Browsing by Author "Alford, Shannon R."

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    myo-Inositol oxygenase is required for responses to low energy conditions in Arabidopsis thaliana
    Alford, Shannon R.; Rangarajan, Padma; Williams, Phoebe; Gillaspy, Glenda E. (Frontiers, 2012-01-01)
    myo-Inositol is a precursor for cell wall components, is used as a backbone of myo-inositol trisphosphate (Ins(1,4,5)P3) and phosphateidylinositol phosphate signaling molecules, and is debated about whether it is also a precursor in an alternate ascorbic acid synthesis pathway. Plants control inositol homeostasis by regulation of key enzymes involved in myo-inositol synthesis and catabolism. Recent transcriptional profiling data indicate up-regulation of the myo-inositol oxygenase (MIOX) genes under conditions in which energy or nutrients are limited. To test whether the MIOX genes are required for responses to low energy, we first examined MIOX2 and MIOX4 gene expression regulation by energy/nutrient conditions. We found that both MIOX2 and MIOX4 expression are suppressed by exogenous glucose addition in the shoot, but not in the root. Both genes were abundantly expressed during low energy/nutrient conditions. Loss-of-function mutants in MIOX genes contain alterations in myo-inositol levels and growth changes in the root. Miox2 mutants can be complemented with a MIOX2:green fluorescent protein fusion. Further we show here that MIOX2 is a cytoplasmic protein, while MIOX4 is present mostly in the cytoplasm, but also occasionally in the nucleus. Together, these data suggest that MIOX catabolism in the shoot may influence root growth responses during low energy/nutrient conditions.
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    Parasitic Plants Striga and Phelipanche Dependent upon Exogenous Strigolactones for Germination Have Retained Genes for Strigolactone Biosynthesis
    Das, Malay; Fernández-Aparicio, Mónica; Yang, Zhenzhen; Huang, Kan; Wickett, Norman J.; Alford, Shannon R.; Wafula, Eric K.; dePamphilis, Claude W.; Bouwmeester, Harro; Timko, Michael P.; Yoder, John I.; Westwood, James H. (2015-05)
    Strigolactones are plant hormones with multiple functions, including regulating various aspects of plant architecture such as shoot branching, facilitating the colonization of plant roots by arbuscular mycorrhizal fungi, and acting as seed germination stimulants for certain parasitic plants of the family Orobanchaceae. The obligate parasitic species Phelipanche aegyptiaca and Striga hermonthica require strigolactones for germination, while the facultative parasite Triphysaria versicolor does not. It has been hypothesized that P. aegyptiaca and S. hermonthica would have undergone evolutionary loss of strigolactone biosynthesis as a part of their mechanism to enable specific detection of exogenous strigolactones. We analyzed the transcriptomes of P. aegyptiaca, S. hermonthica and T. versicolor and identified genes known to act in strigolactone synthesis (D27, CCD7, CCD8, and MAX1), perception (MAX2 and D14) and transport (PDR12). These genes were then analyzed to assess likelihood of function. Transcripts of all strigolactone-related genes were found in P. aegyptiaca and S. hermonthica, and evidence points to their encoding functional proteins. Gene open reading frames were consistent with homologs from Arabidopsis and other strigolactone-producing plants, and all genes were expressed in parasite tissues. In general, the genes related to strigolactone synthesis and perception appeared to be evolving under codon-based selective constraints in strigolactone-dependent species. Bioassays of S. hermonthica root extracts indicated the presence of strigolactone class stimulants on germination of P. aegyptiaca seeds. Taken together, these results indicate that Phelipanche aegyptiaca and S. hermonthica have retained functional genes involved in strigolactone biosynthesis, suggesting that the parasites use both endogenous and exogenous strigolactones and have mechanisms to differentiate the two.
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    Variation in soil-test-based phosphorus and potassium rate recommendations across the southern USA
    Zhang, Hailin; Antonangelo, Joao; Grove, John; Osmond, Deanna; Slaton, Nathan A.; Alford, Shannon R.; Florence, Robert; Huluka, Gobena; Hardy, David Herring; Lessl, Jason; Maguire, Rory O.; Mylavarapu, Rao; Oldham, J. Larry; Pena-Yewtukhiw, Eugenia M.; Provin, Tony; Sonon, Leticia; Sotomayor, David; Wang, Jim (2021-05-18)
    Thirteen states associated with the Southern Extension and Research Activities Information Exchange Group-6 (SERA-IEG-6) agreed to share their soil test based P and K rate recommendations for nine major crops. The objectives were to compare fertilizer P and K rate recommendations, to look for opportunities to rationalize similar recommendations across state lines, and to examine challenges to the development of a cooperative regional approach to P and K recommendations. Mehlich-3 (eight states), Mehlich-1 (five states), or Lancaster (one state) extractions were the basis of plant available soil P (STP) and K (STK) assessment. Fertilizer recommendation philosophies (sufficiency, build and maintain, and/or hybrid) variation among the states might be the main reason behind such discrepancies. Although a few similarities in P and K rate recommendations were found, the different philosophies, numerical presentations, and extraction procedures drove important recommendation differences. Widespread adoption of the Mehlich-3 extraction procedure has not reduced variation in fertilizer P and K rate recommendations among the states. Instead, for states using Mehlich 3, soil test critical concentrations ranged from 30 to 75 mg P kg(-1) and 60 to 175 mg K kg(-1) for corn (Zea mays L.) grain and warm-season grass hay production. The adoption of uniform soil testing terminology, sample collection guidelines, extraction methods, and interpretations across common physiographic regions, soils, and state lines remains a challenge. Differences arise because of the different soil orders and properties, climate conditions, and resulting crop responses to added P and K fertilizers. Such differences in soil-test-based fertilizer P and K recommendations are state specific and highlight needs to examine the soil testing and recommendation process, make soil test results end-user friendly, and, when appropriate, standardize fundamental information used in the soil testing guidelines.