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dc.contributorVirginia Tech
dc.contributor.authorGilmore, Brian L.
dc.contributor.authorTanner, Justin R.
dc.contributor.authorMcKell, Allison O.
dc.contributor.authorBoudreaux, Crystal E.
dc.contributor.authorDukes, Madeline J.
dc.contributor.authorMcDonald, Sarah M.
dc.contributor.authorKelly, Deborah F.
dc.date.accessioned2013-06-24T19:41:28Z
dc.date.available2013-06-24T19:41:28Z
dc.date.issued2013-03
dc.identifier.issn2072-666X
dc.identifier.urihttp://hdl.handle.net/10919/23260
dc.description13 p.en_US
dc.description.abstractHere we present new applications for silicon nitride (SiN) membranes to evaluate biological processes. We determined that 50-nanometer thin films of SiN produced from silicon wafers were sufficiently durable to bind active rotavirus assemblies. A direct comparison of SiN microchips with conventional carbon support films indicated that SiN performs equivalent to the traditional substrate to prepare samples for Electron Microscopy (EM) imaging. Likewise, SiN films coated with Ni-NTA affinity layers concentrated rotavirus particles similarly to affinity-coated carbon films. However, affinity-coated SiN membranes outperformed glow-discharged conventional carbon films 5-fold as indicated by the number of viral particles quantified in EM images. In addition, we were able to recapitulate viral uncoating and transcription mechanisms directed onto the microchip surfaces. EM images of these processes revealed the production of RNA transcripts emerging from active rotavirus complexes. These results were confirmed by the functional incorporation of radiolabeled nucleotides into the nascent RNA transcripts. Collectively, we demonstrate new uses for SiN membranes to perform molecular surveillance on life processes in real-time.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherMDPI
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectsilicon nitrideen_US
dc.subjectmicrochipsen_US
dc.subjectrotavirusen_US
dc.subjectRNAen_US
dc.subjectelectron microscopyen_US
dc.titleMolecular Surveillance of Viral Processes Using Silicon Nitride Membranesen_US
dc.typeArticle - Refereeden_US
dc.title.serialMicromachines
dc.identifier.doihttps://doi.org/10.3390/mi4010090
dc.type.dcmitypeTexten_US
dc.identifier.sourceurlhttp://www.mdpi.com/2072-666X/4/1/90


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Creative Commons Attribution 4.0 International (CC BY 4.0)
License: Creative Commons Attribution 4.0 International (CC BY 4.0)