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dc.contributorVirginia Techen
dc.contributor.authorGilmore, Brian L.en
dc.contributor.authorTanner, Justin R.en
dc.contributor.authorMcKell, Allison O.en
dc.contributor.authorBoudreaux, Crystal E.en
dc.contributor.authorDukes, Madeline J.en
dc.contributor.authorMcDonald, Sarah M.en
dc.contributor.authorKelly, Deborah F.en
dc.date.accessioned2013-06-24T19:41:28Zen
dc.date.available2013-06-24T19:41:28Zen
dc.date.issued2013-03en
dc.identifier.issn2072-666Xen
dc.identifier.urihttp://hdl.handle.net/10919/23260en
dc.description13 p.en
dc.description.abstractHere we present new applications for silicon nitride (SiN) membranes to evaluate biological processes. We determined that 50-nanometer thin films of SiN produced from silicon wafers were sufficiently durable to bind active rotavirus assemblies. A direct comparison of SiN microchips with conventional carbon support films indicated that SiN performs equivalent to the traditional substrate to prepare samples for Electron Microscopy (EM) imaging. Likewise, SiN films coated with Ni-NTA affinity layers concentrated rotavirus particles similarly to affinity-coated carbon films. However, affinity-coated SiN membranes outperformed glow-discharged conventional carbon films 5-fold as indicated by the number of viral particles quantified in EM images. In addition, we were able to recapitulate viral uncoating and transcription mechanisms directed onto the microchip surfaces. EM images of these processes revealed the production of RNA transcripts emerging from active rotavirus complexes. These results were confirmed by the functional incorporation of radiolabeled nucleotides into the nascent RNA transcripts. Collectively, we demonstrate new uses for SiN membranes to perform molecular surveillance on life processes in real-time.en
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsilicon nitrideen
dc.subjectmicrochipsen
dc.subjectrotavirusen
dc.subjectRNAen
dc.subjectelectron microscopyen
dc.titleMolecular Surveillance of Viral Processes Using Silicon Nitride Membranesen
dc.typeArticle - Refereeden
dc.description.versionPublished versionen
dc.title.serialMicromachinesen
dc.identifier.doihttps://doi.org/10.3390/mi4010090en
dc.type.dcmitypeTexten
dc.identifier.sourceurlhttp://www.mdpi.com/2072-666X/4/1/90en


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International