
Effective search space: 648195385402
Effective search space used: 648195385402
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 177 (72.8 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDMNAEM01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_12610
Length=415
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002533352.1| conserved hypothetical protein [Ricinus c... 75.5 4e-15
ref|XP_003523185.1| PREDICTED: uncharacterized protein LOC100... 71.6 1e-13
ref|NP_001235543.1| uncharacterized protein LOC100527144 [Gly... 71.6 1e-13
ref|NP_191547.2| uncharacterized protein [Arabidopsis thalian... 66.6 9e-12
ref|XP_003602727.1| hypothetical protein MTR_3g098390 [Medica... 64.3 4e-11
ALIGNMENTS
>ref|XP_002533352.1| conserved hypothetical protein [Ricinus communis]
gb|EEF29037.1| conserved hypothetical protein [Ricinus communis]
Length=112
Score = 75.5 bits (184), Expect = 4e-15
Identities = 46/115 (40%), Positives = 63/115 (55%), Gaps = 7/115 (6%)
Frame = -2
Query 369 MDGLIPMVYKSLKRSKTNRHYQCLSPSSAAPITHTYNISDFYAKN-ETLMRR--EIDDQY 199
M+GL+P+VYK++KR++T R Y+CLS +A YN +DFY + ET + I +
Sbjct 1 MEGLLPLVYKAIKRNRTRRQYECLSSGAA----FAYNPADFYISDAETTYTKPSSIIMEK 56
Query 198 VNKASGIHGAHHRRYNSVHVVDRRAFDLEEVDYEAAKPKQLVRFRSHRMFSCVNG 34
N +G H R Y+SV + + KQLVRFRS RMFSCV G
Sbjct 57 NNAGTGRSKLHRRSYSSVEDLSVTGSSRRRTAGASPPRKQLVRFRSQRMFSCVTG 111
>ref|XP_003523185.1| PREDICTED: uncharacterized protein LOC100803290 [Glycine max]
Length=115
Score = 71.6 bits (174), Expect = 1e-13
Identities = 41/116 (35%), Positives = 63/116 (54%), Gaps = 6/116 (5%)
Frame = -2
Query 369 MDGLIPMVYKSLKRSKTNRHYQCLSPSSAAPITHTYNISDFY--AKNETLMRREIDDQYV 196
M+GL+P+VYK++KR+KT R Y+CL SS + + ++ + Y ++ L + +
Sbjct 1 MEGLLPLVYKAIKRNKTRRQYECL--SSGTSLGYNISMPEMYPQTQDHVLQNQTHTQKMF 58
Query 195 NKASGIHGAHHRRYNSVHVVDRRAFDLEEV--DYEAAKPKQLVRFRSHRMFSCVNG 34
+ H HRRYNSV F ++ +A +LVRFRS RMFSC+ G
Sbjct 59 HHPEEGHKVGHRRYNSVDDHFNYGFQSAQMRTGVDAPSSNKLVRFRSQRMFSCITG 114
>ref|NP_001235543.1| uncharacterized protein LOC100527144 [Glycine max]
gb|ACU16193.1| unknown [Glycine max]
Length=116
Score = 71.6 bits (174), Expect = 1e-13
Identities = 43/117 (37%), Positives = 68/117 (58%), Gaps = 7/117 (6%)
Frame = -2
Query 369 MDGLIPMVYKSLKRSKTNRHYQCLSPSSAAPITHTYNISDFYAK-NETLMRREIDDQYV- 196
M+GL+P+VYK++KR+KT R Y+CL SS + + ++++ Y + E +++ + Q V
Sbjct 1 MEGLLPLVYKAIKRNKTRRQYECL--SSGTSLGYNISMAEMYPQTQEHVLQNQTHAQKVF 58
Query 195 -NKASGIHGAHHRRYNSVHVVDRRAFDLEEV--DYEAAKPKQLVRFRSHRMFSCVNG 34
+ + H HRRYNSV F ++ +A +LVRFRS RMFSC+ G
Sbjct 59 HHHHNESHKIGHRRYNSVGDHFDYGFQSSQMRTGVDAPSSNKLVRFRSQRMFSCITG 115
>ref|NP_191547.2| uncharacterized protein [Arabidopsis thaliana]
gb|AAX23876.1| hypothetical protein At3g59880 [Arabidopsis thaliana]
gb|AAZ52732.1| expressed protein [Arabidopsis thaliana]
gb|AEE79980.1| uncharacterized protein [Arabidopsis thaliana]
Length=125
Score = 66.6 bits (161), Expect = 9e-12
Identities = 44/126 (35%), Positives = 72/126 (57%), Gaps = 16/126 (13%)
Frame = -2
Query 369 MDGLIPMVYKSLKRSKTNRHYQCLSPSSAAPITHTYNISDFY---AKNE-TLMRR---EI 211
MDGLIPM +K++++++T + Y+CLS SSA +Y+ SDF+ AK++ +++ R
Sbjct 1 MDGLIPMAFKAMRKNRTRKRYECLS-SSAGTTKDSYDDSDFFPFAAKSDHSVVSRPSSSF 59
Query 210 DDQYVNKASGIHGAHHRRYN-------SVHVVDRRAFDLEEVDYEAAKPKQLVRFRSHRM 52
D +N + HHR ++ S R D ++ + ++ QLVR RSHR+
Sbjct 60 DHIEMNNVD-VQQRHHRGWSVGDFSSMSCREGKRSGVDGGDIGFSPSRRGQLVRNRSHRL 118
Query 51 FSCVNG 34
FSCV+G
Sbjct 119 FSCVSG 124
>ref|XP_003602727.1| hypothetical protein MTR_3g098390 [Medicago truncatula]
gb|ACJ83844.1| unknown [Medicago truncatula]
gb|AES72978.1| hypothetical protein MTR_3g098390 [Medicago truncatula]
Length=99
Score = 64.3 bits (155), Expect = 4e-11
Identities = 41/115 (36%), Positives = 59/115 (51%), Gaps = 18/115 (16%)
Frame = -2
Query 369 MDGLIPMVYKSLKRSKTNRHYQCLSPSSAAPITHTYNISDFYAKNETLMRREIDDQYVNK 190
M+GL+P+VYK++K+++T R Y+ +S + +YNIS EI Q
Sbjct 1 MEGLLPLVYKAIKKNRTRRQYEVIS------LGGSYNIS----------MAEIHPQTQQP 44
Query 189 ASGIHGAHHRRYNSVHVVDRRAFDLEE--VDYEAAKPKQLVRFRSHRMFSCVNGA 31
AS + HRR+ SV F + + K+LVRF SHRMFSC+ GA
Sbjct 45 ASDDYNIGHRRHKSVGDSFGNGFQFHDQTTTGSVSPSKKLVRFSSHRMFSCIKGA 99
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 194510190
Number of extensions: 3793014
Number of successful extensions: 11432
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 11428
Number of HSP's successfully gapped: 0
Length of query: 415
Length of database: 6150218869
Length adjustment: 103
Effective length of query: 312
Effective length of database: 4304553217
Effective search space: 150659362595
Effective search space used: 150659362595
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDMRJF1012
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_12720
Length=413
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002529408.1| Hydrophobic protein OSR8, putative [Ricin... 112 2e-29
gb|ABK94331.1| unknown [Populus trichocarpa] 112 2e-29
ref|XP_002277561.1| PREDICTED: UPF0057 membrane protein At4g3... 110 6e-29
ref|XP_003519155.1| PREDICTED: UPF0057 membrane protein At4g3... 110 6e-29
ref|NP_974629.1| Low temperature and salt responsive protein ... 110 6e-29
ALIGNMENTS
>ref|XP_002529408.1| Hydrophobic protein OSR8, putative [Ricinus communis]
gb|EEF33004.1| Hydrophobic protein OSR8, putative [Ricinus communis]
Length=75
Score = 112 bits (279), Expect = 2e-29
Identities = 50/62 (81%), Positives = 58/62 (94%), Gaps = 0/62 (0%)
Frame = -2
Query 370 MASKCEIFCEIILAILLPPLGVCLRHGCCTVEVLICLILTILGYVPGIIYALYAILFMDR 191
M SKCEI CEI++AILLPPLGVCLRHGCC+VE ICL+LTILGYVPGIIYALYAI+F++R
Sbjct 1 MPSKCEILCEILIAILLPPLGVCLRHGCCSVEFWICLLLTILGYVPGIIYALYAIVFVNR 60
Query 190 DK 185
D+
Sbjct 61 DE 62
>gb|ABK94331.1| unknown [Populus trichocarpa]
Length=77
Score = 112 bits (279), Expect = 2e-29
Identities = 50/63 (79%), Positives = 58/63 (92%), Gaps = 0/63 (0%)
Frame = -2
Query 370 MASKCEIFCEIILAILLPPLGVCLRHGCCTVEVLICLILTILGYVPGIIYALYAILFMDR 191
M S+CEI CEII+AILLPPLGVC RHGCC+VE ICL+LTILGYVPGIIYALYAI+F+DR
Sbjct 1 MPSRCEICCEIIIAILLPPLGVCFRHGCCSVEFWICLLLTILGYVPGIIYALYAIVFIDR 60
Query 190 DKH 182
D++
Sbjct 61 DEY 63
>ref|XP_002277561.1| PREDICTED: UPF0057 membrane protein At4g30650 [Vitis vinifera]
emb|CAN64139.1| hypothetical protein VITISV_004807 [Vitis vinifera]
emb|CAN73911.1| hypothetical protein VITISV_002031 [Vitis vinifera]
emb|CBI36543.3| unnamed protein product [Vitis vinifera]
Length=72
Score = 110 bits (275), Expect = 6e-29
Identities = 46/61 (75%), Positives = 57/61 (93%), Gaps = 0/61 (0%)
Frame = -2
Query 370 MASKCEIFCEIILAILLPPLGVCLRHGCCTVEVLICLILTILGYVPGIIYALYAILFMDR 191
MA++CEIFCEI++AILLPP+GVC RHGCC+VE ICL+LT+LGY+PGIIYALY I+F+DR
Sbjct 1 MATRCEIFCEILIAILLPPVGVCFRHGCCSVEFCICLLLTLLGYIPGIIYALYVIVFVDR 60
Query 190 D 188
D
Sbjct 61 D 61
>ref|XP_003519155.1| PREDICTED: UPF0057 membrane protein At4g30660 [Glycine max]
gb|ACU14597.1| unknown [Glycine max]
Length=75
Score = 110 bits (275), Expect = 6e-29
Identities = 46/63 (73%), Positives = 59/63 (94%), Gaps = 0/63 (0%)
Frame = -2
Query 370 MASKCEIFCEIILAILLPPLGVCLRHGCCTVEVLICLILTILGYVPGIIYALYAILFMDR 191
M ++CEI CEI++AIL+PPLGVC RHGCC+VE +ICL+LTILGY+PGIIYALYAI+F+DR
Sbjct 1 MPTRCEICCEIMIAILIPPLGVCFRHGCCSVEFIICLLLTILGYIPGIIYALYAIIFVDR 60
Query 190 DKH 182
D++
Sbjct 61 DQY 63
>ref|NP_974629.1| Low temperature and salt responsive protein [Arabidopsis thaliana]
ref|XP_002867468.1| hypothetical protein ARALYDRAFT_328884 [Arabidopsis lyrata subsp.
lyrata]
gb|EFH43727.1| hypothetical protein ARALYDRAFT_328884 [Arabidopsis lyrata subsp.
lyrata]
gb|AEE85439.1| Low temperature and salt responsive protein [Arabidopsis thaliana]
Length=77
Score = 110 bits (275), Expect = 6e-29
Identities = 48/62 (77%), Positives = 58/62 (94%), Gaps = 0/62 (0%)
Frame = -2
Query 370 MASKCEIFCEIILAILLPPLGVCLRHGCCTVEVLICLILTILGYVPGIIYALYAILFMDR 191
MA+ CEI CEI++AIL+PPLGVCLRHGCCT E +ICLILT+LGYVPGIIYALYAI+++DR
Sbjct 1 MANGCEICCEIMIAILIPPLGVCLRHGCCTTEFMICLILTLLGYVPGIIYALYAIVYVDR 60
Query 190 DK 185
D+
Sbjct 61 DQ 62
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 4280028584
Number of extensions: 89772719
Number of successful extensions: 237462
Number of sequences better than 1e-10: 135
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 235554
Number of HSP's successfully gapped: 135
Length of query: 413
Length of database: 6150218869
Length adjustment: 102
Effective length of query: 311
Effective length of database: 4322472301
Effective search space: 151286530535
Effective search space used: 151286530535
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDNS6CM016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_12962
Length=408
Score E
Sequences producing significant alignments: (Bits) Value
emb|CAA96570.1| CP12 [Pisum sativum] 135 4e-38
gb|AAV63570.1| auxin-induced putative CP12 domain-containing ... 131 3e-37
ref|XP_003625969.1| CP12 [Medicago truncatula] >gb|AES82187.1... 132 1e-36
ref|NP_566100.2| CP12 domain-containing protein 1 [Arabidopsi... 131 2e-36
ref|XP_002880299.1| cp12 domain-containing protein 1 [Arabido... 129 1e-35
ALIGNMENTS
>emb|CAA96570.1| CP12 [Pisum sativum]
Length=127
Score = 135 bits (340), Expect = 4e-38
Identities = 61/73 (84%), Positives = 69/73 (95%), Gaps = 0/73 (0%)
Frame = +1
Query 1 PDQISGKVEESIKNAKETCSDDPVSGECAAAWDEVEELSAAASHARDKQKGSDPLENYCN 180
P+QIS KVEESIK+A+ETC+DDPVSGEC AAWDEVEELSAAASHARD++K SDPLE+YC
Sbjct 55 PEQISKKVEESIKSAQETCADDPVSGECVAAWDEVEELSAAASHARDRKKESDPLEDYCK 114
Query 181 DNPETDECRTYDN 219
DNPETDEC+TYDN
Sbjct 115 DNPETDECKTYDN 127
>gb|AAV63570.1| auxin-induced putative CP12 domain-containing protein [Arachis
hypogaea]
Length=73
Score = 131 bits (330), Expect = 3e-37
Identities = 60/73 (82%), Positives = 66/73 (90%), Gaps = 0/73 (0%)
Frame = +1
Query 1 PDQISGKVEESIKNAKETCSDDPVSGECAAAWDEVEELSAAASHARDKQKGSDPLENYCN 180
PD+IS KVE+SIK A+E C+D+P S ECAAAWDEVEELSAAASHARDK+K SDPLENYC
Sbjct 1 PDKISEKVEKSIKEAEEACTDNPASNECAAAWDEVEELSAAASHARDKKKESDPLENYCK 60
Query 181 DNPETDECRTYDN 219
DNPETDECRTYDN
Sbjct 61 DNPETDECRTYDN 73
>ref|XP_003625969.1| CP12 [Medicago truncatula]
gb|AES82187.1| CP12 [Medicago truncatula]
Length=128
Score = 132 bits (331), Expect = 1e-36
Identities = 59/72 (82%), Positives = 68/72 (94%), Gaps = 0/72 (0%)
Frame = +1
Query 1 PDQISGKVEESIKNAKETCSDDPVSGECAAAWDEVEELSAAASHARDKQKGSDPLENYCN 180
P+QIS KVEESIK+A+ETC+DDPVSGEC AAWDEVEELSAAASHARD++K SDPLE+YC
Sbjct 56 PEQISKKVEESIKSAQETCADDPVSGECVAAWDEVEELSAAASHARDRKKDSDPLEDYCK 115
Query 181 DNPETDECRTYD 216
DNPETDEC+T+D
Sbjct 116 DNPETDECKTFD 127
>ref|NP_566100.2| CP12 domain-containing protein 1 [Arabidopsis thaliana]
gb|AAL32917.1| putative chloroplast protein CP12 [Arabidopsis thaliana]
gb|AAM47914.1| putative chloroplast protein CP12 [Arabidopsis thaliana]
gb|AEC10836.1| CP12 domain-containing protein 1 [Arabidopsis thaliana]
Length=124
Score = 131 bits (329), Expect = 2e-36
Identities = 62/73 (85%), Positives = 67/73 (92%), Gaps = 2/73 (3%)
Frame = +1
Query 7 QISGKVEESIKNAKETCSDDPVSGECAAAWDEVEELSAAASHARDKQK--GSDPLENYCN 180
+IS KVE+SI+ AKETC+DDPVSGEC AAWDEVEELSAAASHARDK+K GSDPLE YCN
Sbjct 52 EISEKVEKSIQEAKETCADDPVSGECVAAWDEVEELSAAASHARDKKKAGGSDPLEEYCN 111
Query 181 DNPETDECRTYDN 219
DNPETDECRTYDN
Sbjct 112 DNPETDECRTYDN 124
>ref|XP_002880299.1| cp12 domain-containing protein 1 [Arabidopsis lyrata subsp. lyrata]
gb|EFH56558.1| cp12 domain-containing protein 1 [Arabidopsis lyrata subsp. lyrata]
Length=124
Score = 129 bits (323), Expect = 1e-35
Identities = 61/72 (85%), Positives = 66/72 (92%), Gaps = 2/72 (3%)
Frame = +1
Query 10 ISGKVEESIKNAKETCSDDPVSGECAAAWDEVEELSAAASHARDKQK--GSDPLENYCND 183
IS KVE+SI+ AKETC+DDPVSGEC AAWDEVEELSAAASHARDK+K GSDPLE YC+D
Sbjct 53 ISDKVEKSIQEAKETCADDPVSGECVAAWDEVEELSAAASHARDKKKAGGSDPLEEYCSD 112
Query 184 NPETDECRTYDN 219
NPETDECRTYDN
Sbjct 113 NPETDECRTYDN 124
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 3906626821
Number of extensions: 84147301
Number of successful extensions: 272024
Number of sequences better than 1e-10: 109
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 271529
Number of HSP's successfully gapped: 109
Length of query: 408
Length of database: 6150218869
Length adjustment: 101
Effective length of query: 307
Effective length of database: 4340391385
Effective search space: 151913698475
Effective search space used: 151913698475
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDPADPS01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_13325
Length=400
Score E
Sequences producing significant alignments: (Bits) Value
emb|CAN75757.1| hypothetical protein VITISV_028561 [Vitis vin... 72.8 2e-13
emb|CBI31637.3| unnamed protein product [Vitis vinifera] 72.8 2e-13
ref|XP_002277930.1| PREDICTED: putative DNA-binding protein E... 72.8 3e-13
ref|XP_003538420.1| PREDICTED: putative DNA-binding protein E... 65.9 7e-11
ALIGNMENTS
>emb|CAN75757.1| hypothetical protein VITISV_028561 [Vitis vinifera]
Length=293
Score = 72.8 bits (177), Expect = 2e-13
Identities = 46/101 (46%), Positives = 56/101 (55%), Gaps = 25/101 (25%)
Frame = -1
Query 394 VAGELKADGPVMVIAATFSNAVYERLPLVDEVAVGGDQGVQLP----------------- 266
V G L A GPV+VIAATFSNA +ERLPL DE A ++G+Q+P
Sbjct 198 VVGALMASGPVIVIAATFSNATFERLPLEDEPA---NEGIQMPQTSGVNSGTGGTSAPQS 254
Query 265 ---VDESKPNLYNNQQVNLIASGHMGNDVFWGPQPCPTPPY 152
VD S +Y N NL+ +G M +DVFW P P P PPY
Sbjct 255 HGLVDPSSMPIY-NJPPNLLPNGQMPHDVFWAPPPRP-PPY 293
>emb|CBI31637.3| unnamed protein product [Vitis vinifera]
Length=300
Score = 72.8 bits (177), Expect = 2e-13
Identities = 46/101 (46%), Positives = 56/101 (55%), Gaps = 25/101 (25%)
Frame = -1
Query 394 VAGELKADGPVMVIAATFSNAVYERLPLVDEVAVGGDQGVQLP----------------- 266
V G L A GPV+VIAATFSNA +ERLPL DE A ++G+Q+P
Sbjct 205 VVGALMASGPVIVIAATFSNATFERLPLEDEPA---NEGIQMPQTSGVNSGTGGTSAPQS 261
Query 265 ---VDESKPNLYNNQQVNLIASGHMGNDVFWGPQPCPTPPY 152
VD S +Y N NL+ +G M +DVFW P P P PPY
Sbjct 262 HGLVDPSSMPIY-NLPPNLLPNGQMPHDVFWAPPPRP-PPY 300
>ref|XP_002277930.1| PREDICTED: putative DNA-binding protein ESCAROLA-like [Vitis
vinifera]
Length=327
Score = 72.8 bits (177), Expect = 3e-13
Identities = 46/101 (46%), Positives = 56/101 (55%), Gaps = 25/101 (25%)
Frame = -1
Query 394 VAGELKADGPVMVIAATFSNAVYERLPLVDEVAVGGDQGVQLP----------------- 266
V G L A GPV+VIAATFSNA +ERLPL DE A ++G+Q+P
Sbjct 232 VVGALMASGPVIVIAATFSNATFERLPLEDEPA---NEGIQMPQTSGVNSGTGGTSAPQS 288
Query 265 ---VDESKPNLYNNQQVNLIASGHMGNDVFWGPQPCPTPPY 152
VD S +Y N NL+ +G M +DVFW P P P PPY
Sbjct 289 HGLVDPSSMPIY-NLPPNLLPNGQMPHDVFWAPPPRP-PPY 327
>ref|XP_003538420.1| PREDICTED: putative DNA-binding protein ESCAROLA-like [Glycine
max]
Length=289
Score = 65.9 bits (159), Expect = 7e-11
Identities = 40/95 (42%), Positives = 54/95 (57%), Gaps = 17/95 (18%)
Frame = -1
Query 394 VAGELKADGPVMVIAATFSNAVYERLPLVDEVAVGGDQGVQLPVDESKPNLYNNQQV--- 224
VAG L A GPVMVIAATF+NA YERLPL DE G++G+Q+ + + +Q +
Sbjct 198 VAGSLVASGPVMVIAATFANATYERLPLEDE---QGEEGMQVQQQQQQQQQQQSQGLGEQ 254
Query 223 ----------NLIASG-HMGNDVFWGPQPCPTPPY 152
NL+ +G +M +DV WG P P P +
Sbjct 255 VSMPMYNLPPNLLHNGQNMPHDVLWGAPPRPPPSF 289
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 212054739
Number of extensions: 4582971
Number of successful extensions: 9510
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 9500
Number of HSP's successfully gapped: 0
Length of query: 400
Length of database: 6150218869
Length adjustment: 98
Effective length of query: 302
Effective length of database: 4394148637
Effective search space: 153795202295
Effective search space used: 153795202295
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDPTTVJ016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_14096
Length=384
Score E
Sequences producing significant alignments: (Bits) Value
emb|CAB56231.1| betanidin-5-O-glucosyltransferase [Dorotheant... 55.8 8e-11
ALIGNMENTS
>emb|CAB56231.1| betanidin-5-O-glucosyltransferase [Dorotheanthus bellidiformis]
Length=489
Score = 55.8 bits (133), Expect(2) = 8e-11
Identities = 28/47 (60%), Positives = 34/47 (72%), Gaps = 0/47 (0%)
Frame = +3
Query 15 KSLIKGEDIAKAVNEVMVGEESVEMRSRVKVLSDEAKKAVEKGGSSY 155
+ +IK EDI KAV EVMVGEE E R R K L + A +A+E+GGSSY
Sbjct 424 EDVIKAEDIEKAVREVMVGEEGEERRRRAKKLKEMAWRAIEEGGSSY 470
Score = 35.0 bits (79), Expect(2) = 8e-11
Identities = 15/28 (54%), Positives = 20/28 (71%), Gaps = 0/28 (0%)
Frame = +2
Query 161 AVEKGGSSYEDLDSLFEELRSIKASNEE 244
A+E+GGSSY DL +L EEL+ S +E
Sbjct 462 AIEEGGSSYSDLSALIEELKGYHTSEKE 489
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 3561089461
Number of extensions: 67826426
Number of successful extensions: 188061
Number of sequences better than 1e-10: 1
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 187940
Number of HSP's successfully gapped: 2
Length of query: 384
Length of database: 6150218869
Length adjustment: 94
Effective length of query: 290
Effective length of database: 4465824973
Effective search space: 151838049082
Effective search space used: 151838049082
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDTF624013
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_14303
Length=380
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002272542.2| PREDICTED: GDSL esterase/lipase At3g62280... 71.6 7e-18
emb|CAN71136.1| hypothetical protein VITISV_025409 [Vitis vin... 71.6 7e-18
emb|CBI39639.3| unnamed protein product [Vitis vinifera] 69.7 1e-17
ref|XP_002277053.2| PREDICTED: GDSL esterase/lipase At1g09390... 69.7 1e-17
ref|XP_002301534.1| predicted protein [Populus trichocarpa] >... 84.7 1e-17
ALIGNMENTS
>ref|XP_002272542.2| PREDICTED: GDSL esterase/lipase At3g62280-like [Vitis vinifera]
emb|CBI37002.3| unnamed protein product [Vitis vinifera]
Length=368
Score = 71.6 bits (174), Expect(2) = 7e-18
Identities = 39/90 (43%), Positives = 56/90 (62%), Gaps = 2/90 (2%)
Frame = +1
Query 1 IDIGLNDITYG-YAS-LTLDQLIKKIPLFISGIRNAMQEIYELGGRKFVVFSVEPVGCLP 174
IDIG ND+ YAS LT +++KIP F++ I+ A+Q +Y+ G RKF + + P+GC P
Sbjct 162 IDIGQNDLLLALYASNLTYPPVLEKIPSFLAEIKLAIQNLYQFGARKFWIHNTGPLGCAP 221
Query 175 GEIAAKRKTKEILDQNKCIKSLNDAAKAFN 264
E+A T LD+ C++ N AKAFN
Sbjct 222 KELALHPHTNSDLDRIGCLEVHNKVAKAFN 251
Score = 43.1 bits (100), Expect(2) = 7e-18
Identities = 18/33 (55%), Positives = 24/33 (73%), Gaps = 0/33 (0%)
Frame = +3
Query 282 NDAAKAFNDKLKALCAELQLQLKNTTIVYVDVY 380
N AKAFN L+ +C E++L K+ TIVYVD+Y
Sbjct 244 NKVAKAFNKGLRVICEEMRLMYKDATIVYVDIY 276
>emb|CAN71136.1| hypothetical protein VITISV_025409 [Vitis vinifera]
Length=362
Score = 71.6 bits (174), Expect(2) = 7e-18
Identities = 39/90 (43%), Positives = 56/90 (62%), Gaps = 2/90 (2%)
Frame = +1
Query 1 IDIGLNDITYG-YAS-LTLDQLIKKIPLFISGIRNAMQEIYELGGRKFVVFSVEPVGCLP 174
IDIG ND+ YAS LT +++KIP F++ I+ A+Q +Y+ G RKF + + P+GC P
Sbjct 156 IDIGQNDLLLALYASNLTYPPVLEKIPSFLAEIKLAIQNLYQFGARKFWIHNTGPLGCAP 215
Query 175 GEIAAKRKTKEILDQNKCIKSLNDAAKAFN 264
E+A T LD+ C++ N AKAFN
Sbjct 216 KELALHPHTNSDLDRIGCLEVHNKVAKAFN 245
Score = 43.1 bits (100), Expect(2) = 7e-18
Identities = 18/33 (55%), Positives = 24/33 (73%), Gaps = 0/33 (0%)
Frame = +3
Query 282 NDAAKAFNDKLKALCAELQLQLKNTTIVYVDVY 380
N AKAFN L+ +C E++L K+ TIVYVD+Y
Sbjct 238 NKVAKAFNKGLRVICEEMRLMYKDATIVYVDIY 270
>emb|CBI39639.3| unnamed protein product [Vitis vinifera]
Length=433
Score = 69.7 bits (169), Expect(2) = 1e-17
Identities = 33/88 (38%), Positives = 56/88 (64%), Gaps = 1/88 (1%)
Frame = +1
Query 4 DIGLNDITYGYA-SLTLDQLIKKIPLFISGIRNAMQEIYELGGRKFVVFSVEPVGCLPGE 180
DIG ND+ ++ +L+ Q++K+IP ++ I+ A+Q +Y+ GGRKF + + P+GCLP +
Sbjct 231 DIGQNDLADSFSKNLSYAQVVKRIPFILAEIKYAIQTMYDQGGRKFWIHNTGPLGCLPQK 290
Query 181 IAAKRKTKEILDQNKCIKSLNDAAKAFN 264
++ K LD C+ + ND A+ FN
Sbjct 291 LSLVPKKPGDLDPYGCLSAYNDVARLFN 318
Score = 44.3 bits (103), Expect(2) = 1e-17
Identities = 18/33 (55%), Positives = 27/33 (82%), Gaps = 0/33 (0%)
Frame = +3
Query 282 NDAAKAFNDKLKALCAELQLQLKNTTIVYVDVY 380
ND A+ FN+ L+ LC E++ QLK++TIVYVD++
Sbjct 311 NDVARLFNEGLRHLCQEMRSQLKDSTIVYVDIF 343
>ref|XP_002277053.2| PREDICTED: GDSL esterase/lipase At1g09390-like [Vitis vinifera]
Length=375
Score = 69.7 bits (169), Expect(2) = 1e-17
Identities = 33/88 (38%), Positives = 56/88 (64%), Gaps = 1/88 (1%)
Frame = +1
Query 4 DIGLNDITYGYA-SLTLDQLIKKIPLFISGIRNAMQEIYELGGRKFVVFSVEPVGCLPGE 180
DIG ND+ ++ +L+ Q++K+IP ++ I+ A+Q +Y+ GGRKF + + P+GCLP +
Sbjct 173 DIGQNDLADSFSKNLSYAQVVKRIPFILAEIKYAIQTMYDQGGRKFWIHNTGPLGCLPQK 232
Query 181 IAAKRKTKEILDQNKCIKSLNDAAKAFN 264
++ K LD C+ + ND A+ FN
Sbjct 233 LSLVPKKPGDLDPYGCLSAYNDVARLFN 260
Score = 44.3 bits (103), Expect(2) = 1e-17
Identities = 18/33 (55%), Positives = 27/33 (82%), Gaps = 0/33 (0%)
Frame = +3
Query 282 NDAAKAFNDKLKALCAELQLQLKNTTIVYVDVY 380
ND A+ FN+ L+ LC E++ QLK++TIVYVD++
Sbjct 253 NDVARLFNEGLRHLCQEMRSQLKDSTIVYVDIF 285
>ref|XP_002301534.1| predicted protein [Populus trichocarpa]
gb|EEE80807.1| predicted protein [Populus trichocarpa]
Length=350
Score = 84.7 bits (208), Expect = 1e-17
Identities = 44/107 (41%), Positives = 63/107 (59%), Gaps = 0/107 (0%)
Frame = +1
Query 1 IDIGLNDITYGYASLTLDQLIKKIPLFISGIRNAMQEIYELGGRKFVVFSVEPVGCLPGE 180
IDIG ND+ + L+ +Q+IK I +I I AMQ IY+ GGR F + + P+GCLP +
Sbjct 148 IDIGQNDLAGSFEYLSYEQVIKNISSYIKEINYAMQNIYQHGGRNFWIHNTGPLGCLPQK 207
Query 181 IAAKRKTKEILDQNKCIKSLNDAAKAFNGKLXL*MTLQKHSMTNSKL 321
+A K DQ C+K+LNDAAK FN +L + + + NS +
Sbjct 208 LATFDKKSSDFDQYGCLKALNDAAKQFNDQLRVLCEELRSELKNSTI 254
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 4036186202
Number of extensions: 83942920
Number of successful extensions: 206611
Number of sequences better than 1e-10: 80
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 206249
Number of HSP's successfully gapped: 99
Length of query: 380
Length of database: 6150218869
Length adjustment: 92
Effective length of query: 288
Effective length of database: 4501663141
Effective search space: 153056546794
Effective search space used: 153056546794
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDTR7HZ01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_14437
Length=378
Score E
Sequences producing significant alignments: (Bits) Value
gb|ADB85100.1| proteinase inhibitor [Jatropha curcas] 106 1e-27
ref|XP_002521006.1| Proteinase inhibitor, putative [Ricinus c... 106 2e-27
ref|XP_002521007.1| Proteinase inhibitor, putative [Ricinus c... 99.4 8e-25
ref|NP_030435.1| serine protease inhibitor, potato inhibitor ... 94.7 5e-23
ref|XP_002881621.1| hypothetical protein ARALYDRAFT_482903 [A... 92.4 4e-22
ALIGNMENTS
>gb|ADB85100.1| proteinase inhibitor [Jatropha curcas]
Length=70
Score = 106 bits (265), Expect = 1e-27
Identities = 46/65 (71%), Positives = 57/65 (88%), Gaps = 0/65 (0%)
Frame = -1
Query 378 ADCPGKSSWPELVGTNGNQAAAVIERENVNVNAIVIRDGTPVPRDFRCDRVWVWIDENQV 199
++CPGKSSWPEL+G NG AAA+IE+EN +VNAIV+++GTPV RDFRCDRVWVW++E V
Sbjct 3 SECPGKSSWPELLGANGKAAAALIEKENRHVNAIVLKEGTPVTRDFRCDRVWVWVNECGV 62
Query 198 VVRTP 184
VVR P
Sbjct 63 VVRVP 67
>ref|XP_002521006.1| Proteinase inhibitor, putative [Ricinus communis]
gb|EEF41423.1| Proteinase inhibitor, putative [Ricinus communis]
Length=69
Score = 106 bits (264), Expect = 2e-27
Identities = 46/65 (71%), Positives = 56/65 (86%), Gaps = 0/65 (0%)
Frame = -1
Query 378 ADCPGKSSWPELVGTNGNQAAAVIERENVNVNAIVIRDGTPVPRDFRCDRVWVWIDENQV 199
ADC GK+SWPELVG NG++AAA IE+EN V+AIV+++GTPV +DFRC RVWVW+DEN V
Sbjct 2 ADCLGKNSWPELVGANGDEAAAAIEKENKYVDAIVLKEGTPVTKDFRCSRVWVWVDENGV 61
Query 198 VVRTP 184
V RTP
Sbjct 62 VTRTP 66
>ref|XP_002521007.1| Proteinase inhibitor, putative [Ricinus communis]
gb|EEF41424.1| Proteinase inhibitor, putative [Ricinus communis]
Length=70
Score = 99.4 bits (246), Expect = 8e-25
Identities = 42/63 (67%), Positives = 52/63 (83%), Gaps = 0/63 (0%)
Frame = -1
Query 372 CPGKSSWPELVGTNGNQAAAVIERENVNVNAIVIRDGTPVPRDFRCDRVWVWIDENQVVV 193
C GK SWPELVG G+ AAA +E+EN +V+AIV+++GTPV RDFRC+RVWVW+DEN VV
Sbjct 5 CLGKDSWPELVGAKGDDAAATVEKENKHVHAIVLKEGTPVTRDFRCNRVWVWVDENGVVT 64
Query 192 RTP 184
R P
Sbjct 65 RAP 67
>ref|NP_030435.1| serine protease inhibitor, potato inhibitor I-type protein [Arabidopsis
thaliana]
gb|AAC79626.2| putative protease inhibitor [Arabidopsis thaliana]
gb|AAM61318.1| putative protease inhibitor [Arabidopsis thaliana]
gb|AAO41943.1| putative protease inhibitor [Arabidopsis thaliana]
gb|AAO50715.1| putative protease inhibitor [Arabidopsis thaliana]
gb|AEC09597.1| serine protease inhibitor, potato inhibitor I-type protein [Arabidopsis
thaliana]
Length=70
Score = 94.7 bits (234), Expect = 5e-23
Identities = 43/64 (67%), Positives = 52/64 (81%), Gaps = 0/64 (0%)
Frame = -1
Query 375 DCPGKSSWPELVGTNGNQAAAVIERENVNVNAIVIRDGTPVPRDFRCDRVWVWIDENQVV 196
+CP K+SWPEL GTNG+ AA VIEREN VNA VI DG+PV DFRCDRV V++D N++V
Sbjct 4 ECPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIV 63
Query 195 VRTP 184
V+TP
Sbjct 64 VKTP 67
>ref|XP_002881621.1| hypothetical protein ARALYDRAFT_482903 [Arabidopsis lyrata subsp.
lyrata]
gb|EFH57880.1| hypothetical protein ARALYDRAFT_482903 [Arabidopsis lyrata subsp.
lyrata]
Length=70
Score = 92.4 bits (228), Expect = 4e-22
Identities = 41/65 (63%), Positives = 54/65 (83%), Gaps = 0/65 (0%)
Frame = -1
Query 378 ADCPGKSSWPELVGTNGNQAAAVIERENVNVNAIVIRDGTPVPRDFRCDRVWVWIDENQV 199
++CP K+SWPEL GTNG+ AA VIEREN V+A+VI DG+PV DFRCDRV V++D +++
Sbjct 3 SECPRKNSWPELRGTNGDYAATVIERENPTVDAVVILDGSPVTADFRCDRVRVFVDRHRI 62
Query 198 VVRTP 184
VV+TP
Sbjct 63 VVKTP 67
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 218681407
Number of extensions: 4826258
Number of successful extensions: 8172
Number of sequences better than 1e-10: 11
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 8159
Number of HSP's successfully gapped: 11
Length of query: 378
Length of database: 6150218869
Length adjustment: 92
Effective length of query: 286
Effective length of database: 4501663141
Effective search space: 153056546794
Effective search space used: 153056546794
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDTS7N201N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_14660
Length=372
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002526362.1| conserved hypothetical protein [Ricinus c... 77.4 8e-16
ref|XP_003632128.1| PREDICTED: uncharacterized protein LOC100... 77.4 1e-15
ref|XP_002526347.1| conserved hypothetical protein [Ricinus c... 77.4 1e-15
emb|CBI15878.3| unnamed protein product [Vitis vinifera] 77.4 1e-15
ref|XP_003632127.1| PREDICTED: uncharacterized protein LOC100... 77.4 1e-15
ALIGNMENTS
>ref|XP_002526362.1| conserved hypothetical protein [Ricinus communis]
gb|EEF36033.1| conserved hypothetical protein [Ricinus communis]
Length=135
Score = 77.4 bits (189), Expect = 8e-16
Identities = 36/50 (72%), Positives = 40/50 (80%), Gaps = 0/50 (0%)
Frame = +3
Query 57 IERAFREDERRRTAPLPEDNAVRVMEAMRRISFGGGTPDWAARVPEDQWI 206
I RAFREDE RR APL ++NA RV+EAMR ISF TPDWA RVP+DQWI
Sbjct 65 ILRAFREDENRRNAPLSQENATRVIEAMRGISFSDYTPDWAGRVPQDQWI 114
>ref|XP_003632128.1| PREDICTED: uncharacterized protein LOC100251809 isoform 2 [Vitis
vinifera]
Length=159
Score = 77.4 bits (189), Expect = 1e-15
Identities = 35/50 (70%), Positives = 40/50 (80%), Gaps = 0/50 (0%)
Frame = +3
Query 57 IERAFREDERRRTAPLPEDNAVRVMEAMRRISFGGGTPDWAARVPEDQWI 206
I RAFREDE RR APL +NA++VMEAMR +SFGG PDWA VPED+WI
Sbjct 92 IRRAFREDESRRNAPLTPENAMQVMEAMRGVSFGGLAPDWAGHVPEDRWI 141
>ref|XP_002526347.1| conserved hypothetical protein [Ricinus communis]
gb|EEF36018.1| conserved hypothetical protein [Ricinus communis]
Length=164
Score = 77.4 bits (189), Expect = 1e-15
Identities = 36/50 (72%), Positives = 40/50 (80%), Gaps = 0/50 (0%)
Frame = +3
Query 57 IERAFREDERRRTAPLPEDNAVRVMEAMRRISFGGGTPDWAARVPEDQWI 206
I RAFREDE RR APL ++NA RV+EAMR ISF TPDWA RVP+DQWI
Sbjct 94 ILRAFREDENRRNAPLSQENATRVIEAMRGISFSDYTPDWAGRVPQDQWI 143
>emb|CBI15878.3| unnamed protein product [Vitis vinifera]
Length=167
Score = 77.4 bits (189), Expect = 1e-15
Identities = 35/50 (70%), Positives = 40/50 (80%), Gaps = 0/50 (0%)
Frame = +3
Query 57 IERAFREDERRRTAPLPEDNAVRVMEAMRRISFGGGTPDWAARVPEDQWI 206
I RAFREDE RR APL +NA++VMEAMR +SFGG PDWA VPED+WI
Sbjct 100 IRRAFREDESRRNAPLTPENAMQVMEAMRGVSFGGLAPDWAGHVPEDRWI 149
>ref|XP_003632127.1| PREDICTED: uncharacterized protein LOC100251809 isoform 1 [Vitis
vinifera]
Length=170
Score = 77.4 bits (189), Expect = 1e-15
Identities = 35/50 (70%), Positives = 40/50 (80%), Gaps = 0/50 (0%)
Frame = +3
Query 57 IERAFREDERRRTAPLPEDNAVRVMEAMRRISFGGGTPDWAARVPEDQWI 206
I RAFREDE RR APL +NA++VMEAMR +SFGG PDWA VPED+WI
Sbjct 103 IRRAFREDESRRNAPLTPENAMQVMEAMRGVSFGGLAPDWAGHVPEDRWI 152
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 161186201
Number of extensions: 3115380
Number of successful extensions: 11746
Number of sequences better than 1e-10: 1
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 11738
Number of HSP's successfully gapped: 1
Length of query: 372
Length of database: 6150218869
Length adjustment: 90
Effective length of query: 282
Effective length of database: 4537501309
Effective search space: 154275044506
Effective search space used: 154275044506
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDTUWS801S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_14713
Length=371
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003631291.1| PREDICTED: uncharacterized WD repeat-cont... 115 3e-28
emb|CAN76269.1| hypothetical protein VITISV_004097 [Vitis vin... 115 3e-28
ref|XP_002336688.1| predicted protein [Populus trichocarpa] >... 111 7e-28
ref|XP_003613387.1| WD repeat-containing protein-like protein... 112 4e-27
ref|XP_002318017.1| predicted protein [Populus trichocarpa] >... 110 1e-26
ALIGNMENTS
>ref|XP_003631291.1| PREDICTED: uncharacterized WD repeat-containing protein alr3466-like
[Vitis vinifera]
Length=427
Score = 115 bits (287), Expect = 3e-28
Identities = 55/85 (65%), Positives = 68/85 (80%), Gaps = 3/85 (4%)
Frame = -1
Query 371 AILCLAVVNDLLFSGSADKTVRVWKRGGSGNSYFCLAVFEGHKNPVKCLTACLDSNNSSR 192
AILCLA+V+DL+FSGSADKTVR+WK+ G SY CLAVFEGH+ PVKCLTA +D+ N
Sbjct 340 AILCLAIVSDLVFSGSADKTVRIWKK-GLQRSYSCLAVFEGHRGPVKCLTAAVDNYNGRS 398
Query 191 GGGNECGNAYVVYSGSLDCEIKVWK 117
++ AY++YSGSLDCEIKVW+
Sbjct 399 NSDDDA--AYLIYSGSLDCEIKVWQ 421
>emb|CAN76269.1| hypothetical protein VITISV_004097 [Vitis vinifera]
Length=507
Score = 115 bits (288), Expect = 3e-28
Identities = 56/85 (66%), Positives = 68/85 (80%), Gaps = 3/85 (4%)
Frame = -1
Query 371 AILCLAVVNDLLFSGSADKTVRVWKRGGSGNSYFCLAVFEGHKNPVKCLTACLDSNNSSR 192
AILCLAVV+DL+FSGSADKTVR+WK+ G SY CLAVFEGH+ PVKCLTA +D+ N
Sbjct 420 AILCLAVVSDLVFSGSADKTVRIWKK-GLQRSYSCLAVFEGHRGPVKCLTAAVDNYNGRS 478
Query 191 GGGNECGNAYVVYSGSLDCEIKVWK 117
++ AY++YSGSLDCEIKVW+
Sbjct 479 NSDDDA--AYLIYSGSLDCEIKVWQ 501
>ref|XP_002336688.1| predicted protein [Populus trichocarpa]
gb|EEE74935.1| predicted protein [Populus trichocarpa]
Length=240
Score = 111 bits (278), Expect = 7e-28
Identities = 54/90 (60%), Positives = 70/90 (78%), Gaps = 9/90 (10%)
Frame = -1
Query 371 AILCLAVVNDLLFSGSADKTVRVWKRGGSGNSYFCLAVFEGHKNPVKCLTACLDSNNSSR 192
+ILCLAVV+DLLFSGSADKT+R+W+ GS SY CLAV EGH+ PVKCLTA +D +N++
Sbjct 159 SILCLAVVSDLLFSGSADKTIRIWR--GSDKSYSCLAVLEGHRGPVKCLTATIDHDNTTD 216
Query 191 GGGNECGNAYVVYSGSLDCEIKVWKFWVPL 102
+Y++YSGSLDC+I+VW+ VPL
Sbjct 217 A-------SYLLYSGSLDCDIRVWQINVPL 239
>ref|XP_003613387.1| WD repeat-containing protein-like protein [Medicago truncatula]
gb|AES96345.1| WD repeat-containing protein-like protein [Medicago truncatula]
Length=448
Score = 112 bits (279), Expect = 4e-27
Identities = 52/90 (58%), Positives = 67/90 (74%), Gaps = 0/90 (0%)
Frame = -1
Query 371 AILCLAVVNDLLFSGSADKTVRVWKRGGSGNSYFCLAVFEGHKNPVKCLTACLDSNNSSR 192
AILCL V++DL+ SGSAD +VR+WKRG SY CLAV +GH+ VKCL DS +
Sbjct 358 AILCLVVMDDLVCSGSADNSVRLWKRGIDEKSYTCLAVLQGHRKAVKCLAIADDSKSGKN 417
Query 191 GGGNECGNAYVVYSGSLDCEIKVWKFWVPL 102
GG ++ G++Y+VYSGSLDC+IKVW+ VPL
Sbjct 418 GGVDDDGSSYLVYSGSLDCDIKVWQIRVPL 447
>ref|XP_002318017.1| predicted protein [Populus trichocarpa]
gb|EEE96237.1| predicted protein [Populus trichocarpa]
Length=346
Score = 110 bits (274), Expect = 1e-26
Identities = 53/89 (60%), Positives = 69/89 (78%), Gaps = 9/89 (10%)
Frame = -1
Query 371 AILCLAVVNDLLFSGSADKTVRVWKRGGSGNSYFCLAVFEGHKNPVKCLTACLDSNNSSR 192
+ILCLAVV+DLLFSGSADKT+R+W+ GS SY CLAV EGH+ PVKCLTA +D +N++
Sbjct 267 SILCLAVVSDLLFSGSADKTIRIWR--GSDKSYSCLAVLEGHRGPVKCLTATIDHDNTTD 324
Query 191 GGGNECGNAYVVYSGSLDCEIKVWKFWVP 105
+Y++YSGSLDC+I+VW+ VP
Sbjct 325 A-------SYLLYSGSLDCDIRVWQINVP 346
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 152062734
Number of extensions: 2672264
Number of successful extensions: 9387
Number of sequences better than 1e-10: 1
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 9042
Number of HSP's successfully gapped: 1
Length of query: 371
Length of database: 6150218869
Length adjustment: 89
Effective length of query: 282
Effective length of database: 4555420393
Effective search space: 154884293362
Effective search space used: 154884293362
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDUUDBD01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_14819
Length=369
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 171736385
Number of extensions: 3197082
Number of successful extensions: 6741
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 6741
Number of HSP's successfully gapped: 0
Length of query: 369
Length of database: 6150218869
Length adjustment: 89
Effective length of query: 280
Effective length of database: 4555420393
Effective search space: 154884293362
Effective search space used: 154884293362
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDUZHBU01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_15459
Length=356
Score E
Sequences producing significant alignments: (Bits) Value
gb|ACB12048.1| pathogenesis-related protein [Rehmannia glutin... 104 4e-26
gb|ABR10301.1| pathogen-related protein STH-2 [Salvia miltior... 99.4 6e-24
sp|P17641.1|PRS1_SOLTU RecName: Full=Pathogenesis-related pro... 94.4 4e-22
ref|XP_003609710.1| Pathogenesis-related protein [Medicago tr... 94.4 5e-22
sp|P17642.1|PRS2_SOLTU RecName: Full=Pathogenesis-related pro... 93.6 8e-22
ALIGNMENTS
>gb|ACB12048.1| pathogenesis-related protein [Rehmannia glutinosa]
Length=154
Score = 104 bits (260), Expect = 4e-26
Identities = 50/92 (54%), Positives = 65/92 (71%), Gaps = 0/92 (0%)
Frame = +1
Query 37 KHRIDVLDAEKGTTKFTMIEGAWSGDKIESVVFDVKFEEVSGGGCTIKIVNEYNTKGDVA 216
K RI+ +D + +K+T+IEG GDKIES+ ++ KFE+ S GGC KIV EY+TKGD+
Sbjct 63 KIRIEAVDIDNQVSKYTVIEGPMLGDKIESIHYEQKFEDSSDGGCVAKIVCEYHTKGDIQ 122
Query 217 LKDEDIKEIIDGTKGFYTAAEAYLTANPTVCA 312
LK+E +K I D GFYT +E YL ANP VCA
Sbjct 123 LKEEGVKAINDQALGFYTLSEEYLHANPNVCA 154
>gb|ABR10301.1| pathogen-related protein STH-2 [Salvia miltiorrhiza]
Length=160
Score = 99.4 bits (246), Expect = 6e-24
Identities = 46/99 (46%), Positives = 64/99 (65%), Gaps = 0/99 (0%)
Frame = +1
Query 16 GAPVPCAKHRIDVLDAEKGTTKFTMIEGAWSGDKIESVVFDVKFEEVSGGGCTIKIVNEY 195
GA K R+D +D EK + K+T+IEG GDK+E + +D+KFE+ GGC +K+ +EY
Sbjct 62 GAHFKYMKCRVDEIDHEKHSIKYTLIEGDMLGDKLEKICYDMKFEDTEDGGCVVKVTSEY 121
Query 196 NTKGDVALKDEDIKEIIDGTKGFYTAAEAYLTANPTVCA 312
+TKG L DED+K + + G Y + E YL ANP VCA
Sbjct 122 HTKGGYELADEDLKGAKEQSLGMYKSCEDYLLANPHVCA 160
>sp|P17641.1|PRS1_SOLTU RecName: Full=Pathogenesis-related protein STH-21
gb|AAA03020.1| pSTH-21 protein [Solanum tuberosum]
gb|AAA02829.1| STH-21 protein [Solanum tuberosum]
Length=155
Score = 94.4 bits (233), Expect = 4e-22
Identities = 48/101 (48%), Positives = 63/101 (62%), Gaps = 0/101 (0%)
Frame = +1
Query 10 VAGAPVPCAKHRIDVLDAEKGTTKFTMIEGAWSGDKIESVVFDVKFEEVSGGGCTIKIVN 189
V G+P+ KH+I V+D + TK++MIEG GDK+ES+ +D+KFE GGC K +
Sbjct 55 VEGSPIKYLKHKIHVVDDKNLVTKYSMIEGDVLGDKLESISYDLKFEAHGNGGCVCKSIA 114
Query 190 EYNTKGDVALKDEDIKEIIDGTKGFYTAAEAYLTANPTVCA 312
EY+TKGD LKDED E + EAYL ANP+V A
Sbjct 115 EYHTKGDYVLKDEDHNEGKKQGMELFKIVEAYLLANPSVYA 155
>ref|XP_003609710.1| Pathogenesis-related protein [Medicago truncatula]
gb|AES91907.1| Pathogenesis-related protein [Medicago truncatula]
Length=160
Score = 94.4 bits (233), Expect = 5e-22
Identities = 47/99 (47%), Positives = 61/99 (62%), Gaps = 0/99 (0%)
Frame = +1
Query 16 GAPVPCAKHRIDVLDAEKGTTKFTMIEGAWSGDKIESVVFDVKFEEVSGGGCTIKIVNEY 195
G P K +IDVLD E K+TMIEG GDK+ES+ ++VKFE + GGC K+ + Y
Sbjct 62 GNPFKYLKQKIDVLDKENLICKYTMIEGDPLGDKLESIAYEVKFEATNDGGCLCKMASSY 121
Query 196 NTKGDVALKDEDIKEIIDGTKGFYTAAEAYLTANPTVCA 312
T GD +K+ED+KE + T G Y E+YL NP V A
Sbjct 122 KTIGDFDVKEEDVKEGRESTIGIYEVVESYLLENPQVYA 160
>sp|P17642.1|PRS2_SOLTU RecName: Full=Pathogenesis-related protein STH-2
gb|AAA03019.1| pSTH-2 protein [Solanum tuberosum]
gb|AAA02828.1| STH-2 protein [Solanum tuberosum]
Length=155
Score = 93.6 bits (231), Expect = 8e-22
Identities = 47/101 (47%), Positives = 63/101 (62%), Gaps = 0/101 (0%)
Frame = +1
Query 10 VAGAPVPCAKHRIDVLDAEKGTTKFTMIEGAWSGDKIESVVFDVKFEEVSGGGCTIKIVN 189
V G+P+ KH+I V+D + TK++MIEG GDK+ES+ +D+KFE GGC K +
Sbjct 55 VEGSPIKYLKHKIHVVDDKNLVTKYSMIEGDVLGDKLESISYDLKFEAHGNGGCVCKSIT 114
Query 190 EYNTKGDVALKDEDIKEIIDGTKGFYTAAEAYLTANPTVCA 312
EY+TKGD LKDE+ E + EAYL ANP+V A
Sbjct 115 EYHTKGDYVLKDEEHNEGQKQGMELFKIVEAYLLANPSVYA 155
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 168044670
Number of extensions: 3233379
Number of successful extensions: 8281
Number of sequences better than 1e-10: 10
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 8277
Number of HSP's successfully gapped: 10
Length of query: 356
Length of database: 6150218869
Length adjustment: 85
Effective length of query: 271
Effective length of database: 4627096729
Effective search space: 152694192057
Effective search space used: 152694192057
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD0XEP801N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_1590
Length=908
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002880073.1| hypothetical protein ARALYDRAFT_903792 [A... 328 4e-107
ref|NP_181916.1| putative polygalacturonase /pectinase [Arabi... 326 2e-106
ref|XP_002331633.1| predicted protein [Populus trichocarpa] >... 320 5e-104
ref|XP_002331634.1| predicted protein [Populus trichocarpa] >... 317 4e-103
emb|CAN68140.1| hypothetical protein VITISV_035657 [Vitis vin... 314 9e-102
ALIGNMENTS
>ref|XP_002880073.1| hypothetical protein ARALYDRAFT_903792 [Arabidopsis lyrata subsp.
lyrata]
gb|EFH56332.1| hypothetical protein ARALYDRAFT_903792 [Arabidopsis lyrata subsp.
lyrata]
Length=393
Score = 328 bits (840), Expect = 4e-107
Identities = 160/261 (61%), Positives = 203/261 (78%), Gaps = 5/261 (2%)
Frame = -1
Query 908 CRRGRYNRCPGGARSLAFEWCNNVNVRGLRSFNSQiihisishsSNMKLQNVRIVAPSGS 729
CR+ + + CP GARS++F WCNNV + GL SFNSQ +H+++ HSSN+++QN+RI APSGS
Sbjct 136 CRK-KGSHCPQGARSISFSWCNNVLLSGLTSFNSQNMHVTVHHSSNVRIQNIRIRAPSGS 194
Query 728 PNTDGIHISASRGVTVTDSIIKTGDDCISIGPGTMNVWMERIGCGPGHGISIGSLGDSYN 549
PNTDGIHI +S GVT++ I TGDDCI++ G+ NVW+ER+ CGPGHGISIGSLGD N
Sbjct 195 PNTDGIHIQSSSGVTISGGTIATGDDCIALSQGSRNVWIERVNCGPGHGISIGSLGDYAN 254
Query 548 EAGVQNVTVTNSVFTKTQNGVRVKSWARASVGYARNLVFKNLVMRNVANPILIDQNYCPS 369
E GVQNVTVT+SVFTKTQNGVR+K+WAR S G+ N+VF+NL+M NV NP++IDQNYCP+
Sbjct 255 EEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVGNPVIIDQNYCPN 314
Query 368 GS-CPHQSSGVRVSQVLYRNIKGTSATQAAMTFRCSQSRPCYGIRLADIRLTYVNTLRRP 192
G+ CP QSSGV++S V + NIKGTS T AM CS S C G+RL DI+LTY +RR
Sbjct 315 GNGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSSHCTGLRLQDIKLTY---MRRS 371
Query 191 TVAYCENAGGSSRGAVFPRTC 129
+ +YC NA G + G + PR C
Sbjct 372 SASYCRNAHGRASGVMVPRNC 392
>ref|NP_181916.1| putative polygalacturonase /pectinase [Arabidopsis thaliana]
gb|AAC23426.1| putative polygalacturonase [Arabidopsis thaliana]
gb|AEC10340.1| putative polygalacturonase /pectinase [Arabidopsis thaliana]
Length=394
Score = 326 bits (835), Expect = 2e-106
Identities = 158/261 (61%), Positives = 202/261 (77%), Gaps = 5/261 (2%)
Frame = -1
Query 908 CRRGRYNRCPGGARSLAFEWCNNVNVRGLRSFNSQiihisishsSNMKLQNVRIVAPSGS 729
CR+ + + CP GARS++F WCNNV + GL SFNSQ +H+++ HSSN++++NVRI APSGS
Sbjct 137 CRK-KGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSSNVRIENVRIRAPSGS 195
Query 728 PNTDGIHISASRGVTVTDSIIKTGDDCISIGPGTMNVWMERIGCGPGHGISIGSLGDSYN 549
PNTDGIH+ +S GVT++ I TGDDCI++ G+ N+W+ER+ CGPGHGISIGSLGD N
Sbjct 196 PNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCGPGHGISIGSLGDYAN 255
Query 548 EAGVQNVTVTNSVFTKTQNGVRVKSWARASVGYARNLVFKNLVMRNVANPILIDQNYCPS 369
E GVQNVTVT+SVFTKTQNGVR+K+WAR S G+ N+VF+NL+M NV NP++IDQNYCP+
Sbjct 256 EEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVENPVIIDQNYCPN 315
Query 368 G-SCPHQSSGVRVSQVLYRNIKGTSATQAAMTFRCSQSRPCYGIRLADIRLTYVNTLRRP 192
G CP QSSGV++S V + NIKGTS T AM CS S C G+RL DI+LTY +RR
Sbjct 316 GKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLRLQDIKLTY---MRRS 372
Query 191 TVAYCENAGGSSRGAVFPRTC 129
+ +YC NA G + G + PR C
Sbjct 373 SASYCRNAHGRASGVMVPRNC 393
>ref|XP_002331633.1| predicted protein [Populus trichocarpa]
gb|EEF11160.1| predicted protein [Populus trichocarpa]
Length=390
Score = 320 bits (819), Expect = 5e-104
Identities = 154/261 (59%), Positives = 198/261 (76%), Gaps = 5/261 (2%)
Frame = -1
Query 908 CRRGRYNRCPGGARSLAFEWCNNVNVRGLRSFNSQiihisishsSNMKLQNVRIVAPSGS 729
CR+ N CP GARS+ F W N++ + GL S NSQ +HI I+ +N+ ++NVR++AP S
Sbjct 133 CRKSGQN-CPVGARSITFNWANDILISGLTSINSQSMHIVINSCNNVLVRNVRVIAPYNS 191
Query 728 PNTDGIHISASRGVTVTDSIIKTGDDCISIGPGTMNVWMERIGCGPGHGISIGSLGDSYN 549
PNTDGIH+ S GVT+T S ++TGDDCISIGP T N+ M I CGPGHGISIGSLG +N
Sbjct 192 PNTDGIHVQISTGVTITGSTLQTGDDCISIGPSTRNLLMSSIKCGPGHGISIGSLGKDFN 251
Query 548 EAGVQNVTVTNSVFTKTQNGVRVKSWARASVGYARNLVFKNLVMRNVANPILIDQNYCPS 369
E GV+N+T+TNS+F+ + NGVR+KSWAR S G+ RN+VF+NL+M+NV NPI+IDQNYCP+
Sbjct 252 EDGVENITLTNSIFSGSDNGVRIKSWARPSNGFVRNVVFQNLIMKNVKNPIIIDQNYCPN 311
Query 368 G-SCPHQSSGVRVSQVLYRNIKGTSATQAAMTFRCSQSRPCYGIRLADIRLTYVNTLRRP 192
CPHQSSGV++SQV YRNI+GTSAT A+TF CS S PC GI L DI+LTY+NT
Sbjct 312 NQGCPHQSSGVQISQVTYRNIQGTSATPKAVTFDCSPSNPCRGIELQDIKLTYLNT---A 368
Query 191 TVAYCENAGGSSRGAVFPRTC 129
+ C+N GG+S G + P +C
Sbjct 369 ATSSCKNIGGTSSGVLMPESC 389
>ref|XP_002331634.1| predicted protein [Populus trichocarpa]
gb|EEF11161.1| predicted protein [Populus trichocarpa]
Length=390
Score = 317 bits (813), Expect = 4e-103
Identities = 150/261 (57%), Positives = 198/261 (76%), Gaps = 5/261 (2%)
Frame = -1
Query 908 CRRGRYNRCPGGARSLAFEWCNNVNVRGLRSFNSQiihisishsSNMKLQNVRIVAPSGS 729
CR+ N CP GARS+ F W N++ + GL S NSQ +H+ I+ +N+ ++NVR++AP S
Sbjct 133 CRKSGQN-CPVGARSITFNWANDILISGLTSINSQSMHLVINSCNNVLVRNVRVIAPDQS 191
Query 728 PNTDGIHISASRGVTVTDSIIKTGDDCISIGPGTMNVWMERIGCGPGHGISIGSLGDSYN 549
PNTDGIH+ S GVT+T S ++TGDDCISIGP T N+ M I CGPGHGISIGSLG +N
Sbjct 192 PNTDGIHVQTSTGVTITGSTLQTGDDCISIGPSTRNMLMSSIKCGPGHGISIGSLGKDFN 251
Query 548 EAGVQNVTVTNSVFTKTQNGVRVKSWARASVGYARNLVFKNLVMRNVANPILIDQNYCPS 369
E GV+N+T+TNS+F+ + NGVR+KSWAR S G+ RN+VF+NL+M+NV NPI++DQNYCP+
Sbjct 252 EGGVENITLTNSIFSGSDNGVRIKSWARPSNGFVRNVVFQNLIMKNVRNPIIVDQNYCPN 311
Query 368 G-SCPHQSSGVRVSQVLYRNIKGTSATQAAMTFRCSQSRPCYGIRLADIRLTYVNTLRRP 192
CP QSSGV++SQV YRNI+GTSA+ A+TF CS S PC GI+L DI+LTY+NT
Sbjct 312 NQGCPRQSSGVKISQVTYRNIQGTSASPEAVTFDCSSSNPCRGIKLQDIKLTYMNT---A 368
Query 191 TVAYCENAGGSSRGAVFPRTC 129
+ C+N GG+S G + P +C
Sbjct 369 ATSSCKNIGGTSSGVLMPESC 389
>emb|CAN68140.1| hypothetical protein VITISV_035657 [Vitis vinifera]
emb|CBI24748.3| unnamed protein product [Vitis vinifera]
Length=389
Score = 314 bits (804), Expect = 9e-102
Identities = 157/261 (60%), Positives = 189/261 (72%), Gaps = 5/261 (2%)
Frame = -1
Query 908 CRRGRYNRCPGGARSLAFEWCNNVNVRGLRSFNSQiihisishsSNMKLQNVRIVAPSGS 729
CRR + CP GARS+ F W NN+ V GL S NSQ+ H+ I+ N+ ++NV+I+AP S
Sbjct 132 CRRSGKS-CPIGARSITFNWANNIVVSGLTSINSQLSHLVINSCKNVAVRNVKIIAPDQS 190
Query 728 PNTDGIHISASRGVTVTDSIIKTGDDCISIGPGTMNVWMERIGCGPGHGISIGSLGDSYN 549
PNTDGIH+ S GVT+T S I TGDDCISIGPGT N+WME I CGPGHGISIGSLG
Sbjct 191 PNTDGIHVQGSTGVTITGSTIGTGDDCISIGPGTRNLWMEHIKCGPGHGISIGSLGKDKK 250
Query 548 EAGVQNVTVTNSVFTKTQNGVRVKSWARASVGYARNLVFKNLVMRNVANPILIDQNYCPS 369
E GVQNVT+TNSVF + NGVR+KSWAR S + N+VF+N+VM V PI++DQNYCP+
Sbjct 251 EDGVQNVTLTNSVFIGSDNGVRIKSWARPSNSFVTNIVFRNIVMTRVQYPIIVDQNYCPN 310
Query 368 G-SCPHQSSGVRVSQVLYRNIKGTSATQAAMTFRCSQSRPCYGIRLADIRLTYVNTLRRP 192
CP+QSSGV+VSQV YRNIKGTS +QAAM F CS S PC GIRL DI LTY+N +
Sbjct 311 NQGCPNQSSGVKVSQVTYRNIKGTSRSQAAMIFNCSSSNPCRGIRLQDINLTYMN---KA 367
Query 191 TVAYCENAGGSSRGAVFPRTC 129
+ C N G+ G V PR+C
Sbjct 368 ATSTCRNVHGTRSGVVVPRSC 388
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 483056143
Number of extensions: 10563898
Number of successful extensions: 24732
Number of sequences better than 1e-10: 67
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 24568
Number of HSP's successfully gapped: 67
Length of query: 908
Length of database: 6150218869
Length adjustment: 140
Effective length of query: 768
Effective length of database: 3641547109
Effective search space: 589930631658
Effective search space used: 589930631658
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 176 (72.4 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDV2RZM01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_16555
Length=337
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003560817.1| PREDICTED: chemocyanin-like [Brachypodium... 70.9 1e-13
ref|NP_001237170.1| uncharacterized protein LOC100499869 prec... 69.7 3e-13
ref|XP_002298184.1| predicted protein [Populus trichocarpa] >... 69.7 4e-13
ref|NP_001235375.1| uncharacterized protein LOC100305948 prec... 68.6 9e-13
emb|CAA10134.1| basic blue copper protein [Cicer arietinum] 66.6 5e-12
ALIGNMENTS
>ref|XP_003560817.1| PREDICTED: chemocyanin-like [Brachypodium distachyon]
Length=130
Score = 70.9 bits (172), Expect = 1e-13
Identities = 43/107 (40%), Positives = 58/107 (54%), Gaps = 10/107 (9%)
Frame = +1
Query 19 AAAMVSIMLQLVLHFDEAKAKNYVVGNTYNAPAFYGWTDVSPSHIYYPSGLTLRTGDTLQ 198
AA + ++L ++LH + A++ Y VG+ GWT S +P G R GD LQ
Sbjct 15 AAVLALVLLCVLLHGEFAESAVYTVGDRG------GWTLNSGG---WPRGKRFRAGDVLQ 65
Query 199 FTYYNKFHNVVGVGLNGYKNCDSNRGG-FFRSGNDTIPLKPGWNYFI 336
F Y HNVV V GYK+C + RG + SGND++ L G NYFI
Sbjct 66 FKYGRGAHNVVAVNAAGYKSCSAPRGAKVYSSGNDSVKLSRGTNYFI 112
>ref|NP_001237170.1| uncharacterized protein LOC100499869 precursor [Glycine max]
gb|ACU13981.1| unknown [Glycine max]
Length=121
Score = 69.7 bits (169), Expect = 3e-13
Identities = 44/111 (40%), Positives = 59/111 (53%), Gaps = 10/111 (9%)
Frame = +1
Query 7 ALVVAAAMVSIMLQLVLHFDEAKAKNYVVGNTYNAPAFYGWTDVSPSHIYYPSGLTLRTG 186
AL +A+V ++ L+LH A+A Y VG++ GWT + + +P G R G
Sbjct 2 ALGRGSAVVLLLCFLLLHSQMARAATYTVGDSG------GWTF---NTVAWPKGKLFRAG 52
Query 187 DTLQFTYYNKFHNVVGVGLNGYKNCDSNRGG-FFRSGNDTIPLKPGWNYFI 336
DTL F Y HNVV V GY +C + RG ++SG D I L G NYFI
Sbjct 53 DTLAFNYSPGTHNVVAVNKAGYDSCKTPRGAKVYKSGTDQIRLAKGQNYFI 103
>ref|XP_002298184.1| predicted protein [Populus trichocarpa]
gb|EEE82989.1| predicted protein [Populus trichocarpa]
Length=125
Score = 69.7 bits (169), Expect = 4e-13
Identities = 47/114 (41%), Positives = 59/114 (52%), Gaps = 13/114 (11%)
Frame = +1
Query 1 RGALVVAAAMVSIMLQLVLHFDEAKAKNYVVGNTYNAPAFYGWT-DVSPSHIYYPSGLTL 177
RG+ +VA V + + L+LHFD A A Y VG GWT +VS +P G +
Sbjct 5 RGSAMVATVAVMLCM-LLLHFDMAHAATYTVGGPG------GWTFNVSG----WPKGKSF 53
Query 178 RTGDTLQFTYYNKFHNVVGVGLNGYKNCDSNRGG-FFRSGNDTIPLKPGWNYFI 336
+ GD L F Y HNVV V GY +C S RG + SG D I L G N+FI
Sbjct 54 KAGDILVFNYSTAAHNVVAVNKAGYSSCTSPRGAKVYTSGKDQIKLVKGQNFFI 107
>ref|NP_001235375.1| uncharacterized protein LOC100305948 precursor [Glycine max]
gb|ACU13882.1| unknown [Glycine max]
Length=121
Score = 68.6 bits (166), Expect = 9e-13
Identities = 45/111 (41%), Positives = 59/111 (53%), Gaps = 10/111 (9%)
Frame = +1
Query 7 ALVVAAAMVSIMLQLVLHFDEAKAKNYVVGNTYNAPAFYGWTDVSPSHIYYPSGLTLRTG 186
AL +A+V ++ LVL + A+A Y VG++ GWT + + +P G R G
Sbjct 2 ALGRGSAVVLLLCFLVLQSEMARAATYRVGDSR------GWTF---NTVTWPQGKRFRAG 52
Query 187 DTLQFTYYNKFHNVVGVGLNGYKNCDSNRGG-FFRSGNDTIPLKPGWNYFI 336
DTL F Y HNVV V GY +C + RG +RSG D I L G NYFI
Sbjct 53 DTLAFNYSPGAHNVVAVSKAGYDSCKTPRGAKVYRSGKDQIRLARGQNYFI 103
>emb|CAA10134.1| basic blue copper protein [Cicer arietinum]
Length=122
Score = 66.6 bits (161), Expect = 5e-12
Identities = 43/107 (40%), Positives = 57/107 (53%), Gaps = 10/107 (9%)
Frame = +1
Query 19 AAAMVSIMLQLVLHFDEAKAKNYVVGNTYNAPAFYGWTDVSPSHIYYPSGLTLRTGDTLQ 198
+A +V ++ LV+H + A+A Y VG GWT + I +P+G + GDTL
Sbjct 7 SALVVLLVCFLVIHSELAQAAIYTVGGAG------GWTF---NTIAWPNGKNFKAGDTLV 57
Query 199 FTYYNKFHNVVGVGLNGYKNCDSNRGG-FFRSGNDTIPLKPGWNYFI 336
F Y HNVV V GY +C + RG +RSG D I L G NYFI
Sbjct 58 FNYSPGAHNVVAVSKAGYGSCKTPRGAKVYRSGKDQIRLARGQNYFI 104
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 191802069
Number of extensions: 4302931
Number of successful extensions: 10016
Number of sequences better than 1e-10: 1
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 9991
Number of HSP's successfully gapped: 1
Length of query: 337
Length of database: 6150218869
Length adjustment: 80
Effective length of query: 257
Effective length of database: 4716692149
Effective search space: 150934148768
Effective search space used: 150934148768
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD1V0E601S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_1803
Length=883
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002522043.1| phosphate abc transporter, putative [Rici... 369 6e-125
ref|XP_002325227.1| predicted protein [Populus trichocarpa] >... 367 1e-124
ref|XP_002266816.1| PREDICTED: ABC transporter I family membe... 360 1e-121
emb|CAN70271.1| hypothetical protein VITISV_010974 [Vitis vin... 359 1e-121
ref|XP_003530971.1| PREDICTED: ABC transporter I family membe... 352 2e-118
ALIGNMENTS
>ref|XP_002522043.1| phosphate abc transporter, putative [Ricinus communis]
gb|EEF40243.1| phosphate abc transporter, putative [Ricinus communis]
Length=276
Score = 369 bits (946), Expect = 6e-125
Identities = 181/236 (77%), Positives = 215/236 (91%), Gaps = 0/236 (0%)
Frame = -1
Query 865 KIEVRGLTKVSDKDVRILNKVNLDIQRGVIMGVIGPSGSGKSTFLRALNRLWEPASGSVF 686
K +R LT+ +D +ILN VNLD+ +GVI+G++GPSGSGKSTFLR+LNRLWEP G+VF
Sbjct 41 KFRIRNLTRETDGGAKILNGVNLDVPKGVIVGIVGPSGSGKSTFLRSLNRLWEPPPGTVF 100
Query 685 LDGLDICELDVLNLRRKVGMLFQLPALFEGTVADNVRYGPQLRGQKLSDVDVHTLLALAD 506
LDG DI +LDVL+LRRKVGMLFQ+P LFEGT+ADN+RYGPQLRG+KLSD +VH LL LAD
Sbjct 101 LDGCDIRDLDVLSLRRKVGMLFQIPVLFEGTIADNIRYGPQLRGKKLSDNEVHKLLILAD 160
Query 505 LDPSFYNKSGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVLLKLKK 326
LD SF+ K+ GELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIE+V++KLKK
Sbjct 161 LDSSFHKKNYGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEEVIVKLKK 220
Query 325 DRGMTIVMVSHSIKQIQRIADVVCLVVDGEIVEILEPNKLSEAKHPMAKRFLELGS 158
++GMTIVMVSHSIKQIQR+ADVVCL+V+GE+VE+L+PN+LSEAKHPMA+RFL+L S
Sbjct 221 NQGMTIVMVSHSIKQIQRVADVVCLLVNGEVVEVLKPNELSEAKHPMAQRFLQLSS 276
>ref|XP_002325227.1| predicted protein [Populus trichocarpa]
gb|EEF03792.1| predicted protein [Populus trichocarpa]
Length=272
Score = 367 bits (943), Expect = 1e-124
Identities = 184/237 (78%), Positives = 215/237 (91%), Gaps = 0/237 (0%)
Frame = -1
Query 868 AKIEVRGLTKVSDKDVRILNKVNLDIQRGVIMGVIGPSGSGKSTFLRALNRLWEPASGSV 689
AK +R LTKVSD V ILN +N+DI +G+I+G+IGPSGSGKST LRALNRLWEP SG+V
Sbjct 36 AKFRIRNLTKVSDAGVPILNGINMDIPKGMIVGIIGPSGSGKSTVLRALNRLWEPPSGTV 95
Query 688 FLDGLDICELDVLNLRRKVGMLFQLPALFEGTVADNVRYGPQLRGQKLSDVDVHTLLALA 509
FLDG D+ +LDVL RRKVGMLFQLPALF+GTVADN+RYGPQL+G+KL+D +V+ LL+LA
Sbjct 96 FLDGHDVKDLDVLGHRRKVGMLFQLPALFDGTVADNIRYGPQLKGKKLTDDEVYKLLSLA 155
Query 508 DLDPSFYNKSGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVLLKLK 329
DLD SFY K+ ELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVL+KLK
Sbjct 156 DLDSSFYKKTSNELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVLVKLK 215
Query 328 KDRGMTIVMVSHSIKQIQRIADVVCLVVDGEIVEILEPNKLSEAKHPMAKRFLELGS 158
K +G+T+V+VSHSIKQIQRIADVVCL+V+GEIVE+L+P+ LSEAKHPMA+RFL+LGS
Sbjct 216 KQQGLTVVIVSHSIKQIQRIADVVCLLVNGEIVEVLKPDDLSEAKHPMAQRFLQLGS 272
>ref|XP_002266816.1| PREDICTED: ABC transporter I family member 17 [Vitis vinifera]
emb|CBI17869.3| unnamed protein product [Vitis vinifera]
Length=268
Score = 360 bits (924), Expect = 1e-121
Identities = 183/236 (78%), Positives = 206/236 (87%), Gaps = 0/236 (0%)
Frame = -1
Query 865 KIEVRGLTKVSDKDVRILNKVNLDIQRGVIMGVIGPSGSGKSTFLRALNRLWEPASGSVF 686
K VR L KVS+ IL +++D+ +G I+GVIGPSGSGKST LR LNRLWEP SG+V+
Sbjct 33 KFRVRNLIKVSESGEAILRGIDVDVPKGAIVGVIGPSGSGKSTLLRVLNRLWEPPSGTVY 92
Query 685 LDGLDICELDVLNLRRKVGMLFQLPALFEGTVADNVRYGPQLRGQKLSDVDVHTLLALAD 506
LDG DI ELDVL LRRKVGMLFQLP LFEGTVADN+RYGPQLRG+KL+D +V LL LAD
Sbjct 93 LDGTDIRELDVLGLRRKVGMLFQLPVLFEGTVADNIRYGPQLRGKKLTDNEVLKLLTLAD 152
Query 505 LDPSFYNKSGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVLLKLKK 326
LD SF K+GGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDV++KLKK
Sbjct 153 LDSSFMKKTGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVIVKLKK 212
Query 325 DRGMTIVMVSHSIKQIQRIADVVCLVVDGEIVEILEPNKLSEAKHPMAKRFLELGS 158
RGMT+VMVSHSIKQIQRIAD VCL+VDGEI+E+LEP+KLSEA+HPMA RFL+L S
Sbjct 213 SRGMTVVMVSHSIKQIQRIADRVCLLVDGEIIEVLEPDKLSEARHPMALRFLQLSS 268
>emb|CAN70271.1| hypothetical protein VITISV_010974 [Vitis vinifera]
Length=251
Score = 359 bits (922), Expect = 1e-121
Identities = 184/236 (78%), Positives = 205/236 (87%), Gaps = 0/236 (0%)
Frame = -1
Query 865 KIEVRGLTKVSDKDVRILNKVNLDIQRGVIMGVIGPSGSGKSTFLRALNRLWEPASGSVF 686
K VR L KVS+ IL +++D+ +G I+GVIGPSGSGKST LR LNRLWEP SG+V+
Sbjct 16 KFRVRNLIKVSESGEAILRGIDVDVPKGAIVGVIGPSGSGKSTLLRVLNRLWEPPSGTVY 75
Query 685 LDGLDICELDVLNLRRKVGMLFQLPALFEGTVADNVRYGPQLRGQKLSDVDVHTLLALAD 506
LDG DI ELDVL LRRKVGMLFQLP LFEGTVADN+RYGPQLRG+KL+D +V LL LAD
Sbjct 76 LDGTDIRELDVLGLRRKVGMLFQLPVLFEGTVADNIRYGPQLRGKKLTDNEVLKLLTLAD 135
Query 505 LDPSFYNKSGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVLLKLKK 326
LD SF K+GGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDV++KLKK
Sbjct 136 LDSSFMKKTGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVIVKLKK 195
Query 325 DRGMTIVMVSHSIKQIQRIADVVCLVVDGEIVEILEPNKLSEAKHPMAKRFLELGS 158
RGMTIVMVSHSIKQIQRIAD VCL+VDGEI E+LEP+KLSEA+HPMA RFL+L S
Sbjct 196 SRGMTIVMVSHSIKQIQRIADRVCLLVDGEITEVLEPDKLSEARHPMALRFLQLSS 251
>ref|XP_003530971.1| PREDICTED: ABC transporter I family member 17-like [Glycine max]
Length=260
Score = 352 bits (902), Expect = 2e-118
Identities = 178/236 (75%), Positives = 204/236 (86%), Gaps = 0/236 (0%)
Frame = -1
Query 865 KIEVRGLTKVSDKDVRILNKVNLDIQRGVIMGVIGPSGSGKSTFLRALNRLWEPASGSVF 686
K +R L++VS+ V IL +NL+I GVI+GVIGPSGSGKSTFLRALNRLWEP S SVF
Sbjct 25 KFLIRNLSRVSEDGVPILKGINLEIPEGVIVGVIGPSGSGKSTFLRALNRLWEPPSASVF 84
Query 685 LDGLDICELDVLNLRRKVGMLFQLPALFEGTVADNVRYGPQLRGQKLSDVDVHTLLALAD 506
LD DIC LDVL+LRR V MLFQLPALFEG+VADNVRYGPQLRG+KLSD +V LL +AD
Sbjct 85 LDAQDICHLDVLSLRRNVAMLFQLPALFEGSVADNVRYGPQLRGKKLSDDEVRKLLLMAD 144
Query 505 LDPSFYNKSGGELSVGQAQRVALARTLANEPEVLLLDEPTSALDPISTQNIEDVLLKLKK 326
LD SF +KSG ELSVGQAQRVALARTLAN P+VLLLDEPTSALDPIST+NIED L+KL K
Sbjct 145 LDASFMDKSGAELSVGQAQRVALARTLANSPQVLLLDEPTSALDPISTENIEDALVKLNK 204
Query 325 DRGMTIVMVSHSIKQIQRIADVVCLVVDGEIVEILEPNKLSEAKHPMAKRFLELGS 158
++GMT++MVSHSIKQIQRIA +VCL+VDGEIVE+L P+ LS+A HPMAKRFL+L S
Sbjct 205 NQGMTVIMVSHSIKQIQRIAHIVCLLVDGEIVEVLNPHNLSQANHPMAKRFLQLSS 260
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 446797928
Number of extensions: 9172181
Number of successful extensions: 46643
Number of sequences better than 1e-10: 4884
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 39311
Number of HSP's successfully gapped: 5248
Length of query: 883
Length of database: 6150218869
Length adjustment: 139
Effective length of query: 744
Effective length of database: 3659466193
Effective search space: 567217259915
Effective search space used: 567217259915
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 176 (72.4 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDW2EBY012
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_19049
Length=296
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002275557.1| PREDICTED: putative expansin-B2 [Vitis vi... 103 8e-25
gb|AAZ08315.1| putative beta-expansin [Eucalyptus globulus] 99.4 8e-24
gb|EAY95094.1| hypothetical protein OsI_16909 [Oryza sativa I... 95.5 5e-22
emb|CAH67407.1| OSIGBa0143N19.1 [Oryza sativa Indica Group] 95.5 5e-22
ref|NP_001053501.2| Os04g0552000 [Oryza sativa Japonica Group... 95.1 7e-22
ALIGNMENTS
>ref|XP_002275557.1| PREDICTED: putative expansin-B2 [Vitis vinifera]
emb|CBI16592.3| unnamed protein product [Vitis vinifera]
Length=273
Score = 103 bits (256), Expect = 8e-25
Identities = 49/96 (51%), Positives = 70/96 (73%), Gaps = 1/96 (1%)
Frame = +3
Query 3 GEADQLRNVRRLQIQYQKVGCDFSGVNVAFRIDPDSKPSYFATAIEYENGDG-LTGVEIR 179
G+ADQLRN LQIQY++V C++ G +VAF +D S P+YFA+ IEYE+GDG L GV+++
Sbjct 146 GQADQLRNAGILQIQYRRVECEYPGASVAFHVDSGSNPNYFASLIEYEDGDGELAGVDLQ 205
Query 180 QANTNGEWLPMELLSGAIFKVNLTEGYTPPFSIKLT 287
QA + W+PM+ GA++K++ G PFSI+LT
Sbjct 206 QALHSNSWVPMQQSWGAVWKLDSGSGLRAPFSIRLT 241
>gb|AAZ08315.1| putative beta-expansin [Eucalyptus globulus]
Length=210
Score = 99.4 bits (246), Expect = 8e-24
Identities = 51/96 (53%), Positives = 65/96 (68%), Gaps = 1/96 (1%)
Frame = +3
Query 3 GEADQLRNVRRLQIQYQKVGCDFSGVNVAFRIDPDSKPSYFATAIEYENGDG-LTGVEIR 179
G+AD+LRN LQIQYQKV C+F G VAF +D S P+YFA IEYE+GDG L V ++
Sbjct 83 GKADELRNAGVLQIQYQKVKCNFPGAKVAFHVDSGSNPNYFAALIEYEDGDGELGAVNLK 142
Query 180 QANTNGEWLPMELLSGAIFKVNLTEGYTPPFSIKLT 287
QA + WLPM+ GA++K++ PFSIKLT
Sbjct 143 QALDSDSWLPMQQSWGAVWKLDGAGQLRAPFSIKLT 178
>gb|EAY95094.1| hypothetical protein OsI_16909 [Oryza sativa Indica Group]
Length=264
Score = 95.5 bits (236), Expect = 5e-22
Identities = 47/99 (47%), Positives = 63/99 (64%), Gaps = 1/99 (1%)
Frame = +3
Query 3 GEADQLRNVRRLQIQYQKVGCDFSGVNVAFRIDPDSKPSYFATAIEYENGDG-LTGVEIR 179
G+ADQLR LQIQY +V C++ GV + F +D S P+YFA ++YENGDG L+GVE+
Sbjct 140 GQADQLRAAGVLQIQYNRVPCNWGGVKLTFVVDAGSNPNYFAVLVKYENGDGDLSGVELM 199
Query 180 QANTNGEWLPMELLSGAIFKVNLTEGYTPPFSIKLTDSS 296
Q W M+ GA++K+N PFSI+LT SS
Sbjct 200 QTGAGAAWTQMQQSWGAVWKLNAGSALQAPFSIRLTSSS 238
>emb|CAH67407.1| OSIGBa0143N19.1 [Oryza sativa Indica Group]
Length=264
Score = 95.5 bits (236), Expect = 5e-22
Identities = 47/99 (47%), Positives = 63/99 (64%), Gaps = 1/99 (1%)
Frame = +3
Query 3 GEADQLRNVRRLQIQYQKVGCDFSGVNVAFRIDPDSKPSYFATAIEYENGDG-LTGVEIR 179
G+ADQLR LQIQY +V C++ GV + F +D S P+YFA ++YENGDG L+GVE+
Sbjct 140 GQADQLRAAGVLQIQYNRVPCNWGGVKLTFVVDAGSNPNYFAVLVKYENGDGDLSGVELM 199
Query 180 QANTNGEWLPMELLSGAIFKVNLTEGYTPPFSIKLTDSS 296
Q W M+ GA++K+N PFSI+LT SS
Sbjct 200 QTGAGAAWTQMQQSWGAVWKLNAGSALQAPFSIRLTSSS 238
>ref|NP_001053501.2| Os04g0552000 [Oryza sativa Japonica Group]
sp|Q7XT40.2|EXB15_ORYSJ RecName: Full=Expansin-B15; AltName: Full=Beta-expansin-15; AltName:
Full=OsEXPB15; AltName: Full=OsaEXPb1.16; Flags: Precursor
gb|AAM73779.1|AF391108_1 beta-expansin OsEXPB15 [Oryza sativa]
emb|CAE01687.2| OSJNBa0010H02.7 [Oryza sativa Japonica Group]
gb|EAZ31563.1| hypothetical protein OsJ_15706 [Oryza sativa Japonica Group]
dbj|BAF15415.2| Os04g0552000 [Oryza sativa Japonica Group]
Length=264
Score = 95.1 bits (235), Expect = 7e-22
Identities = 47/99 (47%), Positives = 63/99 (64%), Gaps = 1/99 (1%)
Frame = +3
Query 3 GEADQLRNVRRLQIQYQKVGCDFSGVNVAFRIDPDSKPSYFATAIEYENGDG-LTGVEIR 179
G+ADQLR LQIQY +V C++ GV + F +D S P+YFA ++YENGDG L+GVE+
Sbjct 140 GQADQLRAAGVLQIQYNRVPCNWGGVKLTFVVDVGSNPNYFAVLVKYENGDGDLSGVELM 199
Query 180 QANTNGEWLPMELLSGAIFKVNLTEGYTPPFSIKLTDSS 296
Q W M+ GA++K+N PFSI+LT SS
Sbjct 200 QTGAGAAWTQMQQSWGAVWKLNAGSALQAPFSIRLTSSS 238
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 3261458740
Number of extensions: 70392146
Number of successful extensions: 165488
Number of sequences better than 1e-10: 150
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 165282
Number of HSP's successfully gapped: 152
Length of query: 296
Length of database: 6150218869
Length adjustment: 67
Effective length of query: 229
Effective length of database: 4949640241
Effective search space: 153438847471
Effective search space used: 153438847471
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCM23EY016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_193
Length=1305
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003635659.1| PREDICTED: cytokinin dehydrogenase 3-like... 407 6e-136
emb|CAN80714.1| hypothetical protein VITISV_042932 [Vitis vin... 409 3e-135
ref|XP_002264445.1| PREDICTED: cytokinin dehydrogenase 3 [Vit... 408 9e-135
emb|CAN77185.1| hypothetical protein VITISV_039458 [Vitis vin... 407 2e-134
emb|CBI37295.3| unnamed protein product [Vitis vinifera] 410 6e-134
ALIGNMENTS
>ref|XP_003635659.1| PREDICTED: cytokinin dehydrogenase 3-like, partial [Vitis vinifera]
Length=418
Score = 407 bits (1047), Expect = 6e-136
Identities = 217/419 (52%), Positives = 279/419 (67%), Gaps = 17/419 (4%)
Frame = +3
Query 3 VRGQAMALNGVVVNMTNEKQIKVG---------LNKTYVDVGAGASWIDVLRATLKYGSA 155
+RGQAMA +GVVV M + G ++ +Y D G WIDVL+ATLK+G A
Sbjct 2 LRGQAMAPHGVVVEMRSLNNCSRGSGIRVTKNPISGSYADAGGEQLWIDVLQATLKHGLA 61
Query 156 PLSWTDYLYLTVGGTLSNAGIGGQTFLHGPQIANVLQLEVVTGKGELVTCSPRIIPELYF 335
P+SWTDYLYLT+GGTLSNAGI GQTF HGPQI+NV +++V+TGKGELVTCS EL+F
Sbjct 62 PVSWTDYLYLTIGGTLSNAGISGQTFRHGPQISNVYEMDVLTGKGELVTCSKDTNSELFF 121
Query 336 GVLGGLGQFGIITRARIVLDKAPKRVKWARLIYSNFAKFARDQEHLISTNASSSYVEGFI 515
VLGGLGQFGII RARI L APKRVKW +++Y +F+ F+RDQEHLIS N Y+EG +
Sbjct 122 AVLGGLGQFGIIIRARIALKPAPKRVKWIQMLYDDFSTFSRDQEHLISIN-GLDYLEGSL 180
Query 516 IVNTSTTDQWIPTFPISPSDQSNISALLNNQSILYAIEVAKYYDDQSTSTIYADFQKLLN 695
++ S+ + W +F SPS+ IS+L++ I+Y +EV KYYD+ ++ T+ + Q+LL
Sbjct 181 LMQNSSPNNWRSSF--SPSEYPRISSLISKNGIIYCLEVVKYYDELTSHTVDEELQELLK 238
Query 696 ELNFNKGLGFITDVSFFDFTNRV----INLDTPETGSLLAHTWLNLFIPKSRIYDFNRLV 863
LNF G F DVS DF NRV +NL + H WLNLF+P SRI DFN V
Sbjct 239 GLNFLPGFVFTKDVSLVDFLNRVHSEELNLRAKGLWD-VPHPWLNLFVPGSRISDFNSGV 297
Query 864 LAGMLPSLKKNPGTFIFYPLNKNKWDDKMSAVTSDEAVFYTLGLLNSSPLDKYQSFTAFN 1043
+LP + + GTF+ YP+ +NKWDD+ SAV DE +FYT+GLL+SS D ++ N
Sbjct 298 FRDILPKINQTTGTFLVYPVIRNKWDDRTSAVIPDEDIFYTVGLLHSSGADDWEPLENQN 357
Query 1044 SKVLSVCKAAGINVKEYLPNYDNMNAWMNHFGTKWVTFSTkkkkfdpkkRLSPGQRIFN 1220
++L C AGI +K YL Y WMNHFG KW TF +K +FDPK LSPGQ+IFN
Sbjct 358 KEILKFCDKAGIKIKRYLSRYTTKKDWMNHFGPKWRTFEDRKAQFDPKMILSPGQQIFN 416
>emb|CAN80714.1| hypothetical protein VITISV_042932 [Vitis vinifera]
Length=522
Score = 409 bits (1052), Expect = 3e-135
Identities = 218/419 (52%), Positives = 278/419 (66%), Gaps = 17/419 (4%)
Frame = +3
Query 3 VRGQAMALNGVVVNMTNEKQIKVGLN---------KTYVDVGAGASWIDVLRATLKYGSA 155
+RGQAMA +GVVV M + G +Y DVG WIDVL+ATLK+G A
Sbjct 106 LRGQAMAXHGVVVEMRSLNNCSXGSGIRVTKNPIWGSYADVGGEQQWIDVLQATLKHGLA 165
Query 156 PLSWTDYLYLTVGGTLSNAGIGGQTFLHGPQIANVLQLEVVTGKGELVTCSPRIIPELYF 335
P+SWTDYLYLT+GGTLSNAGI GQTF HGPQI+NV +++V+TGKGELVTCS EL+F
Sbjct 166 PVSWTDYLYLTIGGTLSNAGISGQTFRHGPQISNVYEMDVLTGKGELVTCSKDTNSELFF 225
Query 336 GVLGGLGQFGIITRARIVLDKAPKRVKWARLIYSNFAKFARDQEHLISTNASSSYVEGFI 515
VLGGLGQFGII RARI L APKRVKW +++Y +F+ F+RDQEHLIS N Y+EG +
Sbjct 226 AVLGGLGQFGIIIRARIALKPAPKRVKWIQMLYDDFSTFSRDQEHLISIN-GLDYLEGSL 284
Query 516 IVNTSTTDQWIPTFPISPSDQSNISALLNNQSILYAIEVAKYYDDQSTSTIYADFQKLLN 695
++ S+ + W +F SPS+ IS+L++ I+Y +EV KYYD+ ++ T+ + Q+LL
Sbjct 285 LMQNSSPNNWRSSF--SPSEYPRISSLISKNGIIYCLEVVKYYDELTSHTVDEELQELLK 342
Query 696 ELNFNKGLGFITDVSFFDFTNRV----INLDTPETGSLLAHTWLNLFIPKSRIYDFNRLV 863
LNF G F DVS DF NRV +NL + H WLNLF+P SRI DFN V
Sbjct 343 GLNFLPGFVFTKDVSLVDFLNRVHSEELNLRAKGLWD-VPHPWLNLFVPGSRISDFNSGV 401
Query 864 LAGMLPSLKKNPGTFIFYPLNKNKWDDKMSAVTSDEAVFYTLGLLNSSPLDKYQSFTAFN 1043
+LP + + GTF+ YP+ +NKWDD+ SAV DE +FYT+GLL+SS D ++ N
Sbjct 402 FRDILPKINQTTGTFLVYPVIRNKWDDRTSAVIPDEDIFYTVGLLHSSGADDWEPLENQN 461
Query 1044 SKVLSVCKAAGINVKEYLPNYDNMNAWMNHFGTKWVTFSTkkkkfdpkkRLSPGQRIFN 1220
++L C AGI +K YL Y WMNHFG KW TF +K +FDPK LSPGQ+IFN
Sbjct 462 KEILKFCDKAGIKIKRYLSRYTTKKDWMNHFGPKWRTFEDRKAQFDPKMILSPGQQIFN 520
>ref|XP_002264445.1| PREDICTED: cytokinin dehydrogenase 3 [Vitis vinifera]
Length=522
Score = 408 bits (1049), Expect = 9e-135
Identities = 217/419 (52%), Positives = 279/419 (67%), Gaps = 17/419 (4%)
Frame = +3
Query 3 VRGQAMALNGVVVNMTNEKQIKVG---------LNKTYVDVGAGASWIDVLRATLKYGSA 155
+RGQAMA +GVVV M + G ++ +Y D G WIDVL+ATLK+G A
Sbjct 106 LRGQAMAPHGVVVEMRSLNNCSRGSGIRVTKNPISGSYADAGGEQLWIDVLQATLKHGLA 165
Query 156 PLSWTDYLYLTVGGTLSNAGIGGQTFLHGPQIANVLQLEVVTGKGELVTCSPRIIPELYF 335
P+SWTDYLYLT+GGTLSNAGI GQTF HGPQI+NV +++V+TGKGELVTCS EL+F
Sbjct 166 PVSWTDYLYLTIGGTLSNAGISGQTFRHGPQISNVYEMDVLTGKGELVTCSKDTNSELFF 225
Query 336 GVLGGLGQFGIITRARIVLDKAPKRVKWARLIYSNFAKFARDQEHLISTNASSSYVEGFI 515
VLGGLGQFGII RARI L APKRVKW +++Y +F+ F+RDQEHLIS N Y+EG +
Sbjct 226 AVLGGLGQFGIIIRARIALKPAPKRVKWIQMLYDDFSTFSRDQEHLISIN-GLDYLEGSL 284
Query 516 IVNTSTTDQWIPTFPISPSDQSNISALLNNQSILYAIEVAKYYDDQSTSTIYADFQKLLN 695
++ S+ + W +F SPS+ IS+L++ I+Y +EV KYYD+ ++ T+ + Q+LL
Sbjct 285 LMQNSSPNNWRSSF--SPSEYPRISSLISKNGIIYCLEVVKYYDELTSHTVDEELQELLK 342
Query 696 ELNFNKGLGFITDVSFFDFTNRV----INLDTPETGSLLAHTWLNLFIPKSRIYDFNRLV 863
LNF G F DVS DF NRV +NL + H WLNLF+P SRI DFN V
Sbjct 343 GLNFLPGFVFTKDVSLVDFLNRVHSEELNLQAKGLWD-VPHPWLNLFVPGSRISDFNSGV 401
Query 864 LAGMLPSLKKNPGTFIFYPLNKNKWDDKMSAVTSDEAVFYTLGLLNSSPLDKYQSFTAFN 1043
+LP + + GTF+ YP+ +NKWDD+ SAV DE +FYT+GLL+SS D ++ N
Sbjct 402 FRDILPKINQTTGTFLVYPVIRNKWDDRTSAVIPDEDIFYTVGLLHSSGADDWEPLENQN 461
Query 1044 SKVLSVCKAAGINVKEYLPNYDNMNAWMNHFGTKWVTFSTkkkkfdpkkRLSPGQRIFN 1220
++L C AGI +K YL Y WMNHFG KW TF +K +FDPK LSPGQ+IFN
Sbjct 462 KEILQFCDKAGIKIKRYLSRYTTKEDWMNHFGPKWRTFEDRKAQFDPKLILSPGQQIFN 520
>emb|CAN77185.1| hypothetical protein VITISV_039458 [Vitis vinifera]
Length=522
Score = 407 bits (1047), Expect = 2e-134
Identities = 217/419 (52%), Positives = 277/419 (66%), Gaps = 17/419 (4%)
Frame = +3
Query 3 VRGQAMALNGVVVNMTNEKQIKVGLN---------KTYVDVGAGASWIDVLRATLKYGSA 155
+RGQAMA +GVVV M + G +Y DVG WIDVL+ATLK+G A
Sbjct 106 LRGQAMASHGVVVEMRSLNNCSCGSGIRVTKNPIWGSYADVGGEQQWIDVLQATLKHGLA 165
Query 156 PLSWTDYLYLTVGGTLSNAGIGGQTFLHGPQIANVLQLEVVTGKGELVTCSPRIIPELYF 335
P+SWTDYLYLT+GGTLSNAGI GQTF HGPQI+NV +++V+TGKGELVTCS EL+F
Sbjct 166 PVSWTDYLYLTIGGTLSNAGISGQTFRHGPQISNVYEMDVLTGKGELVTCSKDTNSELFF 225
Query 336 GVLGGLGQFGIITRARIVLDKAPKRVKWARLIYSNFAKFARDQEHLISTNASSSYVEGFI 515
VLGGLGQFGIITRARI L+ AP+RVKW +++Y +F+ F+RDQEHLIS N Y+EG +
Sbjct 226 AVLGGLGQFGIITRARIALEPAPERVKWIQMLYDDFSTFSRDQEHLISIN-GLDYLEGSL 284
Query 516 IVNTSTTDQWIPTFPISPSDQSNISALLNNQSILYAIEVAKYYDDQSTSTIYADFQKLLN 695
+ S + W +F SPS+ IS+L++ I+Y +EV KYYD+ ++ T+ + Q+LL
Sbjct 285 XMQNSXPNNWRSSF--SPSEYPRISSLISKNGIIYCLEVVKYYDELTSHTVDEELQELLK 342
Query 696 ELNFNKGLGFITDVSFFDFTNRV----INLDTPETGSLLAHTWLNLFIPKSRIYDFNRLV 863
LNF G F DVS DF NRV +NL + H WLNLF+P SRI DFN V
Sbjct 343 GLNFLPGFVFTKDVSLVDFLNRVHSEELNLRAKGLWD-VPHPWLNLFVPGSRISDFNSGV 401
Query 864 LAGMLPSLKKNPGTFIFYPLNKNKWDDKMSAVTSDEAVFYTLGLLNSSPLDKYQSFTAFN 1043
+LP + + GTF+ YP+ +NKWDD+ SAV DE +FYT+G L+SS D ++ N
Sbjct 402 FRDILPKINQTTGTFLVYPVIRNKWDDRTSAVIPDEDIFYTVGXLHSSGADDWEPLENQN 461
Query 1044 SKVLSVCKAAGINVKEYLPNYDNMNAWMNHFGTKWVTFSTkkkkfdpkkRLSPGQRIFN 1220
++L C AGI +K YL Y WMNHFG KW TF +K +FDPK LSPGQ+IFN
Sbjct 462 KEILKFCDKAGIKIKRYLSRYTTKXDWMNHFGPKWXTFEDRKAQFDPKMILSPGQQIFN 520
>emb|CBI37295.3| unnamed protein product [Vitis vinifera]
Length=632
Score = 410 bits (1053), Expect = 6e-134
Identities = 218/419 (52%), Positives = 278/419 (66%), Gaps = 17/419 (4%)
Frame = +3
Query 3 VRGQAMALNGVVVNMTNEKQIKVGLN---------KTYVDVGAGASWIDVLRATLKYGSA 155
+RGQAMA +GVVV M + G +Y DVG WIDVL+ATLK+G A
Sbjct 216 LRGQAMASHGVVVEMRSLNNCSCGSGIRVTKNPIWGSYADVGGEQQWIDVLQATLKHGLA 275
Query 156 PLSWTDYLYLTVGGTLSNAGIGGQTFLHGPQIANVLQLEVVTGKGELVTCSPRIIPELYF 335
P+SWTDYLYLT+GGTLSNAGI GQTF HGPQI+NV +++V+TGKGELVTCS EL+F
Sbjct 276 PVSWTDYLYLTIGGTLSNAGISGQTFRHGPQISNVYEMDVLTGKGELVTCSKDTNSELFF 335
Query 336 GVLGGLGQFGIITRARIVLDKAPKRVKWARLIYSNFAKFARDQEHLISTNASSSYVEGFI 515
VLGGLGQFGII RARI L APKRVKW +++Y +F+ F+RDQEHLIS N Y+EG +
Sbjct 336 AVLGGLGQFGIIIRARIALKPAPKRVKWIQMLYDDFSTFSRDQEHLISIN-GLDYLEGSL 394
Query 516 IVNTSTTDQWIPTFPISPSDQSNISALLNNQSILYAIEVAKYYDDQSTSTIYADFQKLLN 695
++ S+ + W +F SPS+ IS+L++ I+Y +EV KYYD+ ++ T+ + Q+LL
Sbjct 395 LMQNSSPNNWRSSF--SPSEYPRISSLISKNGIIYCLEVVKYYDELTSHTVDEELQELLK 452
Query 696 ELNFNKGLGFITDVSFFDFTNRV----INLDTPETGSLLAHTWLNLFIPKSRIYDFNRLV 863
LNF G F DVS DF NRV +NL + H WLNLF+P SRI DFN V
Sbjct 453 GLNFLPGFVFTKDVSLVDFLNRVHSEELNLQAKGLWD-VPHPWLNLFVPGSRISDFNSGV 511
Query 864 LAGMLPSLKKNPGTFIFYPLNKNKWDDKMSAVTSDEAVFYTLGLLNSSPLDKYQSFTAFN 1043
+LP + + GTF+ YP+ +NKWDD+ SAV DE +FYT+GLL+SS D ++ N
Sbjct 512 FRDILPKINQTTGTFLVYPVIRNKWDDRTSAVIPDEDIFYTVGLLHSSGADDWEPLENQN 571
Query 1044 SKVLSVCKAAGINVKEYLPNYDNMNAWMNHFGTKWVTFSTkkkkfdpkkRLSPGQRIFN 1220
++L C AGI +K YL Y WMNHFG KW TF +K +FDPK LSPGQ+IFN
Sbjct 572 KEILQFCDKAGIKIKRYLSRYTTKEDWMNHFGPKWRTFEDRKAQFDPKLILSPGQQIFN 630
Score = 115 bits (289), Expect = 5e-25
Identities = 58/102 (57%), Positives = 73/102 (72%), Gaps = 9/102 (9%)
Frame = +3
Query 3 VRGQAMALNGVVVNMTNEKQIKVG---------LNKTYVDVGAGASWIDVLRATLKYGSA 155
+RGQAMA +GVVV M + G ++ +Y D G WIDVL+ATLK+G A
Sbjct 106 LRGQAMAPHGVVVEMRSLNNCSRGSGIRVTKNPISGSYADAGGEQLWIDVLQATLKHGLA 165
Query 156 PLSWTDYLYLTVGGTLSNAGIGGQTFLHGPQIANVLQLEVVT 281
P+SWTDYLYLT+GGTLSNAGI GQTF HGPQI+NV +++V+T
Sbjct 166 PVSWTDYLYLTIGGTLSNAGISGQTFRHGPQISNVYEMDVLT 207
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 13964863820
Number of extensions: 302558797
Number of successful extensions: 765889
Number of sequences better than 1e-10: 275
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 763282
Number of HSP's successfully gapped: 283
Length of query: 1305
Length of database: 6150218869
Length adjustment: 144
Effective length of query: 1161
Effective length of database: 3569870773
Effective search space: 1038832394943
Effective search space used: 1038832394943
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD254P201S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_2144
Length=847
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003618435.1| FACT complex subunit SPT16 [Medicago trun... 156 2e-39
ref|XP_003552890.1| PREDICTED: FACT complex subunit SPT16-lik... 156 2e-39
ref|XP_003553020.1| PREDICTED: FACT complex subunit SPT16-lik... 155 3e-39
ref|XP_003538371.1| PREDICTED: FACT complex subunit SPT16-lik... 155 3e-39
gb|ADE76954.1| unknown [Picea sitchensis] 147 3e-38
ALIGNMENTS
>ref|XP_003618435.1| FACT complex subunit SPT16 [Medicago truncatula]
gb|AES74653.1| FACT complex subunit SPT16 [Medicago truncatula]
Length=1058
Score = 156 bits (394), Expect = 2e-39
Identities = 102/128 (80%), Positives = 116/128 (91%), Gaps = 0/128 (0%)
Frame = -2
Query 846 PSSSLDGIKEWLDTTDIKYYESRLNLNWRPILKTITDDPQQFIEEGGWEFLNLEATDSDT 667
PS+S+DGIKEWLDTTDIKYYESRLNLNWR ILKTITDDPQ FIE GGWEFLNLEA DS++
Sbjct 896 PSTSIDGIKEWLDTTDIKYYESRLNLNWRQILKTITDDPQSFIEGGGWEFLNLEAADSES 955
Query 666 DNSAESDKGYepsdaepesdseeeasdseslvesedddededsedeEQGKTWEELEREAS 487
+ S ESDKGYEPSD EPESDSEEE S+S SLVESE++++ E+ +EEQGKTWEELER+AS
Sbjct 956 EGSEESDKGYEPSDIEPESDSEEEDSESASLVESEEEEDSEEDSEEEQGKTWEELERDAS 1015
Query 486 NADKEKGN 463
NAD+EKGN
Sbjct 1016 NADREKGN 1023
>ref|XP_003552890.1| PREDICTED: FACT complex subunit SPT16-like [Glycine max]
Length=1064
Score = 156 bits (394), Expect = 2e-39
Identities = 110/130 (85%), Positives = 119/130 (92%), Gaps = 2/130 (2%)
Frame = -2
Query 846 PSSSLDGIKEWLDTTDIKYYESRLNLNWRPILKTITDDPQQFIEEGGWEFLNLEATDSDT 667
PS+SLDGIKEWLDTTDIKYYESRLNLNWR ILKTITDDPQ FIE GGWEFLNLEATDS++
Sbjct 900 PSTSLDGIKEWLDTTDIKYYESRLNLNWRQILKTITDDPQSFIEGGGWEFLNLEATDSES 959
Query 666 DNSAESDKGYepsdaepesdseeeasdseslvesedddededsedeEQ--GKTWEELERE 493
+NS ESDKGYEPSD EPESDSE+EASDSESLVESEDD E++ ED E+ GKTWEELERE
Sbjct 960 ENSEESDKGYEPSDVEPESDSEDEASDSESLVESEDDGEEDSEEDSEEEKGKTWEELERE 1019
Query 492 ASNADKEKGN 463
ASNAD+EKGN
Sbjct 1020 ASNADREKGN 1029
>ref|XP_003553020.1| PREDICTED: FACT complex subunit SPT16-like [Glycine max]
Length=1068
Score = 155 bits (393), Expect = 3e-39
Identities = 112/131 (85%), Positives = 122/131 (93%), Gaps = 3/131 (2%)
Frame = -2
Query 846 PSSSLDGIKEWLDTTDIKYYESRLNLNWRPILKTITDDPQQFIEEGGWEFLNLEATDSDT 667
PS+SLDGIKEWLDTTDIKYYESRLNLNWR ILKTITDDPQ FIE GGWEFLNLEATDS++
Sbjct 903 PSTSLDGIKEWLDTTDIKYYESRLNLNWRQILKTITDDPQSFIEGGGWEFLNLEATDSES 962
Query 666 DNSAESDKGYepsdaepesdseeeasdseslvesedddededsedeEQ---GKTWEELER 496
+NS ESDKGYEPSD EPESDSE+EASDSESLVESEDDD+DEDSE++ + GKTWEELER
Sbjct 963 ENSEESDKGYEPSDVEPESDSEDEASDSESLVESEDDDDDEDSEEDSEEEKGKTWEELER 1022
Query 495 EASNADKEKGN 463
EASNAD+EKGN
Sbjct 1023 EASNADREKGN 1033
>ref|XP_003538371.1| PREDICTED: FACT complex subunit SPT16-like [Glycine max]
Length=1068
Score = 155 bits (393), Expect = 3e-39
Identities = 112/131 (85%), Positives = 122/131 (93%), Gaps = 3/131 (2%)
Frame = -2
Query 846 PSSSLDGIKEWLDTTDIKYYESRLNLNWRPILKTITDDPQQFIEEGGWEFLNLEATDSDT 667
PS+SLDGIKEWLDTTDIKYYESRLNLNWR ILKTITDDPQ FIE GGWEFLNLEATDS++
Sbjct 903 PSTSLDGIKEWLDTTDIKYYESRLNLNWRQILKTITDDPQSFIEGGGWEFLNLEATDSES 962
Query 666 DNSAESDKGYepsdaepesdseeeasdseslvesedddededsedeEQ---GKTWEELER 496
+NS ESDKGYEPSD EPESDSE+EASDSESLVESEDDD+DEDSE++ + GKTWEELER
Sbjct 963 ENSEESDKGYEPSDVEPESDSEDEASDSESLVESEDDDDDEDSEEDSEEEKGKTWEELER 1022
Query 495 EASNADKEKGN 463
EASNAD+EKGN
Sbjct 1023 EASNADREKGN 1033
>gb|ADE76954.1| unknown [Picea sitchensis]
Length=372
Score = 147 bits (372), Expect = 3e-38
Identities = 93/128 (73%), Positives = 118/128 (92%), Gaps = 0/128 (0%)
Frame = -2
Query 846 PSSSLDGIKEWLDTTDIKYYESRLNLNWRPILKTITDDPQQFIEEGGWEFLNLEATDSDT 667
PS+SLDGIKEWL++ DIKYYESR+NLNWRPILKTITDDP++FIE+GGWEFLN+EA+DSD+
Sbjct 208 PSASLDGIKEWLNSMDIKYYESRMNLNWRPILKTITDDPEKFIEDGGWEFLNMEASDSDS 267
Query 666 DNSAESDKGYepsdaepesdseeeasdseslvesedddededsedeEQGKTWEELEREAS 487
+ S ESD+GYEPSD E ES+S+EE S++ESLVES++D+E+E+ +EE+GKTW+ELE EA
Sbjct 268 EGSEESDQGYEPSDVEEESESDEEDSENESLVESDEDEEEEEDSEEEEGKTWDELEAEAR 327
Query 486 NADKEKGN 463
NADKEKG+
Sbjct 328 NADKEKGD 335
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 320334330
Number of extensions: 5543186
Number of successful extensions: 14563
Number of sequences better than 1e-10: 7
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 14546
Number of HSP's successfully gapped: 7
Length of query: 847
Length of database: 6150218869
Length adjustment: 139
Effective length of query: 708
Effective length of database: 3659466193
Effective search space: 523303665599
Effective search space used: 523303665599
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 176 (72.4 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD2724001S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_2150
Length=846
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002304535.1| predicted protein [Populus trichocarpa] >... 206 4e-63
ref|NP_176598.1| Disease resistance-responsive (dirigent-like... 187 2e-55
ref|XP_002887917.1| disease resistance-responsive family prot... 186 5e-55
gb|ACC91257.1| disease resistance-responsive family protein [... 184 2e-54
gb|ABV89607.1| disease resistance response protein [Brassica ... 184 3e-54
ALIGNMENTS
>ref|XP_002304535.1| predicted protein [Populus trichocarpa]
gb|EEE79514.1| predicted protein [Populus trichocarpa]
Length=162
Score = 206 bits (524), Expect = 4e-63
Identities = 112/165 (68%), Positives = 129/165 (78%), Gaps = 3/165 (2%)
Frame = -2
Query 710 SCSSSVKSLKQNKPCKQMVLYCHDILFNGTDQanatsaattnstllalNPSGHFGAIVVF 531
S SS+ KS K +PCK +VLY HD LFNGTD ANATSAA T+ T L FG +VVF
Sbjct 1 SVSSAKKSFKPKQPCKDLVLYYHDTLFNGTDVANATSAAATDPTKLGNFK---FGMLVVF 57
Query 530 NDPVTKDNRLQSEPVAQAQGFYIYNKKEEANAWFAFTLVFNSTEYKGTLNIMGADIMNAK 351
+DP+TKDN L S PVA+AQGFY Y+KK AWFAFTL+FNST++KGTLNIMGAD+M +
Sbjct 58 DDPMTKDNHLLSRPVARAQGFYFYDKKSTYTAWFAFTLIFNSTKHKGTLNIMGADLMTEE 117
Query 350 TRDFSVVGGTGDFFMARGICTVSTDVLQGGFYFRLILDIKLYECY 216
TRDFSVVGGTGDFFMARGI T+ TD QG +YFRL +DIKLYECY
Sbjct 118 TRDFSVVGGTGDFFMARGIATIHTDTFQGDYYFRLKMDIKLYECY 162
>ref|NP_176598.1| Disease resistance-responsive (dirigent-like protein) family
protein [Arabidopsis thaliana]
gb|AAF24580.1|AC007764_22 F22C12.8 [Arabidopsis thaliana]
gb|AAQ65109.1| At1g64160 [Arabidopsis thaliana]
dbj|BAD43018.1| putative dirigent protein [Arabidopsis thaliana]
dbj|BAD44501.1| putative dirigent protein [Arabidopsis thaliana]
gb|AEE34203.1| Disease resistance-responsive (dirigent-like protein) family
protein [Arabidopsis thaliana]
Length=182
Score = 187 bits (475), Expect = 2e-55
Identities = 98/179 (55%), Positives = 122/179 (68%), Gaps = 13/179 (7%)
Frame = -2
Query 740 LFLIVLLTIISCSSSVKSLKQNKPCKQMVLYCHDILFNGTDQanatsaattnstllalNP 561
+FL++ T+IS KS Q KPCK VLY HDI+F D ++ NP
Sbjct 13 VFLVLTKTVISARKPSKS--QPKPCKNFVLYYHDIMFGVDD-------VQNATSAAVTNP 63
Query 560 SG----HFGAIVVFNDPVTKDNRLQSEPVAQAQGFYIYNKKEEANAWFAFTLVFNSTEYK 393
G FG +V+F+DP+T D QSEPVA+AQGFY Y+ K + NAWFA+TLVFNST++K
Sbjct 64 PGLGNFKFGKLVIFDDPMTIDKNFQSEPVARAQGFYFYDMKNDYNAWFAYTLVFNSTQHK 123
Query 392 GTLNIMGADIMNAKTRDFSVVGGTGDFFMARGICTVSTDVLQGGFYFRLILDIKLYECY 216
GTLNIMGAD+M ++RD SVVGGTGDFFM+RGI T TD +G YFR+ +DIKLYECY
Sbjct 124 GTLNIMGADLMMVQSRDLSVVGGTGDFFMSRGIVTFETDTFEGAKYFRVKMDIKLYECY 182
>ref|XP_002887917.1| disease resistance-responsive family protein [Arabidopsis lyrata
subsp. lyrata]
gb|EFH64176.1| disease resistance-responsive family protein [Arabidopsis lyrata
subsp. lyrata]
Length=182
Score = 186 bits (472), Expect = 5e-55
Identities = 97/179 (54%), Positives = 122/179 (68%), Gaps = 13/179 (7%)
Frame = -2
Query 740 LFLIVLLTIISCSSSVKSLKQNKPCKQMVLYCHDILFNGTDQanatsaattnstllalNP 561
+FL++ T+IS KS Q KPCK VLY HDI+F D ++ NP
Sbjct 13 IFLVLSKTVISARKPSKS--QPKPCKNFVLYYHDIMFGVDD-------VQNATSAAITNP 63
Query 560 SG----HFGAIVVFNDPVTKDNRLQSEPVAQAQGFYIYNKKEEANAWFAFTLVFNSTEYK 393
G FG +V+F+DP+T D QSEPVA+AQGFY Y+ K + NAWFA+TLVFNST++K
Sbjct 64 PGLGNFKFGKLVIFDDPMTIDKNFQSEPVARAQGFYFYDMKNDYNAWFAYTLVFNSTQHK 123
Query 392 GTLNIMGADIMNAKTRDFSVVGGTGDFFMARGICTVSTDVLQGGFYFRLILDIKLYECY 216
GTLNIMGAD+M ++RD SVVGGTGDFFM+RGI T TD +G YFR+ +DIKLY+CY
Sbjct 124 GTLNIMGADLMMVQSRDLSVVGGTGDFFMSRGIVTFETDTFEGAKYFRVKMDIKLYDCY 182
>gb|ACC91257.1| disease resistance-responsive family protein [Capsella rubella]
Length=187
Score = 184 bits (468), Expect = 2e-54
Identities = 96/178 (54%), Positives = 120/178 (67%), Gaps = 11/178 (6%)
Frame = -2
Query 737 FLIVLLTIISCSSSVKSLKQNKPCKQMVLYCHDILFNGTDQanatsaattnstllalNPS 558
FL+VLL + S ++L Q KPCK Y HDIL++G + ++ ++P
Sbjct 17 FLLVLLFSDTVFGSRRTLDQKKPCKHFSFYFHDILYDGDN-------VANATSAAIVSPP 69
Query 557 G----HFGAIVVFNDPVTKDNRLQSEPVAQAQGFYIYNKKEEANAWFAFTLVFNSTEYKG 390
G FG V+F+DP+T D SEPVA+AQGFY Y+ K + NAWF +TLVFNST++KG
Sbjct 70 GLGNFKFGKFVIFDDPITMDKNYLSEPVARAQGFYFYDMKMDFNAWFCYTLVFNSTQHKG 129
Query 389 TLNIMGADIMNAKTRDFSVVGGTGDFFMARGICTVSTDVLQGGFYFRLILDIKLYECY 216
TLNIMGAD+M TRD SVVGGTGDFFMARGI T TD+ QG YFR+ +DIKLYECY
Sbjct 130 TLNIMGADLMMEPTRDLSVVGGTGDFFMARGIATFVTDLFQGAKYFRVKMDIKLYECY 187
>gb|ABV89607.1| disease resistance response protein [Brassica rapa]
Length=187
Score = 184 bits (467), Expect = 3e-54
Identities = 97/178 (54%), Positives = 120/178 (67%), Gaps = 11/178 (6%)
Frame = -2
Query 737 FLIVLLTIISCSSSVKSLKQNKPCKQMVLYCHDILFNGTDQanatsaattnstllalNPS 558
FL+VLL + S K+L Q KPCK Y HDIL++G + ++ ++P
Sbjct 17 FLLVLLFSDTVYSFRKTLDQRKPCKHFSFYFHDILYDGDN-------VANATSAAIVSPP 69
Query 557 G----HFGAIVVFNDPVTKDNRLQSEPVAQAQGFYIYNKKEEANAWFAFTLVFNSTEYKG 390
G FG V+F+ P+T D SEPVA+AQGFY Y+ K + N+WF+FTLVFNSTE+KG
Sbjct 70 GLGNFKFGKFVIFDGPITMDKNYLSEPVARAQGFYFYDMKTDFNSWFSFTLVFNSTEHKG 129
Query 389 TLNIMGADIMNAKTRDFSVVGGTGDFFMARGICTVSTDVLQGGFYFRLILDIKLYECY 216
TLNIMGAD+M TRD SVVGGTGDFFMARGI T TD+ QG YFR+ +DIKLYECY
Sbjct 130 TLNIMGADLMMEPTRDLSVVGGTGDFFMARGIATFVTDLFQGARYFRVKMDIKLYECY 187
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 468283081
Number of extensions: 10447263
Number of successful extensions: 25613
Number of sequences better than 1e-10: 2
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 25560
Number of HSP's successfully gapped: 2
Length of query: 846
Length of database: 6150218869
Length adjustment: 139
Effective length of query: 707
Effective length of database: 3659466193
Effective search space: 523303665599
Effective search space used: 523303665599
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 176 (72.4 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD33H2T012
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_2405
Length=823
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002527782.1| Pectinesterase-1 precursor, putative [Ric... 337 2e-109
ref|XP_002314796.1| predicted protein [Populus trichocarpa] >... 323 7e-104
emb|CAN77092.1| hypothetical protein VITISV_026596 [Vitis vin... 315 4e-101
ref|XP_003630894.1| Pectinesterase [Medicago truncatula] >gb|... 301 3e-98
ref|XP_003530788.1| PREDICTED: probable pectinesterase/pectin... 306 1e-97
ALIGNMENTS
>ref|XP_002527782.1| Pectinesterase-1 precursor, putative [Ricinus communis]
gb|EEF34592.1| Pectinesterase-1 precursor, putative [Ricinus communis]
Length=529
Score = 337 bits (864), Expect = 2e-109
Identities = 158/210 (75%), Positives = 176/210 (84%), Gaps = 5/210 (2%)
Frame = -1
Query 823 QKHQAVAFRSGSDFSVFYTCSFKGYQDTLYLYAQRQFYRNCDIYGTVDFIFGDAVAVIQN 644
QKHQAVA RSGSDFSVFY CSFKGYQDTLY+Y+QRQFYR+CDIYGT+DFIFGDAVAV+QN
Sbjct 320 QKHQAVALRSGSDFSVFYGCSFKGYQDTLYVYSQRQFYRDCDIYGTIDFIFGDAVAVLQN 379
Query 643 SNIYVRKPMPNQKNTVTAQSRKDPNQNTGIVIHASRVSTSA-----ASGFENYLGRPWQR 479
NIYVR+PM QKNTVTAQ RKDPN+NTGIVIH S V ++ F+ YLGRPWQ+
Sbjct 380 CNIYVRRPMNGQKNTVTAQGRKDPNENTGIVIHNSNVMATSDMRPVQGSFKTYLGRPWQK 439
Query 478 YSRTVFMKCEIDGGIAAAGWLPWSGDFGLSTLYYGEYMNTGSGASTGGRVKWPGYHVIGS 299
YSRT+FMK +DG I AGWLPWSG+F LSTLYYGEYMNTGSGAST RV WPGYHVI
Sbjct 440 YSRTLFMKSNLDGLIDPAGWLPWSGNFALSTLYYGEYMNTGSGASTARRVNWPGYHVITK 499
Query 298 GVEAGKFSVGSFLGGDSWIPGTGVPFTSGV 209
+AGKF+VG+FL GDSWIPGTGVPF SG+
Sbjct 500 ATDAGKFTVGNFLAGDSWIPGTGVPFDSGL 529
>ref|XP_002314796.1| predicted protein [Populus trichocarpa]
gb|EEF00967.1| predicted protein [Populus trichocarpa]
Length=531
Score = 323 bits (827), Expect = 7e-104
Identities = 153/210 (73%), Positives = 174/210 (83%), Gaps = 5/210 (2%)
Frame = -1
Query 823 QKHQAVAFRSGSDFSVFYTCSFKGYQDTLYLYAQRQFYRNCDIYGTVDFIFGDAVAVIQN 644
QKHQAVA RSGSDFSVFY+CSFKGYQDTLY+++QRQFYR+CDIYGTVDFIFGDAVAV+QN
Sbjct 322 QKHQAVALRSGSDFSVFYSCSFKGYQDTLYVHSQRQFYRDCDIYGTVDFIFGDAVAVLQN 381
Query 643 SNIYVRKPMPNQKNTVTAQSRKDPNQNTGIVIHASRVSTS-----AASGFENYLGRPWQR 479
NIYVR+PM Q NTVTAQ R DP++NTGIVIH SRV + F++YLGRPW++
Sbjct 382 CNIYVRRPMSKQTNTVTAQGRTDPDENTGIVIHNSRVMAAPDLRPVQGSFKSYLGRPWKK 441
Query 478 YSRTVFMKCEIDGGIAAAGWLPWSGDFGLSTLYYGEYMNTGSGASTGGRVKWPGYHVIGS 299
YSRTVF+K IDG I AGWLPW GDF LSTLYYGEYM+TGSGAST GRVKWPGYH I S
Sbjct 442 YSRTVFLKSNIDGLIDPAGWLPWKGDFALSTLYYGEYMSTGSGASTKGRVKWPGYHTITS 501
Query 298 GVEAGKFSVGSFLGGDSWIPGTGVPFTSGV 209
+EAGKF+V +FL G+SWI GVPF SG+
Sbjct 502 PLEAGKFTVENFLAGNSWISAAGVPFESGL 531
>emb|CAN77092.1| hypothetical protein VITISV_026596 [Vitis vinifera]
Length=507
Score = 315 bits (807), Expect = 4e-101
Identities = 151/210 (72%), Positives = 167/210 (80%), Gaps = 5/210 (2%)
Frame = -1
Query 823 QKHQAVAFRSGSDFSVFYTCSFKGYQDTLYLYAQRQFYRNCDIYGTVDFIFGDAVAVIQN 644
QKHQAVA RS SD SVFY CSFKGYQDTLY++ QRQFYR+CD+YGTVDFIFGDAVAV+QN
Sbjct 298 QKHQAVALRSSSDGSVFYGCSFKGYQDTLYVHTQRQFYRSCDVYGTVDFIFGDAVAVLQN 357
Query 643 SNIYVRKPMPNQKNTVTAQSRKDPNQNTGIVIHASRVSTS-----AASGFENYLGRPWQR 479
NIYVR+PM NQ N +TAQ R D N+NTGI IH SRV + S F+ YLGRPW++
Sbjct 358 CNIYVRRPMSNQPNVITAQGRSDQNENTGISIHNSRVMAAPDLRPVQSRFKTYLGRPWRK 417
Query 478 YSRTVFMKCEIDGGIAAAGWLPWSGDFGLSTLYYGEYMNTGSGASTGGRVKWPGYHVIGS 299
YSRTVFMK +DG I GW PW GDFGLSTLYYGEYMNTGSGAST GRVKW GYHVI S
Sbjct 418 YSRTVFMKTSLDGLIHPEGWSPWKGDFGLSTLYYGEYMNTGSGASTRGRVKWRGYHVITS 477
Query 298 GVEAGKFSVGSFLGGDSWIPGTGVPFTSGV 209
EA KF+VG FL GDSWIP TGVP+ SG+
Sbjct 478 AAEADKFTVGRFLVGDSWIPTTGVPYASGL 507
>ref|XP_003630894.1| Pectinesterase [Medicago truncatula]
gb|AET05370.1| Pectinesterase [Medicago truncatula]
Length=326
Score = 301 bits (772), Expect = 3e-98
Identities = 140/206 (68%), Positives = 166/206 (81%), Gaps = 5/206 (2%)
Frame = -1
Query 823 QKHQAVAFRSGSDFSVFYTCSFKGYQDTLYLYAQRQFYRNCDIYGTVDFIFGDAVAVIQN 644
QKHQAVA RSG+D SVFY C+FKG+QDTLY+YA RQFYR+C+IYGT+DFIFG+AV V+QN
Sbjct 100 QKHQAVALRSGADHSVFYRCAFKGFQDTLYVYANRQFYRDCNIYGTIDFIFGNAVTVLQN 159
Query 643 SNIYVRKPMPNQKNTVTAQSRKDPNQNTGIVIHASRVSTSA-----ASGFENYLGRPWQR 479
NI+VRKPM NQ+NTVTAQ R DPN+NTGIVIH R++ S+ + + YLGRPWQ+
Sbjct 160 CNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITASSDLKAIQNSVKTYLGRPWQK 219
Query 478 YSRTVFMKCEIDGGIAAAGWLPWSGDFGLSTLYYGEYMNTGSGASTGGRVKWPGYHVIGS 299
YSRTV MK +DG I + GW PW G F LSTLYYGEYMN G GA+T GRVKWPG+HVI +
Sbjct 220 YSRTVVMKSNLDGLINSEGWAPWMGGFALSTLYYGEYMNVGGGANTDGRVKWPGFHVITN 279
Query 298 GVEAGKFSVGSFLGGDSWIPGTGVPF 221
+A KFSVG+FL GDSWI G+GVPF
Sbjct 280 PSDAVKFSVGNFLAGDSWISGSGVPF 305
>ref|XP_003530788.1| PREDICTED: probable pectinesterase/pectinesterase inhibitor 6-like
[Glycine max]
Length=526
Score = 306 bits (785), Expect = 1e-97
Identities = 145/210 (69%), Positives = 168/210 (80%), Gaps = 6/210 (3%)
Frame = -1
Query 823 QKHQAVAFRSGSDFSVFYTCSFKGYQDTLYLYAQRQFYRNCDIYGTVDFIFGDAVAVIQN 644
QKHQAVA RSG+D SVFY CSF+GYQDTLY+YA RQFYR+CDIYGTVDFIFGDAVAV+QN
Sbjct 318 QKHQAVALRSGADHSVFYRCSFRGYQDTLYVYANRQFYRDCDIYGTVDFIFGDAVAVLQN 377
Query 643 SNIYVRKPMPNQKNTVTAQSRKDPNQNTGIVIHASRVST-----SAASGFENYLGRPWQR 479
NIYVRKPM NQ+NTVTAQ R DPN+NTGI+IH R++ + F +LGRPWQ+
Sbjct 378 CNIYVRKPMSNQQNTVTAQGRTDPNENTGIIIHNCRITAAGDLKAVQGSFRTFLGRPWQK 437
Query 478 YSRTVFMKCEIDGGIAAAGWLPWSGDFGLSTLYYGEYMNTGSGASTGGRVKWPGYHVIGS 299
YSRTV MK +DG I+ AGW PWSG+F LSTLYY E+ NTG+GASTGGRV W G+ VI S
Sbjct 438 YSRTVVMKSALDGLISPAGWFPWSGNFALSTLYYAEHANTGAGASTGGRVDWAGFRVI-S 496
Query 298 GVEAGKFSVGSFLGGDSWIPGTGVPFTSGV 209
EA KF+VG+FL G SWIPG+GVPF G+
Sbjct 497 STEAVKFTVGNFLAGGSWIPGSGVPFDEGL 526
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 9486076996
Number of extensions: 232193923
Number of successful extensions: 787420
Number of sequences better than 1e-10: 1491
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 772317
Number of HSP's successfully gapped: 1519
Length of query: 823
Length of database: 6150218869
Length adjustment: 138
Effective length of query: 685
Effective length of database: 3677385277
Effective search space: 500124397672
Effective search space used: 500124397672
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 176 (72.4 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCM3D9U016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_259
Length=1253
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002274134.2| PREDICTED: strictosidine synthase 1-like ... 252 8e-77
emb|CBI15377.3| unnamed protein product [Vitis vinifera] 252 3e-73
ref|XP_002274235.1| PREDICTED: strictosidine synthase 3-like ... 232 6e-69
emb|CAN78855.1| hypothetical protein VITISV_013355 [Vitis vin... 231 8e-69
ref|XP_002274202.2| PREDICTED: strictosidine synthase 3-like ... 231 3e-68
ALIGNMENTS
>ref|XP_002274134.2| PREDICTED: strictosidine synthase 1-like [Vitis vinifera]
Length=343
Score = 252 bits (644), Expect = 8e-77
Identities = 149/303 (49%), Positives = 201/303 (66%), Gaps = 8/303 (3%)
Frame = -3
Query 1134 FERINLPLNM-GSEAYAFDSQNVGPYTGLNDGRIVKYNGTH--FVDFATTVPNRSNKLCD 964
F ++ LP N+ G EA AFD GPYTG++DGR++KY G F DFA T P RS +CD
Sbjct 36 FTQLQLPPNVTGPEALAFDRLGGGPYTGVSDGRVLKYGGPSAGFTDFAYTTPTRSKAVCD 95
Query 963 GANANATLGRQCGRPLDLEFNNETGELYIVDLYQGLMvvgpgggvatpvaegADGMPGNA 784
G + G CGRPL + FNN TG+LYI D Y GL+VVG GG+ATPVA A+G+P
Sbjct 96 GTT-DPNSGPTCGRPLGVGFNNLTGQLYIADAYSGLLVVGSNGGLATPVATTAEGVPFRF 154
Query 783 PDAIAIDPITSEVYFTDIGTLIFTNPNMSEVLQSGDKSGKLLKYDPTTKRRSVVLTGLSG 604
+ + +D +T VYFTD + ++ ++++ +++ D SG+LLKYDP+TK+ +V++ GLSG
Sbjct 155 LNGLDVDQLTGNVYFTDASS-VYELRDITQGVENNDASGRLLKYDPSTKQVTVLIRGLSG 213
Query 603 PSGLAVSQDGSFILIAEYFACSITRYWLTGPKAHTSEHLLELPGNPDNIKRTKTGDFWVA 424
P+G AVS+DGSF+L++E+ A ++WL GPKA+TSE G PDNIK + T FWVA
Sbjct 214 PAGAAVSRDGSFVLVSEFIANRTQKFWLRGPKANTSELFFTFQGRPDNIKTSITDTFWVA 273
Query 423 VNIQKLYPQLNGFPLGQKISADGQIMQTVNFYAEYKATSISEVQEHLD-QLYVASIFTDF 247
VNI K P P GQ++ A G ++QTVNF AEY +T ISEVQ + LYV S +
Sbjct 274 VNIGKSVP--TTVPTGQRMDAHGNVLQTVNFEAEYGSTMISEVQGRGEIFLYVGSRDASY 331
Query 246 VGV 238
VGV
Sbjct 332 VGV 334
>emb|CBI15377.3| unnamed protein product [Vitis vinifera]
Length=693
Score = 252 bits (644), Expect = 3e-73
Identities = 149/303 (49%), Positives = 201/303 (66%), Gaps = 8/303 (3%)
Frame = -3
Query 1134 FERINLPLNM-GSEAYAFDSQNVGPYTGLNDGRIVKYNGTH--FVDFATTVPNRSNKLCD 964
F ++ LP N+ G EA AFD GPYTG++DGR++KY G F DFA T P RS +CD
Sbjct 36 FTQLQLPPNVTGPEALAFDRLGGGPYTGVSDGRVLKYGGPSAGFTDFAYTTPTRSKAVCD 95
Query 963 GANANATLGRQCGRPLDLEFNNETGELYIVDLYQGLMvvgpgggvatpvaegADGMPGNA 784
G + G CGRPL + FNN TG+LYI D Y GL+VVG GG+ATPVA A+G+P
Sbjct 96 GTT-DPNSGPTCGRPLGVGFNNLTGQLYIADAYSGLLVVGSNGGLATPVATTAEGVPFRF 154
Query 783 PDAIAIDPITSEVYFTDIGTLIFTNPNMSEVLQSGDKSGKLLKYDPTTKRRSVVLTGLSG 604
+ + +D +T VYFTD + ++ ++++ +++ D SG+LLKYDP+TK+ +V++ GLSG
Sbjct 155 LNGLDVDQLTGNVYFTDASS-VYELRDITQGVENNDASGRLLKYDPSTKQVTVLIRGLSG 213
Query 603 PSGLAVSQDGSFILIAEYFACSITRYWLTGPKAHTSEHLLELPGNPDNIKRTKTGDFWVA 424
P+G AVS+DGSF+L++E+ A ++WL GPKA+TSE G PDNIK + T FWVA
Sbjct 214 PAGAAVSRDGSFVLVSEFIANRTQKFWLRGPKANTSELFFTFQGRPDNIKTSITDTFWVA 273
Query 423 VNIQKLYPQLNGFPLGQKISADGQIMQTVNFYAEYKATSISEVQEHLD-QLYVASIFTDF 247
VNI K P P GQ++ A G ++QTVNF AEY +T ISEVQ + LYV S +
Sbjct 274 VNIGKSVP--TTVPTGQRMDAHGNVLQTVNFEAEYGSTMISEVQGRGEIFLYVGSRDASY 331
Query 246 VGV 238
VGV
Sbjct 332 VGV 334
Score = 205 bits (521), Expect = 8e-56
Identities = 138/310 (45%), Positives = 186/310 (60%), Gaps = 12/310 (4%)
Frame = -3
Query 1152 TQEYRNFERINLPLNMGSEAYAFDSQNVGPYTGLNDGRIVKY-NGT-HFVDFATTVPNRS 979
T + + ER+ LP G E+ AFD+ GPYTG++DGRI+KY NG+ FV+FA T N S
Sbjct 380 TYKAQFLERLPLPAT-GPESIAFDAAGGGPYTGISDGRILKYVNGSVGFVEFAITSSNSS 438
Query 978 NKLCDGANANATLGRQCGRPLDLEFNNETGELYIVDLYQGLMvvgpgggvatpvaegADG 799
+ C G N + L CGRP L F+ +TG+LYI D Y GLMVVGP GGVAT +A ADG
Sbjct 439 EEFCVG-NGSVALDFTCGRPFGLGFHYQTGDLYIADAYYGLMVVGPNGGVATQLANAADG 497
Query 798 MPGNAPDAIAIDPITSEVYFTDIGTLIFTNPNMSEVLQSGDKSGKLLKYDPTTKRRSVVL 619
+P +A+ +D T VY D + N S LQ+ D +G+L+KYDP +K +V+L
Sbjct 498 VPFGFTNALDVDTETGMVYLVDYSSQFSVN-EFSVSLQAHDMTGRLMKYDPESKELTVLL 556
Query 618 TGLSGPSGLAVSQDGSFILIAEYFACSITRYWLTGPKAHTSEHLLELPGNPDNIKRTKTG 439
GL G +G+A+S+DGSFILI E I ++WL GPKA TSE L E P NIKR + G
Sbjct 557 GGLGGAAGMAISKDGSFILITETVTKRIRKFWLQGPKATTSEILKEFTVRPANIKRNEEG 616
Query 438 DFWVAVNIQK---LYPQLNGFPLGQKISADGQIMQTVNFYAEYKATSISEVQEHLDQLYV 268
+FWVA + P P G +IS DG I++ ++ + + ISEV + ++Y+
Sbjct 617 EFWVAFLVADETGSCPSQQQSP-GLRISGDGMILEAISLDTQ---SGISEVAVYNGKMYI 672
Query 267 ASIFTDFVGV 238
S F FV V
Sbjct 673 GSPFLHFVDV 682
>ref|XP_002274235.1| PREDICTED: strictosidine synthase 3-like [Vitis vinifera]
Length=342
Score = 232 bits (591), Expect = 6e-69
Identities = 140/311 (45%), Positives = 190/311 (61%), Gaps = 13/311 (4%)
Frame = -3
Query 1137 NFERINLPLNM-GSEAYAFDSQNVGPYTGLNDGRIVKYNGTH--FVDFATTVPNRSNKLC 967
+F ++ LP N+ G + AFD GPY G+NDGRI++Y GT F DFA P RS +C
Sbjct 31 SFTQLQLPPNVTGPVSLAFDLTVGGPYAGVNDGRIIRYGGTDVGFTDFAFCTPTRSKAVC 90
Query 966 DGANANATLGRQCGRPLDLEFNNETGELYIVDLYQGLMvvgpgggvatpvaegADGMPGN 787
DG + G CGRPL L FNN ++YI D Y GL V G G +AT +A A+G+P
Sbjct 91 DGTT-DPDSGPTCGRPLGLSFNNLRNQMYIADAYSGLFVAGTNGRLATKLATSAEGVPFC 149
Query 786 APDAIAIDPITSEVYFTDIGTLIFTNPNMSEVLQSG-------DKSGKLLKYDPTTKRRS 628
+ + +DP++ VYFTD T I N+S L SG D +G+LL+YDP TK +
Sbjct 150 FLNGLDVDPLSGLVYFTDFSTTIQLR-NISRALASGNTTQFSSDATGRLLRYDPETKNVT 208
Query 627 VVLTGLSGPSGLAVSQDGSFILIAEYFACSITRYWLTGPKAHTSEHLLELPGNPDNIKRT 448
V+L GLSG +G AVS DG F+L++E+ A I ++WL GPKA T+E + G P NIKRT
Sbjct 209 VLLRGLSGAAGTAVSNDGMFVLVSEFNANRILKFWLRGPKASTAETFVSFRGRPVNIKRT 268
Query 447 KTGDFWVAVNIQKLYPQLNGFPLGQKISADGQIMQTVNFYAEY-KATSISEVQEHLDQLY 271
+G+FWVAVN+ GQ+IS G I++TV+F +Y +T I+EVQ+HL LY
Sbjct 269 ASGNFWVAVNVPNNQSPPTTILTGQRISYYGTILETVSFDDQYGGSTLITEVQQHLGALY 328
Query 270 VASIFTDFVGV 238
+ + +FVGV
Sbjct 329 IGANSANFVGV 339
>emb|CAN78855.1| hypothetical protein VITISV_013355 [Vitis vinifera]
Length=342
Score = 231 bits (590), Expect = 8e-69
Identities = 139/311 (45%), Positives = 190/311 (61%), Gaps = 13/311 (4%)
Frame = -3
Query 1137 NFERINLPLNM-GSEAYAFDSQNVGPYTGLNDGRIVKYNGTH--FVDFATTVPNRSNKLC 967
+F ++ LP N+ G + AFD GPY G+NDGRI++Y GT F DFA P RS +C
Sbjct 31 SFTQLQLPPNVTGPVSLAFDLTVGGPYAGVNDGRIIRYGGTDVGFTDFAFCTPTRSKAVC 90
Query 966 DGANANATLGRQCGRPLDLEFNNETGELYIVDLYQGLMvvgpgggvatpvaegADGMPGN 787
DG + G CGRPL L FNN ++YI D Y GL V G G +AT +A A+G+P
Sbjct 91 DGTT-DPDSGPTCGRPLGLSFNNLRNQMYIADAYSGLFVAGTNGRLATKLATSAEGVPFC 149
Query 786 APDAIAIDPITSEVYFTDIGTLIFTNPNMSEVLQSG-------DKSGKLLKYDPTTKRRS 628
+ + +DP++ VYFTD T I N+S L SG D +G+LL+YDP TK +
Sbjct 150 FLNGLDVDPLSGLVYFTDFSTTIQLR-NISRALASGNTTQFSSDATGRLLRYDPETKNVT 208
Query 627 VVLTGLSGPSGLAVSQDGSFILIAEYFACSITRYWLTGPKAHTSEHLLELPGNPDNIKRT 448
V+L GLSG +G AVS DG F+L++E+ A I ++WL GPKA T+E + G P NIKRT
Sbjct 209 VLLRGLSGAAGTAVSNDGMFVLVSEFNANRILKFWLRGPKASTAETFVSFRGRPVNIKRT 268
Query 447 KTGDFWVAVNIQKLYPQLNGFPLGQKISADGQIMQTVNFYAEY-KATSISEVQEHLDQLY 271
+G+FWVAVN+ GQ+IS G I++TV+F +Y +T I+EVQ+HL LY
Sbjct 269 ASGNFWVAVNVPNNQSPPTTILTGQRISYYGTILETVSFDDQYGGSTLITEVQQHLGALY 328
Query 270 VASIFTDFVGV 238
+ + +FVG+
Sbjct 329 IGANSANFVGI 339
>ref|XP_002274202.2| PREDICTED: strictosidine synthase 3-like [Vitis vinifera]
Length=366
Score = 231 bits (588), Expect = 3e-68
Identities = 139/316 (44%), Positives = 197/316 (62%), Gaps = 13/316 (4%)
Frame = -3
Query 1152 TQEYRN-----FERINLPLNM-GSEAYAFDSQNVGPYTGLNDGRIVKYNG--THFVDFAT 997
TQ++R F ++ LP ++ G E+ AFD + GPYTG++DGR++KY G F DFA
Sbjct 50 TQDFRVLPFTLFNKLQLPSSITGPESLAFDLKGEGPYTGVSDGRVLKYQGPAVGFTDFAV 109
Query 996 TVPNRSNKLCDGANANATLGRQCGRPLDLEFNNETGELYIVDLYQGLMvvgpgggvatpv 817
T PNR+ ++CDG+ + L CGRPL L FN TG+LY+VD Y GLMVVG GG+AT +
Sbjct 110 TSPNRTEEMCDGS-IDPALEATCGRPLGLGFNYHTGDLYMVDAYLGLMVVGSSGGIATQL 168
Query 816 aegADGMPGNAPDAIAIDPITSEVYFTDIGTLIFTNPNMSEVLQSGDKSGKLLKYDPTTK 637
A A+G+P + +D VYFT+ T F +M E++ S D +G L +YDP ++
Sbjct 169 AAAAEGIPFRFLAGLDVDQGNGMVYFTEASTR-FQLRDMQELIASNDSTGSLFRYDPQSR 227
Query 636 RRSVVLTGLSGPSGLAVSQDGSFILIAEYFACSITRYWLTGPKAHTSEHLLELPGNPDNI 457
V+L GLS G+AVS+DG F+L+AE A I R+WL GPKA+TSE +EL G P NI
Sbjct 228 EVRVLLGGLSVAVGVAVSRDGMFVLVAELTANRIRRFWLGGPKANTSEVFMELLGKPSNI 287
Query 456 KRTKTGDFWVAVNI---QKLYPQLNGFPLGQKISADGQIMQTVNFYAEYKATSISEVQEH 286
KR + G+FWVA+N P+ PLG ++S DG++++ Y+ ++ISEVQE
Sbjct 288 KRNERGEFWVAINNALGPPAPPESLVMPLGLRLSNDGRVLEVAPLVGAYQISAISEVQER 347
Query 285 LDQLYVASIFTDFVGV 238
+LYVAS+ + +
Sbjct 348 NGELYVASLVAAYASI 363
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 13440548551
Number of extensions: 296709369
Number of successful extensions: 717997
Number of sequences better than 1e-10: 548
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 715823
Number of HSP's successfully gapped: 564
Length of query: 1253
Length of database: 6150218869
Length adjustment: 143
Effective length of query: 1110
Effective length of database: 3587789857
Effective search space: 983054420818
Effective search space used: 983054420818
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCM4BW5016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_277
Length=1245
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002312331.1| predicted protein [Populus trichocarpa] >... 606 0.0
ref|XP_002278181.1| PREDICTED: ATP-citrate synthase beta chai... 595 0.0
ref|XP_003567578.1| PREDICTED: ATP-citrate synthase beta chai... 594 0.0
gb|ABR15094.1| ATP citrate lyase alpha subunit [Glycyrrhiza u... 593 0.0
ref|XP_002863984.1| ATP-citrate lyase B-2 [Arabidopsis lyrata... 592 0.0
ALIGNMENTS
>ref|XP_002312331.1| predicted protein [Populus trichocarpa]
gb|EEE89698.1| predicted protein [Populus trichocarpa]
Length=608
Score = 606 bits (1562), Expect = 0.0
Identities = 316/337 (94%), Positives = 323/337 (96%), Gaps = 0/337 (0%)
Frame = -2
Query 1244 GHAGAKSGGEMESAQAKNQALRDAGAVVPTSYEAFEGAIKETFAKLVEAGKIPAVKEVKP 1065
GHAGAKSGGEMESAQAKNQAL+DAGAVVPTSYEAFE AIKETF KLVE GKI +VKE P
Sbjct 272 GHAGAKSGGEMESAQAKNQALKDAGAVVPTSYEAFETAIKETFEKLVEEGKISSVKEFTP 331
Query 1064 PQIPEDLKTAIKSGKVRAPTHIISTISDDRGEEPTYAGVPMSSIVEQGLGVGDVISLLWF 885
PQIPEDL AIKSGKVRAPTHIISTISDDRGEEP YAGVPMSSIVEQG GVGDVISLLWF
Sbjct 332 PQIPEDLNIAIKSGKVRAPTHIISTISDDRGEEPCYAGVPMSSIVEQGYGVGDVISLLWF 391
Query 884 KRSLPHYCTRFIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA 705
KRSLP YCT FIEICIMLCADHGPCVSGAHN+IVTARAGKDLVSSLVSGLLTIGPRFGGA
Sbjct 392 KRSLPRYCTHFIEICIMLCADHGPCVSGAHNTIVTARAGKDLVSSLVSGLLTIGPRFGGA 451
Query 704 IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIKSADNRDKRVELLQLFARTNF 525
IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIK DNRDKRVELLQLFART+F
Sbjct 452 IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIKRGDNRDKRVELLQLFARTHF 511
Query 524 PSVKYMEYAVEVETYTLSKANNLVLNVDGAIgslfldllsgsGMFTKQEIDEIVGIGYLN 345
PSVKYMEYAV+VETYTLSKANNLVLNVDGAIGSLFLDLL+GSGMFTKQEIDEIVGIGYLN
Sbjct 512 PSVKYMEYAVQVETYTLSKANNLVLNVDGAIGSLFLDLLAGSGMFTKQEIDEIVGIGYLN 571
Query 344 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 234
GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLY+K
Sbjct 572 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYSK 608
>ref|XP_002278181.1| PREDICTED: ATP-citrate synthase beta chain protein 2 [Vitis vinifera]
emb|CBI22878.3| unnamed protein product [Vitis vinifera]
Length=608
Score = 595 bits (1535), Expect = 0.0
Identities = 313/337 (93%), Positives = 318/337 (94%), Gaps = 0/337 (0%)
Frame = -2
Query 1244 GHAGAKSGGEMESAQAKNQALRDAGAVVPTSYEAFEGAIKETFAKLVEAGKIPAVKEVKP 1065
GHAGAKSGGEMESAQAKNQAL +AGAVVPTSYEAFE AIKETF KL E GKI VKEV P
Sbjct 272 GHAGAKSGGEMESAQAKNQALSEAGAVVPTSYEAFETAIKETFEKLHEEGKITPVKEVAP 331
Query 1064 PQIPEDLKTAIKSGKVRAPTHIISTISDDRGEEPTYAGVPMSSIVEQGLGVGDVISLLWF 885
PQIPEDL TAIKSGKVRAPTHIISTISDDRGEEP YAGVPMSSIVEQG GVGDVISLLWF
Sbjct 332 PQIPEDLNTAIKSGKVRAPTHIISTISDDRGEEPCYAGVPMSSIVEQGYGVGDVISLLWF 391
Query 884 KRSLPHYCTRFIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA 705
KRSLP YCT FIEICIMLCADHGPCVSGAHN+IVTARAGKDLVSSLVSGLLTIGPRFGGA
Sbjct 392 KRSLPRYCTHFIEICIMLCADHGPCVSGAHNTIVTARAGKDLVSSLVSGLLTIGPRFGGA 451
Query 704 IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIKSADNRDKRVELLQLFARTNF 525
IDDAARYFKDAYDRGLT YEFVE MKKKGIRVPGIGHRIK DNRDKRVELLQ FART+F
Sbjct 452 IDDAARYFKDAYDRGLTAYEFVEDMKKKGIRVPGIGHRIKRGDNRDKRVELLQRFARTHF 511
Query 524 PSVKYMEYAVEVETYTLSKANNLVLNVDGAIgslfldllsgsGMFTKQEIDEIVGIGYLN 345
PSVKYMEYAVEVETYTLSK+NNLVLNVDGAIGSLFLDLL+GSGMFTKQEIDEIV IGYLN
Sbjct 512 PSVKYMEYAVEVETYTLSKSNNLVLNVDGAIGSLFLDLLAGSGMFTKQEIDEIVQIGYLN 571
Query 344 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 234
GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK
Sbjct 572 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 608
>ref|XP_003567578.1| PREDICTED: ATP-citrate synthase beta chain protein 1-like [Brachypodium
distachyon]
Length=608
Score = 594 bits (1531), Expect = 0.0
Identities = 307/337 (91%), Positives = 320/337 (95%), Gaps = 0/337 (0%)
Frame = -2
Query 1244 GHAGAKSGGEMESAQAKNQALRDAGAVVPTSYEAFEGAIKETFAKLVEAGKIPAVKEVKP 1065
GHAGAKSGGE+ESAQAKNQALRDAGAVVPTS+EA E IKETF KLVEAG IP V E+ P
Sbjct 272 GHAGAKSGGELESAQAKNQALRDAGAVVPTSFEALESVIKETFDKLVEAGNIPPVAEITP 331
Query 1064 PQIPEDLKTAIKSGKVRAPTHIISTISDDRGEEPTYAGVPMSSIVEQGLGVGDVISLLWF 885
P IPEDLKTAIKSGKVRAPTHIISTISDDRGEEP YAGVPMS+I+E+G GVGDVISLLWF
Sbjct 332 PPIPEDLKTAIKSGKVRAPTHIISTISDDRGEEPCYAGVPMSTIIERGYGVGDVISLLWF 391
Query 884 KRSLPHYCTRFIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA 705
KRSLP YCT+FIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA
Sbjct 392 KRSLPRYCTQFIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA 451
Query 704 IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIKSADNRDKRVELLQLFARTNF 525
IDDAARYFKDAYDRGLTPYEFVE MKKKGIRVPGIGHRIKS DNRDKRV+LLQ +A TNF
Sbjct 452 IDDAARYFKDAYDRGLTPYEFVEGMKKKGIRVPGIGHRIKSRDNRDKRVQLLQKYAHTNF 511
Query 524 PSVKYMEYAVEVETYTLSKANNLVLNVDGAIgslfldllsgsGMFTKQEIDEIVGIGYLN 345
PSVKYMEYAV+VETYTLSKANNLV+NVDGAIGSLFLDLLSGSGMF+KQEIDEI+ IGYLN
Sbjct 512 PSVKYMEYAVQVETYTLSKANNLVMNVDGAIGSLFLDLLSGSGMFSKQEIDEIIEIGYLN 571
Query 344 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 234
GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK
Sbjct 572 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 608
>gb|ABR15094.1| ATP citrate lyase alpha subunit [Glycyrrhiza uralensis]
Length=608
Score = 593 bits (1528), Expect = 0.0
Identities = 309/337 (92%), Positives = 319/337 (95%), Gaps = 0/337 (0%)
Frame = -2
Query 1244 GHAGAKSGGEMESAQAKNQALRDAGAVVPTSYEAFEGAIKETFAKLVEAGKIPAVKEVKP 1065
GHAGAKSGGEMESAQAKNQAL +AGAVVPTSYEAFE AIKETF KLVE GKIP VKE P
Sbjct 272 GHAGAKSGGEMESAQAKNQALSEAGAVVPTSYEAFEAAIKETFDKLVEEGKIPPVKEFTP 331
Query 1064 PQIPEDLKTAIKSGKVRAPTHIISTISDDRGEEPTYAGVPMSSIVEQGLGVGDVISLLWF 885
PQIPEDL TAI+SGKVRAPTHIISTISDDRGEEP YAGVPMSSI+E G GVGDVISLLWF
Sbjct 332 PQIPEDLNTAIRSGKVRAPTHIISTISDDRGEEPCYAGVPMSSIIENGYGVGDVISLLWF 391
Query 884 KRSLPHYCTRFIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA 705
KRSLP YCT+FIEICIMLCADHGPCVSGAHN+IVTARAGKDLVSSLVSGLLTIGPRFGGA
Sbjct 392 KRSLPRYCTQFIEICIMLCADHGPCVSGAHNAIVTARAGKDLVSSLVSGLLTIGPRFGGA 451
Query 704 IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIKSADNRDKRVELLQLFARTNF 525
IDDAARYFKDA DR LTPYEFVESMKKKGIRVPGIGHRIK+ DN+DKRVELLQ FART+F
Sbjct 452 IDDAARYFKDACDRSLTPYEFVESMKKKGIRVPGIGHRIKNRDNKDKRVELLQKFARTHF 511
Query 524 PSVKYMEYAVEVETYTLSKANNLVLNVDGAIgslfldllsgsGMFTKQEIDEIVGIGYLN 345
PSVKYMEYAVEVE YTL+KANNLVLNVDGAIGSLFLDLL+GSGMFTKQEIDEIV IGYLN
Sbjct 512 PSVKYMEYAVEVENYTLTKANNLVLNVDGAIGSLFLDLLAGSGMFTKQEIDEIVEIGYLN 571
Query 344 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 234
GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK
Sbjct 572 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 608
>ref|XP_002863984.1| ATP-citrate lyase B-2 [Arabidopsis lyrata subsp. lyrata]
gb|EFH40243.1| ATP-citrate lyase B-2 [Arabidopsis lyrata subsp. lyrata]
Length=608
Score = 592 bits (1527), Expect = 0.0
Identities = 308/337 (91%), Positives = 318/337 (94%), Gaps = 0/337 (0%)
Frame = -2
Query 1244 GHAGAKSGGEMESAQAKNQALRDAGAVVPTSYEAFEGAIKETFAKLVEAGKIPAVKEVKP 1065
GHAGAKSGGEMESAQAKNQAL DAGA+VPTS+EA E AIKETF KLVE GK+ +KEV P
Sbjct 272 GHAGAKSGGEMESAQAKNQALMDAGAIVPTSFEALEAAIKETFEKLVEEGKVSPIKEVTP 331
Query 1064 PQIPEDLKTAIKSGKVRAPTHIISTISDDRGEEPTYAGVPMSSIVEQGLGVGDVISLLWF 885
PQIPEDL +AIKSGKVRAPTHIISTISDDRGEEP YAGVPMSSI+EQG GVGDVISLLWF
Sbjct 332 PQIPEDLNSAIKSGKVRAPTHIISTISDDRGEEPCYAGVPMSSIIEQGYGVGDVISLLWF 391
Query 884 KRSLPHYCTRFIEICIMLCADHGPCVSGAHNSIVTARAGKDLVSSLVSGLLTIGPRFGGA 705
KRSLP YCT+FIEICIMLCADHGPCVSGAHN+IVTARAGKDLVSSLVSGLLTIGPRFGGA
Sbjct 392 KRSLPRYCTKFIEICIMLCADHGPCVSGAHNTIVTARAGKDLVSSLVSGLLTIGPRFGGA 451
Query 704 IDDAARYFKDAYDRGLTPYEFVESMKKKGIRVPGIGHRIKSADNRDKRVELLQLFARTNF 525
IDDAARYFKDA DR LTPYEFVE MKKKGIRVPGIGHRIKS DNRDKRVELLQ FAR+NF
Sbjct 452 IDDAARYFKDACDRNLTPYEFVEGMKKKGIRVPGIGHRIKSRDNRDKRVELLQKFARSNF 511
Query 524 PSVKYMEYAVEVETYTLSKANNLVLNVDGAIgslfldllsgsGMFTKQEIDEIVGIGYLN 345
PSVKYMEYAV VETYTLSKANNLVLNVDGAIGSLFLDLL+GSGMFTKQEIDEIV IGYLN
Sbjct 512 PSVKYMEYAVTVETYTLSKANNLVLNVDGAIGSLFLDLLAGSGMFTKQEIDEIVQIGYLN 571
Query 344 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 234
GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK
Sbjct 572 GLFVLARSIGLIGHTFDQKRLKQPLYRHPWEDVLYTK 608
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 13931286861
Number of extensions: 313571627
Number of successful extensions: 796081
Number of sequences better than 1e-10: 395
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 795149
Number of HSP's successfully gapped: 404
Length of query: 1245
Length of database: 6150218869
Length adjustment: 143
Effective length of query: 1102
Effective length of database: 3587789857
Effective search space: 975878841104
Effective search space used: 975878841104
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD4BETR01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_2862
Length=786
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002266452.2| PREDICTED: disease resistance response pr... 160 3e-45
ref|XP_002323339.1| predicted protein [Populus trichocarpa] >... 157 2e-44
ref|XP_002528277.1| Disease resistance response protein, puta... 157 3e-44
gb|ABK93789.1| unknown [Populus trichocarpa] 157 4e-44
ref|XP_002335799.1| predicted protein [Populus trichocarpa] >... 157 7e-44
ALIGNMENTS
>ref|XP_002266452.2| PREDICTED: disease resistance response protein 206-like [Vitis
vinifera]
emb|CAN62170.1| hypothetical protein VITISV_027159 [Vitis vinifera]
emb|CBI17737.3| unnamed protein product [Vitis vinifera]
Length=182
Score = 160 bits (404), Expect = 3e-45
Identities = 85/145 (59%), Positives = 100/145 (69%), Gaps = 3/145 (2%)
Frame = +1
Query 112 ITKLRFYFYDKPSSPNATAKLIVGTTTAPSFFGSVVMIDDALLESPNKKSKIIGRAQGLY 291
++ L FYF+D S N TA I G FG +M+DDAL E P SK++GRAQGLY
Sbjct 41 VSHLHFYFHDILSGKNPTATQIAGPKKG--HFGVTMMVDDALTEGPEPSSKLLGRAQGLY 98
Query 292 AGADQTAIGLLMVVNYVFTAGKYNGSSLSVLGRNAVFQQVREMPILGGTGAFRLARGFAL 471
A + Q LLMV+N+ F GKYNGSS+SVLGRN V VREMPI+GG+G FR ARG+AL
Sbjct 99 ALSAQQEPALLMVMNFAFMEGKYNGSSISVLGRNPVMHAVREMPIVGGSGLFRYARGYAL 158
Query 472 LSTKWFDPRGNGDAIVEYNVSVLHF 546
T WFD + GDAIVEYNVSVLHF
Sbjct 159 AHTVWFDGK-TGDAIVEYNVSVLHF 182
>ref|XP_002323339.1| predicted protein [Populus trichocarpa]
gb|EEF05100.1| predicted protein [Populus trichocarpa]
Length=178
Score = 157 bits (398), Expect = 2e-44
Identities = 83/148 (56%), Positives = 102/148 (69%), Gaps = 4/148 (3%)
Frame = +1
Query 112 ITKLRFYFYDKPSSPNATAKLIVG---TTTAPSFFGSVVMIDDALLESPNKKSKIIGRAQ 282
++ L FYF+D S N TA I T T+ + FG V MIDD L +P SK++GRAQ
Sbjct 32 LSHLHFYFHDIVSGKNPTAVRIARADMTNTSSTGFGMVAMIDDPLTMTPELSSKLVGRAQ 91
Query 283 GLYAGADQTAIGLLMVVNYVFTAGKYNGSSLSVLGRNAVFQQVREMPILGGTGAFRLARG 462
G YA A Q +GLLM +N+VF GK+NGS+LSVLGRN+VF VREMPI+GG+G FR ARG
Sbjct 92 GFYASASQNDVGLLMTMNFVFMEGKFNGSTLSVLGRNSVFSTVREMPIVGGSGLFRFARG 151
Query 463 FALLSTKWFDPRGNGDAIVEYNVSVLHF 546
+A ST FD R GDA+VEYNV V H+
Sbjct 152 YAQASTHMFD-RTTGDAVVEYNVYVFHY 178
>ref|XP_002528277.1| Disease resistance response protein, putative [Ricinus communis]
gb|EEF34115.1| Disease resistance response protein, putative [Ricinus communis]
Length=186
Score = 157 bits (398), Expect = 3e-44
Identities = 75/147 (51%), Positives = 104/147 (71%), Gaps = 4/147 (3%)
Frame = +1
Query 112 ITKLRFYFYDKPSSPNATAKLIV---GTTTAPSFFGSVVMIDDALLESPNKKSKIIGRAQ 282
+T L+FYF+D S N TA + T +P+ FG+++M DD L E P+ KSK++GRAQ
Sbjct 41 VTNLQFYFHDIVSGKNRTAIRVAQSADTEKSPTLFGALIMADDPLTEGPDPKSKLVGRAQ 100
Query 283 GLYAGADQTAIGLLMVVNYVFTAGKYNGSSLSVLGRNAVFQQVREMPILGGTGAFRLARG 462
GLY A Q + L+M +NY FT+G YNGSS+S+LG+N+ VRE+P++GGTG FR+ARG
Sbjct 101 GLYGSAGQNELCLIMAMNYAFTSGAYNGSSISILGKNSAMSPVRELPVVGGTGVFRMARG 160
Query 463 FALLSTKWFDPRGNGDAIVEYNVSVLH 543
+A+ T WFD GDAIV YN++V+H
Sbjct 161 YAIARTHWFDIT-TGDAIVGYNLTVVH 186
>gb|ABK93789.1| unknown [Populus trichocarpa]
Length=194
Score = 157 bits (398), Expect = 4e-44
Identities = 83/148 (56%), Positives = 102/148 (69%), Gaps = 4/148 (3%)
Frame = +1
Query 112 ITKLRFYFYDKPSSPNATAKLIVG---TTTAPSFFGSVVMIDDALLESPNKKSKIIGRAQ 282
++ L FYF+D S N TA I T T+ + FG V MIDD L +P SK++GRAQ
Sbjct 48 LSHLHFYFHDIVSGKNPTAVRIARADMTNTSSTGFGMVAMIDDPLTMTPELSSKLVGRAQ 107
Query 283 GLYAGADQTAIGLLMVVNYVFTAGKYNGSSLSVLGRNAVFQQVREMPILGGTGAFRLARG 462
G YA A Q +GLLM +N+VF GK+NGS+LSVLGRN+VF VREMPI+GG+G FR ARG
Sbjct 108 GFYASASQNDVGLLMTMNFVFMEGKFNGSTLSVLGRNSVFSTVREMPIVGGSGLFRFARG 167
Query 463 FALLSTKWFDPRGNGDAIVEYNVSVLHF 546
+A ST FD R GDA+VEYNV V H+
Sbjct 168 YAQASTHMFD-RTTGDAVVEYNVYVFHY 194
>ref|XP_002335799.1| predicted protein [Populus trichocarpa]
gb|EEE73321.1| predicted protein [Populus trichocarpa]
Length=193
Score = 157 bits (396), Expect = 7e-44
Identities = 81/147 (55%), Positives = 104/147 (71%), Gaps = 3/147 (2%)
Frame = +1
Query 112 ITKLRFYFYDKPSSPNATAKLIVG--TTTAPSFFGSVVMIDDALLESPNKKSKIIGRAQG 285
+T R Y++D S+PN TA IV + T+ + FGS+ MIDD L E P SK+IGRAQG
Sbjct 48 LTHFRVYWHDIYSAPNPTAMPIVRAPSNTSATLFGSISMIDDPLTEKPELSSKLIGRAQG 107
Query 286 LYAGADQTAIGLLMVVNYVFTAGKYNGSSLSVLGRNAVFQQVREMPILGGTGAFRLARGF 465
Y A Q LLM +N+VF GKYNGS++S+LGRN VF +VREMP++GG+G FR ARG+
Sbjct 108 FYGSAGQEETALLMAMNFVFLQGKYNGSTISILGRNHVFSKVREMPVIGGSGLFRFARGY 167
Query 466 ALLSTKWFDPRGNGDAIVEYNVSVLHF 546
A ST FD + +GDA+VEYNV VLH+
Sbjct 168 AQASTHSFDLK-SGDAVVEYNVYVLHY 193
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 392749991
Number of extensions: 8530628
Number of successful extensions: 26277
Number of sequences better than 1e-10: 16
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 26234
Number of HSP's successfully gapped: 16
Length of query: 786
Length of database: 6150218869
Length adjustment: 138
Effective length of query: 648
Effective length of database: 3677385277
Effective search space: 455995774348
Effective search space used: 455995774348
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 175 (72.0 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD4D4R501N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_3088
Length=768
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002317421.1| predicted protein [Populus trichocarpa] >... 96.7 3e-21
ref|XP_002273122.2| PREDICTED: uncharacterized protein LOC100... 95.9 3e-21
emb|CAN72178.1| hypothetical protein VITISV_012478 [Vitis vin... 95.9 5e-21
ref|XP_002522583.1| conserved hypothetical protein [Ricinus c... 95.5 7e-21
ref|XP_002305745.1| predicted protein [Populus trichocarpa] >... 95.1 1e-20
ALIGNMENTS
>ref|XP_002317421.1| predicted protein [Populus trichocarpa]
gb|ABK94387.1| unknown [Populus trichocarpa]
gb|EEE98033.1| predicted protein [Populus trichocarpa]
Length=177
Score = 96.7 bits (239), Expect = 3e-21
Identities = 45/49 (92%), Positives = 48/49 (98%), Gaps = 0/49 (0%)
Frame = -2
Query 767 WGSLDALIGRLRAAYEENGGSQETNPFGNGSIRVYLREVKDCQAKARGI 621
WGSLDALIGRLRAAYEE+GGS ETNPFGNG+IRVYLREVK+CQAKARGI
Sbjct 104 WGSLDALIGRLRAAYEEHGGSAETNPFGNGAIRVYLREVKECQAKARGI 152
>ref|XP_002273122.2| PREDICTED: uncharacterized protein LOC100254926 [Vitis vinifera]
emb|CBI30686.3| unnamed protein product [Vitis vinifera]
Length=149
Score = 95.9 bits (237), Expect = 3e-21
Identities = 44/49 (90%), Positives = 48/49 (98%), Gaps = 0/49 (0%)
Frame = -2
Query 767 WGSLDALIGRLRAAYEENGGSQETNPFGNGSIRVYLREVKDCQAKARGI 621
WGSLDALIGRLRAAYEE+GGS ETNPFGNG+IRVYLREV+DCQ+KARGI
Sbjct 76 WGSLDALIGRLRAAYEEHGGSPETNPFGNGAIRVYLREVRDCQSKARGI 124
>emb|CAN72178.1| hypothetical protein VITISV_012478 [Vitis vinifera]
Length=175
Score = 95.9 bits (237), Expect = 5e-21
Identities = 44/49 (90%), Positives = 48/49 (98%), Gaps = 0/49 (0%)
Frame = -2
Query 767 WGSLDALIGRLRAAYEENGGSQETNPFGNGSIRVYLREVKDCQAKARGI 621
WGSLDALIGRLRAAYEE+GGS ETNPFGNG+IRVYLREV+DCQ+KARGI
Sbjct 102 WGSLDALIGRLRAAYEEHGGSPETNPFGNGAIRVYLREVRDCQSKARGI 150
>ref|XP_002522583.1| conserved hypothetical protein [Ricinus communis]
gb|EEF39883.1| conserved hypothetical protein [Ricinus communis]
Length=180
Score = 95.5 bits (236), Expect = 7e-21
Identities = 44/49 (90%), Positives = 48/49 (98%), Gaps = 0/49 (0%)
Frame = -2
Query 767 WGSLDALIGRLRAAYEENGGSQETNPFGNGSIRVYLREVKDCQAKARGI 621
WGSLDALIGRLRAAYEE+GGS ETNPFGNG+IRVYLREV++CQAKARGI
Sbjct 105 WGSLDALIGRLRAAYEEHGGSPETNPFGNGAIRVYLREVRECQAKARGI 153
>ref|XP_002305745.1| predicted protein [Populus trichocarpa]
gb|ABK93578.1| unknown [Populus trichocarpa]
gb|EEE86256.1| predicted protein [Populus trichocarpa]
Length=177
Score = 95.1 bits (235), Expect = 1e-20
Identities = 44/49 (90%), Positives = 48/49 (98%), Gaps = 0/49 (0%)
Frame = -2
Query 767 WGSLDALIGRLRAAYEENGGSQETNPFGNGSIRVYLREVKDCQAKARGI 621
WGSLDALIGRLRAA+EE+GGS ETNPFGNG+IRVYLREVK+CQAKARGI
Sbjct 104 WGSLDALIGRLRAAFEEHGGSAETNPFGNGAIRVYLREVKECQAKARGI 152
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 305746453
Number of extensions: 5236553
Number of successful extensions: 10219
Number of sequences better than 1e-10: 11
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 10216
Number of HSP's successfully gapped: 11
Length of query: 768
Length of database: 6150218869
Length adjustment: 137
Effective length of query: 631
Effective length of database: 3695304361
Effective search space: 439741218959
Effective search space used: 439741218959
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 175 (72.0 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD5MSRZ01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_3187
Length=762
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002271428.2| PREDICTED: pathogenesis-related protein S... 149 3e-41
ref|XP_002328585.1| predicted protein [Populus trichocarpa] >... 147 1e-40
gb|ACB12048.1| pathogenesis-related protein [Rehmannia glutin... 145 7e-40
gb|ABR10301.1| pathogen-related protein STH-2 [Salvia miltior... 139 1e-37
ref|XP_002271360.1| PREDICTED: major allergen Pru ar 1 [Vitis... 137 6e-37
ALIGNMENTS
>ref|XP_002271428.2| PREDICTED: pathogenesis-related protein STH-2 [Vitis vinifera]
emb|CBI22930.3| unnamed protein product [Vitis vinifera]
Length=160
Score = 149 bits (375), Expect = 3e-41
Identities = 72/160 (45%), Positives = 111/160 (69%), Gaps = 2/160 (1%)
Frame = -2
Query 704 MAVTKHSQEITVSVSPKRMIKALVTDASTFLPKIMSGTIQSIVVLSGNGGPGTIIQTNFS 525
M VT +QE VSP RM KALV D+ +P+++ +++SI + G+GG G+I QTNFS
Sbjct 1 MGVTTFTQEFVTPVSPARMFKALVVDSHILVPRLVPESVKSIEFVEGDGGAGSITQTNFS 60
Query 524 DVAGAPVPTAKHRIDALDAEKGTTKFTMIEGAYLGEKIESVVYDVKFEEAGNGGCTIKVT 345
+ K++I+A+D EK ++T+IEG LG+++ES+VY++KFEE+G+GGC K
Sbjct 61 --GDSDCEYLKYKINAVDKEKLECRYTLIEGGVLGDQLESIVYEMKFEESGDGGCICKTR 118
Query 344 NEYNSKGDAALKDEDIKEIVDRAKGFYIAAEAYLIANPNE 225
+EY++KG+ +K+E I+E ++A G Y EAYL+ANP+E
Sbjct 119 SEYHTKGEFEIKEESIREGKEKAMGVYKLVEAYLLANPDE 158
>ref|XP_002328585.1| predicted protein [Populus trichocarpa]
gb|EEE76932.1| predicted protein [Populus trichocarpa]
Length=160
Score = 147 bits (371), Expect = 1e-40
Identities = 71/159 (45%), Positives = 104/159 (65%), Gaps = 2/159 (1%)
Frame = -2
Query 704 MAVTKHSQEITVSVSPKRMIKALVTDASTFLPKIMSGTIQSIVVLSGNGGPGTIIQTNFS 525
M V ++QE T +SP RM KAL+ D++ +PK++ ++S+ ++ G+GG G+I Q NF+
Sbjct 1 MGVASYTQEFTCPISPARMFKALILDSNNLIPKLLPQIVKSVDLIHGDGGAGSIEQVNFT 60
Query 524 DVAGAPVPTAKHRIDALDAEKGTTKFTMIEGAYLGEKIESVVYDVKFEEAGNGGCTIKVT 345
+ G + KHRID LD K+TMIEG LGEK+ES+ Y+V+FE +GGC K+T
Sbjct 61 E--GTDIKYVKHRIDELDRVNLVCKYTMIEGDSLGEKLESIAYEVRFEVGSDGGCDCKMT 118
Query 344 NEYNSKGDAALKDEDIKEIVDRAKGFYIAAEAYLIANPN 228
+ Y GD LK+E+IK D+A+G Y EAYL+ NP+
Sbjct 119 SSYLMLGDFTLKEEEIKAGQDKARGIYKVVEAYLLENPH 157
>gb|ACB12048.1| pathogenesis-related protein [Rehmannia glutinosa]
Length=154
Score = 145 bits (365), Expect = 7e-40
Identities = 79/162 (49%), Positives = 112/162 (69%), Gaps = 8/162 (5%)
Frame = -2
Query 704 MAVTKHSQEITVSVSPKRMIKALVTDASTFLPKIMSGTIQSIVVLSGNGGPGTIIQTNFS 525
M +TKH QE+ + VS KRM KALVT++ + +P + I+SI +L G+G GTI +TN +
Sbjct 1 MGITKHIQELKLRVSAKRMFKALVTESHS-IP--LPDAIKSIEILHGDGSAGTIRKTNLA 57
Query 524 DVAGAPVPTAKHRIDALDAEKGTTKFTMIEGAYLGEKIESVVYDVKFEEAGNGGCTIKVT 345
D G+ V K RI+A+D + +K+T+IEG LG+KIES+ Y+ KFE++ +GGC K+
Sbjct 58 D--GSYV---KIRIEAVDIDNQVSKYTVIEGPMLGDKIESIHYEQKFEDSSDGGCVAKIV 112
Query 344 NEYNSKGDAALKDEDIKEIVDRAKGFYIAAEAYLIANPNECA 219
EY++KGD LK+E +K I D+A GFY +E YL ANPN CA
Sbjct 113 CEYHTKGDIQLKEEGVKAINDQALGFYTLSEEYLHANPNVCA 154
>gb|ABR10301.1| pathogen-related protein STH-2 [Salvia miltiorrhiza]
Length=160
Score = 139 bits (350), Expect = 1e-37
Identities = 72/163 (44%), Positives = 107/163 (66%), Gaps = 4/163 (2%)
Frame = -2
Query 704 MAVTKHSQEITVSVSPKRMIKALVTDASTFLPKIMSGTIQSIVVLSGNG-GPGTIIQTNF 528
M V QE+ +S R+ KALVT++ +PK + +I+SI ++ G+G PG I QTNF
Sbjct 1 MGVKSFFQEMKTKISSSRLFKALVTESPEVVPKFTT-SIKSIELIQGSGYAPGAIFQTNF 59
Query 527 SDVAGAPVPTAKHRIDALDAEKGTTKFTMIEGAYLGEKIESVVYDVKFEEAGNGGCTIKV 348
+ GA K R+D +D EK + K+T+IEG LG+K+E + YD+KFE+ +GGC +KV
Sbjct 60 PE--GAHFKYMKCRVDEIDHEKHSIKYTLIEGDMLGDKLEKICYDMKFEDTEDGGCVVKV 117
Query 347 TNEYNSKGDAALKDEDIKEIVDRAKGFYIAAEAYLIANPNECA 219
T+EY++KG L DED+K +++ G Y + E YL+ANP+ CA
Sbjct 118 TSEYHTKGGYELADEDLKGAKEQSLGMYKSCEDYLLANPHVCA 160
>ref|XP_002271360.1| PREDICTED: major allergen Pru ar 1 [Vitis vinifera]
emb|CAN79555.1| hypothetical protein VITISV_025729 [Vitis vinifera]
emb|CBI22942.3| unnamed protein product [Vitis vinifera]
Length=161
Score = 137 bits (346), Expect = 6e-37
Identities = 75/159 (47%), Positives = 101/159 (64%), Gaps = 3/159 (2%)
Frame = -2
Query 704 MAVTKHSQEITVSVSPKRMIKALVTDASTFLPKIMSGTIQSIVVLSGNGGPGTIIQTNFS 525
M VT+ SQ+ V+P RM KAL+ D+ PK+M +I+SI L G G G+I Q NF+
Sbjct 1 MGVTRLSQKYATHVTPGRMFKALILDSHNLCPKLMFSSIKSIEFLEGEGEVGSIKQINFT 60
Query 524 DVAGAPVPTAKHRIDALDAEKGTTKFTMIE-GAYLGEKIESVVYDVKFEEAGNGGCTIKV 348
+ + P+ KHRIDALD EK T +TM E A + +KIE + YDVKFE G GGC +
Sbjct 61 EAS--PLTYMKHRIDALDKEKLTCTYTMFETDALIMDKIEYITYDVKFEPYGFGGCVCNL 118
Query 347 TNEYNSKGDAALKDEDIKEIVDRAKGFYIAAEAYLIANP 231
T+ Y +KGD +++EDI+ DRA G Y EAYL+A+P
Sbjct 119 TSVYKTKGDVEIREEDIEHGKDRAIGMYEVLEAYLMAHP 157
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 375474024
Number of extensions: 8001644
Number of successful extensions: 23298
Number of sequences better than 1e-10: 14
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 23215
Number of HSP's successfully gapped: 14
Length of query: 762
Length of database: 6150218869
Length adjustment: 137
Effective length of query: 625
Effective length of database: 3695304361
Effective search space: 432350610237
Effective search space used: 432350610237
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 175 (72.0 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCM5CG001N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_328
Length=1209
Score E
Sequences producing significant alignments: (Bits) Value
gb|ACZ51443.1| peroxidase protein [Mikania micrantha] 459 6e-158
dbj|BAA77389.1| peroxidase 3 [Scutellaria baicalensis] 456 8e-157
ref|XP_002268259.2| PREDICTED: cationic peroxidase 1-like [Vi... 457 1e-156
gb|ACJ11762.1| class III peroxidase [Gossypium hirsutum] 434 3e-148
sp|P22195.2|PER1_ARAHY RecName: Full=Cationic peroxidase 1; A... 430 1e-146
ALIGNMENTS
>gb|ACZ51443.1| peroxidase protein [Mikania micrantha]
Length=321
Score = 459 bits (1180), Expect = 6e-158
Identities = 225/297 (76%), Positives = 258/297 (87%), Gaps = 3/297 (1%)
Frame = +3
Query 60 GQLSPNFYSTTCPNLLSIISSAVNTALSTEQRMGASLLRLHFHDCFVNGCDASVLLDDTA 239
GQLS NFY+T+CPN SIISSAVN+A+S E RMGASLLRLHFHDCFVNGCDASVLLDDT
Sbjct 27 GQLSANFYATSCPNFSSIISSAVNSAVSNEARMGASLLRLHFHDCFVNGCDASVLLDDTT 86
Query 240 NFTGEKTAGPNNNSLRGFEVIDNIKAQVETSCSGIVSCADILAVAARDSVVVLGGPSWTV 419
NFTGEKTAGPNNNSLRGF+VID IK+Q+E+SC G+VSCAD+LA AARDSVV LGGPSW +
Sbjct 87 NFTGEKTAGPNNNSLRGFDVIDTIKSQLESSCPGVVSCADLLATAARDSVVALGGPSWNL 146
Query 420 PLGRRDSTSASLSAANNQIPAPTLNLNALITSFSNQGFTAQELVALSGAHTIGQARCTTF 599
GRRDS +ASLSAAN+ IPAPTLNL+ LITSFSN GFTA E+VALSG+HTIGQARCT F
Sbjct 147 AFGRRDSITASLSAANSNIPAPTLNLSGLITSFSNLGFTANEMVALSGSHTIGQARCTVF 206
Query 600 RTRLYNEANINASYATTLQAGCPSIGPTGDNNLSGLD-SDPTAFNNQYFTDLINLRGLLH 776
R R+YNE NIN+S+AT+L+A CPS G GDNNLS LD PT+F+N YFT+L+N GLLH
Sbjct 207 RARIYNENNINSSFATSLRANCPSSG--GDNNLSPLDVVSPTSFDNTYFTNLLNQNGLLH 264
Query 777 SDQELFNNGSTDAQVTAYSTNSTSFFTDFANAMIKMSNLSPLTGTNGQIRTNCSRTN 947
SDQELFN GSTDAQV YS+N+ +F TDFAN M+KMSNL+PLTG++GQ+RTNC RTN
Sbjct 265 SDQELFNGGSTDAQVRTYSSNAATFSTDFANGMVKMSNLNPLTGSSGQVRTNCRRTN 321
>dbj|BAA77389.1| peroxidase 3 [Scutellaria baicalensis]
Length=318
Score = 456 bits (1172), Expect = 8e-157
Identities = 226/299 (76%), Positives = 256/299 (86%), Gaps = 3/299 (1%)
Frame = +3
Query 54 SHGQLSPNFYSTTCPNLLSIISSAVNTALSTEQRMGASLLRLHFHDCFVNGCDASVLLDD 233
S+ QLS NFY+TTCPNLL+II +AVN+A+S++ RMGASLLRLHFHDCFVNGCDASVLLDD
Sbjct 22 SNAQLSANFYNTTCPNLLTIIRNAVNSAVSSDTRMGASLLRLHFHDCFVNGCDASVLLDD 81
Query 234 TANFTGEKTAGPNNNSLRGFEVIDNIKAQVETSCSGIVSCADILAVAARDSVVVLGGPSW 413
FTGEKTAGPN NSLRGF+VIDNIK VE SC IVSC+DIL+VAARD VV +GGPSW
Sbjct 82 RTGFTGEKTAGPNANSLRGFDVIDNIKTLVEGSCPNIVSCSDILSVAARDGVVAVGGPSW 141
Query 414 TVPLGRRDSTSASLSAANNQIPAPTLNLNALITSFSNQGFTAQELVALSGAHTIGQARCT 593
V LGRRDST+ASL+AAN QIP P LNLNALITSFSN+GFTA+E+VALSG+HTIGQARCT
Sbjct 142 AVALGRRDSTTASLNAANTQIPGPGLNLNALITSFSNKGFTAREMVALSGSHTIGQARCT 201
Query 594 TFRTRLYNEANINASYATTLQAGCPSIGPTGDNNLSGLDS-DPTAFNNQYFTDLINLRGL 770
TFR R+YN+ NIN ++AT L+A CP G GDNNL+ LD+ P FNN Y+ +LI LRGL
Sbjct 202 TFRGRIYNDTNINGAFATGLRANCPRSG--GDNNLAPLDNVSPARFNNDYYRNLIGLRGL 259
Query 771 LHSDQELFNNGSTDAQVTAYSTNSTSFFTDFANAMIKMSNLSPLTGTNGQIRTNCSRTN 947
LHSDQELFNNG+ DAQV AYSTNS +FF DFANAM+KMSNLSPLTGTNGQIR NC RTN
Sbjct 260 LHSDQELFNNGTADAQVRAYSTNSAAFFNDFANAMVKMSNLSPLTGTNGQIRRNCRRTN 318
>ref|XP_002268259.2| PREDICTED: cationic peroxidase 1-like [Vitis vinifera]
Length=376
Score = 457 bits (1177), Expect = 1e-156
Identities = 224/296 (76%), Positives = 261/296 (88%), Gaps = 3/296 (1%)
Frame = +3
Query 63 QLSPNFYSTTCPNLLSIISSAVNTALSTEQRMGASLLRLHFHDCFVNGCDASVLLDDTAN 242
QL+ N+YS++CPN LSII SAVNTA++ E RMGASLLRLHFHDCFVNGCDAS+LLDDT+N
Sbjct 83 QLTTNYYSSSCPNALSIIKSAVNTAVNNEARMGASLLRLHFHDCFVNGCDASILLDDTSN 142
Query 243 FTGEKTAGPNNNSLRGFEVIDNIKAQVETSCSGIVSCADILAVAARDSVVVLGGPSWTVP 422
FTGEKTA PN NS+RGF+VID IK+QVE+SC G+VSCADILAV ARDSVV LGGPSWTV
Sbjct 143 FTGEKTAVPNANSVRGFDVIDTIKSQVESSCPGVVSCADILAVVARDSVVALGGPSWTVR 202
Query 423 LGRRDSTSASLSAANNQIPAPTLNLNALITSFSNQGFTAQELVALSGAHTIGQARCTTFR 602
LGRRDST+ASLS AN+ IPAPTLNL+ LI+SFSN+GF+A E+VALSG+HTIGQARCT FR
Sbjct 203 LGRRDSTTASLSTANSDIPAPTLNLSGLISSFSNKGFSANEMVALSGSHTIGQARCTNFR 262
Query 603 TRLYNEANINASYATTLQAGCPSIGPTGDNNLSGLDS-DPTAFNNQYFTDLINLRGLLHS 779
RLYNE NI+AS+ ++LQA CPS G GDNNLS LD+ PT F+N YFT+L+N +GLLHS
Sbjct 263 DRLYNETNIDASFQSSLQANCPSSG--GDNNLSPLDTKSPTTFDNAYFTNLVNNKGLLHS 320
Query 780 DQELFNNGSTDAQVTAYSTNSTSFFTDFANAMIKMSNLSPLTGTNGQIRTNCSRTN 947
DQ+LFN GSTD+QVT YST ST+FFTDFANA++KM NLSPLTGT+GQIRTNC +TN
Sbjct 321 DQQLFNGGSTDSQVTTYSTKSTTFFTDFANAIVKMGNLSPLTGTSGQIRTNCRKTN 376
>gb|ACJ11762.1| class III peroxidase [Gossypium hirsutum]
Length=323
Score = 434 bits (1116), Expect = 3e-148
Identities = 217/296 (73%), Positives = 253/296 (85%), Gaps = 3/296 (1%)
Frame = +3
Query 63 QLSPNFYSTTCPNLLSIISSAVNTALSTEQRMGASLLRLHFHDCFVNGCDASVLLDDTAN 242
QLS FYSTTCP LS I SAVN+A+S E RMGASL RLHFHDCFVNGCD S+LLDDTAN
Sbjct 30 QLSSTFYSTTCPRALSTIKSAVNSAVSNEARMGASLPRLHFHDCFVNGCDGSILLDDTAN 89
Query 243 FTGEKTAGPNNNSLRGFEVIDNIKAQVETSCSGIVSCADILAVAARDSVVVLGGPSWTVP 422
TGEKTA PN+NS RGFEVID IK+QVE+ C G+VSCADI+AVAARDSVV LGGPSW V
Sbjct 90 MTGEKTAVPNSNSARGFEVIDTIKSQVESLCPGVVSCADIVAVAARDSVVALGGPSWIVL 149
Query 423 LGRRDSTSASLSAANNQIPAPTLNLNALITSFSNQGFTAQELVALSGAHTIGQARCTTFR 602
LGRRDST+ASLSAAN+ IPAPTLNL+ LIT+FSN+GFTA+E+VALSG+HTIGQARCTTFR
Sbjct 150 LGRRDSTTASLSAANSNIPAPTLNLSGLITAFSNKGFTAKEMVALSGSHTIGQARCTTFR 209
Query 603 TRLYNEANINASYATTLQAGCPSIGPTGDNNLSGLD-SDPTAFNNQYFTDLINLRGLLHS 779
TR+YNE NI++++AT+L+A CPS G GDN+LS LD + T+F+N YF +L +GLLHS
Sbjct 210 TRIYNETNIDSTFATSLRANCPSNG--GDNSLSPLDTTSSTSFDNAYFKNLQGQKGLLHS 267
Query 780 DQELFNNGSTDAQVTAYSTNSTSFFTDFANAMIKMSNLSPLTGTNGQIRTNCSRTN 947
DQ+LF+ GSTD+QV AYS+N SF TDFANAM+KM NLSPLTGT+GQIRTNC + N
Sbjct 268 DQQLFSGGSTDSQVNAYSSNLGSFTTDFANAMVKMGNLSPLTGTSGQIRTNCRKAN 323
>sp|P22195.2|PER1_ARAHY RecName: Full=Cationic peroxidase 1; AltName: Full=PNPC1; Flags:
Precursor
gb|AAB06183.1| cationic peroxidase [Arachis hypogaea]
Length=316
Score = 430 bits (1105), Expect = 1e-146
Identities = 213/296 (72%), Positives = 249/296 (84%), Gaps = 3/296 (1%)
Frame = +3
Query 63 QLSPNFYSTTCPNLLSIISSAVNTALSTEQRMGASLLRLHFHDCFVNGCDASVLLDDTAN 242
QLS NFY+T CPN LS I SAVN+A++ E RMGASLLRLHFHDCFV GCDASVLLDDT+N
Sbjct 23 QLSSNFYATKCPNALSTIKSAVNSAVAKEARMGASLLRLHFHDCFVQGCDASVLLDDTSN 82
Query 243 FTGEKTAGPNNNSLRGFEVIDNIKAQVETSCSGIVSCADILAVAARDSVVVLGGPSWTVP 422
FTGEKTAGPN NS+RGFEVID IK+QVE+ C G+VSCADILAVAARDSVV LGG SW V
Sbjct 83 FTGEKTAGPNANSIRGFEVIDTIKSQVESLCPGVVSCADILAVAARDSVVALGGASWNVL 142
Query 423 LGRRDSTSASLSAANNQIPAPTLNLNALITSFSNQGFTAQELVALSGAHTIGQARCTTFR 602
LGRRDST+ASLS+AN+ +PAP NL+ LI++FSN+GFT +ELV LSGAHTIGQA+CT FR
Sbjct 143 LGRRDSTTASLSSANSDLPAPFFNLSGLISAFSNKGFTTKELVTLSGAHTIGQAQCTAFR 202
Query 603 TRLYNEANINASYATTLQAGCPSIGPTGDNNLSGLD-SDPTAFNNQYFTDLINLRGLLHS 779
TR+YNE+NI+ +YA +LQA CPS+G GD NLS D + P F+N Y+ +L N +GLLHS
Sbjct 203 TRIYNESNIDPTYAKSLQANCPSVG--GDTNLSPFDVTTPNKFDNAYYINLRNKKGLLHS 260
Query 780 DQELFNNGSTDAQVTAYSTNSTSFFTDFANAMIKMSNLSPLTGTNGQIRTNCSRTN 947
DQ+LFN STD+QVTAYS N+ +F TDF NAMIKM NLSPLTGT+GQIRTNC +TN
Sbjct 261 DQQLFNGVSTDSQVTAYSNNAATFNTDFGNAMIKMGNLSPLTGTSGQIRTNCRKTN 316
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 638584556
Number of extensions: 13696293
Number of successful extensions: 34605
Number of sequences better than 1e-10: 100
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 34287
Number of HSP's successfully gapped: 100
Length of query: 1209
Length of database: 6150218869
Length adjustment: 143
Effective length of query: 1066
Effective length of database: 3587789857
Effective search space: 932825362820
Effective search space used: 932825362820
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD6R91601S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_3586
Length=736
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002304640.1| AP2/ERF domain-containing transcription f... 145 5e-39
dbj|BAJ34121.1| unnamed protein product [Thellungiella haloph... 143 2e-38
ref|NP_196837.1| ethylene-responsive transcription factor ERF... 142 2e-38
gb|ACO55953.1| RAP2-like protein [Juglans nigra] 141 9e-38
gb|ADN33698.1| AP2 domain transcription factor RAP2 [Cucumis ... 141 2e-37
ALIGNMENTS
>ref|XP_002304640.1| AP2/ERF domain-containing transcription factor [Populus trichocarpa]
gb|EEE79619.1| AP2/ERF domain-containing transcription factor [Populus trichocarpa]
Length=234
Score = 145 bits (365), Expect = 5e-39
Identities = 98/200 (49%), Positives = 124/200 (62%), Gaps = 17/200 (9%)
Frame = -1
Query 733 DNNSTIISSLTTNHGASDSAAALPPPERVEDARKDQGVKK-HYRGVRQRPWGKWAAEIRD 557
DNN T + S A D + P++ + + + ++ HYRGVRQRPWGKWAAEIRD
Sbjct 16 DNNPTSVQSTPY---ALDQSVVKDEPDQSQPVQDQENTRRRHYRGVRQRPWGKWAAEIRD 72
Query 556 PKKAARVWLGTFdtaedaalayddaALKFKGSKAKLNFPERVQGNTTQLHYPTSSAHQHM 377
PKKAARVWLGTFDTAEDAA+AYD AALKFKG+KAKLNFPERVQG T +Y S ++
Sbjct 73 PKKAARVWLGTFDTAEDAAVAYDKAALKFKGTKAKLNFPERVQGRTEFGYYMGSGTSTNV 132
Query 376 ------------PHTTQPGPLLSSANAFPGLQQYAQLLSSNDDADIPYFTSALYSSTADY 233
P P P + + +P L QYAQ+LSSN DA+ PY+TS L++
Sbjct 133 LTEQSPRPVAPPPPPPPPPPSSFAPDTYPDLLQYAQILSSN-DANFPYYTSNLFNQQPFA 191
Query 232 QTASASYLTLQHQDYQSRFT 173
S+S+L+ Q Q RF+
Sbjct 192 PHYSSSFLSQQQQQDLMRFS 211
>dbj|BAJ34121.1| unnamed protein product [Thellungiella halophila]
Length=231
Score = 143 bits (361), Expect = 2e-38
Identities = 98/220 (45%), Positives = 129/220 (59%), Gaps = 30/220 (14%)
Frame = -1
Query 718 IISSLTT--NHGASDSAAALPPPERVE-------DARKDQGVKKHYRGVRQRPWGKWAAE 566
+ISSL+ + SD + PP + E +DQ ++HYRGVRQRPWGKWAAE
Sbjct 1 MISSLSRAIEYPTSDGQESSDPPVKQELDKSDQLQQDQDQPRRRHYRGVRQRPWGKWAAE 60
Query 565 IRDPKKAARVWLGTFdtaedaalayddaALKFKGSKAKLNFPERVQGNTTQLHYPTSSAH 386
IRDPKKAARVWLGTF+TAE+AALAYD AALKFKG+KAKLNFPERVQG +T + + +
Sbjct 61 IRDPKKAARVWLGTFETAEEAALAYDRAALKFKGTKAKLNFPERVQGPSTTTYVASQTGF 120
Query 385 QHMPH----------------TTQPGPLLSSANAFPGLQQYAQLLSSNDDADIPYFTSAL 254
H P TT P N + QYAQLL+SN+D D+ Y+TS+L
Sbjct 121 DHAPRGGSELMNSPPPRLGPSTTTTAPTSWPTNYNQDILQYAQLLTSNNDVDLSYYTSSL 180
Query 253 YSS-----TADYQTASASYLTLQHQDYQSRFTGDHKHPGS 149
+S + ++S+++ + Q Q Q + H+ S
Sbjct 181 FSQQQQPFSTPSSSSSSTFTSQQTQQQQQQQQQQHEEEKS 220
>ref|NP_196837.1| ethylene-responsive transcription factor ERF113 [Arabidopsis
thaliana]
sp|Q9LYU3.1|EF113_ARATH RecName: Full=Ethylene-responsive transcription factor ERF113
emb|CAB86640.1| putative protein [Arabidopsis thaliana]
gb|AAS47615.1| At5g13330 [Arabidopsis thaliana]
gb|AAS76737.1| At5g13330 [Arabidopsis thaliana]
gb|AAT44928.1| putative AP2/EREBP transcription factor [Arabidopsis thaliana]
gb|AED91881.1| ethylene-responsive transcription factor ERF113 [Arabidopsis
thaliana]
Length=212
Score = 142 bits (359), Expect = 2e-38
Identities = 90/174 (52%), Positives = 117/174 (67%), Gaps = 10/174 (6%)
Frame = -1
Query 634 KDQGVKKHYRGVRQRPWGKWAAEIRDPKKAARVWLGTFdtaedaalayddaALKFKGSKA 455
+DQ ++HYRGVRQRPWGKWAAEIRDPKKAARVWLGTF+TAE+AALAYD AALKFKG+KA
Sbjct 31 QDQPRRRHYRGVRQRPWGKWAAEIRDPKKAARVWLGTFETAEEAALAYDRAALKFKGTKA 90
Query 454 KLNFPERVQGNTTQL---HYPTSSAHQHMPHTTQPGPLLSSANAFP-----GLQQYAQLL 299
KLNFPERVQG TT H P + +PGP ++ ++P + QYAQLL
Sbjct 91 KLNFPERVQGPTTTTTISHAPRGVSESMNSPPPRPGPPSTTTTSWPMTYNQDILQYAQLL 150
Query 298 SSNDDADIPYFTSALYSS--TADYQTASASYLTLQHQDYQSRFTGDHKHPGSGF 143
+SN++ D+ Y+TS L+S + ++S+S T Q Q Q + + + G+
Sbjct 151 TSNNEVDLSYYTSTLFSQPFSTPSSSSSSSQQTQQQQLQQQQQQREEEEKNYGY 204
>gb|ACO55953.1| RAP2-like protein [Juglans nigra]
Length=226
Score = 141 bits (356), Expect = 9e-38
Identities = 103/218 (47%), Positives = 131/218 (60%), Gaps = 27/218 (12%)
Frame = -1
Query 736 EDNNSTIISSLT-TNHGASDSAAALP----PPERVEDARKDQGVKKHYRGVRQRPWGKWA 572
ED S+++ S T H A +P P + E+AR+ +HYRGVRQRPWGKWA
Sbjct 16 EDQQSSLVQSTPLTIHEAGPVKEEVPDSSQPVQDQENARR-----RHYRGVRQRPWGKWA 70
Query 571 AEIRDPKKAARVWLGTFdtaedaalayddaALKFKGSKAKLNFPERVQGNTTQLHYPTSS 392
AEIRDPKKAARVWLGTF+TAEDAALAYD AAL+FKG+KAKLNFPERVQG T++ YP+
Sbjct 71 AEIRDPKKAARVWLGTFETAEDAALAYDRAALRFKGTKAKLNFPERVQG-TSEFGYPSGF 129
Query 391 AHQHM-----------PHTTQPGPLLSSANAFPGLQQYAQLLSSNDDADIPYFTSALY-- 251
+ + P P L + FPGL QYAQLLSS+ D + PY+TS+L+
Sbjct 130 GNTSVSQSAVPVQNPRPVVNPPPSTLPQESTFPGLYQYAQLLSSS-DVEFPYYTSSLFNQ 188
Query 250 -SSTADYQT-ASASYLTLQHQDYQSRFTGDHKHPGSGF 143
S + + + +S S H +Q R + G F
Sbjct 189 ESFASQFSSFSSPSSAASSHHPHQERQDQEEDDKGRQF 226
>gb|ADN33698.1| AP2 domain transcription factor RAP2 [Cucumis melo subsp. melo]
Length=252
Score = 141 bits (355), Expect = 2e-37
Identities = 94/169 (56%), Positives = 114/169 (67%), Gaps = 11/169 (7%)
Frame = -1
Query 625 GVKKHYRGVRQRPWGKWAAEIRDPKKAARVWLGTFdtaedaalayddaALKFKGSKAKLN 446
G K+HYRGVRQRPWGKWAAEIRDPKKAARVWLGTFDTAE AA+AYD+AAL+FKG+KAKLN
Sbjct 83 GRKRHYRGVRQRPWGKWAAEIRDPKKAARVWLGTFDTAEAAAIAYDNAALRFKGTKAKLN 142
Query 445 FPERVQGNTTQLHY---PTSSAHQHMPHTTQPG----PLLSSANAFPGLQQYAQLLSSND 287
FPERVQ N + + P+ +A + P P LS FPGL QYAQLLSS+
Sbjct 143 FPERVQANPAEFGFLSPPSIAAPPPPTAVSSPSVSLPPPLSHEEVFPGLHQYAQLLSSS- 201
Query 286 DADIPYFTSALYSSTADYQ---TASASYLTLQHQDYQSRFTGDHKHPGS 149
DAD PY++S+L + +S+S + Q Q+ Q R+ D GS
Sbjct 202 DADFPYYSSSLLFNQQQSHYPFFSSSSSSSQQQQEDQDRYGEDFGTGGS 250
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 381165112
Number of extensions: 8230657
Number of successful extensions: 30957
Number of sequences better than 1e-10: 36
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 30783
Number of HSP's successfully gapped: 36
Length of query: 736
Length of database: 6150218869
Length adjustment: 137
Effective length of query: 599
Effective length of database: 3695304361
Effective search space: 399092870988
Effective search space used: 399092870988
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 175 (72.0 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD6WZSR01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_3754
Length=725
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002276040.1| PREDICTED: uncharacterized protein LOC100... 71.6 1e-11
emb|CBI24383.3| unnamed protein product [Vitis vinifera] 71.6 2e-11
gb|ACF83094.1| unknown [Zea mays] 70.9 2e-11
gb|ACF84316.1| unknown [Zea mays] 70.9 2e-11
ref|NP_001149939.1| RNA binding protein [Zea mays] >gb|ACG372... 70.9 2e-11
ALIGNMENTS
>ref|XP_002276040.1| PREDICTED: uncharacterized protein LOC100263294 [Vitis vinifera]
Length=457
Score = 71.6 bits (174), Expect = 1e-11
Identities = 41/106 (39%), Positives = 57/106 (54%), Gaps = 10/106 (9%)
Frame = -1
Query 725 RFGEIEDVPIGNDPVTQKFKGFAVITYKSVEGYNKAMAEPNKVFENCQLHCKKFQENLNK 546
+FGEIED P+G D T KF+GFA+I +K+ EG +A+ EP K FE+C+L C +
Sbjct 241 KFGEIEDGPLGFDKATGKFRGFAIIVFKTAEGMKRALEEPIKTFESCKLQCSRKVAKTTP 300
Query 545 KNINNGPSQTGPASMNP---------IGVNYGGFGTNMNAGMGLVG 435
TG A++ P +G+N G G N+N LVG
Sbjct 301 VQQQAVAGSTG-ATLPPSNYQLQAYQMGLNQGLIGQNVNPQGVLVG 345
>emb|CBI24383.3| unnamed protein product [Vitis vinifera]
Length=583
Score = 71.6 bits (174), Expect = 2e-11
Identities = 41/106 (39%), Positives = 57/106 (54%), Gaps = 10/106 (9%)
Frame = -1
Query 725 RFGEIEDVPIGNDPVTQKFKGFAVITYKSVEGYNKAMAEPNKVFENCQLHCKKFQENLNK 546
+FGEIED P+G D T KF+GFA+I +K+ EG +A+ EP K FE+C+L C +
Sbjct 327 KFGEIEDGPLGFDKATGKFRGFAIIVFKTAEGMKRALEEPIKTFESCKLQCSRKVAKTTP 386
Query 545 KNINNGPSQTGPASMNP---------IGVNYGGFGTNMNAGMGLVG 435
TG A++ P +G+N G G N+N LVG
Sbjct 387 VQQQAVAGSTG-ATLPPSNYQLQAYQMGLNQGLIGQNVNPQGVLVG 431
>gb|ACF83094.1| unknown [Zea mays]
Length=379
Score = 70.9 bits (172), Expect = 2e-11
Identities = 40/97 (41%), Positives = 54/97 (56%), Gaps = 1/97 (1%)
Frame = -1
Query 725 RFGEIEDVPIGNDPVTQKFKGFAVITYKSVEGYNKAMAEPNKVFENCQLHCKKFQENLNK 546
RFGEIE+ P+G D VT K KGFA+ YK++E KA+ EP+K FE LHC+K + K
Sbjct 253 RFGEIEEGPLGLDKVTGKPKGFALFVYKTIESAKKALEEPHKHFEGVMLHCQKAIDG-PK 311
Query 545 KNINNGPSQTGPASMNPIGVNYGGFGTNMNAGMGLVG 435
N G A + G + G+G ++ G VG
Sbjct 312 PNKLGGAGGFFGAGASSGGKGFAGYGATSHSLPGTVG 348
>gb|ACF84316.1| unknown [Zea mays]
Length=410
Score = 70.9 bits (172), Expect = 2e-11
Identities = 40/97 (41%), Positives = 54/97 (56%), Gaps = 1/97 (1%)
Frame = -1
Query 725 RFGEIEDVPIGNDPVTQKFKGFAVITYKSVEGYNKAMAEPNKVFENCQLHCKKFQENLNK 546
RFGEIE+ P+G D VT K KGFA+ YK++E KA+ EP+K FE LHC+K + K
Sbjct 209 RFGEIEEGPLGLDKVTGKPKGFALFVYKTIESAKKALEEPHKHFEGVMLHCQKAIDG-PK 267
Query 545 KNINNGPSQTGPASMNPIGVNYGGFGTNMNAGMGLVG 435
N G A + G + G+G ++ G VG
Sbjct 268 PNKLGGAGGFFGAGASSGGKGFAGYGATSHSLPGTVG 304
>ref|NP_001149939.1| RNA binding protein [Zea mays]
gb|ACG37277.1| RNA binding protein [Zea mays]
Length=455
Score = 70.9 bits (172), Expect = 2e-11
Identities = 40/97 (41%), Positives = 54/97 (56%), Gaps = 1/97 (1%)
Frame = -1
Query 725 RFGEIEDVPIGNDPVTQKFKGFAVITYKSVEGYNKAMAEPNKVFENCQLHCKKFQENLNK 546
RFGEIE+ P+G D VT K KGFA+ YK++E KA+ EP+K FE LHC+K + K
Sbjct 254 RFGEIEEGPLGLDKVTGKPKGFALFVYKTIESAKKALEEPHKHFEGVMLHCQKAIDG-PK 312
Query 545 KNINNGPSQTGPASMNPIGVNYGGFGTNMNAGMGLVG 435
N G A + G + G+G ++ G VG
Sbjct 313 PNKLGGAGGFFGAGASSGGKGFAGYGATSHSLPGTVG 349
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 369861218
Number of extensions: 8110291
Number of successful extensions: 20203
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 20162
Number of HSP's successfully gapped: 0
Length of query: 725
Length of database: 6150218869
Length adjustment: 137
Effective length of query: 588
Effective length of database: 3695304361
Effective search space: 384311653544
Effective search space used: 384311653544
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 175 (72.0 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD7VCNZ01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_4049
Length=708
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002448594.1| hypothetical protein SORBIDRAFT_06g029810... 261 2e-82
emb|CAH67668.1| H0315F07.6 [Oryza sativa Indica Group] >dbj|B... 238 3e-76
gb|EEE61761.1| hypothetical protein OsJ_16303 [Oryza sativa J... 238 7e-73
gb|EEC78090.1| hypothetical protein OsI_17570 [Oryza sativa I... 238 8e-73
ref|XP_003579353.1| PREDICTED: putative serine/threonine-prot... 230 7e-66
ALIGNMENTS
>ref|XP_002448594.1| hypothetical protein SORBIDRAFT_06g029810 [Sorghum bicolor]
gb|EES12922.1| hypothetical protein SORBIDRAFT_06g029810 [Sorghum bicolor]
Length=383
Score = 261 bits (667), Expect = 2e-82
Identities = 130/146 (89%), Positives = 135/146 (92%), Gaps = 2/146 (1%)
Frame = -3
Query 706 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSLLDDEWSMNLLGYAWTLWKEGSVS 527
YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSS +DDEWSMNLLGYAWTLWKEGSVS
Sbjct 237 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSFVDDEWSMNLLGYAWTLWKEGSVS 296
Query 526 ELIDPLMGATYAYDEACRCVQVGLLCVQEVPAERPAMPLVLRMLSGDAAIPSPKQAAFFV 347
ELIDPLMG TY YDE CRC+QVGLLCVQE+PAERP M +VLRMLSGD IPSPKQAAFFV
Sbjct 297 ELIDPLMGTTYTYDEVCRCIQVGLLCVQELPAERPTMSMVLRMLSGDVTIPSPKQAAFFV 356
Query 346 GRAPRLPPADDSSNTESGNQLTCTDL 269
GRAPRL PADD+S TESGNQLT TDL
Sbjct 357 GRAPRL-PADDNS-TESGNQLTYTDL 380
>emb|CAH67668.1| H0315F07.6 [Oryza sativa Indica Group]
dbj|BAG92610.1| unnamed protein product [Oryza sativa Japonica Group]
Length=166
Score = 238 bits (607), Expect = 3e-76
Identities = 114/146 (78%), Positives = 127/146 (87%), Gaps = 5/146 (3%)
Frame = -3
Query 706 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSLLDDEWSMNLLGYAWTLWKEGSVS 527
YISPEYAMEGKFS+KSD+FSFGVL+LEIVSGRRNSS +D+EWSMNLLGYAWTLWKEGSVS
Sbjct 23 YISPEYAMEGKFSEKSDIFSFGVLILEIVSGRRNSSFVDEEWSMNLLGYAWTLWKEGSVS 82
Query 526 ELIDPLMGATYAYDEACRCVQVGLLCVQEVPAERPAMPLVLRMLSGDAAIPSPKQAAFFV 347
ELIDPLMG +YDE CRC+QVGLLCVQE+P +RP+MPLVLRMLSGD +P+PKQAAFFV
Sbjct 83 ELIDPLMGTICSYDEVCRCIQVGLLCVQELPGDRPSMPLVLRMLSGDVTLPAPKQAAFFV 142
Query 346 GRAPRLPPADDSSNTESGNQLTCTDL 269
GR P D +NT SGNQLT T L
Sbjct 143 GRVPL-----DDNNTGSGNQLTYTQL 163
>gb|EEE61761.1| hypothetical protein OsJ_16303 [Oryza sativa Japonica Group]
Length=425
Score = 238 bits (607), Expect = 7e-73
Identities = 114/146 (78%), Positives = 127/146 (87%), Gaps = 5/146 (3%)
Frame = -3
Query 706 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSLLDDEWSMNLLGYAWTLWKEGSVS 527
YISPEYAMEGKFS+KSD+FSFGVL+LEIVSGRRNSS +D+EWSMNLLGYAWTLWKEGSVS
Sbjct 282 YISPEYAMEGKFSEKSDIFSFGVLILEIVSGRRNSSFVDEEWSMNLLGYAWTLWKEGSVS 341
Query 526 ELIDPLMGATYAYDEACRCVQVGLLCVQEVPAERPAMPLVLRMLSGDAAIPSPKQAAFFV 347
ELIDPLMG +YDE CRC+QVGLLCVQE+P +RP+MPLVLRMLSGD +P+PKQAAFFV
Sbjct 342 ELIDPLMGTICSYDEVCRCIQVGLLCVQELPGDRPSMPLVLRMLSGDVTLPAPKQAAFFV 401
Query 346 GRAPRLPPADDSSNTESGNQLTCTDL 269
GR P D +NT SGNQLT T L
Sbjct 402 GRVPL-----DDNNTGSGNQLTYTQL 422
>gb|EEC78090.1| hypothetical protein OsI_17570 [Oryza sativa Indica Group]
Length=431
Score = 238 bits (607), Expect = 8e-73
Identities = 114/146 (78%), Positives = 127/146 (87%), Gaps = 5/146 (3%)
Frame = -3
Query 706 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSLLDDEWSMNLLGYAWTLWKEGSVS 527
YISPEYAMEGKFS+KSD+FSFGVL+LEIVSGRRNSS +D+EWSMNLLGYAWTLWKEGSVS
Sbjct 288 YISPEYAMEGKFSEKSDIFSFGVLILEIVSGRRNSSFVDEEWSMNLLGYAWTLWKEGSVS 347
Query 526 ELIDPLMGATYAYDEACRCVQVGLLCVQEVPAERPAMPLVLRMLSGDAAIPSPKQAAFFV 347
ELIDPLMG +YDE CRC+QVGLLCVQE+P +RP+MPLVLRMLSGD +P+PKQAAFFV
Sbjct 348 ELIDPLMGTICSYDEVCRCIQVGLLCVQELPGDRPSMPLVLRMLSGDVTLPAPKQAAFFV 407
Query 346 GRAPRLPPADDSSNTESGNQLTCTDL 269
GR P D +NT SGNQLT T L
Sbjct 408 GRVPL-----DDNNTGSGNQLTYTQL 428
>ref|XP_003579353.1| PREDICTED: putative serine/threonine-protein kinase receptor-like
[Brachypodium distachyon]
Length=1217
Score = 230 bits (586), Expect = 7e-66
Identities = 112/146 (77%), Positives = 124/146 (85%), Gaps = 5/146 (3%)
Frame = -3
Query 706 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSLLDDEWSMNLLGYAWTLWKEGSVS 527
YISPEYAMEGKFS+KSDVFSFGVLVLEIV GRRNSS +DDEWSMNL+G+AWTLWKE S
Sbjct 233 YISPEYAMEGKFSEKSDVFSFGVLVLEIVCGRRNSSFIDDEWSMNLVGHAWTLWKEDRTS 292
Query 526 ELIDPLMGATYAYDEACRCVQVGLLCVQEVPAERPAMPLVLRMLSGDAAIPSPKQAAFFV 347
ELID LMG Y+ DE CRC+QVGLLCVQE+P ERPAMPLVLRMLSGD A+P+PK+AAFFV
Sbjct 293 ELIDALMGTAYSQDEVCRCIQVGLLCVQELPGERPAMPLVLRMLSGDVALPAPKRAAFFV 352
Query 346 GRAPRLPPADDSSNTESGNQLTCTDL 269
GRAP D +TESGN LT T+L
Sbjct 353 GRAP-----VDDKDTESGNHLTYTEL 373
Score = 93.6 bits (231), Expect = 2e-18
Identities = 62/154 (40%), Positives = 82/154 (53%), Gaps = 25/154 (16%)
Frame = -3
Query 706 YISPEYAMEGKFSDKSDVFSFGVLVLEIVSGRRNSSLLDDEWSMNLLGYAWTLWKEGSVS 527
Y +PEYA +G+ + K DV+SFGV++LE +SG RN + L+ +AW LW++
Sbjct 1074 YAAPEYARQGEMTLKCDVYSFGVILLETLSGERNGGM------QRLISHAWELWEQNRAM 1127
Query 526 ELID----PLM---GATYAYDEACRCVQVGLLCVQEVPAERPAMPLVLRMLSGDAAIPSP 368
EL+D PL E RCVQ+GLLCVQE P +RPAM V+ ML+ A SP
Sbjct 1128 ELLDKATVPLPDPESEPQLLSELKRCVQIGLLCVQETPCDRPAMSAVVAMLTSTA---SP 1184
Query 367 KQAAFFVGRAPRLPP---ADDSSNTESGNQLTCT 275
+ R RL A SS TE+ CT
Sbjct 1185 ------IDRPRRLLDSGWATSSSRTETDLLSPCT 1212
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 411689089
Number of extensions: 9536440
Number of successful extensions: 30672
Number of sequences better than 1e-10: 227
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 30055
Number of HSP's successfully gapped: 229
Length of query: 708
Length of database: 6150218869
Length adjustment: 136
Effective length of query: 572
Effective length of database: 3713223445
Effective search space: 371322344500
Effective search space used: 371322344500
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 175 (72.0 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD7ZJ0F01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_4574
Length=680
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002273122.2| PREDICTED: uncharacterized protein LOC100... 121 3e-31
emb|CAN72178.1| hypothetical protein VITISV_012478 [Vitis vin... 120 2e-30
ref|XP_003531259.1| PREDICTED: uncharacterized protein LOC100... 119 3e-30
ref|XP_002522583.1| conserved hypothetical protein [Ricinus c... 119 5e-30
ref|XP_003542999.1| PREDICTED: uncharacterized protein LOC100... 116 8e-29
ALIGNMENTS
>ref|XP_002273122.2| PREDICTED: uncharacterized protein LOC100254926 [Vitis vinifera]
emb|CBI30686.3| unnamed protein product [Vitis vinifera]
Length=149
Score = 121 bits (304), Expect = 3e-31
Identities = 61/80 (76%), Positives = 69/80 (86%), Gaps = 4/80 (5%)
Frame = -1
Query 680 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGSIRVYLKEVKDCQAKARGIPYKKNKKRK 501
QAWGSLDALIGRLRAAYEEHGGSPETNPFGNG+IRVYL+EV+DCQ+KARGIPYKK KK+K
Sbjct 74 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGAIRVYLREVRDCQSKARGIPYKKKKKKK 133
Query 500 ISHTSIKGHDEVIKIHKQAA 441
IK ++E K KQ+A
Sbjct 134 ---NQIKPNNEA-KSSKQSA 149
>emb|CAN72178.1| hypothetical protein VITISV_012478 [Vitis vinifera]
Length=175
Score = 120 bits (300), Expect = 2e-30
Identities = 60/79 (76%), Positives = 68/79 (86%), Gaps = 4/79 (5%)
Frame = -1
Query 680 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGSIRVYLKEVKDCQAKARGIPYKKNKKRK 501
QAWGSLDALIGRLRAAYEEHGGSPETNPFGNG+IRVYL+EV+DCQ+KARGIPYKK KK+K
Sbjct 100 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGAIRVYLREVRDCQSKARGIPYKKKKKKK 159
Query 500 ISHTSIKGHDEVIKIHKQA 444
IK ++E K KQ+
Sbjct 160 ---NQIKPNNEA-KSSKQS 174
>ref|XP_003531259.1| PREDICTED: uncharacterized protein LOC100810655 [Glycine max]
Length=167
Score = 119 bits (299), Expect = 3e-30
Identities = 58/80 (73%), Positives = 67/80 (84%), Gaps = 2/80 (3%)
Frame = -1
Query 680 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGSIRVYLKEVKDCQAKARGIPYKKNKKRK 501
QAWGSLDALIGRLRAAYEEHGGSPETNPFG G+IRVYL+EVK+CQAKARGIPYKK KK++
Sbjct 90 QAWGSLDALIGRLRAAYEEHGGSPETNPFGGGAIRVYLREVKECQAKARGIPYKKKKKKR 149
Query 500 ISHTSIKGHDEVIKIHKQAA 441
+ +KG I +QA+
Sbjct 150 --NPILKGTQRAKDIEQQAS 167
>ref|XP_002522583.1| conserved hypothetical protein [Ricinus communis]
gb|EEF39883.1| conserved hypothetical protein [Ricinus communis]
Length=180
Score = 119 bits (298), Expect = 5e-30
Identities = 54/60 (90%), Positives = 59/60 (98%), Gaps = 0/60 (0%)
Frame = -1
Query 680 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGSIRVYLKEVKDCQAKARGIPYKKNKKRK 501
QAWGSLDALIGRLRAAYEEHGGSPETNPFGNG+IRVYL+EV++CQAKARGIPYKK KK+K
Sbjct 103 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGAIRVYLREVRECQAKARGIPYKKKKKKK 162
>ref|XP_003542999.1| PREDICTED: uncharacterized protein LOC100778886 [Glycine max]
Length=181
Score = 116 bits (290), Expect = 8e-29
Identities = 58/80 (73%), Positives = 65/80 (81%), Gaps = 3/80 (4%)
Frame = -1
Query 680 QAWGSLDALIGRLRAAYEEHGGSPETNPFGNGSIRVYLKEVKDCQAKARGIPYKKNKKRK 501
QAWGSLDALIGRLRAAYEE GGSPETNPFG+G+IRVYL+EVK+CQAKARGIPY K KK+K
Sbjct 104 QAWGSLDALIGRLRAAYEERGGSPETNPFGSGAIRVYLREVKECQAKARGIPYIKKKKKK 163
Query 500 ISHTSIKGHDEVIKIHKQAA 441
+KG + K KQ A
Sbjct 164 ---NQLKGPHDAPKSFKQLA 180
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 287505189
Number of extensions: 5394626
Number of successful extensions: 12073
Number of sequences better than 1e-10: 14
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 12071
Number of HSP's successfully gapped: 14
Length of query: 680
Length of database: 6150218869
Length adjustment: 136
Effective length of query: 544
Effective length of database: 3713223445
Effective search space: 334190110050
Effective search space used: 334190110050
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 174 (71.6 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCND41W012
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_463
Length=1143
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002513669.1| conserved hypothetical protein [Ricinus c... 216 3e-64
ref|XP_003634095.1| PREDICTED: uncharacterized protein LOC100... 214 1e-63
emb|CAN78886.1| hypothetical protein VITISV_002549 [Vitis vin... 213 3e-63
ref|XP_002318206.1| predicted protein [Populus trichocarpa] >... 208 5e-62
ref|XP_002275480.1| PREDICTED: uncharacterized protein LOC100... 209 9e-62
ALIGNMENTS
>ref|XP_002513669.1| conserved hypothetical protein [Ricinus communis]
gb|EEF49072.1| conserved hypothetical protein [Ricinus communis]
Length=265
Score = 216 bits (550), Expect = 3e-64
Identities = 102/153 (67%), Positives = 121/153 (79%), Gaps = 1/153 (1%)
Frame = +2
Query 164 MGPRYEVEITITSAKDLKNVNWRYGKLRPYAVVWVDPKSKCSTRVDEEGDTTPLWDDKLI 343
M RYEVE++I+SAKDLKNVNWR+G LRPYAVVW+DP SK STRVDEEGDT P WD L+
Sbjct 1 MASRYEVEVSISSAKDLKNVNWRHGLLRPYAVVWIDPNSKWSTRVDEEGDTCPFWDQILV 60
Query 344 IPFD-SPIEDSTLHIDIVQADAAEDTKPLIGSGKLHLRDIVDDVGLGILAEKKLDLKRPS 520
IP PIED TL+ID+V A+A EDTKPLIGS +L L +++DDVG+G + L LKRPS
Sbjct 61 IPLPPGPIEDHTLYIDVVHANAEEDTKPLIGSARLKLSEVLDDVGIGQRLNRILQLKRPS 120
Query 521 GRPQGKLDVKVSVREPRYRAPDPYYALPYGVPP 619
GRPQGK+DV V++R+P Y P YYA PYGVPP
Sbjct 121 GRPQGKMDVNVTIRQPSYHPPPAYYAPPYGVPP 153
>ref|XP_003634095.1| PREDICTED: uncharacterized protein LOC100852496 [Vitis vinifera]
emb|CAN62171.1| hypothetical protein VITISV_014432 [Vitis vinifera]
Length=268
Score = 214 bits (546), Expect = 1e-63
Identities = 97/154 (63%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
Frame = +2
Query 164 MGPRYEVEITITSAKDLKNVNWRYGKLRPYAVVWVDPKSKCSTRVDEEGDTTPLWDDKLI 343
M PR+E+++ I+SAKDLKNVNWR+G L+PYAVVWV+P +KCST+VD +GDT P W++ L
Sbjct 1 MAPRFELKLKISSAKDLKNVNWRHGSLKPYAVVWVEPNAKCSTKVDHDGDTFPSWNETLH 60
Query 344 IPFDSPIEDSTLHIDIV--QADAAEDTKPLIGSGKLHLRDIVDDVGLGILAEKKLDLKRP 517
IP S I+DS L++DIV ++D +DTKP++GS +L LRD+VDDVG G+ A + L+L+RP
Sbjct 61 IPVPSSIDDSALYLDIVHVKSDDEDDTKPMVGSARLFLRDVVDDVGFGVEAFRTLELRRP 120
Query 518 SGRPQGKLDVKVSVREPRYRAPDPYYALPYGVPP 619
SGRPQGK+DV+VSVR+ RYRAP+PYY PYGVPP
Sbjct 121 SGRPQGKVDVEVSVRDLRYRAPEPYYTAPYGVPP 154
>emb|CAN78886.1| hypothetical protein VITISV_002549 [Vitis vinifera]
Length=268
Score = 213 bits (543), Expect = 3e-63
Identities = 96/154 (62%), Positives = 128/154 (83%), Gaps = 2/154 (1%)
Frame = +2
Query 164 MGPRYEVEITITSAKDLKNVNWRYGKLRPYAVVWVDPKSKCSTRVDEEGDTTPLWDDKLI 343
M PR+E+++ I+SAKDLKNVNWR+G L+PYAVVWV+P +KCST+VD +GDT P W++ L
Sbjct 1 MAPRFELKLKISSAKDLKNVNWRHGSLKPYAVVWVEPNAKCSTKVDHDGDTFPSWNETLH 60
Query 344 IPFDSPIEDSTLHIDIV--QADAAEDTKPLIGSGKLHLRDIVDDVGLGILAEKKLDLKRP 517
IP S I+DS L++DIV ++D +DTKP++GS +L LRD+VDDVG G+ A + L+L+RP
Sbjct 61 IPVPSSIDDSALYLDIVHVKSDDEDDTKPMVGSARLFLRDVVDDVGFGVEAFRTLELRRP 120
Query 518 SGRPQGKLDVKVSVREPRYRAPDPYYALPYGVPP 619
SGRPQGK+DV+VSV++ RYRAP+PYY PYGVPP
Sbjct 121 SGRPQGKVDVEVSVKDLRYRAPEPYYTAPYGVPP 154
>ref|XP_002318206.1| predicted protein [Populus trichocarpa]
gb|EEE96426.1| predicted protein [Populus trichocarpa]
Length=216
Score = 208 bits (530), Expect = 5e-62
Identities = 102/152 (67%), Positives = 118/152 (78%), Gaps = 1/152 (1%)
Frame = +2
Query 164 MGPRYEVEITITSAKDLKNVNWRYGKLRPYAVVWVDPKSKCSTRVDEEGDTTPLWDDKLI 343
M RYE E+TI+SAK LKNVNWR+G L+PYAVVWVD KCST+VD+EGDT+P WD L+
Sbjct 1 MASRYEFEVTISSAKYLKNVNWRHGSLKPYAVVWVDSNYKCSTQVDDEGDTSPFWDQTLV 60
Query 344 IPFDSP-IEDSTLHIDIVQADAAEDTKPLIGSGKLHLRDIVDDVGLGILAEKKLDLKRPS 520
IP S IED TLHIDIV A + E TKPLIGS KL L D++DDV +G A + L LKRPS
Sbjct 61 IPLPSGRIEDHTLHIDIVHAGSEEGTKPLIGSAKLKLIDVLDDVEIGERATRALQLKRPS 120
Query 521 GRPQGKLDVKVSVREPRYRAPDPYYALPYGVP 616
GRPQGKLDVKV++R+PRYRAPD Y PYG P
Sbjct 121 GRPQGKLDVKVTIRDPRYRAPDAYREPPYGQP 152
>ref|XP_002275480.1| PREDICTED: uncharacterized protein LOC100242355 [Vitis vinifera]
Length=268
Score = 209 bits (533), Expect = 9e-62
Identities = 95/154 (62%), Positives = 127/154 (82%), Gaps = 2/154 (1%)
Frame = +2
Query 164 MGPRYEVEITITSAKDLKNVNWRYGKLRPYAVVWVDPKSKCSTRVDEEGDTTPLWDDKLI 343
M PR+E+++ I+SAKDLKNVNW +G L+PYAVVWV+P +KCST+VD +GDT P W++ L
Sbjct 1 MAPRFELKLKISSAKDLKNVNWCHGSLKPYAVVWVEPNAKCSTKVDHDGDTFPSWNETLH 60
Query 344 IPFDSPIEDSTLHIDIV--QADAAEDTKPLIGSGKLHLRDIVDDVGLGILAEKKLDLKRP 517
IP S I+DS L++DIV ++D +DTKP++GS +L LRD+VDDVG G+ A + L+L+RP
Sbjct 61 IPVPSSIDDSALYLDIVHVKSDDEDDTKPMVGSARLFLRDVVDDVGFGVEAFRTLELRRP 120
Query 518 SGRPQGKLDVKVSVREPRYRAPDPYYALPYGVPP 619
SGRPQGK+DV+VSVR+ R+RAP+PYY PYGVPP
Sbjct 121 SGRPQGKVDVEVSVRDLRHRAPEPYYTAPYGVPP 154
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 9983923121
Number of extensions: 200630892
Number of successful extensions: 671249
Number of sequences better than 1e-10: 62
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 670163
Number of HSP's successfully gapped: 62
Length of query: 1143
Length of database: 6150218869
Length adjustment: 142
Effective length of query: 1001
Effective length of database: 3605708941
Effective search space: 861764436899
Effective search space used: 861764436899
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD921ZD01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_4716
Length=673
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003550308.1| PREDICTED: U-box domain-containing protei... 214 2e-64
gb|AAM64910.1| unknown [Arabidopsis thaliana] 213 3e-64
ref|NP_566136.1| armadillo/beta-catenin-like repeat-containin... 213 3e-64
ref|XP_002510099.1| E3 ubiquitin ligase PUB14, putative [Rici... 212 9e-64
ref|XP_003557013.1| PREDICTED: U-box domain-containing protei... 208 8e-63
ALIGNMENTS
>ref|XP_003550308.1| PREDICTED: U-box domain-containing protein 4-like [Glycine max]
Length=352
Score = 214 bits (544), Expect = 2e-64
Identities = 110/137 (80%), Positives = 126/137 (92%), Gaps = 0/137 (0%)
Frame = -1
Query 673 LCSVKENKVRAVQAGIMRPLVEMMSDLGSNMVDKSAFVLSLLVPLTEARAALVEEGGIPV 494
LC+VKENK+RAV+AGIM+ LVE+M+D SNMVDKSA+V+S+LV + EAR ALVEEGG+PV
Sbjct 210 LCTVKENKIRAVKAGIMKVLVELMADFESNMVDKSAYVVSVLVAVPEARVALVEEGGVPV 269
Query 493 LVEIVEVGTQRQKEIAVAILLQLCEDCVSYRTMVAREGAIPPLVALSQSGTSRAKQKAEA 314
LVEIVEVGTQRQKEIAV ILLQ+CED V+YRTMVAREGAIPPLVALSQSGT+RAKQKAE
Sbjct 270 LVEIVEVGTQRQKEIAVVILLQVCEDSVTYRTMVAREGAIPPLVALSQSGTNRAKQKAEK 329
Query 313 LIEVLRQPRSTNGAANN 263
LIE+LRQPRS NGAA +
Sbjct 330 LIELLRQPRSGNGAARS 346
>gb|AAM64910.1| unknown [Arabidopsis thaliana]
Length=355
Score = 213 bits (542), Expect = 3e-64
Identities = 109/138 (79%), Positives = 128/138 (93%), Gaps = 1/138 (1%)
Frame = -1
Query 673 LCSVKENKVRAVQAGIMRPLVEMMSDLGSNMVDKSAFVLSLLVPLTEARAALVEEGGIPV 494
LCS KENK+RAVQ+GIM+PLVE+M+D GSNMVDKSAFV+SLL+ + E++ A+VEEGG+PV
Sbjct 214 LCSAKENKIRAVQSGIMKPLVELMADFGSNMVDKSAFVMSLLMSVPESKPAIVEEGGVPV 273
Query 493 LVEIVEVGTQRQKEIAVAILLQLCEDCVSYRTMVAREGAIPPLVALSQSGTSRAKQKAEA 314
LVEIVEVGTQRQKE+AV+ILLQLCE+ V YRTMVAREGAIPPLVALSQ+GTSRAKQKAEA
Sbjct 274 LVEIVEVGTQRQKEMAVSILLQLCEESVVYRTMVAREGAIPPLVALSQAGTSRAKQKAEA 333
Query 313 LIEVLRQPRS-TNGAANN 263
LIE+LRQPRS +NG A +
Sbjct 334 LIELLRQPRSISNGGARS 351
>ref|NP_566136.1| armadillo/beta-catenin-like repeat-containing protein [Arabidopsis
thaliana]
gb|AAF24610.1|AC010870_3 unknown protein [Arabidopsis thaliana]
gb|AEE73661.1| armadillo/beta-catenin-like repeat-containing protein [Arabidopsis
thaliana]
Length=355
Score = 213 bits (542), Expect = 3e-64
Identities = 109/138 (79%), Positives = 128/138 (93%), Gaps = 1/138 (1%)
Frame = -1
Query 673 LCSVKENKVRAVQAGIMRPLVEMMSDLGSNMVDKSAFVLSLLVPLTEARAALVEEGGIPV 494
LCS KENK+RAVQ+GIM+PLVE+M+D GSNMVDKSAFV+SLL+ + E++ A+VEEGG+PV
Sbjct 214 LCSAKENKIRAVQSGIMKPLVELMADFGSNMVDKSAFVMSLLMSVPESKPAIVEEGGVPV 273
Query 493 LVEIVEVGTQRQKEIAVAILLQLCEDCVSYRTMVAREGAIPPLVALSQSGTSRAKQKAEA 314
LVEIVEVGTQRQKE+AV+ILLQLCE+ V YRTMVAREGAIPPLVALSQ+GTSRAKQKAEA
Sbjct 274 LVEIVEVGTQRQKEMAVSILLQLCEESVVYRTMVAREGAIPPLVALSQAGTSRAKQKAEA 333
Query 313 LIEVLRQPRS-TNGAANN 263
LIE+LRQPRS +NG A +
Sbjct 334 LIELLRQPRSISNGGARS 351
>ref|XP_002510099.1| E3 ubiquitin ligase PUB14, putative [Ricinus communis]
gb|EEF52286.1| E3 ubiquitin ligase PUB14, putative [Ricinus communis]
Length=352
Score = 212 bits (539), Expect = 9e-64
Identities = 110/135 (81%), Positives = 123/135 (91%), Gaps = 0/135 (0%)
Frame = -1
Query 673 LCSVKENKVRAVQAGIMRPLVEMMSDLGSNMVDKSAFVLSLLVPLTEARAALVEEGGIPV 494
LCSVKENK+RAVQAGIM+PLVE+M+D SNMVDKSAFVLSLLV +TEAR ALVEEGGIPV
Sbjct 211 LCSVKENKMRAVQAGIMKPLVELMADFESNMVDKSAFVLSLLVSVTEARTALVEEGGIPV 270
Query 493 LVEIVEVGTQRQKEIAVAILLQLCEDCVSYRTMVAREGAIPPLVALSQSGTSRAKQKAEA 314
LVEI+EVG+QRQKEIAVAILLQ+CED + R MV REGAIPPL+ALSQSGT+RAKQKAE
Sbjct 271 LVEIIEVGSQRQKEIAVAILLQICEDNLMRRAMVVREGAIPPLIALSQSGTNRAKQKAET 330
Query 313 LIEVLRQPRSTNGAA 269
LI++LRQPRS N AA
Sbjct 331 LIDLLRQPRSGNAAA 345
>ref|XP_003557013.1| PREDICTED: U-box domain-containing protein 4-like [Glycine max]
Length=319
Score = 208 bits (530), Expect = 8e-63
Identities = 108/134 (81%), Positives = 122/134 (91%), Gaps = 0/134 (0%)
Frame = -1
Query 673 LCSVKENKVRAVQAGIMRPLVEMMSDLGSNMVDKSAFVLSLLVPLTEARAALVEEGGIPV 494
LC VKENK+RAV+AGIM+ LVE+M+D SNMVDKSA+V+S+LV + EARAALVEEGG+PV
Sbjct 178 LCMVKENKIRAVKAGIMKVLVELMADFESNMVDKSAYVVSVLVAVAEARAALVEEGGVPV 237
Query 493 LVEIVEVGTQRQKEIAVAILLQLCEDCVSYRTMVAREGAIPPLVALSQSGTSRAKQKAEA 314
LVEIVEVGTQRQKEI V ILLQ+CED V+YRTMVAREGAIPPLVALSQSGT+RAKQKAE
Sbjct 238 LVEIVEVGTQRQKEIVVVILLQVCEDSVAYRTMVAREGAIPPLVALSQSGTNRAKQKAEK 297
Query 313 LIEVLRQPRSTNGA 272
LIE+LRQPRS GA
Sbjct 298 LIELLRQPRSGYGA 311
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 303068037
Number of extensions: 5801330
Number of successful extensions: 16134
Number of sequences better than 1e-10: 4
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 16111
Number of HSP's successfully gapped: 4
Length of query: 673
Length of database: 6150218869
Length adjustment: 135
Effective length of query: 538
Effective length of database: 3731142529
Effective search space: 332071685081
Effective search space used: 332071685081
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 174 (71.6 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCX78XA01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_494
Length=1132
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002331830.1| predicted protein [Populus trichocarpa] >... 320 3e-104
dbj|BAC11888.1| acidic endochitinase [Arabidopsis halleri sub... 311 1e-100
dbj|BAC11884.1| acidic chitinase [Arabidopsis halleri subsp. ... 311 1e-100
dbj|BAC11891.1| acidic endochitinase [Arabidopsis halleri sub... 310 2e-100
dbj|BAC11890.1| acidic endochitinase [Arabidopsis halleri sub... 310 2e-100
ALIGNMENTS
>ref|XP_002331830.1| predicted protein [Populus trichocarpa]
gb|EEF12199.1| predicted protein [Populus trichocarpa]
Length=296
Score = 320 bits (819), Expect = 3e-104
Identities = 160/280 (57%), Positives = 201/280 (72%), Gaps = 11/280 (4%)
Frame = +2
Query 26 GSISIYWGQNGGEVNLKKTCATSRFAFVNIAFLSQFGSGQIPILNLAGHCDPSSNECTYI 205
G ISIYWGQNG E L +TCAT R+A+VNIAFL++FG+GQ P +NLAGHC+P++ CT +
Sbjct 27 GGISIYWGQNGNEGTLAQTCATGRYAYVNIAFLNKFGNGQTPEMNLAGHCNPANGGCTIV 86
Query 206 SKEIKYCQQQGIKVMLSIGGADGNYNLTTKKDANHVATYLFNNYLAGNSTARPLGDAVLD 385
S IK CQQQGIKV+LS+GG GNY+L +K DA +VA YL+NN+L G S++RPLGDAVLD
Sbjct 87 SGGIKSCQQQGIKVLLSLGGGIGNYSLASKGDAKNVADYLWNNFLGGQSSSRPLGDAVLD 146
Query 386 GIDLAISQGSPEYYHTLVKYLRskdkkvlkskdkkiliSAAPFCPFPDKHLGKALASTRF 565
GID I QGS Y+ L ++L +K+ ++AAP CPFPD +LG AL + F
Sbjct 147 GIDFDIEQGSTLYWEDLARFL--------SKYGRKVYLAAAPQCPFPDSNLGTALNTGLF 198
Query 566 DYVWVQFNQRHQCEYNKLNSKKLIESWKAWTTSKKVKSAEIFLGLMAEES-IGSGYIPAS 742
DYVWVQF C+Y+ N+ L+ SW WTTS + + +IFLGL A S GSGYIP
Sbjct 199 DYVWVQFYNNRPCQYSSGNTTNLLNSWNRWTTS--IDAGKIFLGLPAAPSAAGSGYIPPD 256
Query 743 VLTSKIIPMIRNSTKYGGVMLWSKYWDEVGNYSPKIIDSV 862
VLTS+I+P+I+ S KYGGVMLWSKYWD+ YSP II SV
Sbjct 257 VLTSQILPVIKKSPKYGGVMLWSKYWDDQNGYSPSIISSV 296
>dbj|BAC11888.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
Length=302
Score = 311 bits (796), Expect = 1e-100
Identities = 151/280 (54%), Positives = 201/280 (72%), Gaps = 8/280 (3%)
Frame = +2
Query 26 GSISIYWGQNGGEVNLKKTCATSRFAFVNIAFLSQFGSGQIPILNLAGHCDPSSNECTYI 205
G I+IYWGQNG E NL TCAT R+A+VN+AFL +FG+GQ P LNLAGHC+P++N CT+
Sbjct 30 GGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPELNLAGHCNPAANTCTHF 89
Query 206 SKEIKYCQQQGIKVMLSIGGADGNYNLTTKKDANHVATYLFNNYLAGNSTARPLGDAVLD 385
++KYCQ +GIKVMLS+GG GNY++ +K+DA VA YL+NN+L G S++RPLGDAVLD
Sbjct 90 GSQVKYCQSRGIKVMLSLGGGIGNYSIGSKEDAKVVADYLWNNFLGGKSSSRPLGDAVLD 149
Query 386 GIDLAISQGSPEYYHTLVKYLRskdkkvlkskdkkiliSAAPFCPFPDKHLGKALASTRF 565
GID I GSP+++ L + L L + +K+ ++ AP CPFPD+ +G AL + RF
Sbjct 150 GIDFNIELGSPQHWDDLARSL-----SKLSHRGRKVYLTGAPQCPFPDRLMGNALNTRRF 204
Query 566 DYVWVQFNQRHQCEYNKLNSKKLIESWKAWTTSKKVKSAEIFLGL-MAEESIGSGYIPAS 742
DYVW+QF C Y N++ L +SW WTTS + + +IFLGL A E+ GSGYIP
Sbjct 205 DYVWIQFYNNPPCSYTSGNTQNLFDSWNKWTTS--ITAQKIFLGLPAAPEASGSGYIPPD 262
Query 743 VLTSKIIPMIRNSTKYGGVMLWSKYWDEVGNYSPKIIDSV 862
VLTS+I+P ++ S KYGGVMLWSK+WD+ YS I+ +V
Sbjct 263 VLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSILATV 302
>dbj|BAC11884.1| acidic chitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11886.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11887.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11892.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11894.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11895.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11896.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11897.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
Length=302
Score = 311 bits (796), Expect = 1e-100
Identities = 151/280 (54%), Positives = 201/280 (72%), Gaps = 8/280 (3%)
Frame = +2
Query 26 GSISIYWGQNGGEVNLKKTCATSRFAFVNIAFLSQFGSGQIPILNLAGHCDPSSNECTYI 205
G I+IYWGQNG E NL TCAT R+A+VN+AFL +FG+GQ P LNLAGHC+P++N CT+
Sbjct 30 GGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPELNLAGHCNPAANTCTHF 89
Query 206 SKEIKYCQQQGIKVMLSIGGADGNYNLTTKKDANHVATYLFNNYLAGNSTARPLGDAVLD 385
++KYCQ +GIKVMLS+GG GNY++ +K+DA VA YL+NN+L G S++RPLGDAVLD
Sbjct 90 GSQVKYCQSRGIKVMLSLGGGIGNYSIGSKEDAKVVADYLWNNFLGGKSSSRPLGDAVLD 149
Query 386 GIDLAISQGSPEYYHTLVKYLRskdkkvlkskdkkiliSAAPFCPFPDKHLGKALASTRF 565
GID I GSP+++ L + L L + +K+ ++ AP CPFPD+ +G AL + RF
Sbjct 150 GIDFNIELGSPQHWDDLARSL-----SKLSHRGRKVYLTGAPQCPFPDRLMGNALNTRRF 204
Query 566 DYVWVQFNQRHQCEYNKLNSKKLIESWKAWTTSKKVKSAEIFLGL-MAEESIGSGYIPAS 742
DYVW+QF C Y N++ L +SW WTTS + + +IFLGL A E+ GSGYIP
Sbjct 205 DYVWIQFYNNPPCSYTSGNTQNLFDSWNKWTTS--ITAQKIFLGLPAAPEASGSGYIPPD 262
Query 743 VLTSKIIPMIRNSTKYGGVMLWSKYWDEVGNYSPKIIDSV 862
VLTS+I+P ++ S KYGGVMLWSK+WD+ YS I+ +V
Sbjct 263 VLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSILATV 302
>dbj|BAC11891.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
Length=302
Score = 310 bits (795), Expect = 2e-100
Identities = 151/280 (54%), Positives = 200/280 (71%), Gaps = 8/280 (3%)
Frame = +2
Query 26 GSISIYWGQNGGEVNLKKTCATSRFAFVNIAFLSQFGSGQIPILNLAGHCDPSSNECTYI 205
G I+IYWGQNG E NL TCAT R+A+VN+AFL +FG+GQ P LNLAGHC+P++N CT+
Sbjct 30 GGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPELNLAGHCNPAANTCTHF 89
Query 206 SKEIKYCQQQGIKVMLSIGGADGNYNLTTKKDANHVATYLFNNYLAGNSTARPLGDAVLD 385
++KYCQ +GIKVMLS+GG GNY++ +K+DA VA YL+NN+L G S++RPLGDAVLD
Sbjct 90 GSQVKYCQSRGIKVMLSLGGGIGNYSIGSKEDAKVVADYLWNNFLGGKSSSRPLGDAVLD 149
Query 386 GIDLAISQGSPEYYHTLVKYLRskdkkvlkskdkkiliSAAPFCPFPDKHLGKALASTRF 565
GID I GSP+++ L + L L + +K+ ++ AP CPFPD+ +G AL + RF
Sbjct 150 GIDFNIELGSPQHWDDLARSL-----SKLSHRGRKVYLTGAPQCPFPDRLMGNALNTRRF 204
Query 566 DYVWVQFNQRHQCEYNKLNSKKLIESWKAWTTSKKVKSAEIFLGL-MAEESIGSGYIPAS 742
DYVW+QF C Y N++ L +SW WTTS + + IFLGL A E+ GSGYIP
Sbjct 205 DYVWIQFYNNPPCSYTSGNTQNLFDSWNKWTTS--ITAQRIFLGLPAAPEASGSGYIPPD 262
Query 743 VLTSKIIPMIRNSTKYGGVMLWSKYWDEVGNYSPKIIDSV 862
VLTS+I+P ++ S KYGGVMLWSK+WD+ YS I+ +V
Sbjct 263 VLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSILATV 302
>dbj|BAC11890.1| acidic endochitinase [Arabidopsis halleri subsp. gemmifera]
dbj|BAC11893.1| acidic chitinase [Arabidopsis halleri subsp. gemmifera]
Length=302
Score = 310 bits (795), Expect = 2e-100
Identities = 151/280 (54%), Positives = 201/280 (72%), Gaps = 8/280 (3%)
Frame = +2
Query 26 GSISIYWGQNGGEVNLKKTCATSRFAFVNIAFLSQFGSGQIPILNLAGHCDPSSNECTYI 205
G I+IYWGQNG E NL TCAT R+A+VN+AFL +FG+GQ P LNLAGHC+P++N CT+
Sbjct 30 GGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPELNLAGHCNPAANTCTHF 89
Query 206 SKEIKYCQQQGIKVMLSIGGADGNYNLTTKKDANHVATYLFNNYLAGNSTARPLGDAVLD 385
++KYCQ +GIKVMLS+GG GNY++ +K+DA VA YL+NN+L G S++RPLGDAVLD
Sbjct 90 GSQVKYCQSRGIKVMLSLGGGIGNYSIGSKEDAKVVADYLWNNFLGGKSSSRPLGDAVLD 149
Query 386 GIDLAISQGSPEYYHTLVKYLRskdkkvlkskdkkiliSAAPFCPFPDKHLGKALASTRF 565
GID I GSP+++ L + L L + +K+ ++ AP CPFPD+ +G AL + RF
Sbjct 150 GIDFNIELGSPQHWDDLARSL-----SKLSHRGRKVYLTGAPQCPFPDRLMGNALNTRRF 204
Query 566 DYVWVQFNQRHQCEYNKLNSKKLIESWKAWTTSKKVKSAEIFLGL-MAEESIGSGYIPAS 742
DYVW+QF C Y N++ L +SW WTTS + + +IFLGL A E+ GSGYIP
Sbjct 205 DYVWIQFYNNPPCSYTSGNTQNLFDSWNKWTTS--ITAQKIFLGLPAAPEASGSGYIPPD 262
Query 743 VLTSKIIPMIRNSTKYGGVMLWSKYWDEVGNYSPKIIDSV 862
VLTS+I+P ++ S KYGGVMLWSK+WD+ YS I+ +V
Sbjct 263 VLTSQILPTLKKSRKYGGVMLWSKFWDDKDGYSSSILATV 302
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 577945536
Number of extensions: 12844199
Number of successful extensions: 34514
Number of sequences better than 1e-10: 40
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 34329
Number of HSP's successfully gapped: 40
Length of query: 1132
Length of database: 6150218869
Length adjustment: 142
Effective length of query: 990
Effective length of database: 3605708941
Effective search space: 847341601135
Effective search space used: 847341601135
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCXS2ED01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_512
Length=1126
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002265108.2| PREDICTED: uncharacterized protein LOC100... 181 1e-47
emb|CBI25715.3| unnamed protein product [Vitis vinifera] 179 4e-47
ref|XP_003612947.1| Glycine-rich protein [Medicago truncatula... 173 1e-44
ref|XP_003517161.1| PREDICTED: uncharacterized protein LOC100... 172 2e-44
ref|XP_002321500.1| predicted protein [Populus trichocarpa] >... 171 5e-44
ALIGNMENTS
>ref|XP_002265108.2| PREDICTED: uncharacterized protein LOC100252003 [Vitis vinifera]
Length=825
Score = 181 bits (459), Expect = 1e-47
Identities = 96/203 (47%), Positives = 127/203 (63%), Gaps = 5/203 (2%)
Frame = -3
Query 1124 RSSRLFPQPVKVQFAKSWTHVVDDAGNLVMSLQMRGSKKSKGKIEGM-RREVVGLMESGQ 948
RSS FP P ++Q AK WT V+D+AG+ V+SLQMR SKK + + RREV+G+ S +
Sbjct 478 RSSCFFPLPGRIQHAKRWTRVIDEAGSEVISLQMRDSKKGTARDTSVSRREVIGVTTSLE 537
Query 947 KCTLAEFAGSKWSFVNSSWSLQLPNSENDDGHLLGLTGPYTVRLYGGEKLCFESKQCKKH 768
TLAEF G+ WS ++ +W L+ DGHL L G V++Y G KL FE K C++
Sbjct 538 TITLAEFVGTGWSLMDYNWCLKFEKKSGKDGHLFELVGNRMVKIYPGRKLEFEHKHCERQ 597
Query 767 NSEYELDTHLITAIEFSEKDPYGKAVALLDMKSGIVKVKEEWFLLPGFILTFILGSILRK 588
S++ +TA+EFS + PYG+AVALLD+KSG +KV EEW +LPG IL FIL ILRK
Sbjct 598 KSDHGF----LTAVEFSAEVPYGRAVALLDLKSGFLKVNEEWLVLPGIILVFILSDILRK 653
Query 587 EGYKGRVMGIRTLKKKRATKGNY 519
EG + LK+ G Y
Sbjct 654 EGCDSFTVSEGNLKETENLSGCY 676
>emb|CBI25715.3| unnamed protein product [Vitis vinifera]
Length=648
Score = 179 bits (453), Expect = 4e-47
Identities = 92/182 (51%), Positives = 121/182 (66%), Gaps = 5/182 (3%)
Frame = -3
Query 1124 RSSRLFPQPVKVQFAKSWTHVVDDAGNLVMSLQMRGSKKSKGKIEGM-RREVVGLMESGQ 948
RSS FP P ++Q AK WT V+D+AG+ V+SLQMR SKK + + RREV+G+ S +
Sbjct 452 RSSCFFPLPGRIQHAKRWTRVIDEAGSEVISLQMRDSKKGTARDTSVSRREVIGVTTSLE 511
Query 947 KCTLAEFAGSKWSFVNSSWSLQLPNSENDDGHLLGLTGPYTVRLYGGEKLCFESKQCKKH 768
TLAEF G+ WS ++ +W L+ DGHL L G V++Y G KL FE K C++
Sbjct 512 TITLAEFVGTGWSLMDYNWCLKFEKKSGKDGHLFELVGNRMVKIYPGRKLEFEHKHCERQ 571
Query 767 NSEYELDTHLITAIEFSEKDPYGKAVALLDMKSGIVKVKEEWFLLPGFILTFILGSILRK 588
S++ +TA+EFS + PYG+AVALLD+KSG +KV EEW +LPG IL FIL ILRK
Sbjct 572 KSDHGF----LTAVEFSAEVPYGRAVALLDLKSGFLKVNEEWLVLPGIILVFILSDILRK 627
Query 587 EG 582
EG
Sbjct 628 EG 629
>ref|XP_003612947.1| Glycine-rich protein [Medicago truncatula]
gb|ABY48136.1| glycine-rich protein [Medicago truncatula]
gb|AES95905.1| Glycine-rich protein [Medicago truncatula]
Length=897
Score = 173 bits (439), Expect = 1e-44
Identities = 86/193 (45%), Positives = 127/193 (66%), Gaps = 5/193 (3%)
Frame = -3
Query 1124 RSSRLFPQPVKVQFAKSWTHVVDDAGNLVMSLQMRGSKKSKGKIEGMRREVVGLMESGQK 945
+++ F PVK Q AKSWTH D+ G ++SLQMR K +K +E + +EV GLMESG+
Sbjct 475 KNTCFFNLPVKPQQAKSWTHATDENGTRIISLQMRDLKNAKN-VENLGKEVAGLMESGET 533
Query 944 CTLAEFAGSKWSFVNSSWSLQLPNSENDDGHLLGLTGPYTVRLYGGEKLCFESKQCKKHN 765
TLAE+ + WSF+++ W L P+ +DGH+ LTG T++++ G K +E + K
Sbjct 534 RTLAEYMENGWSFMDNLWLLHRPSKSKNDGHIFELTGTKTIKIFSGRKGEYELRYHLKQG 593
Query 764 SEYELDTHLITAIEFSEKDPYGKAVALLDMKSGIVKVKEEWFLLPGFILTFILGSILRKE 585
+E + +TA+EFS +DPYGKAVALLD+KS +V KE+W +LPG IL F+ I++KE
Sbjct 594 NEMDF----LTAVEFSIEDPYGKAVALLDLKSNLVSAKEKWMVLPGIILAFLASDIMKKE 649
Query 584 GYKGRVMGIRTLK 546
GY+G + + L+
Sbjct 650 GYEGIIAKSKDLE 662
>ref|XP_003517161.1| PREDICTED: uncharacterized protein LOC100810300 [Glycine max]
Length=852
Score = 172 bits (437), Expect = 2e-44
Identities = 94/207 (45%), Positives = 132/207 (64%), Gaps = 6/207 (3%)
Frame = -3
Query 1124 RSSRLFPQPVKVQFAKSWTHVVDDAGNLVMSLQMRGSKKSKGKIEGMRREVVGLMESGQK 945
+++ LF PV+ Q AKSWTHV D+ G ++SLQMR K +K I +EVVGLM+SG+
Sbjct 475 KNTCLFNLPVRPQHAKSWTHVTDENGTRIISLQMRDLKNAKN-IGNPGKEVVGLMKSGET 533
Query 944 CTLAEFAGSKWSFVNSSWSLQLPNSENDDGHLLGLTGPYT-VRLYGGEKLCFESKQCKKH 768
TLAEF + WS + + W LPN +DGHL LTG VR++ G KL +E + H
Sbjct 534 RTLAEFMENGWSILENLWLFHLPNKSTNDGHLFELTGANKRVRIFPGRKLDYELR----H 589
Query 767 NSEYELDTHLITAIEFSEKDPYGKAVALLDMKSGIVKVKEEWFLLPGFILTFILGSILRK 588
N + + + +TA+EFS ++PYGKAVALLD++S V KE+W +LPG ILTFI +I++K
Sbjct 590 NGKRGNEMNFLTAVEFSIEEPYGKAVALLDLRSRHVTAKEKWMVLPGIILTFIASNIMKK 649
Query 587 EGYKGRVMGIRTLKKKRATKGNYVQIL 507
EGY+G + + LK + N +L
Sbjct 650 EGYEGIIAKSKDLKVNGPNEENEKTVL 676
>ref|XP_002321500.1| predicted protein [Populus trichocarpa]
gb|EEF05627.1| predicted protein [Populus trichocarpa]
Length=789
Score = 171 bits (432), Expect = 5e-44
Identities = 89/186 (48%), Positives = 120/186 (65%), Gaps = 6/186 (3%)
Frame = -3
Query 1124 RSSRLFPQPVKVQFAKSWTHVVDDAGNLVMSLQMRGSKKSKGKIEG-MRREVVGLMESGQ 948
+SS FP P + Q WT VV+ ++SLQMR S K+K K ++++V G+M++G+
Sbjct 475 KSSCFFPLPGRAQHPNIWTSVVEKTDAEIISLQMRNSTKAKEKERSILKQQVTGVMKTGE 534
Query 947 KCTLAEFAGSKWSFVNSSWSLQLPNSENDDGHLLGLTG-PYTVRLYGGEKLCFESKQCKK 771
C LAEF G++W ++S W L+ N+DGHL L G V+L+ G+KL FE K C+K
Sbjct 535 TCILAEFVGTRWCLMDSQWYLEPKKKSNEDGHLFELIGCRMVVKLFQGKKLDFEPKHCEK 594
Query 770 HNSEYELDTHLITAIEFSEKDPYGKAVALLDMKSGIVKVKEEWFLLPGFILTFILGSILR 591
S+ + +TA+EFS + PYGKAVALLD+KSG VKVKE W LLP I FIL IL+
Sbjct 595 KRSKQDF----MTAVEFSAEYPYGKAVALLDLKSGFVKVKESWLLLPAIISAFILSDILK 650
Query 590 KEGYKG 573
KEGY G
Sbjct 651 KEGYNG 656
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 502552314
Number of extensions: 10107960
Number of successful extensions: 24345
Number of sequences better than 1e-10: 2
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 24331
Number of HSP's successfully gapped: 2
Length of query: 1126
Length of database: 6150218869
Length adjustment: 142
Effective length of query: 984
Effective length of database: 3605708941
Effective search space: 840130183253
Effective search space used: 840130183253
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 178 (73.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD94XFR01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_5440
Length=639
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 351924396
Number of extensions: 8826533
Number of successful extensions: 44806
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 40719
Number of HSP's successfully gapped: 0
Length of query: 639
Length of database: 6150218869
Length adjustment: 135
Effective length of query: 504
Effective length of database: 3731142529
Effective search space: 291029117262
Effective search space used: 291029117262
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 174 (71.6 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPD9AMMT012
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_5467
Length=638
Score E
Sequences producing significant alignments: (Bits) Value
ref|NP_001234666.1| protein RSI-1 precursor [Solanum lycopers... 89.4 9e-20
ref|XP_003616284.1| GASA5-like protein [Medicago truncatula] ... 87.4 5e-19
ref|NP_566186.1| GAST1 protein homolog 5 [Arabidopsis thalian... 82.8 3e-17
ref|XP_002301708.1| predicted protein [Populus trichocarpa] >... 82.4 3e-17
ref|XP_002531185.1| RSI-1 protein precursor, putative [Ricinu... 81.6 6e-17
ALIGNMENTS
>ref|NP_001234666.1| protein RSI-1 precursor [Solanum lycopersicum]
sp|P47926.1|RSI1_SOLLC RecName: Full=Protein RSI-1; AltName: Full=TR132; Flags: Precursor
gb|AAA20129.1| RSI-1 protein [Solanum lycopersicum]
gb|AAA20130.1| RSI-1 protein [Solanum lycopersicum]
Length=96
Score = 89.4 bits (220), Expect = 9e-20
Identities = 52/73 (71%), Positives = 60/73 (82%), Gaps = 0/73 (0%)
Frame = +3
Query 72 SMVAEGKHKLKKKDCKPRCAFRCSATSHkkpcllyckkcctkclcvppGTSGNKQVCPCY 251
S V EG +KL+ DCKPRC +RCSATSHKKPC+ +C+KCC CLCVP G GNKQ CPCY
Sbjct 24 SNVVEGYNKLRPTDCKPRCTYRCSATSHKKPCMFFCQKCCATCLCVPKGVYGNKQSCPCY 83
Query 252 NNWKTQQGRPKCP 290
NNWKTQ+G+PKCP
Sbjct 84 NNWKTQEGKPKCP 96
>ref|XP_003616284.1| GASA5-like protein [Medicago truncatula]
gb|AES99242.1| GASA5-like protein [Medicago truncatula]
Length=96
Score = 87.4 bits (215), Expect = 5e-19
Identities = 51/70 (73%), Positives = 61/70 (87%), Gaps = 0/70 (0%)
Frame = +3
Query 81 AEGKHKLKKKDCKPRCAFRCSATSHkkpcllyckkcctkclcvppGTSGNKQVCPCYNNW 260
A G+ KL+ +DCKPRC +RCSATSHKKPC+ +C+KCC CLCVPPG GNKQVCPCYN+W
Sbjct 27 AYGRAKLRPQDCKPRCTYRCSATSHKKPCMFFCQKCCATCLCVPPGVYGNKQVCPCYNSW 86
Query 261 KTQQGRPKCP 290
KTQ+G+PKCP
Sbjct 87 KTQEGKPKCP 96
>ref|NP_566186.1| GAST1 protein homolog 5 [Arabidopsis thaliana]
sp|Q84J95.1|GASA5_ARATH RecName: Full=Gibberellin-regulated protein 5; AltName: Full=GAST1
protein homolog 5; Flags: Precursor
gb|AAO22614.1| unknown protein [Arabidopsis thaliana]
gb|AAO42349.1| unknown protein [Arabidopsis thaliana]
gb|AEE73874.1| GAST1 protein homolog 5 [Arabidopsis thaliana]
Length=97
Score = 82.8 bits (203), Expect = 3e-17
Identities = 51/70 (73%), Positives = 58/70 (83%), Gaps = 0/70 (0%)
Frame = +3
Query 81 AEGKHKLKKKDCKPRCAFRCSATSHkkpcllyckkcctkclcvppGTSGNKQVCPCYNNW 260
A G KLK + C +C+FRCSATSHKKPC+ +C KCC KCLCVPPGT GNKQ CPCYNNW
Sbjct 28 ARGGGKLKPQQCNSKCSFRCSATSHKKPCMFFCLKCCKKCLCVPPGTFGNKQTCPCYNNW 87
Query 261 KTQQGRPKCP 290
KT++GRPKCP
Sbjct 88 KTKEGRPKCP 97
>ref|XP_002301708.1| predicted protein [Populus trichocarpa]
gb|EEE80981.1| predicted protein [Populus trichocarpa]
Length=95
Score = 82.4 bits (202), Expect = 3e-17
Identities = 48/65 (74%), Positives = 56/65 (86%), Gaps = 0/65 (0%)
Frame = +3
Query 96 KLKKKDCKPRCAFRCSATSHkkpcllyckkcctkclcvppGTSGNKQVCPCYNNWKTQQG 275
KL+ +CKPRC +RCSATSHKKPC+ +C KCC CLCVPPGT GNK+ CPCYNNWKT++G
Sbjct 31 KLRPSECKPRCNYRCSATSHKKPCMFFCLKCCATCLCVPPGTYGNKETCPCYNNWKTKEG 90
Query 276 RPKCP 290
RPKCP
Sbjct 91 RPKCP 95
>ref|XP_002531185.1| RSI-1 protein precursor, putative [Ricinus communis]
gb|EEF31200.1| RSI-1 protein precursor, putative [Ricinus communis]
Length=95
Score = 81.6 bits (200), Expect = 6e-17
Identities = 48/65 (74%), Positives = 57/65 (88%), Gaps = 0/65 (0%)
Frame = +3
Query 96 KLKKKDCKPRCAFRCSATSHkkpcllyckkcctkclcvppGTSGNKQVCPCYNNWKTQQG 275
+ + DCKPRC +RCSATSHKKPC+ +C KCC+KCLCVPPG GNKQVCPCYNNWKT++G
Sbjct 31 RFRPSDCKPRCTYRCSATSHKKPCMFFCLKCCSKCLCVPPGVYGNKQVCPCYNNWKTKEG 90
Query 276 RPKCP 290
+PKCP
Sbjct 91 KPKCP 95
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 5596461065
Number of extensions: 104333648
Number of successful extensions: 310821
Number of sequences better than 1e-10: 70
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 308918
Number of HSP's successfully gapped: 70
Length of query: 638
Length of database: 6150218869
Length adjustment: 135
Effective length of query: 503
Effective length of database: 3731142529
Effective search space: 287297974733
Effective search space used: 287297974733
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 174 (71.6 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDA2PVH01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_5684
Length=629
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 350756732
Number of extensions: 7906766
Number of successful extensions: 20777
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 20741
Number of HSP's successfully gapped: 0
Length of query: 629
Length of database: 6150218869
Length adjustment: 134
Effective length of query: 495
Effective length of database: 3749061613
Effective search space: 281179620975
Effective search space used: 281179620975
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 174 (71.6 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCXUT1S01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_571
Length=1103
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003554616.1| PREDICTED: uncharacterized protein At5g39... 85.9 7e-16
ref|XP_002518496.1| conserved hypothetical protein [Ricinus c... 79.0 5e-15
ref|XP_003543859.1| PREDICTED: uncharacterized protein At5g39... 82.0 2e-14
emb|CAN76789.1| hypothetical protein VITISV_038837 [Vitis vin... 77.0 2e-14
gb|AAN60241.1| unknown [Arabidopsis thaliana] 80.5 6e-14
ALIGNMENTS
>ref|XP_003554616.1| PREDICTED: uncharacterized protein At5g39570-like [Glycine max]
Length=363
Score = 85.9 bits (211), Expect = 7e-16
Identities = 65/131 (50%), Positives = 72/131 (55%), Gaps = 30/131 (23%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYG-RKAEPEYGSGYG-RKAE 929
GRK E EYGSGY RK E EYGS YG RK E EYGSGYG RK E
Sbjct 221 GRKEESEYGSGYDG----------------RKEESEYGSGYGGRKQESEYGSGYGGRKQE 264
Query 928 PEYESGYGRRSENEEQ--YGRKP-ESE------EYGYVRKPERNEYEEEKPSYERSSYQ- 779
EY SGYG RSE EE+ YGR ES+ EYGY +P R E EEE+ Y + SYQ
Sbjct 265 SEYGSGYGGRSEYEEKPSYGRSNYESQGGGGGVEYGYEGRPPRQE-EEEEEGYRKPSYQR 323
Query 778 -SEHERPSYGR 749
+ + YGR
Sbjct 324 KDDDDEEGYGR 334
Score = 81.3 bits (199), Expect = 3e-14
Identities = 59/115 (51%), Positives = 66/115 (57%), Gaps = 23/115 (20%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYG-RKAEPEYGSGY-GRKAE 929
GRK E EYGSGYG RK E EYGS YG RK E EYGSGY GRK E
Sbjct 195 GRKQESEYGSGYGG----------------RKEESEYGSGYGGRKEESEYGSGYDGRKEE 238
Query 928 PEYESGYGRRSENEEQ---YGRKPESEEYGYVRKPERNEYEEEKPSYERSSYQSE 773
EY SGYG R + E YG + + EYG R+EY EEKPSY RS+Y+S+
Sbjct 239 SEYGSGYGGRKQESEYGSGYGGRKQESEYG-SGYGGRSEY-EEKPSYGRSNYESQ 291
Score = 77.8 bits (190), Expect = 5e-13
Identities = 61/128 (48%), Positives = 70/128 (55%), Gaps = 16/128 (13%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYGRKAEPEYGSGY-GRKAEP 926
GRK E EYGSGYGRK E G RK E EYGS YGRK E EYGSGY GRK +
Sbjct 107 GRKQESEYGSGYGRKEEGEYGSGYGG----RKEESEYGSGYGRKEEGEYGSGYGGRKEDS 162
Query 925 EYESGYGRRSENE--EQYGRKPESEEY--GYVRKPERNEY------EEEKPSYERSSYQS 776
EY SGYGR+ E+E YG + E EY GY + + +EY +E+ Y S Y
Sbjct 163 EYGSGYGRKQEDEFGSGYGGRKEESEYGSGYGGRKQESEYGSGYGGRKEESEY-GSGYGG 221
Query 775 EHERPSYG 752
E YG
Sbjct 222 RKEESEYG 229
Score = 73.2 bits (178), Expect = 2e-11
Identities = 49/102 (48%), Positives = 53/102 (52%), Gaps = 14/102 (14%)
Frame = -2
Query 1012 RKAEPEYGSEYGRKAEPEYGSGYG-RKAEPEYESGYGRRSENE--EQYGRKPESEEY--G 848
RK E EYGS YGRK E EYGSGYG RK E EY SGYGR+ E E YG + E EY G
Sbjct 108 RKQESEYGSGYGRKEEGEYGSGYGGRKEESEYGSGYGRKEEGEYGSGYGGRKEDSEYGSG 167
Query 847 YVRKPE---------RNEYEEEKPSYERSSYQSEHERPSYGR 749
Y RK E R E E Y +SE+ GR
Sbjct 168 YGRKQEDEFGSGYGGRKEESEYGSGYGGRKQESEYGSGYGGR 209
>ref|XP_002518496.1| conserved hypothetical protein [Ricinus communis]
gb|EEF43883.1| conserved hypothetical protein [Ricinus communis]
Length=110
Score = 79.0 bits (193), Expect = 5e-15
Identities = 44/107 (41%), Positives = 68/107 (64%), Gaps = 7/107 (7%)
Frame = +3
Query 3 LFNKSHAYSKIENEDIEETKHRLAQFLIYKSMKKADIGRRKRPSWLRVKMLKLKTKIGHR 182
+ KS+ YSK++ ED EE HR AQFLIYK++K+AD R++PS+ R++M +LK KIG +
Sbjct 6 MMKKSYGYSKLDKEDPEEIIHRRAQFLIYKALKQAD--SRRKPSFSRIRMCRLKVKIGRK 63
Query 183 LKILKKGFSSTAFSAKVDLCKQMSFVLRSCKHLLVAKQVPIVRTLPT 323
LK L+K + +A++ + KQ ++ K L + + TLPT
Sbjct 64 LKRLRKSMLLSISAARIRIYKQ---IVNQLKRLFSSGDA--IATLPT 105
>ref|XP_003543859.1| PREDICTED: uncharacterized protein At5g39570-like [Glycine max]
Length=374
Score = 82.0 bits (201), Expect = 2e-14
Identities = 62/132 (47%), Positives = 69/132 (52%), Gaps = 31/132 (23%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYG-RKAEPEYGSGYGRKAEP 926
GRK EPEYGSGYGR RK E EYGS YG RK EPEYGSGYGRK E
Sbjct 140 GRKEEPEYGSGYGR----------------RKEEGEYGSGYGGRKEEPEYGSGYGRKEEG 183
Query 925 EYESGY-----GRRSENE--EQYGRKPESEEY--GYVRKPERNEY-----EEEKPSYERS 788
EY SGY GR+ E+E YG + E EY GY + E +EY ++ S S
Sbjct 184 EYGSGYGSGYGGRKEESEYGSGYGGRKEEPEYGSGYGGRKEESEYGSGYGGRKQESEYGS 243
Query 787 SYQSEHERPSYG 752
Y E P YG
Sbjct 244 GYGGRKEEPEYG 255
Score = 78.2 bits (191), Expect = 4e-13
Identities = 60/121 (50%), Positives = 70/121 (58%), Gaps = 11/121 (9%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYG-RKAEPEYGSGYGRKAEP 926
GRK EPEYGSGYG + E E YG RK E EYGS YG RK E EYGSGYG ++E
Sbjct 247 GRKEEPEYGSGYGGRKEESEYGSGYGG---RKQESEYGSGYGGRKEESEYGSGYGGRSEY 303
Query 925 EYESGYGRRSENEEQYGRKPESEEYGYVRKPERNEYEE--EKPSYERSSYQSEHERPSYG 752
E + YGR + + G + EYGY +P R E EE KPSYER + + E YG
Sbjct 304 EEKPSYGRSNYESQGGGGR---VEYGYEGRPPRQEEEEGFRKPSYERKADDDDDE--GYG 358
Query 751 R 749
R
Sbjct 359 R 359
Score = 77.4 bits (189), Expect = 7e-13
Identities = 58/127 (46%), Positives = 61/127 (48%), Gaps = 21/127 (17%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYG-RKAEPEYGSGY-GRKAE 929
GRK E EYGS E GRK EPEYGS YG RK E EYGSGY GRK E
Sbjct 114 GRKQESEYGS---GYGGRKEEGEYGSGYGGRKEEPEYGSGYGRRKEEGEYGSGYGGRKEE 170
Query 928 PEYESGYGRRSENE------EQYGRKPESEEY--GYVRKPERNEYEEEKPSYERSSYQSE 773
PEY SGYGR+ E E YG + E EY GY + E EY S Y
Sbjct 171 PEYGSGYGRKEEGEYGSGYGSGYGGRKEESEYGSGYGGRKEEPEY--------GSGYGGR 222
Query 772 HERPSYG 752
E YG
Sbjct 223 KEESEYG 229
>emb|CAN76789.1| hypothetical protein VITISV_038837 [Vitis vinifera]
Length=108
Score = 77.0 bits (188), Expect = 2e-14
Identities = 42/94 (45%), Positives = 62/94 (66%), Gaps = 2/94 (2%)
Frame = +3
Query 3 LFNKSHAYSKIENEDIEETKHRLAQFLIYKSMKKADIGRRKRPSWLRVKMLKLKTKIGHR 182
L + + YSK+E ED EE HR AQFLIYK M+KAD R++ S+LR+++ +L+ KIG R
Sbjct 3 LVRRPYGYSKMEKEDPEEAIHRRAQFLIYKVMEKAD--SRRKASFLRLRICRLRVKIGRR 60
Query 183 LKILKKGFSSTAFSAKVDLCKQMSFVLRSCKHLL 284
LK +K + +AKV + +Q+ L++ K LL
Sbjct 61 LKRFRKSMMLSVTAAKVGVYRQVLSQLKAWKRLL 94
>gb|AAN60241.1| unknown [Arabidopsis thaliana]
Length=381
Score = 80.5 bits (197), Expect = 6e-14
Identities = 53/112 (47%), Positives = 61/112 (54%), Gaps = 23/112 (21%)
Frame = -2
Query 1012 RKAEPEYGS--------EYGRKAEPEYGSGYGRKAEPEYESGYGRRSENEEQYGRKPES- 860
RK E EYGS EYGR+ E YGSGYG + E E E G G E +YGR+PES
Sbjct 118 RKPESEYGSGYGGQTEVEYGRRPEQSYGSGYGGRTETESEYGSGGGGRTEVEYGRRPESG 177
Query 859 --------EEYGYVRKPE--RNEYEEE---KPSYERSSYQSE-HERPSYGRS 746
E Y RKP R+E +EE KPSY RS Q E + +PSYGRS
Sbjct 178 LGSGYGGRSESEYERKPSYGRSEEQEEGYRKPSYGRSEEQEEGYRKPSYGRS 229
Score = 76.6 bits (187), Expect = 1e-12
Identities = 66/139 (47%), Positives = 76/139 (55%), Gaps = 31/139 (22%)
Frame = -2
Query 1102 GRKAEPEYGSgygrkpeygepepeygsgyGRKAEPEYGSEYGRKAEPE--YGSG------ 947
GRK E EYGSGYG + E R+ E YGS YG + E E YGSG
Sbjct 117 GRKPESEYGSGYGGQTEVEYG---------RRPEQSYGSGYGGRTETESEYGSGGGGRTE 167
Query 946 --YGRKAEPEYESGYGRRSENEEQ----YGRKPESEEYGYVRKPE--RNEYEEE---KPS 800
YGR+ E SGYG RSE+E + YGR E EE GY RKP R+E +EE KPS
Sbjct 168 VEYGRRPESGLGSGYGGRSESEYERKPSYGRSEEQEE-GY-RKPSYGRSEEQEEGYRKPS 225
Query 799 YERSSYQSE-HERPSYGRS 746
Y RS Q E + +PSYGRS
Sbjct 226 YGRSEEQEEGYRKPSYGRS 244
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 491168351
Number of extensions: 9643461
Number of successful extensions: 30446
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 29882
Number of HSP's successfully gapped: 0
Length of query: 1103
Length of database: 6150218869
Length adjustment: 142
Effective length of query: 961
Effective length of database: 3605708941
Effective search space: 811284511725
Effective search space used: 811284511725
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 177 (72.8 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCM12RA016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_59
Length=1524
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003531272.1| PREDICTED: uncharacterized protein LOC100... 475 3e-157
emb|CBI16561.3| unnamed protein product [Vitis vinifera] 471 3e-156
ref|XP_002285750.1| PREDICTED: uncharacterized protein LOC100... 471 7e-156
ref|XP_002304850.1| predicted protein [Populus trichocarpa] >... 454 1e-151
ref|XP_003629923.1| YTH domain family protein [Medicago trunc... 451 4e-148
ALIGNMENTS
>ref|XP_003531272.1| PREDICTED: uncharacterized protein LOC100819200 isoform 1 [Glycine
max]
Length=707
Score = 475 bits (1222), Expect = 3e-157
Identities = 240/368 (65%), Positives = 278/368 (76%), Gaps = 22/368 (6%)
Frame = -3
Query 1522 GQYGNTYRSGVGYGANSYDTRTNGRGWMSVDSKYKPRGRGNGPYNYANESVDGLNELNRG 1343
GQYGNT RSG+GYG + YD+R NGR W++VDSKYK RGR G + Y NE+VDGLNELNRG
Sbjct 340 GQYGNTVRSGMGYGTHGYDSRANGRAWLAVDSKYKTRGRSGGYFGYGNENVDGLNELNRG 399
Query 1342 PRAKSSKNQKGFTPVSLAVKGQSNISLTGTNDSDDKILSLIPDREQYNLPDFPETYANAK 1163
PRAK KNQKGF P LAVKGQ+ + GT++ DK S +PDR+QYN DFPE Y +AK
Sbjct 400 PRAKGGKNQKGFAPTILAVKGQNLPASLGTDEEKDKT-STVPDRDQYNKADFPEEYTDAK 458
Query 1162 FFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAYHEAQQKPGGCPIFLFFSVNTSGQFV 983
FF+IKSYSEDD+HK IKYNVWAST NGNKKLDAAYHEAQQKPGGCP+FLFFSVNTSGQFV
Sbjct 459 FFVIKSYSEDDIHKSIKYNVWASTQNGNKKLDAAYHEAQQKPGGCPVFLFFSVNTSGQFV 518
Query 982 GAAEMTGPVDFNKNVEYWQQDKWVGCFPVKWHIVKDVPNSLLKHITLENNENKPVTNSRD 803
G AEM GPVDFNK+VEYWQQDKW GCFP+KWH+VKDVPN+LL+HITL+NNENKPVTNSRD
Sbjct 519 GLAEMIGPVDFNKSVEYWQQDKWNGCFPLKWHVVKDVPNNLLRHITLDNNENKPVTNSRD 578
Query 802 TQEVKLDQGLQLIKIFKDHTSKQCILDDFDFYEDRQKRIqekkakqqqfqkqaQIWEGKA 623
TQEV L+ GL+LIKIFK++TSK CILDDF FYE RQK I K+ + Q Q+WEGK
Sbjct 579 TQEVMLEPGLKLIKIFKEYTSKTCILDDFGFYEARQKTI---LEKKAKQQFPKQVWEGKP 635
Query 622 TDEKNKESTNGEVKAQQS--------TELASSDLNKEAAQINDVASKTINS--------P 491
DEK + NGEV Q+S LA D + N A+KT ++ P
Sbjct 636 ADEKIE--INGEVNTQKSEVSSEVLKESLAEKDSDDHKVPENGCATKTGDAPKGAKPVVP 693
Query 490 EKVVLANG 467
E ++ANG
Sbjct 694 ESKIVANG 701
>emb|CBI16561.3| unnamed protein product [Vitis vinifera]
Length=675
Score = 471 bits (1213), Expect = 3e-156
Identities = 238/330 (72%), Positives = 272/330 (82%), Gaps = 11/330 (3%)
Frame = -3
Query 1522 GQYGNTYRSGVGYGANSYDTRTNGRGWMSVDSKYKPRGRGNGPYNYANESVDGLNELNRG 1343
GQYGNT RSG+G+G+N+YD+RTNGR W++VD+KYKPRGRG G + Y NE++DGLNELNRG
Sbjct 306 GQYGNTVRSGLGFGSNAYDSRTNGRSWLAVDNKYKPRGRGIGFFGYGNENMDGLNELNRG 365
Query 1342 PRAKSSKNQKGFTPVSLAVKGQSNISLTGTNDSDDKILSLIPDREQYNLPDFPETYANAK 1163
PRAKSSKNQKG PV+ KGQ N+ G+ND + S+ PDR+QYN DFP + AK
Sbjct 366 PRAKSSKNQKGLAPVT--AKGQ-NVQSNGSNDEEKDRTSVFPDRDQYNHADFPVEHTEAK 422
Query 1162 FFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAYHEAQQKPGGCPIFLFFSVNTSGQFV 983
FFIIKSYSEDDVHK IKYNVWASTPNGNKKLDAAY EAQ+K GCP+FLFFSVNTSGQFV
Sbjct 423 FFIIKSYSEDDVHKSIKYNVWASTPNGNKKLDAAYREAQEKSAGCPVFLFFSVNTSGQFV 482
Query 982 GAAEMTGPVDFNKNVEYWQQDKWVGCFPVKWHIVKDVPNSLLKHITLENNENKPVTNSRD 803
G AEM G VDF+KNVEYWQQDKW GCF VKWH+VKDVPNSLLKHITLENNENKPVTNSRD
Sbjct 483 GLAEMVGHVDFHKNVEYWQQDKWNGCFSVKWHVVKDVPNSLLKHITLENNENKPVTNSRD 542
Query 802 TQEVKLDQGLQLIKIFKDHTSKQCILDDFDFYEDRQKRIqekkakqqqfqkqaQIWEGKA 623
TQEVKL+QGLQ++KIFK+H SK CILDDF FYE RQK I ++ K +Q Q Q Q+WEGK
Sbjct 543 TQEVKLEQGLQMLKIFKEHCSKTCILDDFAFYESRQKTI--QEKKAKQQQFQKQVWEGKP 600
Query 622 TDEKNKESTNGEVKAQQSTELASSDLNKEA 533
TDEK NGE+K Q+S E+A SDL KE+
Sbjct 601 TDEK-----NGELKPQKSLEVA-SDLIKES 624
>ref|XP_002285750.1| PREDICTED: uncharacterized protein LOC100253534 [Vitis vinifera]
Length=705
Score = 471 bits (1213), Expect = 7e-156
Identities = 238/330 (72%), Positives = 272/330 (82%), Gaps = 11/330 (3%)
Frame = -3
Query 1522 GQYGNTYRSGVGYGANSYDTRTNGRGWMSVDSKYKPRGRGNGPYNYANESVDGLNELNRG 1343
GQYGNT RSG+G+G+N+YD+RTNGR W++VD+KYKPRGRG G + Y NE++DGLNELNRG
Sbjct 336 GQYGNTVRSGLGFGSNAYDSRTNGRSWLAVDNKYKPRGRGIGFFGYGNENMDGLNELNRG 395
Query 1342 PRAKSSKNQKGFTPVSLAVKGQSNISLTGTNDSDDKILSLIPDREQYNLPDFPETYANAK 1163
PRAKSSKNQKG PV+ KGQ N+ G+ND + S+ PDR+QYN DFP + AK
Sbjct 396 PRAKSSKNQKGLAPVT--AKGQ-NVQSNGSNDEEKDRTSVFPDRDQYNHADFPVEHTEAK 452
Query 1162 FFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAYHEAQQKPGGCPIFLFFSVNTSGQFV 983
FFIIKSYSEDDVHK IKYNVWASTPNGNKKLDAAY EAQ+K GCP+FLFFSVNTSGQFV
Sbjct 453 FFIIKSYSEDDVHKSIKYNVWASTPNGNKKLDAAYREAQEKSAGCPVFLFFSVNTSGQFV 512
Query 982 GAAEMTGPVDFNKNVEYWQQDKWVGCFPVKWHIVKDVPNSLLKHITLENNENKPVTNSRD 803
G AEM G VDF+KNVEYWQQDKW GCF VKWH+VKDVPNSLLKHITLENNENKPVTNSRD
Sbjct 513 GLAEMVGHVDFHKNVEYWQQDKWNGCFSVKWHVVKDVPNSLLKHITLENNENKPVTNSRD 572
Query 802 TQEVKLDQGLQLIKIFKDHTSKQCILDDFDFYEDRQKRIqekkakqqqfqkqaQIWEGKA 623
TQEVKL+QGLQ++KIFK+H SK CILDDF FYE RQK I ++ K +Q Q Q Q+WEGK
Sbjct 573 TQEVKLEQGLQMLKIFKEHCSKTCILDDFAFYESRQKTI--QEKKAKQQQFQKQVWEGKP 630
Query 622 TDEKNKESTNGEVKAQQSTELASSDLNKEA 533
TDEK NGE+K Q+S E+A SDL KE+
Sbjct 631 TDEK-----NGELKPQKSLEVA-SDLIKES 654
>ref|XP_002304850.1| predicted protein [Populus trichocarpa]
gb|EEE79829.1| predicted protein [Populus trichocarpa]
Length=522
Score = 454 bits (1168), Expect = 1e-151
Identities = 223/309 (72%), Positives = 259/309 (84%), Gaps = 5/309 (2%)
Frame = -3
Query 1522 GQYGNTYRSGVGYGANSYDTRTNGRGWMSVDSKYKPRGRGNGPYNYANESVDGLNELNRG 1343
GQYGN ++SG+G+G+ Y+ NG GW+++DSKYKP+GRGNG + Y N+S+DGLNELNRG
Sbjct 210 GQYGNGFKSGMGFGSGGYNAGINGHGWLAIDSKYKPKGRGNGYFGYHNDSIDGLNELNRG 269
Query 1342 PRAKSS-KNQKGFTPVSLAVKGQSNISLTGTNDSDDKILSLIPDREQYNLPDFPETYANA 1166
PRAK KNQKGF P ++AVKGQS + + TN + +++PDREQYN DFPE Y NA
Sbjct 270 PRAKGYFKNQKGFVPATVAVKGQS-VPSSDTNVEEKDKTTVVPDREQYNKADFPEEYDNA 328
Query 1165 KFFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAYHEAQQKPGGCPIFLFFSVNTSGQF 986
KFFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAY EA+QK GGCP+FL FSVNTSGQF
Sbjct 329 KFFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAYQEAEQKSGGCPVFLLFSVNTSGQF 388
Query 985 VGAAEMTGPVDFNKNVEYWQQDKWVGCFPVKWHIVKDVPNSLLKHITLENNENKPVTNSR 806
VG AEMTG VDF+K+VEYWQQDKW G FPVKWHIVKDVPNS LKHITLENNENKPVTNSR
Sbjct 389 VGLAEMTGRVDFDKSVEYWQQDKWTGYFPVKWHIVKDVPNSFLKHITLENNENKPVTNSR 448
Query 805 DTQEVKLDQGLQLIKIFKDHTSKQCILDDFDFYEDRQKRIqekkakqqqfqkqaQIWEGK 626
DTQEVKL+QGL+LIKIFKDH+SK CILDDF FYEDR+K I ++ K +Q Q + Q+W+GK
Sbjct 449 DTQEVKLEQGLKLIKIFKDHSSKTCILDDFVFYEDREKMI--QEKKAKQQQLKKQVWDGK 506
Query 625 ATDEKNKES 599
+EK KES
Sbjct 507 PCEEK-KES 514
>ref|XP_003629923.1| YTH domain family protein [Medicago truncatula]
gb|AET04399.1| YTH domain family protein [Medicago truncatula]
Length=677
Score = 451 bits (1159), Expect = 4e-148
Identities = 222/339 (65%), Positives = 263/339 (78%), Gaps = 10/339 (3%)
Frame = -3
Query 1522 GQYGNTYRSGVGYGANSYDTRTNGRGWMSVDSKYKPRGRGNGPYNYANESVDGLNELNRG 1343
GQYGN+ RSG+GYG + YD+RTNGR W++VD+KYK RGR G + Y NE+ DGLNELNRG
Sbjct 305 GQYGNSVRSGMGYGTHGYDSRTNGRAWLAVDNKYKTRGRNGGYFGYGNENTDGLNELNRG 364
Query 1342 PRAKSSKNQKGFTPVSLAVKGQSNISLTGTNDSDDKILSLIPDREQYNLPDFPETYANAK 1163
PRAK KNQK F P L VKGQ N+ + ++ +K S PDREQYN DFPE Y +AK
Sbjct 365 PRAKGGKNQKVFVPTVLTVKGQ-NVPVNTVDEEKEKN-STTPDREQYNKADFPEEYTDAK 422
Query 1162 FFIIKSYSEDDVHKCIKYNVWASTPNGNKKLDAAYHEAQQKPGGCPIFLFFSVNTSGQFV 983
FF+IKSYSEDD+HK IKYNVWAST NGNKKLDAAY EAQQK GGCPIFL FSVNTSGQFV
Sbjct 423 FFVIKSYSEDDIHKSIKYNVWASTQNGNKKLDAAYQEAQQKSGGCPIFLLFSVNTSGQFV 482
Query 982 GAAEMTGPVDFNKNVEYWQQDKWVGCFPVKWHIVKDVPNSLLKHITLENNENKPVTNSRD 803
G AEMTGPVDFNK++EYWQQDKW+GCFP+KWHIVKDVPN++L+HITLENNENKPVTNSRD
Sbjct 483 GLAEMTGPVDFNKSLEYWQQDKWMGCFPLKWHIVKDVPNNVLRHITLENNENKPVTNSRD 542
Query 802 TQEVKLDQGLQLIKIFKDHTSKQCILDDFDFYEDRQKRI----qekkakqqqfqkqaQIW 635
TQE+ L+ GL+L+KIFK+++SK CILDDF FYE RQK I +++ +Q +W
Sbjct 543 TQEIMLEPGLKLLKIFKEYSSKTCILDDFGFYEGRQKTILEKKAKQQFPKQARLSTIPVW 602
Query 634 EGKATDEKNKESTNGEVKAQQSTELASSDLNKEAAQIND 518
EGK TDEK + NGE Q+S +S+L KE+ D
Sbjct 603 EGKPTDEKIE--VNGETNTQKSE--VNSELLKESTLAKD 637
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 14781972760
Number of extensions: 310823389
Number of successful extensions: 779747
Number of sequences better than 1e-10: 563
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 777871
Number of HSP's successfully gapped: 565
Length of query: 1524
Length of database: 6150218869
Length adjustment: 145
Effective length of query: 1379
Effective length of database: 3551951689
Effective search space: 1289358463107
Effective search space used: 1289358463107
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 179 (73.6 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCXV5ZM01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_611
Length=1092
Score E
Sequences producing significant alignments: (Bits) Value
dbj|BAH66920.1| metal transporter [Nicotiana tabacum] 334 2e-109
ref|XP_003601469.1| Zinc transporter [Medicago truncatula] >g... 331 1e-107
ref|XP_003601470.1| Zinc transporter [Medicago truncatula] >g... 331 1e-107
ref|NP_001236499.1| zinc transporter protein ZIP1 precursor [... 329 2e-107
ref|XP_002527722.1| zinc/iron transporter, putative [Ricinus ... 329 4e-107
ALIGNMENTS
>dbj|BAH66920.1| metal transporter [Nicotiana tabacum]
Length=339
Score = 334 bits (857), Expect = 2e-109
Identities = 183/278 (66%), Positives = 220/278 (79%), Gaps = 7/278 (3%)
Frame = +1
Query 4 ALTPAKEFFFVLKAFAAGVILATGFIHVLPDAFESLTSSCIGEIPWGDFPLTGFIAMMAA 183
AL+P K FFF++KAFAAGVILATGFIHVLPDAFESLTS C+ E PWG+FP +GFIAM++A
Sbjct 68 ALSPEKNFFFIIKAFAAGVILATGFIHVLPDAFESLTSPCLKENPWGNFPFSGFIAMVSA 127
Query 184 IGALLVDTYAHSYYKRREATIVKL-GSGGNQGDAMLPVhthtthghahgtVSDSEETELL 360
+G L+VDTYA SY+ + T L G++G A+ H H ++ +ELL
Sbjct 128 MGTLMVDTYATSYFSNKNDTKNGLVAQSGDEGGAI------HVHSHGSASLMGDSSSELL 181
Query 361 RHRVISQVLELGIVVHSVIIGIALGASQSPETIRPLIAALSFHQLFEGIGLGGCITQAKF 540
R+RV+SQVLE+GI+VHSVIIGIALGAS+SP+TIRPL+AAL+FHQ FEG+GLGGCI QAKF
Sbjct 182 RYRVVSQVLEMGIIVHSVIIGIALGASESPKTIRPLVAALTFHQFFEGMGLGGCIAQAKF 241
Query 541 KARTVAIMTASFSLTTPigiaigigiaNFYSETSPTALIVQGAFNSASAGILVYMALVDL 720
K R VAIM FSLTTP+GIAIG+GI N Y E SPTALIV+G FNSASAGIL+YMALVD
Sbjct 242 KTRAVAIMALFFSLTTPVGIAIGLGITNVYDENSPTALIVEGVFNSASAGILIYMALVDF 301
Query 721 LSADFMSSRMQNSSKLQLKSTFALLLGAGCMSLLAKWA 834
L+ADFM RMQ + KLQL + +LLLGAG M+L+AKWA
Sbjct 302 LAADFMHPRMQGNGKLQLGANISLLLGAGLMALIAKWA 339
>ref|XP_003601469.1| Zinc transporter [Medicago truncatula]
gb|AES71720.1| Zinc transporter [Medicago truncatula]
Length=372
Score = 331 bits (848), Expect = 1e-107
Identities = 182/282 (65%), Positives = 225/282 (80%), Gaps = 5/282 (2%)
Frame = +1
Query 4 ALTPAKEFFFVLKAFAAGVILATGFIHVLPDAFESLTSSCIGEIPWGDFPLTGFIAMMAA 183
AL+P K+ FF++KAFAAGVIL+TGFIHVLPDAFE+LTS C+ E PWGDFP TGF+AM A
Sbjct 91 ALSPEKDIFFIIKAFAAGVILSTGFIHVLPDAFENLTSPCLNEHPWGDFPFTGFVAMCTA 150
Query 184 IGALLVDTYAHSYY-----KRREATIVKLGSGGNQGDAMLPVhthtthghahgtVSDSEE 348
+G L+VDTYA +Y+ KR A + + + + VH H +H HAHG +S +
Sbjct 151 MGTLMVDTYATAYFQNHYSKRAPAQVESQTTPDVENEEHTHVHAHASHSHAHGHISFDQS 210
Query 349 TELLRHRVISQVLELGIVVHSVIIGIALGASQSPETIRPLIAALSFHQLFEGIGLGGCIT 528
+ELLRHRVISQVLELGI+VHSVIIGI+LGAS+SP+TIRPL+AAL+FHQ FEG+GLG CIT
Sbjct 211 SELLRHRVISQVLELGIIVHSVIIGISLGASESPKTIRPLVAALTFHQFFEGMGLGSCIT 270
Query 529 QAKFKARTVAIMTASFSLTTPigiaigigiaNFYSETSPTALIVQGAFNSASAGILVYMA 708
QA FK+ ++ IM F+LTTP+GI IG+GI+N Y E SPTALI +G FN+ASAGIL+YMA
Sbjct 271 QANFKSLSITIMGLFFALTTPVGIGIGLGISNVYDENSPTALIFEGIFNAASAGILIYMA 330
Query 709 LVDLLSADFMSSRMQNSSKLQLKSTFALLLGAGCMSLLAKWA 834
LVDLL+ADFM+ RMQ + +LQL S +LLLGAGCMSL+AKWA
Sbjct 331 LVDLLAADFMNPRMQKNGRLQLGSNISLLLGAGCMSLIAKWA 372
>ref|XP_003601470.1| Zinc transporter [Medicago truncatula]
gb|AES71721.1| Zinc transporter [Medicago truncatula]
Length=377
Score = 331 bits (848), Expect = 1e-107
Identities = 182/282 (65%), Positives = 225/282 (80%), Gaps = 5/282 (2%)
Frame = +1
Query 4 ALTPAKEFFFVLKAFAAGVILATGFIHVLPDAFESLTSSCIGEIPWGDFPLTGFIAMMAA 183
AL+P K+ FF++KAFAAGVIL+TGFIHVLPDAFE+LTS C+ E PWGDFP TGF+AM A
Sbjct 96 ALSPEKDIFFIIKAFAAGVILSTGFIHVLPDAFENLTSPCLNEHPWGDFPFTGFVAMCTA 155
Query 184 IGALLVDTYAHSYY-----KRREATIVKLGSGGNQGDAMLPVhthtthghahgtVSDSEE 348
+G L+VDTYA +Y+ KR A + + + + VH H +H HAHG +S +
Sbjct 156 MGTLMVDTYATAYFQNHYSKRAPAQVESQTTPDVENEEHTHVHAHASHSHAHGHISFDQS 215
Query 349 TELLRHRVISQVLELGIVVHSVIIGIALGASQSPETIRPLIAALSFHQLFEGIGLGGCIT 528
+ELLRHRVISQVLELGI+VHSVIIGI+LGAS+SP+TIRPL+AAL+FHQ FEG+GLG CIT
Sbjct 216 SELLRHRVISQVLELGIIVHSVIIGISLGASESPKTIRPLVAALTFHQFFEGMGLGSCIT 275
Query 529 QAKFKARTVAIMTASFSLTTPigiaigigiaNFYSETSPTALIVQGAFNSASAGILVYMA 708
QA FK+ ++ IM F+LTTP+GI IG+GI+N Y E SPTALI +G FN+ASAGIL+YMA
Sbjct 276 QANFKSLSITIMGLFFALTTPVGIGIGLGISNVYDENSPTALIFEGIFNAASAGILIYMA 335
Query 709 LVDLLSADFMSSRMQNSSKLQLKSTFALLLGAGCMSLLAKWA 834
LVDLL+ADFM+ RMQ + +LQL S +LLLGAGCMSL+AKWA
Sbjct 336 LVDLLAADFMNPRMQKNGRLQLGSNISLLLGAGCMSLIAKWA 377
>ref|NP_001236499.1| zinc transporter protein ZIP1 precursor [Glycine max]
gb|AAK37761.1| zinc transporter protein ZIP1 [Glycine max]
Length=354
Score = 329 bits (844), Expect = 2e-107
Identities = 181/282 (64%), Positives = 221/282 (78%), Gaps = 6/282 (2%)
Frame = +1
Query 4 ALTPAKEFFFVLKAFAAGVILATGFIHVLPDAFESLTSSCIGEIPWGDFPLTGFIAMMAA 183
AL+P K+ FF++KAFAAGVIL+TGFIHVLPDAFE+LTS C+ E PWG+FP TGF+AM A
Sbjct 74 ALSPEKDTFFIIKAFAAGVILSTGFIHVLPDAFENLTSPCLKEHPWGEFPFTGFVAMCTA 133
Query 184 IGALLVDTYAHSYYKRR-----EATIVKLGSGGNQGDAMLPVhthtthghahgtVSDSEE 348
+G L+VDTYA +Y+K+ EAT V+ SG ++G L H H H H D +
Sbjct 134 MGTLMVDTYATAYFKKHHHSQDEATDVEKESG-HEGHVHLHTHATHGHAHGHVPTDDDQS 192
Query 349 TELLRHRVISQVLELGIVVHSVIIGIALGASQSPETIRPLIAALSFHQLFEGIGLGGCIT 528
+ELLRHRVISQVLE+GI+VHS+IIGI+LGAS+SP+TIRPL+AAL FHQ FEG+GLG CIT
Sbjct 193 SELLRHRVISQVLEVGIIVHSIIIGISLGASESPKTIRPLMAALIFHQFFEGMGLGSCIT 252
Query 529 QAKFKARTVAIMTASFSLTTPigiaigigiaNFYSETSPTALIVQGAFNSASAGILVYMA 708
QA FK ++ +M F+LTTP+GI IGIGI Y E SPTALIV+G FN+ASAGIL+YMA
Sbjct 253 QANFKKLSITLMGLVFALTTPMGIGIGIGITKVYDENSPTALIVEGIFNAASAGILIYMA 312
Query 709 LVDLLSADFMSSRMQNSSKLQLKSTFALLLGAGCMSLLAKWA 834
LVDLL+ADFM+ RMQ S L+L + +LLLGAGCMSLLAKWA
Sbjct 313 LVDLLAADFMNPRMQKSGSLRLGANLSLLLGAGCMSLLAKWA 354
>ref|XP_002527722.1| zinc/iron transporter, putative [Ricinus communis]
gb|EEF34635.1| zinc/iron transporter, putative [Ricinus communis]
Length=355
Score = 329 bits (843), Expect = 4e-107
Identities = 186/287 (65%), Positives = 222/287 (77%), Gaps = 10/287 (3%)
Frame = +1
Query 4 ALTPAKEFFFVLKAFAAGVILATGFIHVLPDAFESLTSSCIGEIPWGDFPLTGFIAMMAA 183
AL+P K FF++K+FAAGVIL+TGFIH+LPDAFESLTS C+ E PWG FP TGFIAM++A
Sbjct 69 ALSPEKNVFFMIKSFAAGVILSTGFIHILPDAFESLTSPCLDENPWGKFPFTGFIAMVSA 128
Query 184 IGALLVDTYAHSYYKR---REATIVKLG-------SGGNQGDAMLPVhthtthghahgtV 333
IG L+VDTYA SY+ + R+A G +GG + H H H TV
Sbjct 129 IGTLMVDTYATSYFNKSNLRKAQSAVTGDEEKSVDNGGAHEHMHVHTHATHGHSHGRVTV 188
Query 334 SDSEETELLRHRVISQVLELGIVVHSVIIGIALGASQSPETIRPLIAALSFHQLFEGIGL 513
DS ++LLRHRVISQVLELGIVVHSVIIGI+LGAS+SP+TIRPL+AAL+FHQ FEG+GL
Sbjct 189 QDSVPSDLLRHRVISQVLELGIVVHSVIIGISLGASESPKTIRPLVAALTFHQFFEGMGL 248
Query 514 GGCITQAKFKARTVAIMTASFSLTTPigiaigigiaNFYSETSPTALIVQGAFNSASAGI 693
GGCI+QA FK R V M F+LTTP GIAIGIGI+N Y+E SPTALIV+G FNSASAGI
Sbjct 249 GGCISQANFKTRAVTTMVLFFALTTPAGIAIGIGISNVYNENSPTALIVEGVFNSASAGI 308
Query 694 LVYMALVDLLSADFMSSRMQNSSKLQLKSTFALLLGAGCMSLLAKWA 834
L+YMALVDLL+ADFM+ ++Q S+KLQ +LLLGAGCM+LLAKWA
Sbjct 309 LIYMALVDLLAADFMNPKVQASAKLQFGVNVSLLLGAGCMALLAKWA 355
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 563864443
Number of extensions: 11868017
Number of successful extensions: 28384
Number of sequences better than 1e-10: 22
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 28308
Number of HSP's successfully gapped: 26
Length of query: 1092
Length of database: 6150218869
Length adjustment: 142
Effective length of query: 950
Effective length of database: 3605708941
Effective search space: 800467384902
Effective search space used: 800467384902
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 177 (72.8 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDADHZR01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_6408
Length=599
Score E
Sequences producing significant alignments: (Bits) Value
emb|CBI36799.3| unnamed protein product [Vitis vinifera] 110 7e-26
ref|XP_002284549.1| PREDICTED: protein AUXIN RESPONSE 4 [Viti... 110 1e-25
ref|XP_002512384.1| conserved hypothetical protein [Ricinus c... 107 2e-24
ref|XP_002319010.1| predicted protein [Populus trichocarpa] >... 104 2e-23
ref|XP_003626280.1| Protein AUXIN RESPONSE [Medicago truncatu... 97.8 8e-21
ALIGNMENTS
>emb|CBI36799.3| unnamed protein product [Vitis vinifera]
Length=366
Score = 110 bits (276), Expect = 7e-26
Identities = 57/103 (55%), Positives = 68/103 (66%), Gaps = 9/103 (9%)
Frame = +1
Query 1 EADAHRILLKGRDGVKAVVGMGKKLNLSFDLSEWGGRDEVKGLPVLVL---------SSG 153
+ +AHR+LLKGRDG +AVVG KKLN SFDL EWG + +KGLPV VL S G
Sbjct 204 DVEAHRVLLKGRDGARAVVGTWKKLNSSFDLQEWGDSEGLKGLPVQVLWSSGWSKEWSEG 263
Query 154 ERAFGDVFPMAMFGTHSGGVWPGEDSAEEVAKGVYEFVSSLPK 282
R D P A F THSG WP ED+A+E+A+ + FVSSLPK
Sbjct 264 GRKVADALPHATFITHSGSRWPQEDAADELAENILRFVSSLPK 306
>ref|XP_002284549.1| PREDICTED: protein AUXIN RESPONSE 4 [Vitis vinifera]
Length=474
Score = 110 bits (276), Expect = 1e-25
Identities = 57/103 (55%), Positives = 68/103 (66%), Gaps = 9/103 (9%)
Frame = +1
Query 1 EADAHRILLKGRDGVKAVVGMGKKLNLSFDLSEWGGRDEVKGLPVLVL---------SSG 153
+ +AHR+LLKGRDG +AVVG KKLN SFDL EWG + +KGLPV VL S G
Sbjct 312 DVEAHRVLLKGRDGARAVVGTWKKLNSSFDLQEWGDSEGLKGLPVQVLWSSGWSKEWSEG 371
Query 154 ERAFGDVFPMAMFGTHSGGVWPGEDSAEEVAKGVYEFVSSLPK 282
R D P A F THSG WP ED+A+E+A+ + FVSSLPK
Sbjct 372 GRKVADALPHATFITHSGSRWPQEDAADELAENILRFVSSLPK 414
>ref|XP_002512384.1| conserved hypothetical protein [Ricinus communis]
gb|EEF49836.1| conserved hypothetical protein [Ricinus communis]
Length=473
Score = 107 bits (267), Expect = 2e-24
Identities = 52/108 (48%), Positives = 71/108 (66%), Gaps = 9/108 (8%)
Frame = +1
Query 1 EADAHRILLKGRDGVKAVVGMGKKLNLSFDLSEWGGRDEVKGLPVLVLSSGE-------- 156
+ +AHR++LKGRDG +AVVG GKKLN SF + +WGG D ++GLP+ V+ S
Sbjct 320 DLEAHRVILKGRDGRRAVVGTGKKLNSSFSIPQWGGSDGIRGLPIQVIWSNSWSIEWSEE 379
Query 157 -RAFGDVFPMAMFGTHSGGVWPGEDSAEEVAKGVYEFVSSLPKVDKPI 297
R + P A F HSGG W ED+A+EVA+ + +FVSSLPK D+ +
Sbjct 380 GRRVAEALPHAKFVLHSGGRWSQEDAADEVAENIIKFVSSLPKSDRKV 427
>ref|XP_002319010.1| predicted protein [Populus trichocarpa]
gb|EEE94933.1| predicted protein [Populus trichocarpa]
Length=460
Score = 104 bits (260), Expect = 2e-23
Identities = 52/100 (52%), Positives = 68/100 (68%), Gaps = 9/100 (9%)
Frame = +1
Query 10 AHRILLKGRDGVKAVVGMGKKLNLSFDLSEWGGRDEVKGLPVLVL---------SSGERA 162
AHR++L GRDG +AVV GKKLN SFD++EWGG D VKG+P+ V+ S R
Sbjct 297 AHRMMLNGRDGRRAVVATGKKLNSSFDIAEWGGLDGVKGIPMQVVWCSGWSKEWSEEGRK 356
Query 163 FGDVFPMAMFGTHSGGVWPGEDSAEEVAKGVYEFVSSLPK 282
D P A F TH+GG WP E++A+E+A+ + +FVSSLPK
Sbjct 357 VADALPQAKFVTHTGGRWPQENAADELAENIVKFVSSLPK 396
>ref|XP_003626280.1| Protein AUXIN RESPONSE [Medicago truncatula]
gb|AES82498.1| Protein AUXIN RESPONSE [Medicago truncatula]
Length=494
Score = 97.8 bits (242), Expect = 8e-21
Identities = 53/109 (49%), Positives = 68/109 (62%), Gaps = 11/109 (10%)
Frame = +1
Query 1 EADAHRILLKGRDGVKAVVGMGKKLNLSFDLSEWGGRDEVKGLPVLVL----------SS 150
+ADAHR+LLK DG KAVV +GK LN SFDL+EWG D +K +P+ ++ S
Sbjct 328 DADAHRVLLKSGDGRKAVVAIGKNLNSSFDLTEWGCSDRLKDMPMQLIWSSDWSEEWSSE 387
Query 151 GERAFGDVFPMAMFGTHSGGVWPGEDSAEEVAKGVYEFVSSLPKVDKPI 297
G R G P A F THSGG W ED A E+A+ + +FVSSLPK + +
Sbjct 388 GNRVAG-ALPRAKFVTHSGGRWAQEDVAVEIAEKISQFVSSLPKTVRKV 435
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 260777034
Number of extensions: 5080677
Number of successful extensions: 15802
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 15755
Number of HSP's successfully gapped: 0
Length of query: 599
Length of database: 6150218869
Length adjustment: 133
Effective length of query: 466
Effective length of database: 3766980697
Effective search space: 248620726002
Effective search space used: 248620726002
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 173 (71.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDBNWC1016
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_6864
Length=580
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002274668.1| PREDICTED: cysteine-rich repeat secretory... 144 2e-39
ref|XP_002327834.1| predicted protein [Populus trichocarpa] >... 143 7e-39
emb|CAN73038.1| hypothetical protein VITISV_044348 [Vitis vin... 142 2e-38
ref|XP_002327925.1| predicted protein [Populus trichocarpa] >... 139 1e-37
ref|XP_002327923.1| predicted protein [Populus trichocarpa] >... 138 3e-37
ALIGNMENTS
>ref|XP_002274668.1| PREDICTED: cysteine-rich repeat secretory protein 38-like [Vitis
vinifera]
Length=242
Score = 144 bits (363), Expect = 2e-39
Identities = 77/176 (44%), Positives = 107/176 (61%), Gaps = 7/176 (4%)
Frame = +2
Query 2 GQGPDTTYGLALCRGDVSPDYCDFCLYDARCKITQRCY-TKSAIVMYDYCQVKYSNENFF 178
G+ D GLALCRGDVS C C+ ++ +I +RC K+AI+ YDYC +KYSN NFF
Sbjct 70 GENNDQVNGLALCRGDVSNTDCKACITESSSEIRKRCPDNKAAIIWYDYCLLKYSNVNFF 129
Query 179 GKIDHTNDNILINVYNVSANQTKQFKDASIGLLNKLSNQAAHSKSLFAKGDKLFDKKENV 358
G++DH N + N+ NVS F + LL+ L+ QA ++ ++A G+ + +E+
Sbjct 130 GQVDHQNMFYMWNLNNVS--DPDSFNQKTKELLSNLAQQAFNAMKMYATGE--LELEESE 185
Query 359 SIYGMVQCTQDLSNDSCKKCLGDVVKKLPIYKDEIYSVGARVFGSSCTVRYEEYGF 526
+YG+ QCT+DLS+ CKKCL D + KLP D G RV G SC +RYE Y F
Sbjct 186 KLYGLTQCTRDLSSSDCKKCLDDAISKLPNCCDG--KEGGRVVGGSCNIRYEIYPF 239
>ref|XP_002327834.1| predicted protein [Populus trichocarpa]
gb|EEE75622.1| predicted protein [Populus trichocarpa]
Length=242
Score = 143 bits (360), Expect = 7e-39
Identities = 80/176 (45%), Positives = 108/176 (61%), Gaps = 7/176 (4%)
Frame = +2
Query 2 GQGPDTTYGLALCRGDVSPDYCDFCLYDARCKITQRC-YTKSAIVMYDYCQVKYSNENFF 178
G PD TYGLALCRGDVS C C+ +A +I +RC Y K+AI+ YD C +KYSN FF
Sbjct 70 GHTPDQTYGLALCRGDVSTSDCKTCVVEASSEIRKRCPYNKAAIIWYDNCLLKYSNNAFF 129
Query 179 GKIDHTNDNILINVYNVSANQTKQFKDASIGLLNKLSNQAAHSKSLFAKGDKLFDKKENV 358
G+ID+ N + NV+ VS + F + + LL++L+N+A + LFA G++ K +
Sbjct 130 GQIDNGNKFYMWNVHVVS--EPAPFNEKTKELLSQLANEAQATPKLFATGERELGK--ST 185
Query 359 SIYGMVQCTQDLSNDSCKKCLGDVVKKLPIYKDEIYSVGARVFGSSCTVRYEEYGF 526
+YG+VQCT DLS+ CKKCL ++ +LPI D G RV SC YE Y F
Sbjct 186 KLYGLVQCTGDLSSAVCKKCLDGIIGELPICCDG--KQGGRVVSGSCNFIYEIYPF 239
>emb|CAN73038.1| hypothetical protein VITISV_044348 [Vitis vinifera]
Length=243
Score = 142 bits (357), Expect = 2e-38
Identities = 84/177 (47%), Positives = 106/177 (60%), Gaps = 9/177 (5%)
Frame = +2
Query 2 GQGPDTTYGLALCRGDVSPDYCDFCLYDARCKITQRC-YTKSAIVMYDYCQVKYSNENFF 178
GQG D+ GLALCRGDVS C C+ +A ++ RC Y K AI+ YD C YSN FF
Sbjct 69 GQGQDSVNGLALCRGDVSSRDCKTCVTEATKELHNRCPYNKGAIIWYDNCLFNYSNVKFF 128
Query 179 GKIDHTNDNILINVYNV-SANQTKQFKDASIGLLNKLSNQAAHSKSLFAKGDKLFDKKEN 355
G+ID+ N VYNV SA+ F + LL+ LSN+A S LFA G+ + + E+
Sbjct 129 GEIDNKNK---FYVYNVQSADDPTSFNVKTKELLSSLSNKAYGSPMLFATGELVLE--ES 183
Query 356 VSIYGMVQCTQDLSNDSCKKCLGDVVKKLPIYKDEIYSVGARVFGSSCTVRYEEYGF 526
+ +YG+ QCT+DLS+ CKK L D V +LP Y D G RV G SC VRYE Y F
Sbjct 184 MKLYGLAQCTRDLSSLDCKKSLDDAVSELPSYCDG--KRGGRVVGGSCNVRYELYPF 238
>ref|XP_002327925.1| predicted protein [Populus trichocarpa]
gb|EEE75713.1| predicted protein [Populus trichocarpa]
Length=242
Score = 139 bits (351), Expect = 1e-37
Identities = 78/176 (44%), Positives = 106/176 (60%), Gaps = 7/176 (4%)
Frame = +2
Query 2 GQGPDTTYGLALCRGDVSPDYCDFCLYDARCKITQRC-YTKSAIVMYDYCQVKYSNENFF 178
G PD TYGLALCRGDVS C C+++A +I +RC Y K+AI+ YD C +KYSN FF
Sbjct 70 GHTPDQTYGLALCRGDVSTSDCKTCVFEASSEIRKRCPYNKAAIIWYDNCLLKYSNTGFF 129
Query 179 GKIDHTNDNILINVYNVSANQTKQFKDASIGLLNKLSNQAAHSKSLFAKGDKLFDKKENV 358
G++D N + NV+ VS + F + LL++L+N+A + LFA G++ K +
Sbjct 130 GQVDTGNKFYMWNVHVVS--KPAPFNKKTKELLSQLANEAQATPKLFATGERELGK--ST 185
Query 359 SIYGMVQCTQDLSNDSCKKCLGDVVKKLPIYKDEIYSVGARVFGSSCTVRYEEYGF 526
+YG+VQCT DLS+ CKKCL ++ +LP D G RV SC YE Y F
Sbjct 186 KLYGLVQCTGDLSSAVCKKCLDGIIGELPSCCDG--KQGGRVVSGSCNFIYELYPF 239
>ref|XP_002327923.1| predicted protein [Populus trichocarpa]
gb|EEE75711.1| predicted protein [Populus trichocarpa]
Length=214
Score = 138 bits (347), Expect = 3e-37
Identities = 78/176 (44%), Positives = 105/176 (60%), Gaps = 7/176 (4%)
Frame = +2
Query 2 GQGPDTTYGLALCRGDVSPDYCDFCLYDARCKITQRC-YTKSAIVMYDYCQVKYSNENFF 178
G PD TYGLALCRGDVS C C+ +A +I +RC Y K+AI+ YD C +KYSN FF
Sbjct 42 GHTPDQTYGLALCRGDVSTSDCKTCVVEASSEIRKRCPYNKAAIIWYDNCLLKYSNTGFF 101
Query 179 GKIDHTNDNILINVYNVSANQTKQFKDASIGLLNKLSNQAAHSKSLFAKGDKLFDKKENV 358
G++D N + NV+ VS + F + LL++L+N+A + LFA G++ K +
Sbjct 102 GQVDTGNKFYMWNVHVVS--KPAPFNKKTKELLSQLANEAQATPKLFATGERELGK--ST 157
Query 359 SIYGMVQCTQDLSNDSCKKCLGDVVKKLPIYKDEIYSVGARVFGSSCTVRYEEYGF 526
+YG+VQCT DLS+ CKKCL ++ +LP D G RV SC YE Y F
Sbjct 158 KLYGLVQCTGDLSSAVCKKCLDGIIGELPSCCDG--KQGGRVVSGSCNFIYEIYPF 211
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 5943358530
Number of extensions: 124046776
Number of successful extensions: 307390
Number of sequences better than 1e-10: 642
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 304564
Number of HSP's successfully gapped: 664
Length of query: 580
Length of database: 6150218869
Length adjustment: 133
Effective length of query: 447
Effective length of database: 3766980697
Effective search space: 226018841820
Effective search space used: 226018841820
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 173 (71.2 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCYFCT0012
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_710
Length=1065
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002264621.1| PREDICTED: zinc transporter 5 isoform 1 [... 288 3e-91
dbj|BAH66920.1| metal transporter [Nicotiana tabacum] 285 2e-90
emb|CAN74488.1| hypothetical protein VITISV_029272 [Vitis vin... 286 2e-90
ref|XP_002527722.1| zinc/iron transporter, putative [Ricinus ... 281 1e-88
ref|XP_002307860.1| ZIP transporter [Populus trichocarpa] >gb... 278 2e-87
ALIGNMENTS
>ref|XP_002264621.1| PREDICTED: zinc transporter 5 isoform 1 [Vitis vinifera]
Length=351
Score = 288 bits (736), Expect = 3e-91
Identities = 156/226 (69%), Positives = 182/226 (81%), Gaps = 3/226 (1%)
Frame = -1
Query 1065 AAIGTLMVDTYATSYYGRRSGVKKEAAVGNGD---EEVGVIPVHNhaahghahgAVSLEP 895
+AIGTLMVD ATSYY R K + AVG+ + E G + VH H HGHAHG+ S
Sbjct 126 SAIGTLMVDACATSYYSRSHFKKAQQAVGDEEKAGEHEGHVHVHTHGTHGHAHGSASSAE 185
Query 894 GSGETELLRHRIISQVLELGIIVHSVIIGIALGASQNPKTIRPLIAALTFHQFFEGIGLG 715
G EL+RHR+ISQVLELGI+VHSVIIGI+LGAS++PKTI+PL+AALTFHQFFEG+GLG
Sbjct 186 EMGSAELIRHRVISQVLELGIVVHSVIIGISLGASESPKTIKPLVAALTFHQFFEGMGLG 245
Query 714 GCIAQARFNTRAVVMMAFFFSITTPSGIAIGIGISRFYSETSRNALIIEGVFNSASAGIL 535
GCI QA+F RA +MA FFS+TTP GIAIGIGIS Y E S ALI+EG+FN+ASAGIL
Sbjct 246 GCIVQAKFKLRAAAIMALFFSLTTPVGIAIGIGISNVYDENSSTALIVEGIFNAASAGIL 305
Query 534 IYMALVDLLAADFMSPKMQNNGKLQLLANASLLVGAGCMSLLAKWA 397
+YMALVDLLAADFM+P+MQ NG+LQ+ AN SLLVGAGCMSLLAKWA
Sbjct 306 VYMALVDLLAADFMNPRMQGNGRLQVGANISLLVGAGCMSLLAKWA 351
>dbj|BAH66920.1| metal transporter [Nicotiana tabacum]
Length=339
Score = 285 bits (730), Expect = 2e-90
Identities = 150/223 (67%), Positives = 177/223 (79%), Gaps = 9/223 (4%)
Frame = -1
Query 1065 AAIGTLMVDTYATSYYGRRSGVKKEAAVGNGDEEVGVIPVHNhaahghahgAVSLEPGSG 886
+A+GTLMVDTYATSY+ ++ K +GDE G I VH+H + G
Sbjct 126 SAMGTLMVDTYATSYFSNKNDTKNGLVAQSGDEG-GAIHVHSHGSASLM--------GDS 176
Query 885 ETELLRHRIISQVLELGIIVHSVIIGIALGASQNPKTIRPLIAALTFHQFFEGIGLGGCI 706
+ELLR+R++SQVLE+GIIVHSVIIGIALGAS++PKTIRPL+AALTFHQFFEG+GLGGCI
Sbjct 177 SSELLRYRVVSQVLEMGIIVHSVIIGIALGASESPKTIRPLVAALTFHQFFEGMGLGGCI 236
Query 705 AQARFNTRAVVMMAFFFSITTPSGIAIGIGISRFYSETSRNALIIEGVFNSASAGILIYM 526
AQA+F TRAV +MA FFS+TTP GIAIG+GI+ Y E S ALI+EGVFNSASAGILIYM
Sbjct 237 AQAKFKTRAVAIMALFFSLTTPVGIAIGLGITNVYDENSPTALIVEGVFNSASAGILIYM 296
Query 525 ALVDLLAADFMSPKMQNNGKLQLLANASLLVGAGCMSLLAKWA 397
ALVD LAADFM P+MQ NGKLQL AN SLL+GAG M+L+AKWA
Sbjct 297 ALVDFLAADFMHPRMQGNGKLQLGANISLLLGAGLMALIAKWA 339
>emb|CAN74488.1| hypothetical protein VITISV_029272 [Vitis vinifera]
Length=351
Score = 286 bits (731), Expect = 2e-90
Identities = 157/226 (69%), Positives = 182/226 (81%), Gaps = 3/226 (1%)
Frame = -1
Query 1065 AAIGTLMVDTYATSYYGRRSGVKKEAAVGNGD---EEVGVIPVHNhaahghahgAVSLEP 895
+AIGTLMVD ATSYY K + AVG+ + E G + VH HA HGHAHG+ S
Sbjct 126 SAIGTLMVDACATSYYSXSHFKKAQQAVGDEEKAGEHEGHVHVHTHATHGHAHGSASSAE 185
Query 894 GSGETELLRHRIISQVLELGIIVHSVIIGIALGASQNPKTIRPLIAALTFHQFFEGIGLG 715
G EL+RHR+ISQVLELGI+VHSVIIGI+LGAS++PKTI+PL+AALTFHQFFEG+GLG
Sbjct 186 EMGSAELIRHRVISQVLELGIVVHSVIIGISLGASESPKTIKPLVAALTFHQFFEGMGLG 245
Query 714 GCIAQARFNTRAVVMMAFFFSITTPSGIAIGIGISRFYSETSRNALIIEGVFNSASAGIL 535
GCI QA+F RA +MA FFS+TTP GIAIGIGIS Y E S ALI+EG+FN+ASAGIL
Sbjct 246 GCIVQAKFKLRAAAIMALFFSLTTPVGIAIGIGISNVYDENSSTALIVEGIFNAASAGIL 305
Query 534 IYMALVDLLAADFMSPKMQNNGKLQLLANASLLVGAGCMSLLAKWA 397
IYMALVDLLAADFM+P+MQ NG+LQ+ AN SLLVGAGCMSLLAKWA
Sbjct 306 IYMALVDLLAADFMNPRMQGNGRLQVGANISLLVGAGCMSLLAKWA 351
>ref|XP_002527722.1| zinc/iron transporter, putative [Ricinus communis]
gb|EEF34635.1| zinc/iron transporter, putative [Ricinus communis]
Length=355
Score = 281 bits (719), Expect = 1e-88
Identities = 158/231 (68%), Positives = 189/231 (82%), Gaps = 10/231 (4%)
Frame = -1
Query 1065 AAIGTLMVDTYATSYYGRRSGVKKEAAVGNGDEEVGV--------IPVHNhaahghahgA 910
+AIGTLMVDTYATSY+ + + K ++AV GDEE V + VH HA HGH+HG
Sbjct 127 SAIGTLMVDTYATSYFNKSNLRKAQSAV-TGDEEKSVDNGGAHEHMHVHTHATHGHSHGR 185
Query 909 VSLEPGSGETELLRHRIISQVLELGIIVHSVIIGIALGASQNPKTIRPLIAALTFHQFFE 730
V+++ S ++LLRHR+ISQVLELGI+VHSVIIGI+LGAS++PKTIRPL+AALTFHQFFE
Sbjct 186 VTVQD-SVPSDLLRHRVISQVLELGIVVHSVIIGISLGASESPKTIRPLVAALTFHQFFE 244
Query 729 GIGLGGCIAQARFNTRAVVMMAFFFSITTPSGIAIGIGISRFYSETSRNALIIEGVFNSA 550
G+GLGGCI+QA F TRAV M FF++TTP+GIAIGIGIS Y+E S ALI+EGVFNSA
Sbjct 245 GMGLGGCISQANFKTRAVTTMVLFFALTTPAGIAIGIGISNVYNENSPTALIVEGVFNSA 304
Query 549 SAGILIYMALVDLLAADFMSPKMQNNGKLQLLANASLLVGAGCMSLLAKWA 397
SAGILIYMALVDLLAADFM+PK+Q + KLQ N SLL+GAGCM+LLAKWA
Sbjct 305 SAGILIYMALVDLLAADFMNPKVQASAKLQFGVNVSLLLGAGCMALLAKWA 355
>ref|XP_002307860.1| ZIP transporter [Populus trichocarpa]
gb|EEE91383.1| ZIP transporter [Populus trichocarpa]
Length=343
Score = 278 bits (710), Expect = 2e-87
Identities = 147/227 (65%), Positives = 178/227 (78%), Gaps = 5/227 (2%)
Frame = -1
Query 1065 AAIGTLMVDTYATSYYGRRSGVKKEAAVGNGDEEVGVIPVHNhaahghahgAVSL----E 898
+AIGTLMVD A+SYY R + K +GDEE + H H HA S
Sbjct 118 SAIGTLMVDCLASSYYTRLH-LNKAQPEESGDEEKAAVEAHEGHVHTHATHGHSHGLVDS 176
Query 897 PGSGETELLRHRIISQVLELGIIVHSVIIGIALGASQNPKTIRPLIAALTFHQFFEGIGL 718
GSG ++L+RHR+I+QVLELGI+VHSVIIG++LGAS +PKTIRPL+AAL+FHQFFEG+GL
Sbjct 177 SGSGPSQLIRHRVITQVLELGIVVHSVIIGVSLGASGSPKTIRPLVAALSFHQFFEGMGL 236
Query 717 GGCIAQARFNTRAVVMMAFFFSITTPSGIAIGIGISRFYSETSRNALIIEGVFNSASAGI 538
GGCI QA+F T+ +V+MA FFS+TTP GIAIG+GIS Y+E+S NALI+EG+FN+ASAGI
Sbjct 237 GGCITQAKFKTKTIVIMALFFSLTTPVGIAIGLGISNVYNESSPNALIVEGIFNAASAGI 296
Query 537 LIYMALVDLLAADFMSPKMQNNGKLQLLANASLLVGAGCMSLLAKWA 397
LIYMALVDLLAADFM PK+Q+NG LQ N SLL+GAGCMSLLAKWA
Sbjct 297 LIYMALVDLLAADFMHPKVQSNGALQFGVNVSLLLGAGCMSLLAKWA 343
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 10945858755
Number of extensions: 235442990
Number of successful extensions: 506257
Number of sequences better than 1e-10: 772
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 504410
Number of HSP's successfully gapped: 774
Length of query: 1065
Length of database: 6150218869
Length adjustment: 142
Effective length of query: 923
Effective length of database: 3605708941
Effective search space: 768016004433
Effective search space used: 768016004433
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 177 (72.8 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDC97EC01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_7395
Length=561
Score E
Sequences producing significant alignments: (Bits) Value
emb|CAN68901.1| hypothetical protein VITISV_019988 [Vitis vin... 175 1e-52
ref|XP_002265050.1| PREDICTED: basic form of pathogenesis-rel... 174 5e-52
ref|XP_003608313.1| Pathogenesis-related protein 1A [Medicago... 173 1e-51
gb|ADD97801.1| pathogenesis-related protein 1 [Musa ABB Group] 172 2e-51
ref|XP_002313932.1| predicted protein [Populus trichocarpa] >... 169 3e-50
ALIGNMENTS
>emb|CAN68901.1| hypothetical protein VITISV_019988 [Vitis vinifera]
Length=150
Score = 175 bits (444), Expect = 1e-52
Identities = 80/152 (53%), Positives = 106/152 (70%), Gaps = 8/152 (5%)
Frame = +1
Query 25 SLTIASTMAYNYQYEFLETHNEVRANVTKEINIYLPPLVWNQTVAGYALSYAYERSEDCD 204
+L + ++ A N Q ++L HN RA V+ + P+ WN TVA YA SYA ++ DC+
Sbjct 6 ALLVHTSFAQNSQQDYLNAHNTARAQVS------VGPMTWNNTVAAYAQSYANKQIGDCN 59
Query 205 MVHSDGPYGENLTEGS-PFSATDAVRFWASEKADYNYDYNSCAEGQVCGHYTQIVWKDST 381
+VHS+GPYGENL +GS F+ T AV W +EK +Y+Y NSC G+ C HYTQ+VW++S
Sbjct 60 LVHSNGPYGENLAKGSGSFTGTAAVNLWVAEKPNYDYSSNSCVGGE-CRHYTQVVWRNSV 118
Query 382 QLGCARLMCKNGMFFMICNYYPPGNYIGERPY 477
LGCAR C NG +F+ICNYYPPGNYIG+RPY
Sbjct 119 SLGCARAQCNNGWWFIICNYYPPGNYIGQRPY 150
>ref|XP_002265050.1| PREDICTED: basic form of pathogenesis-related protein 1 [Vitis
vinifera]
emb|CBI35453.3| unnamed protein product [Vitis vinifera]
Length=161
Score = 174 bits (441), Expect = 5e-52
Identities = 79/152 (52%), Positives = 107/152 (70%), Gaps = 8/152 (5%)
Frame = +1
Query 25 SLTIASTMAYNYQYEFLETHNEVRANVTKEINIYLPPLVWNQTVAGYALSYAYERSEDCD 204
+L + ++ A N Q ++L HN RA V+ + P+ WN TVA YA SYA ++ DC+
Sbjct 17 ALLVHTSYAQNSQQDYLNAHNTARAQVS------VGPMTWNNTVAAYAQSYANKQIGDCN 70
Query 205 MVHSDGPYGENLTEGS-PFSATDAVRFWASEKADYNYDYNSCAEGQVCGHYTQIVWKDST 381
+VHS+GPYGENL +GS F+ T AV W +EK +Y+Y+ NSC G+ C HYTQ+VW++S
Sbjct 71 LVHSNGPYGENLAKGSGSFTGTAAVNLWVAEKPNYDYNSNSCVGGE-CRHYTQVVWRNSV 129
Query 382 QLGCARLMCKNGMFFMICNYYPPGNYIGERPY 477
LGCAR C NG +++ICNYYPPGNYIG+RPY
Sbjct 130 SLGCARAQCNNGWWYIICNYYPPGNYIGQRPY 161
>ref|XP_003608313.1| Pathogenesis-related protein 1A [Medicago truncatula]
gb|AES90510.1| Pathogenesis-related protein 1A [Medicago truncatula]
Length=159
Score = 173 bits (438), Expect = 1e-51
Identities = 79/154 (51%), Positives = 106/154 (69%), Gaps = 9/154 (6%)
Frame = +1
Query 22 FSLTIASTMAYNYQYEFLETHNEVRANVTKEINIYLPPLVWNQTVAGYALSYAYERSEDC 201
FS+ +A+N ++L+ HN+ RA V + PLVWN+T+A YA++YA + E C
Sbjct 13 FSMCCFLCLAHNAPQDYLDVHNKARAEVG------VGPLVWNETLASYAMNYAKSKHETC 66
Query 202 DMVHSDGPYGENLTEGSP--FSATDAVRFWASEKADYNYDYNSCAEGQVCGHYTQIVWKD 375
+MVHS GPYGENL EGS +A DAV+ W EKA Y+Y N+C + + C HYTQ+VW +
Sbjct 67 EMVHSQGPYGENLAEGSDPQMNAADAVKLWVDEKAFYDYGTNACVKDE-CRHYTQVVWSN 125
Query 376 STQLGCARLMCKNGMFFMICNYYPPGNYIGERPY 477
+ QLGCAR CKNG F IC+YYPPGNY+G++PY
Sbjct 126 TKQLGCARESCKNGWTFFICSYYPPGNYVGDKPY 159
>gb|ADD97801.1| pathogenesis-related protein 1 [Musa ABB Group]
Length=163
Score = 172 bits (437), Expect = 2e-51
Identities = 79/159 (50%), Positives = 102/159 (64%), Gaps = 8/159 (5%)
Frame = +1
Query 7 LFTFTFSLTIASTMAYNYQYEFLETHNEVRANVTKEINIYLPPLVWNQTVAGYALSYAYE 186
L T ++ ST+A N +F+ HN RA V + P+ W+ TVA YA +YA +
Sbjct 11 LCTVALAMACTSTLAQNSPQDFVSPHNAARAAVG------VGPVSWDSTVAAYAQNYANQ 64
Query 187 RSEDCDMVHSDGPYGENLTEGS--PFSATDAVRFWASEKADYNYDYNSCAEGQVCGHYTQ 360
R DC +VHS GPYGEN+ GS ++A DAV W SEK DY+Y+ N CA +VCGHYTQ
Sbjct 65 RKADCQLVHSGGPYGENIFSGSGRDYTAADAVNAWVSEKQDYDYNSNKCAPNKVCGHYTQ 124
Query 361 IVWKDSTQLGCARLMCKNGMFFMICNYYPPGNYIGERPY 477
+VW+ ST +GC R+ C NG F+ CNY PPGNY G+RPY
Sbjct 125 VVWRSSTAIGCGRVRCNNGGIFITCNYKPPGNYAGQRPY 163
>ref|XP_002313932.1| predicted protein [Populus trichocarpa]
gb|EEE87887.1| predicted protein [Populus trichocarpa]
Length=153
Score = 169 bits (428), Expect = 3e-50
Identities = 82/160 (51%), Positives = 108/160 (68%), Gaps = 9/160 (6%)
Frame = +1
Query 4 PLFTFTFSLTIAS-TMAYNYQYEFLETHNEVRANVTKEINIYLPPLVWNQTVAGYALSYA 180
PL + SL +A + A N Q ++L HN R+ VT + ++W+ TVA YAL+YA
Sbjct 1 PLLVYLVSLALAHPSHAQNSQQDYLNAHNAARSQVT------VANIIWDNTVAAYALNYA 54
Query 181 YERSEDCDMVHSDGPYGENLTEGS-PFSATDAVRFWASEKADYNYDYNSCAEGQVCGHYT 357
R DC++VHS+GPYGENL +GS F+ T AV W +EK Y+Y NSC GQ C HYT
Sbjct 55 NSRISDCNLVHSNGPYGENLAKGSGSFTGTAAVNLWVAEKPYYDYASNSCVGGQ-CLHYT 113
Query 358 QIVWKDSTQLGCARLMCKNGMFFMICNYYPPGNYIGERPY 477
Q+VW++S ++GCAR+ C NG +F+ CNY PPGNYIGERPY
Sbjct 114 QVVWRNSVRVGCARVKCTNGWWFVSCNYDPPGNYIGERPY 153
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 279704079
Number of extensions: 5585148
Number of successful extensions: 12574
Number of sequences better than 1e-10: 46
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 12468
Number of HSP's successfully gapped: 46
Length of query: 561
Length of database: 6150218869
Length adjustment: 132
Effective length of query: 429
Effective length of database: 3784899781
Effective search space: 208169487955
Effective search space used: 208169487955
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 172 (70.9 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDCAT9V01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_7624
Length=553
Score E
Sequences producing significant alignments: (Bits) Value
gb|ABF06706.1| UP-9A [Nicotiana tabacum] 66.2 2e-11
ref|XP_002269666.2| PREDICTED: uncharacterized protein LOC100... 64.7 8e-11
ALIGNMENTS
>gb|ABF06706.1| UP-9A [Nicotiana tabacum]
Length=117
Score = 66.2 bits (160), Expect = 2e-11
Identities = 33/45 (73%), Positives = 39/45 (87%), Gaps = 0/45 (0%)
Frame = -2
Query 399 VAEEAEERLCCQLGEFEAEAVDQAREYRARMMDLMEQLSGAKELI 265
VAEEAEERLC QLGE EAEAVDQAR YR R++ LM+QLS A++L+
Sbjct 62 VAEEAEERLCSQLGELEAEAVDQARTYRTRVIHLMDQLSLAQKLL 106
>ref|XP_002269666.2| PREDICTED: uncharacterized protein LOC100267499 [Vitis vinifera]
Length=116
Score = 64.7 bits (156), Expect = 8e-11
Identities = 33/45 (73%), Positives = 36/45 (80%), Gaps = 0/45 (0%)
Frame = -2
Query 399 VAEEAEERLCCQLGEFEAEAVDQAREYRARMMDLMEQLSGAKELI 265
VAEEAEERLC QLGE EAEAVDQAR+Y R++ LM QLS A LI
Sbjct 63 VAEEAEERLCSQLGELEAEAVDQARQYNNRIVSLMNQLSQAHRLI 107
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 206007048
Number of extensions: 3508974
Number of successful extensions: 6978
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 6978
Number of HSP's successfully gapped: 0
Length of query: 553
Length of database: 6150218869
Length adjustment: 132
Effective length of query: 421
Effective length of database: 3784899781
Effective search space: 196814788612
Effective search space used: 196814788612
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 172 (70.9 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPCYP4HY01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_772
Length=1049
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002275557.1| PREDICTED: putative expansin-B2 [Vitis vi... 265 1e-83
ref|XP_002278559.1| PREDICTED: putative expansin-B2 [Vitis vi... 261 3e-82
ref|NP_001234859.1| beta-expansin precursor [Solanum lycopers... 261 5e-82
gb|ABC47127.1| beta expansin 1 precursor [Solanum tuberosum] 258 5e-81
ref|XP_002320708.1| hypothetical protein POPTRDRAFT_572072 [P... 251 5e-78
ALIGNMENTS
>ref|XP_002275557.1| PREDICTED: putative expansin-B2 [Vitis vinifera]
emb|CBI16592.3| unnamed protein product [Vitis vinifera]
Length=273
Score = 265 bits (677), Expect = 1e-83
Identities = 138/265 (52%), Positives = 178/265 (67%), Gaps = 11/265 (4%)
Frame = +2
Query 5 YSLFTYLAFFFLISNLCHSFNPRTFNVSQYGDPYGSASWSTTDATWYGDANGAGSTGGSC 184
+S L+ F L+ N C + + NVS+ WS ATWYG A GAGS GG+C
Sbjct 10 FSFIILLSLFSLLLNPCSCSHRKHLNVSKL---QSDTDWSPAGATWYGPAAGAGSDGGAC 66
Query 185 GYEDVVSSSPFSSTITALATSLYQDSKGCGTCYQVKCTGSPHCSGSPVTVVATDSCLGC- 361
GY D V +PFS+ ++A SL++ KGCG CYQVKCTG+ CSGSPVTV TD C GC
Sbjct 67 GYGDAVEKAPFSAKVSAGGPSLFKSGKGCGACYQVKCTGNEACSGSPVTVTITDECPGCV 126
Query 362 --DHLFDLSGTSFGKMANSGEADQLRNVGRLQIQYQKVDCEFSGVNVAFRVDTG-NPSYI 532
FDLSGT+FG MA G+ADQLRN G LQIQY++V+CE+ G +VAF VD+G NP+Y
Sbjct 127 SESTHFDLSGTAFGAMATPGQADQLRNAGILQIQYRRVECEYPGASVAFHVDSGSNPNYF 186
Query 533 AIAVEYEDGDG-LTEVAIRQANSDGDWLPMELSWGAVFRVNLPQGVTPPFSIKLT--DSS 703
A +EYEDGDG L V ++QA W+PM+ SWGAV++++ G+ PFSI+LT +S
Sbjct 187 ASLIEYEDGDGELAGVDLQQALHSNSWVPMQQSWGAVWKLDSGSGLRAPFSIRLTALESG 246
Query 704 KSVVAENVIPADYEI-TTYRSLVNF 775
++VA+NVIPA ++ TYRSLVNF
Sbjct 247 NTLVAKNVIPAGWQPGKTYRSLVNF 271
>ref|XP_002278559.1| PREDICTED: putative expansin-B2 [Vitis vinifera]
emb|CBI16588.3| unnamed protein product [Vitis vinifera]
Length=273
Score = 261 bits (668), Expect = 3e-82
Identities = 137/265 (52%), Positives = 177/265 (67%), Gaps = 11/265 (4%)
Frame = +2
Query 5 YSLFTYLAFFFLISNLCHSFNPRTFNVSQYGDPYGSASWSTTDATWYGDANGAGSTGGSC 184
+SL L+ F L+ N F+ + N S+ WS ATWYG ANGAGS GG+C
Sbjct 10 FSLLILLSLFSLLLNPSSCFHLKLLNFSKL---QSDTDWSPAGATWYGPANGAGSDGGAC 66
Query 185 GYEDVVSSSPFSSTITALATSLYQDSKGCGTCYQVKCTGSPHCSGSPVTVVATDSCLGC- 361
GYED V +PFS+ ++A SL++ KGCG CYQVKCTG+ CSGSPVTV TD C GC
Sbjct 67 GYEDAVEKAPFSAKVSAGGPSLFKSGKGCGACYQVKCTGNEACSGSPVTVTITDECPGCV 126
Query 362 --DHLFDLSGTSFGKMANSGEADQLRNVGRLQIQYQKVDCEFSGVNVAFRVDTG-NPSYI 532
FDLSGT+FG MA SG+ DQLRN G LQIQY++V+CE+ G +V F VD+G N +Y
Sbjct 127 SESTHFDLSGTAFGAMAKSGQDDQLRNAGVLQIQYRRVECEYPGTSVVFHVDSGSNSNYF 186
Query 533 AIAVEYEDGDG-LTEVAIRQANSDGDWLPMELSWGAVFRVNLPQGVTPPFSIKLT--DSS 703
A +EYEDGDG L V ++QA W+PM+ SWGAV++++ + PFSIKLT +S
Sbjct 187 ASLIEYEDGDGDLARVYLQQALDSDMWVPMQQSWGAVWKLDYGSELRAPFSIKLTALESG 246
Query 704 KSVVAENVIPADYEI-TTYRSLVNF 775
++VA+NVIPA ++ TYRS+VNF
Sbjct 247 NTLVAKNVIPAGWQPGKTYRSVVNF 271
>ref|NP_001234859.1| beta-expansin precursor [Solanum lycopersicum]
gb|ABB83474.1| beta-expansin precursor [Solanum lycopersicum]
Length=275
Score = 261 bits (667), Expect = 5e-82
Identities = 138/266 (52%), Positives = 176/266 (66%), Gaps = 12/266 (5%)
Frame = +2
Query 11 LFTYLAFFFLISNLCHSFNPRTFNVSQYGDPYGSASWSTTDATWYGDANGAGSTGGSCGY 190
+FTY F L++ C +P+ FNVS+ ++ WS ATWYG +GAGS GG+CGY
Sbjct 13 VFTYFTTFSLLTITCSCLHPKRFNVSKV---QSNSDWSPPGATWYGSPSGAGSDGGACGY 69
Query 191 EDVVSSSPFSSTITALATSLYQDSKGCGTCYQVKCTGSPHCSGSPVTVVATDSCLGCDHL 370
+ V PFSS I A SL++ KGCG CYQVKCTG+ CSG PV VV TDSC G L
Sbjct 70 TNAVDQPPFSSLIAAAGPSLFKSGKGCGACYQVKCTGNKACSGKPVRVVITDSCPGGPCL 129
Query 371 -----FDLSGTSFGKMANSGEADQLRNVGRLQIQYQKVDCEFSGVNVAFRVDTG-NPSYI 532
FDLSGTSFG MA SG+ADQLRN G +QIQY++V+C + GV + F VD+G N +Y
Sbjct 130 SESAHFDLSGTSFGAMAISGQADQLRNAGVIQIQYKRVECNYPGVKLTFHVDSGSNSNYF 189
Query 533 AIAVEYEDGDG-LTEVAIRQANSDGDWLPMELSWGAVFRVNLPQGVTPPFSIKLT-DSSK 706
A VEYEDGDG L V ++QA W PM+ SWGAV++++ + PFS+KLT +S +
Sbjct 190 AALVEYEDGDGDLNSVDLKQALDSESWQPMQQSWGAVWKLDSGSSLVAPFSLKLTGESGQ 249
Query 707 SVVAENVIPADYEI-TTYRSLVNFGV 781
+VVA VIPA ++ TYRS+VNF V
Sbjct 250 TVVANGVIPAGWQPGKTYRSVVNFKV 275
>gb|ABC47127.1| beta expansin 1 precursor [Solanum tuberosum]
Length=276
Score = 258 bits (660), Expect = 5e-81
Identities = 134/268 (50%), Positives = 175/268 (65%), Gaps = 11/268 (4%)
Frame = +2
Query 5 YSLFTYLAFFFLISNLCHSFNPRTFNVSQYGDPYGSASWSTTDATWYGDANGAGSTGGSC 184
+++FTY F +++ C F+P+ N ++ WS ATWYG +GAGS GG+C
Sbjct 11 FTIFTYFTLFSILTISCSCFHPKRLN--NVSKIQSNSDWSPAGATWYGSPSGAGSDGGAC 68
Query 185 GYEDVVSSSPFSSTITALATSLYQDSKGCGTCYQVKCTGSPHCSGSPVTVVATDSCLGCD 364
GY + V PFSS I A SL++ KGCG CYQVKCTG+ CSG PV VV TDSC G
Sbjct 69 GYTNAVDQPPFSSLIAAAGPSLFKSGKGCGACYQVKCTGNKACSGKPVRVVITDSCPGGP 128
Query 365 HL-----FDLSGTSFGKMANSGEADQLRNVGRLQIQYQKVDCEFSGVNVAFRVDTG-NPS 526
L FDLSGTSFG MA SG+ADQLRN G LQ+QY++V+C + GV + F VD+G N +
Sbjct 129 CLSESAHFDLSGTSFGAMAISGQADQLRNAGVLQVQYKRVECNYPGVKLTFHVDSGSNSN 188
Query 527 YIAIAVEYEDGDG-LTEVAIRQANSDGDWLPMELSWGAVFRVNLPQGVTPPFSIKLT-DS 700
Y A VEYEDGDG L V ++QA W PM+ SWGAV++++ + PFS+KLT +S
Sbjct 189 YFAALVEYEDGDGDLNRVDLKQALDSESWQPMQQSWGAVWKLDSGSSLVAPFSLKLTGES 248
Query 701 SKSVVAENVIPADYEI-TTYRSLVNFGV 781
++VVA VIPA ++ TYRS++NF V
Sbjct 249 GQTVVANGVIPAGWQPGKTYRSVINFKV 276
>ref|XP_002320708.1| hypothetical protein POPTRDRAFT_572072 [Populus trichocarpa]
gb|EEE99023.1| hypothetical protein POPTRDRAFT_572072 [Populus trichocarpa]
Length=274
Score = 251 bits (640), Expect = 5e-78
Identities = 138/263 (52%), Positives = 172/263 (65%), Gaps = 17/263 (6%)
Frame = +2
Query 23 LAFFFLISNLCHSFNPRTFNVSQYGDPYGSASWSTTDATWYGDANGAGSTGGSCGYEDVV 202
LAFF + N CH F+P+ FNVS+ ++ WS ATWYG GAGS GG+CGY + V
Sbjct 16 LAFFLV--NSCHCFHPKLFNVSKI---QSNSDWSPAGATWYGSPTGAGSDGGACGYGNAV 70
Query 203 SSSPFSSTITALATSLYQDSKGCGTCYQVKCTGSPH--CSGSPVTVVATDSCLGC----- 361
+PFSS I+A S+Y+ +GCG CYQVKCT S + CSG+PVTVV TD C G
Sbjct 71 EQAPFSSFISAGGDSIYKSGQGCGACYQVKCTSSSNAACSGNPVTVVITDQCPGSPCAQE 130
Query 362 DHLFDLSGTSFGKMANSGEADQLRNVGRLQIQYQKVDCEFSGVNVAFRVDTG-NPSYIAI 538
FDLSGT+FG MA SG+ DQLRN G LQIQ+Q+V C + G V F VD+G NP Y A
Sbjct 131 SFHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWPGKTVTFHVDSGSNPYYFAT 190
Query 539 AVEYEDGDG-LTEVAIRQANSDGDWLPMELSWGAVFRVNLPQGVTPPFSIKLT--DSSKS 709
VEYEDGDG L V ++QA W+PM+ SWGAV++++ + P SIKLT +S K+
Sbjct 191 VVEYEDGDGELKSVELKQALDSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKT 250
Query 710 VVAENVIPADYEI-TTYRSLVNF 775
+VA VIPA + TYRSLVNF
Sbjct 251 IVASGVIPAGWAPGQTYRSLVNF 273
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 570837566
Number of extensions: 13102436
Number of successful extensions: 42023
Number of sequences better than 1e-10: 40
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 41860
Number of HSP's successfully gapped: 41
Length of query: 1049
Length of database: 6150218869
Length adjustment: 141
Effective length of query: 908
Effective length of database: 3623628025
Effective search space: 753714629200
Effective search space used: 753714629200
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 177 (72.8 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDDHTJV013
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_7730
Length=549
Score E
Sequences producing significant alignments: (Bits) Value
gb|AAG23841.1|AF302808_1 metallothionein-like protein [Sesamu... 121 1e-32
gb|AAD02561.1| PGPS/NH21 [Petunia x hybrida] 107 7e-27
ref|XP_003631560.1| PREDICTED: EC protein homolog 1-like [Vit... 95.1 2e-22
gb|ABG57066.1| type 4 metallothionein [Arachis hypogaea] 91.7 5e-21
gb|ABG57067.1| type 4 metallothionein [Arachis hypogaea] 91.3 7e-21
ALIGNMENTS
>gb|AAG23841.1|AF302808_1 metallothionein-like protein [Sesamum indicum]
gb|AAR88997.1| seed-specific metallothionein-like protein [Sesamum indicum]
Length=83
Score = 121 bits (304), Expect = 1e-32
Identities = 46/63 (73%), Positives = 53/63 (84%), Gaps = 0/63 (0%)
Frame = -3
Query 547 VACDDRCGCPSPCPGDTTCRCASSGDDPTSMEHKQCPCGEHCGCNPCSCSKTEIKGTGKA 368
V CDDRCGCPSPCPG CRC++ G D T+ EHKQC CGEHCGCNPC+CSK+EI+GTGKA
Sbjct 9 VVCDDRCGCPSPCPGGIACRCSTGGGDDTTTEHKQCTCGEHCGCNPCTCSKSEIRGTGKA 68
Query 367 HCK 359
C+
Sbjct 69 FCR 71
>gb|AAD02561.1| PGPS/NH21 [Petunia x hybrida]
Length=88
Score = 107 bits (266), Expect = 7e-27
Identities = 44/66 (67%), Positives = 49/66 (74%), Gaps = 5/66 (8%)
Frame = -3
Query 541 CDDRCGCPSPCPGDTTCRCASSGDDPTS---MEHKQCPCGEHCGCNPCSCSKTE--IKGT 377
CD+RCGCPSPCPG CRCAS G MEHK+CPCGEHCGCNPC+C K+E G+
Sbjct 11 CDERCGCPSPCPGGVACRCASGGAATAGGGDMEHKKCPCGEHCGCNPCTCPKSEGTTAGS 70
Query 376 GKAHCK 359
GKAHCK
Sbjct 71 GKAHCK 76
>ref|XP_003631560.1| PREDICTED: EC protein homolog 1-like [Vitis vinifera]
Length=78
Score = 95.1 bits (235), Expect = 2e-22
Identities = 34/61 (56%), Positives = 45/61 (74%), Gaps = 2/61 (3%)
Frame = -3
Query 541 CDDRCGCPSPCPGDTTCRCASSGDDPTSMEHKQCPCGEHCGCNPCSCSKTEIKGTGKAHC 362
CD CGCP PCPG C+C+++G ++ +H CPCGEHCGCNPC+C + E+ GTGK +C
Sbjct 8 CDAGCGCPVPCPGGVDCKCSTTG--ASAADHSTCPCGEHCGCNPCTCPRGEVTGTGKLYC 65
Query 361 K 359
K
Sbjct 66 K 66
>gb|ABG57066.1| type 4 metallothionein [Arachis hypogaea]
Length=82
Score = 91.7 bits (226), Expect = 5e-21
Identities = 34/56 (61%), Positives = 39/56 (70%), Gaps = 0/56 (0%)
Frame = -3
Query 541 CDDRCGCPSPCPGDTTCRCASSGDDPTSMEHKQCPCGEHCGCNPCSCSKTEIKGTG 374
C+DRCGC PCPGD+TCRCAS + +H CPCGEHC CNPC+C KT G G
Sbjct 12 CNDRCGCSVPCPGDSTCRCASGNEGGGGTQHLTCPCGEHCECNPCTCPKTVAAGAG 67
>gb|ABG57067.1| type 4 metallothionein [Arachis hypogaea]
Length=82
Score = 91.3 bits (225), Expect = 7e-21
Identities = 34/56 (61%), Positives = 39/56 (70%), Gaps = 0/56 (0%)
Frame = -3
Query 541 CDDRCGCPSPCPGDTTCRCASSGDDPTSMEHKQCPCGEHCGCNPCSCSKTEIKGTG 374
C+DRCGC PCPGD+TCRCAS + +H CPCGEHC CNPC+C KT G G
Sbjct 12 CNDRCGCSIPCPGDSTCRCASGSEGGGETQHLTCPCGEHCECNPCTCPKTFAAGAG 67
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 5957142216
Number of extensions: 130875610
Number of successful extensions: 374141
Number of sequences better than 1e-10: 32
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 372275
Number of HSP's successfully gapped: 32
Length of query: 549
Length of database: 6150218869
Length adjustment: 132
Effective length of query: 417
Effective length of database: 3784899781
Effective search space: 193029888831
Effective search space used: 193029888831
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 172 (70.9 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDDKX9701N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_7820
Length=546
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002274108.2| PREDICTED: major allergen Pru ar 1 [Vitis... 195 3e-60
emb|CAN75490.1| hypothetical protein VITISV_017149 [Vitis vin... 193 2e-59
ref|XP_003631944.1| PREDICTED: LOW QUALITY PROTEIN: major all... 187 4e-57
ref|XP_002274309.1| PREDICTED: major allergen Pru ar 1 [Vitis... 184 5e-56
emb|CAC16166.1| pathogenesis-related protein 10 [Vitis vinifera] 182 3e-55
ALIGNMENTS
>ref|XP_002274108.2| PREDICTED: major allergen Pru ar 1 [Vitis vinifera]
Length=159
Score = 195 bits (495), Expect = 3e-60
Identities = 96/147 (65%), Positives = 117/147 (80%), Gaps = 0/147 (0%)
Frame = -2
Query 545 SSIPAAKLFKSGVLDLDTLMPKIMPQAIKSVEILEGDGGVGTVKIIHFGEGSPFKNVKHR 366
SSIP AK+FK+ VLD D L+PKI+PQAIK+VEI++GDGG GT+K I+FGEGS FK+V HR
Sbjct 12 SSIPPAKMFKASVLDADNLIPKILPQAIKNVEIIQGDGGPGTIKKIYFGEGSQFKSVTHR 71
Query 365 VEAIDMVNLTYTYSIIGGDALGCVIESITNHTKIVPTEDGGSVCKNTSTYRTKDGVELDE 186
V+ ID N TY+YSII GDAL ++ESI+ K+V + DGGS+CKN S Y TKD +DE
Sbjct 72 VDGIDKENFTYSYSIIEGDALMGILESISYEVKLVASPDGGSICKNISKYHTKDDAVIDE 131
Query 185 EKIKKGKDTAMMMFKAIEAYLLANPDA 105
E+IK GK+ A MFKAIEAYLLANPDA
Sbjct 132 EQIKAGKEKASGMFKAIEAYLLANPDA 158
>emb|CAN75490.1| hypothetical protein VITISV_017149 [Vitis vinifera]
Length=159
Score = 193 bits (490), Expect = 2e-59
Identities = 95/147 (65%), Positives = 115/147 (78%), Gaps = 0/147 (0%)
Frame = -2
Query 545 SSIPAAKLFKSGVLDLDTLMPKIMPQAIKSVEILEGDGGVGTVKIIHFGEGSPFKNVKHR 366
SSIP AK+FK+ +LD D L+PK PQAIKSVEI++G+GG GT+K IHFGEGS FK+V HR
Sbjct 12 SSIPPAKMFKAAILDSDNLIPKARPQAIKSVEIIQGEGGPGTIKKIHFGEGSQFKSVTHR 71
Query 365 VEAIDMVNLTYTYSIIGGDALGCVIESITNHTKIVPTEDGGSVCKNTSTYRTKDGVELDE 186
V+ ID N TY+YSII GDAL ++ESI+ K+V + DGGS+CKN S Y TKD +DE
Sbjct 72 VDGIDKENFTYSYSIIEGDALMGILESISYEVKLVASPDGGSICKNISKYHTKDDAVIDE 131
Query 185 EKIKKGKDTAMMMFKAIEAYLLANPDA 105
E+IK GK+ A MFKAIEAYLLANPDA
Sbjct 132 EQIKAGKEKASGMFKAIEAYLLANPDA 158
>ref|XP_003631944.1| PREDICTED: LOW QUALITY PROTEIN: major allergen Pru ar 1-like
[Vitis vinifera]
Length=159
Score = 187 bits (474), Expect = 4e-57
Identities = 91/147 (62%), Positives = 114/147 (78%), Gaps = 0/147 (0%)
Frame = -2
Query 545 SSIPAAKLFKSGVLDLDTLMPKIMPQAIKSVEILEGDGGVGTVKIIHFGEGSPFKNVKHR 366
SSIP AK+FK+ VLD+D L+PKI+PQ IK VEI+EGDGG G +K + FGEGS F VKHR
Sbjct 12 SSIPPAKMFKAFVLDVDKLIPKILPQTIKCVEIIEGDGGAGIIKKVTFGEGSQFNYVKHR 71
Query 365 VEAIDMVNLTYTYSIIGGDALGCVIESITNHTKIVPTEDGGSVCKNTSTYRTKDGVELDE 186
V+ ID N TYTYS+I GDAL +ESI+ K+VP+ DGGS+CK+TS Y TK E+ E
Sbjct 72 VDGIDKENFTYTYSVIEGDALMGTLESISYEVKLVPSPDGGSICKSTSKYHTKGDFEITE 131
Query 185 EKIKKGKDTAMMMFKAIEAYLLANPDA 105
++IK GK+ AM +FKA+EAYLLA+PDA
Sbjct 132 DQIKAGKEKAMGLFKAVEAYLLAHPDA 158
>ref|XP_002274309.1| PREDICTED: major allergen Pru ar 1 [Vitis vinifera]
emb|CAN75908.1| hypothetical protein VITISV_005514 [Vitis vinifera]
emb|CBI22935.3| unnamed protein product [Vitis vinifera]
Length=159
Score = 184 bits (467), Expect = 5e-56
Identities = 88/147 (60%), Positives = 116/147 (79%), Gaps = 0/147 (0%)
Frame = -2
Query 545 SSIPAAKLFKSGVLDLDTLMPKIMPQAIKSVEILEGDGGVGTVKIIHFGEGSPFKNVKHR 366
SSIP AK+FK+ +LD D L+PK+ PQAIKSVEI++G+GG GT+K IHFGEGS FK++ HR
Sbjct 12 SSIPPAKMFKAAILDSDNLIPKVRPQAIKSVEIIQGEGGPGTIKKIHFGEGSKFKSMTHR 71
Query 365 VEAIDMVNLTYTYSIIGGDALGCVIESITNHTKIVPTEDGGSVCKNTSTYRTKDGVELDE 186
V+AID N T++Y+++ GD L IESI++ K+V + DGGS+ KNTS Y TK VE+ E
Sbjct 72 VDAIDKENFTFSYTVVDGDVLTGGIESISHELKVVASPDGGSIYKNTSKYHTKGDVEISE 131
Query 185 EKIKKGKDTAMMMFKAIEAYLLANPDA 105
E IK GK+ A+ +FKAIEAY+LA+PDA
Sbjct 132 EHIKGGKEEALALFKAIEAYVLAHPDA 158
>emb|CAC16166.1| pathogenesis-related protein 10 [Vitis vinifera]
Length=159
Score = 182 bits (462), Expect = 3e-55
Identities = 86/147 (59%), Positives = 116/147 (79%), Gaps = 0/147 (0%)
Frame = -2
Query 545 SSIPAAKLFKSGVLDLDTLMPKIMPQAIKSVEILEGDGGVGTVKIIHFGEGSPFKNVKHR 366
SS+P AK+FK+ +LD D L+PK+ PQAIKSVEI++G+GG GT+K IHFGEGS FK++ HR
Sbjct 12 SSVPPAKMFKAAILDSDNLIPKVRPQAIKSVEIIQGEGGPGTIKKIHFGEGSKFKSMTHR 71
Query 365 VEAIDMVNLTYTYSIIGGDALGCVIESITNHTKIVPTEDGGSVCKNTSTYRTKDGVELDE 186
V+AID N T++Y+++ GD L IESI++ K+V + DGGS+ KNTS Y TK VE+ E
Sbjct 72 VDAIDKENFTFSYTVVDGDVLTGGIESISHELKVVASPDGGSIYKNTSKYHTKGDVEICE 131
Query 185 EKIKKGKDTAMMMFKAIEAYLLANPDA 105
E +K GK+ A+ +FKAIEAY+LA+PDA
Sbjct 132 EHVKGGKEEALALFKAIEAYVLAHPDA 158
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 1,855,251,573
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 250071044
Number of extensions: 4885508
Number of successful extensions: 15032
Number of sequences better than 1e-10: 167
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 14924
Number of HSP's successfully gapped: 167
Length of query: 546
Length of database: 6150218869
Length adjustment: 132
Effective length of query: 414
Effective length of database: 3784899781
Effective search space: 189244989050
Effective search space used: 189244989050
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 172 (70.9 bits)
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDDSH6H01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_8348
Length=528
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_003635319.1| PREDICTED: kidney mitochondrial carrier p... 150 3e-43
dbj|BAE71294.1| putative mitochondrial dicarboxylate carrier ... 152 7e-42
gb|ABK95510.1| unknown [Populus trichocarpa] 148 9e-42
ref|XP_003613078.1| Mitochondrial 2-oxoglutarate/malate carri... 152 1e-41
ref|XP_002285693.1| PREDICTED: probable mitochondrial 2-oxogl... 152 1e-41
ALIGNMENTS
>ref|XP_003635319.1| PREDICTED: kidney mitochondrial carrier protein 1-like [Vitis
vinifera]
Length=151
Score = 150 bits (380), Expect = 3e-43
Identities = 73/81 (90%), Positives = 78/81 (96%), Gaps = 0/81 (0%)
Frame = -2
Query 527 AAGFVASVASNPVDVIKTRVMNMKVEAGVAPPYAGAVDCAMKTIRAEGPMALYKGFIPTI 348
AAGFVA+VASNPVDVIKTRVMNMKVE G APPY GA+DCA+KT+RAEGPMALYKGFIPTI
Sbjct 68 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAPPYTGALDCALKTVRAEGPMALYKGFIPTI 127
Query 347 SRQGPFTVVLFVTLEQVRKLL 285
SRQGPFT+VLFVTLEQVRKLL
Sbjct 128 SRQGPFTIVLFVTLEQVRKLL 148
>dbj|BAE71294.1| putative mitochondrial dicarboxylate carrier protein [Trifolium
pratense]
Length=324
Score = 152 bits (384), Expect = 7e-42
Identities = 76/81 (94%), Positives = 79/81 (98%), Gaps = 0/81 (0%)
Frame = -2
Query 527 AAGFVASVASNPVDVIKTRVMNMKVEAGVAPPYAGAVDCAMKTIRAEGPMALYKGFIPTI 348
AAGFVA+VASNPVDVIKTRVMNMKVEAG PPYAGA+DCAMKTIRAEGPMALYKGFIPTI
Sbjct 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGKEPPYAGALDCAMKTIRAEGPMALYKGFIPTI 300
Query 347 SRQGPFTVVLFVTLEQVRKLL 285
SRQGPFTVVLFVTLEQVRK+L
Sbjct 301 SRQGPFTVVLFVTLEQVRKVL 321
>gb|ABK95510.1| unknown [Populus trichocarpa]
Length=195
Score = 148 bits (374), Expect = 9e-42
Identities = 72/80 (90%), Positives = 77/80 (96%), Gaps = 0/80 (0%)
Frame = -2
Query 524 AGFVASVASNPVDVIKTRVMNMKVEAGVAPPYAGAVDCAMKTIRAEGPMALYKGFIPTIS 345
AGFVASVASNP+DVIKTRVMNMKVE GV PPY GA+DCA+KT++AEGPMALYKGFIPTIS
Sbjct 113 AGFVASVASNPIDVIKTRVMNMKVEPGVEPPYKGALDCALKTVKAEGPMALYKGFIPTIS 172
Query 344 RQGPFTVVLFVTLEQVRKLL 285
RQGPFTVVLFVTLEQVRKLL
Sbjct 173 RQGPFTVVLFVTLEQVRKLL 192
>ref|XP_003613078.1| Mitochondrial 2-oxoglutarate/malate carrier protein [Medicago
truncatula]
gb|AES96036.1| Mitochondrial 2-oxoglutarate/malate carrier protein [Medicago
truncatula]
Length=322
Score = 152 bits (383), Expect = 1e-41
Identities = 75/81 (93%), Positives = 79/81 (98%), Gaps = 0/81 (0%)
Frame = -2
Query 527 AAGFVASVASNPVDVIKTRVMNMKVEAGVAPPYAGAVDCAMKTIRAEGPMALYKGFIPTI 348
AAGFVA+VASNPVDVIKTRVMNMKVEAG PPYAGA+DCA+KT+RAEGPMALYKGFIPTI
Sbjct 239 AAGFVAAVASNPVDVIKTRVMNMKVEAGKEPPYAGALDCALKTVRAEGPMALYKGFIPTI 298
Query 347 SRQGPFTVVLFVTLEQVRKLL 285
SRQGPFTVVLFVTLEQVRKLL
Sbjct 299 SRQGPFTVVLFVTLEQVRKLL 319
>ref|XP_002285693.1| PREDICTED: probable mitochondrial 2-oxoglutarate/malate carrier
protein-like [Vitis vinifera]
Length=323
Score = 152 bits (383), Expect = 1e-41
Identities = 74/81 (91%), Positives = 79/81 (98%), Gaps = 0/81 (0%)
Frame = -2
Query 527 AAGFVASVASNPVDVIKTRVMNMKVEAGVAPPYAGAVDCAMKTIRAEGPMALYKGFIPTI 348
AAGFVA+VASNPVDVIKTRVMNMKVE G APPY+GA+DCAMKT+RAEGPMALYKGFIPTI
Sbjct 240 AAGFVAAVASNPVDVIKTRVMNMKVEPGTAPPYSGALDCAMKTVRAEGPMALYKGFIPTI 299
Query 347 SRQGPFTVVLFVTLEQVRKLL 285
SRQGPFTVVLFVTLEQVRK+L
Sbjct 300 SRQGPFTVVLFVTLEQVRKIL 320
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 225233770
Number of extensions: 4142770
Number of successful extensions: 11112
Number of sequences better than 1e-10: 54
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 10828
Number of HSP's successfully gapped: 54
Length of query: 528
Length of database: 6150218869
Length adjustment: 131
Effective length of query: 397
Effective length of database: 3802818865
Effective search space: 171126848925
Effective search space used: 171126848925
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 172 (70.9 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDEWA8B01N
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_9225
Length=503
Score E
Sequences producing significant alignments: (Bits) Value
ref|XP_002305027.1| predicted protein [Populus trichocarpa] >... 167 3e-46
ref|XP_002331906.1| predicted protein [Populus trichocarpa] >... 166 1e-45
ref|NP_186784.1| putative peptide/nitrate transporter [Arabid... 165 3e-45
ref|XP_003633812.1| PREDICTED: probable peptide/nitrate trans... 159 5e-43
emb|CBI34989.3| unnamed protein product [Vitis vinifera] 159 5e-43
ALIGNMENTS
>ref|XP_002305027.1| predicted protein [Populus trichocarpa]
gb|EEE85538.1| predicted protein [Populus trichocarpa]
Length=540
Score = 167 bits (424), Expect = 3e-46
Identities = 93/141 (66%), Positives = 114/141 (81%), Gaps = 6/141 (4%)
Frame = +3
Query 3 AAITEEKRLEVSQGVDFSTE----DVKFSIFWLLPQYVLLGVSDIFTVVGMQEYFYSGVP 170
AA+ E KRLE+ Q ++ + +V SIFWLLPQY+LLG+SDIFTVVGMQE+FYS VP
Sbjct 393 AALVETKRLEIGQKMEVVDQKMETEVPLSIFWLLPQYILLGISDIFTVVGMQEFFYSEVP 452
Query 171 VRMRTMGIALNTSVFGVGSFLSAILISVIENLTSGEG--NWFDDDMSKARLDKYYWLLAI 344
VRMRTMGIAL TSVFGVGSFLSA+LIS++E TS G +WF DDM +ARLDKYYWLLA+
Sbjct 453 VRMRTMGIALYTSVFGVGSFLSALLISLVEYFTSSTGGKSWFSDDMREARLDKYYWLLAL 512
Query 345 ssvvsllgfvvfCKCFSYRTT 407
SV+SL+ +VVFCKC+ R++
Sbjct 513 LSVLSLIFYVVFCKCYVSRSS 533
>ref|XP_002331906.1| predicted protein [Populus trichocarpa]
gb|EEF11709.1| predicted protein [Populus trichocarpa]
Length=540
Score = 166 bits (420), Expect = 1e-45
Identities = 92/142 (65%), Positives = 113/142 (80%), Gaps = 7/142 (5%)
Frame = +3
Query 3 AAITEEKRLEVSQGVDFSTE----DVKFSIFWLLPQYVLLGVSDIFTVVGMQEYFYSGVP 170
AA+ E KRLE+S+ ++ +V SIFWLLPQY+LLG+SDIFTVVGMQE+FYS VP
Sbjct 393 AALVETKRLEISRKMEVLDPKLETEVPLSIFWLLPQYILLGISDIFTVVGMQEFFYSEVP 452
Query 171 VRMRTMGIALNTSVFGVGSFLSAILISVIENLTSGEG---NWFDDDMSKARLDKYYWLLA 341
VRMRTMGIAL TSVFGVGSFLSA+LIS++E TS G +WF DDM +AR DKYYWLLA
Sbjct 453 VRMRTMGIALYTSVFGVGSFLSALLISLVEYFTSSRGKGRSWFSDDMREARFDKYYWLLA 512
Query 342 IssvvsllgfvvfCKCFSYRTT 407
+ SV+SL+ +V+FCKCF R++
Sbjct 513 LLSVLSLVFYVIFCKCFVSRSS 534
>ref|NP_186784.1| putative peptide/nitrate transporter [Arabidopsis thaliana]
sp|Q9SRI2.1|PTR31_ARATH RecName: Full=Putative peptide/nitrate transporter At3g01350
gb|AAF03501.1|AC010676_11 putative peptide transporter [Arabidopsis thaliana]
gb|AEE73654.1| putative peptide/nitrate transporter [Arabidopsis thaliana]
Length=563
Score = 165 bits (418), Expect = 3e-45
Identities = 89/137 (65%), Positives = 106/137 (77%), Gaps = 7/137 (5%)
Frame = +3
Query 3 AAITEEKRLEVSQGV----DFSTEDVKFSIFWLLPQYVLLGVSDIFTVVGMQEYFYSGVP 170
AAI E KRL +SQ + D+ E V SIFWLLPQY+LLG+SDIFTVVGMQE+FYS VP
Sbjct 412 AAIVERKRLAISQKMKTLPDYDPETVPLSIFWLLPQYILLGISDIFTVVGMQEFFYSEVP 471
Query 171 VRMRTMGIALNTSVFGVGSFLSAILISVIENLTSGEG---NWFDDDMSKARLDKYYWLLA 341
VRMRTMG AL TSVFGVGSF+SA LIS++E +S G NWF DDMS+ARLDKYYWLLA
Sbjct 472 VRMRTMGFALYTSVFGVGSFVSAALISIVEAYSSSTGDRQNWFADDMSEARLDKYYWLLA 531
Query 342 IssvvsllgfvvfCKCF 392
++S +S + ++ CK F
Sbjct 532 LTSTISFVVYIFLCKFF 548
>ref|XP_003633812.1| PREDICTED: probable peptide/nitrate transporter At5g14940-like
[Vitis vinifera]
Length=533
Score = 159 bits (401), Expect = 5e-43
Identities = 84/136 (62%), Positives = 106/136 (78%), Gaps = 2/136 (1%)
Frame = +3
Query 3 AAITEEKRLEVSQGVDFSTEDVKFSIFWLLPQYVLLGVSDIFTVVGMQEYFYSGVPVRMR 182
AA+ E+KRL +S ++ +E V SIFWLLPQY++LG++DIFTVVGMQE+FY VP+RMR
Sbjct 392 AALVEKKRLSISSKLESQSETVPLSIFWLLPQYIILGIADIFTVVGMQEFFYGEVPIRMR 451
Query 183 TMGIALNTSVFGVGSFLSAILISVIENLTS--GEGNWFDDDMSKARLDKYYWLLAIssvv 356
TMGIAL +SVFGVGSF+SA LI +IE TS G WF DDM +ARLDKYYWLLA+SS
Sbjct 452 TMGIALYSSVFGVGSFMSAFLIWLIEIFTSSGGRQGWFSDDMREARLDKYYWLLALSSTF 511
Query 357 sllgfvvfCKCFSYRT 404
SLL +++ CK + R+
Sbjct 512 SLLLYMLLCKYYKSRS 527
>emb|CBI34989.3| unnamed protein product [Vitis vinifera]
Length=549
Score = 159 bits (401), Expect = 5e-43
Identities = 84/136 (62%), Positives = 106/136 (78%), Gaps = 2/136 (1%)
Frame = +3
Query 3 AAITEEKRLEVSQGVDFSTEDVKFSIFWLLPQYVLLGVSDIFTVVGMQEYFYSGVPVRMR 182
AA+ E+KRL +S ++ +E V SIFWLLPQY++LG++DIFTVVGMQE+FY VP+RMR
Sbjct 392 AALVEKKRLSISSKLESQSETVPLSIFWLLPQYIILGIADIFTVVGMQEFFYGEVPIRMR 451
Query 183 TMGIALNTSVFGVGSFLSAILISVIENLTS--GEGNWFDDDMSKARLDKYYWLLAIssvv 356
TMGIAL +SVFGVGSF+SA LI +IE TS G WF DDM +ARLDKYYWLLA+SS
Sbjct 452 TMGIALYSSVFGVGSFMSAFLIWLIEIFTSSGGRQGWFSDDMREARLDKYYWLLALSSTF 511
Query 357 sllgfvvfCKCFSYRT 404
SLL +++ CK + R+
Sbjct 512 SLLLYMLLCKYYKSRS 527
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 228184033
Number of extensions: 4553992
Number of successful extensions: 13217
Number of sequences better than 1e-10: 12
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 13198
Number of HSP's successfully gapped: 12
Length of query: 503
Length of database: 6150218869
Length adjustment: 128
Effective length of query: 375
Effective length of database: 3856576117
Effective search space: 150406468563
Effective search space used: 150406468563
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDF0XVV01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_9521
Length=495
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 180021330
Number of extensions: 2986142
Number of successful extensions: 5972
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 5972
Number of HSP's successfully gapped: 0
Length of query: 495
Length of database: 6150218869
Length adjustment: 126
Effective length of query: 369
Effective length of database: 3892414285
Effective search space: 151804157115
Effective search space used: 151804157115
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362
A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and
David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new
generation of protein database search programs", Nucleic
Acids Res. 25:3389-3402.
RID: UPDG7UEW01S
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
17,919,084 sequences; 6,150,218,869 total letters
Query= TrVeIntZeamaGB1_9854
Length=485
No significant similarity found. For reasons why, click here.
Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF
excluding environmental samples from WGS projects
Posted date: Apr 23, 2012 4:44 PM
Number of letters in database: 6,150,218,869
Number of sequences in database: 17,919,084
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 17919084
Number of Hits to DB: 212431106
Number of extensions: 4031151
Number of successful extensions: 9662
Number of sequences better than 1e-10: 0
Number of HSP's better than 1e-10 without gapping: 0
Number of HSP's gapped: 9658
Number of HSP's successfully gapped: 0
Length of query: 485
Length of database: 6150218869
Length adjustment: 122
Effective length of query: 363
Effective length of database: 3964090621
Effective search space: 154599534219
Effective search space used: 154599534219
T: 12
A: 40
X1: 16 (7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 171 (70.5 bits)
ka-blk-alpha gapped: 1.9
ka-blk-alpha ungapped: 0.7916
ka-blk-alpha_v gapped: 42.6028
ka-blk-alpha_v ungapped: 4.96466
ka-blk-sigma gapped: 43.6362