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dc.contributor.authorIqbal, Zahiden
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorHussain, Hafiz Iftikharen
dc.contributor.authorHuang, Linglien
dc.contributor.authorHao, Haihongen
dc.contributor.authorYuan, Zonghuien
dc.date.accessioned2020-09-21T16:16:50Zen
dc.date.available2020-09-21T16:16:50Zen
dc.date.issued2016-10-14en
dc.identifierARTN 35442 (Article number)en
dc.identifier.issn2045-2322en
dc.identifier.othersrep35442 (PII)en
dc.identifier.urihttp://hdl.handle.net/10919/100040en
dc.description.abstractSeveral studies have been conducted to check the prevalence of methicillin-resistant strains of Staphylococcus aureus (MRSA) in animals and animal-derived food products but limited data are available regarding their virulence and associated gene expression profile. In the present study, antibiotic resistance and virulence of MRSA and methicillin-sensitive S. aureus animal isolates were determined in vitro by agar dilution, biofilm formation, adhesion, invasion and intracellular survivability assays. In addition, the pathogenicity of these isolates was examined in a murine model of S. aureus sepsis. MRSA1679a, a strain isolated from chicken, was observed to be highly virulent, in cell culture and in mouse model, and exhibited extensive resistant profile. Comparative gene expression profile of MRSA1679a and the reference human MRSA strain (ATCC 29213) was performed using Illumina-based transcriptome and RT-qPCR analyses. Several virulence elements including 22 toxin genes were detected in MRSA animal-isolate. In addition, we observed enhanced expression of crucial virulence regulators, such as sarA and KdpDE in MRSA animal-isolate compared to the human isolate. Collectively, gene expression profile including several virulence and drug-resistance factors confirmed the unique and highly virulent determinants of the MRSA strain of poultry origin which warrants further attention due to significant threat to public health.en
dc.format.extent12 page(s)en
dc.format.mediumElectronicen
dc.languageEnglishen
dc.publisherNature Publishing Groupen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSIGNAL-TRANSDUCTIONen
dc.subjectGENE-EXPRESSIONen
dc.subjectRESISTANCEen
dc.subjectSARAen
dc.subjectINFECTIONen
dc.subjectMRSAen
dc.subjectIDENTIFICATIONen
dc.subjectREGULATORen
dc.subjectPROTEINen
dc.subjectMURINEen
dc.subject.meshCell Lineen
dc.subject.meshAnimalsen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshChickensen
dc.subject.meshSwineen
dc.subject.meshMiceen
dc.subject.meshStaphylococcal Infectionsen
dc.subject.meshPoultry Diseasesen
dc.subject.meshSwine Diseasesen
dc.subject.meshProtein Kinasesen
dc.subject.meshBacterial Proteinsen
dc.subject.meshTrans-Activatorsen
dc.subject.meshVirulenceen
dc.subject.meshFemaleen
dc.subject.meshMethicillin-Resistant Staphylococcus aureusen
dc.subject.meshTranscriptomeen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Proteinsen
dc.subject.meshCell Lineen
dc.subject.meshChickensen
dc.subject.meshFemaleen
dc.subject.meshMethicillin-Resistant Staphylococcus aureusen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshPoultry Diseasesen
dc.subject.meshProtein Kinasesen
dc.subject.meshStaphylococcal Infectionsen
dc.subject.meshSwineen
dc.subject.meshSwine Diseasesen
dc.subject.meshTrans-Activatorsen
dc.subject.meshTranscriptomeen
dc.subject.meshVirulenceen
dc.titleComparative virulence studies and transcriptome analysis of Staphylococcus aureus strains isolated from animalsen
dc.typeArticle - Refereeden
dc.date.updated2020-09-21T16:16:48Zen
dc.description.versionPublished (Publication status)en
dc.title.serialScientific Reportsen
dc.identifier.doihttps://doi.org/10.1038/srep35442en
dc.type.otherArticleen
dc.type.otherJournalen
dc.identifier.volume6en
dc.identifier.issue1en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458 (orcid)]en
dc.identifier.pmid27739497 (pubmed)en
dcterms.dateAccepted2016-09-29en
dc.identifier.eissn2045-2322en
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Techen


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Creative Commons Attribution 4.0 International
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