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dc.contributor.authorTraore, Sy Men_US
dc.contributor.authorZhao, Bingyuen_US
dc.date.accessioned2012-08-24T10:49:28Z
dc.date.available2012-08-24T10:49:28Z
dc.date.issued2011-12-07
dc.identifier.citationPlant Methods. 2011 Dec 07;7(1):42en_US
dc.identifier.urihttp://hdl.handle.net/10919/18779
dc.description.abstractBackground Cloning genes into plasmid vectors is one of the key steps for studying gene function. Recently, Invitrogen& developed a convenient Gateway® cloning system based on the site-specific DNA recombination properties of bacteriophage lambda and the cytotoxic protein ccdB, which is lethal to most E. coli strains. The ccdB protein, however, is not toxic to Agrobacterium tumefaciens, an important player often used for studying gene function in planta. This limits the direct application of the Gateway® cloning system in plant transformation-mediated research. Results In this study, we constructed a novel Gateway®-compatible destination vector, pEG101-SacB/R, by replacing the ccdB gene with a SacB-SacR gene cassette as the negative selectable marker. Conclusion Our results demonstrate that the new pEG101-SacB/R destination vector can be used for Gateway® cloning in Agrobacterium tumefaciens. pEG101-SacB/R will be a valuable tool for high-throughput functional analysis of genes in planta.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.rightsCreative Commons Attribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA novel Gateway(R)-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciensen_US
dc.typeArticle - Refereed
dc.date.updated2012-08-24T10:49:28Z
dc.description.versionPeer Reviewed
dc.rights.holderSy Traore et al.; licensee BioMed Central Ltd.en_US
dc.title.serialPlant Methods
dc.identifier.doihttps://doi.org/10.1186/1746-4811-7-42
dc.type.dcmitypeText


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International