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dc.contributor.authorYoungstrom, Daniel W.en
dc.contributor.authorBarrett, Jennifer G.en
dc.contributor.authorJose, Rod R.en
dc.contributor.authorKaplan, David L.en
dc.date.accessioned2013-06-05T20:36:22Zen
dc.date.available2013-06-05T20:36:22Zen
dc.date.issued2013-05-17en
dc.identifier.citationYoungstrom DW, Barrett JG, Jose RR, Kaplan DL (2013) Functional Characterization of Detergent-Decellularized Equine Tendon Extracellular Matrix for Tissue Engineering Applications. PLoS ONE 8(5): e64151. doi:10.1371/journal.pone.0064151en
dc.identifier.urihttp://hdl.handle.net/10919/23148en
dc.description.abstractNatural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.en
dc.description.sponsorshipThe authors would like to acknowledge partial funding by the Virginia Tech Institute for Critical Technology and Applied Science (ICTAS), the Morris Animal Foundation, and the Tissue Engineering Resource Center (TERC) at Tufts University [NIH P41 EB002520]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 3.0 United Statesen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en
dc.subjecttendonen
dc.subjecttissue engineeringen
dc.subjectequineen
dc.subjectextracellular matrixen
dc.titleFunctional Characterization of Detergent-Decellularized Equine Tendon Extracellular Matrix for Tissue Engineering Applicationsen
dc.typeArticle - Refereeden
dc.title.serialPLOS ONEen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0064151en
dc.identifier.volume8en
dc.identifier.issue5en
dc.type.dcmitypeTexten


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Creative Commons Attribution 3.0 United States
License: Creative Commons Attribution 3.0 United States