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  • De novo formation of an aggregation pheromone precursor by an isoprenyl diphosphate synthase-related terpene synthase in the harlequin bug 

    Lancaster, Jason; Khrimian, Ashot; Young, Sharon; Lehner, Bryan; Luck, Katrin; Wallingford, Anna; Ghosh, Saikat Kumar B.; Zerbe, Philipp; Muchlinski, Andrew; Marek, Paul E.; Sparks, Michael E.; Tokuhisa, James G.; Tittiger, Claus; Koellner, Tobias G.; Weber, Donald C.; Gundersen-Rindal, Dawn E.; Kuhar, Thomas P.; Tholl, Dorothea (2018-09-11)
    Insects use a diverse array of specialized terpene metabolites as pheromones in intraspecific interactions. In contrast to plants and microbes, which employ enzymes called terpene synthases (TPSs) to synthesize terpene ...
  • Improved reference genome of Aedes aegypti informs arbovirus vector control 

    Matthews, Benjamin J.; Dudchenko, Olga; Kingan, Sarah B.; Koren, Sergey; Antoshechkin, Igor; Crawford, Jacob E.; Glassford, William J.; Herre, Margaret; Redmond, Seth N.; Rose, Noah H.; Weedall, Gareth D.; Wu, Yang; Batra, Sanjit S.; Brito-Sierra, Carlos A.; Buckingham, Steven D.; Campbell, Corey L.; Chan, Saki; Cox, Eric; Evans, Benjamin R.; Fansiri, Thanyalak; Filipovic, Igor; Fontaine, Albin; Gloria-Soria, Andrea; Hall, Richard; Joardar, Vinita S.; Jones, Andrew K.; Kay, Raissa G. G.; Kodali, Vamsi K.; Lee, Joyce; Lycett, Gareth J.; Mitchell, Sara N.; Muehling, Jill; Murphy, Michael R.; Omer, Arina D.; Partridge, Frederick A.; Peluso, Paul; Aiden, Aviva Presser; Ramasamy, Vidya; Rasic, Gordana; Roy, Sourav; Saavedra-Rodriguez, Karla; Sharan, Shruti; Sharma, Atashi; Smith, Melissa Laird; Turner, Joe; Weakley, Allison M.; Zhao, Zhilei; Akbari, Omar S.; Black, William C.; Cao, Han; Darby, Alistair C.; Hill, Catherine A.; Johnston, J. Spencer; Murphy, Terence D.; Raikhel, Alexander S.; Sattelle, David B.; Sharakhov, Igor V.; White, Bradley J.; Zhao, Li; Aiden, Erez Lieberman; Mann, Richard S.; Lambrechts, Louis; Powell, Jeffrey R.; Sharakhova, Maria V.; Tu, Zhijian; Robertson, Hugh M.; McBride, Carolyn S.; Hastic, Alex R.; Korlach, Jonas; Neafsey, Daniel E.; Phillippy, Adam M.; Vosshall, Leslie B. (2018-11-22)
    Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and ...
  • Sequential and differential interaction of assembly factors during nitrogenase MoFe protein maturation 

    Jimenez-Vicente, Emilio; Yang, Zhi-Yong; Keith Ray, W.; Echavarri-Erasun, Carlos; Cash, Valerie L.; Rubio, Luis M.; Seefeldt, Lance C.; Dean, Dennis R. (2018-06-22)
    Nitrogenases reduce atmospheric nitrogen, yielding the basic inorganic molecule ammonia. The nitrogenase MoFe protein contains two cofactors, a [7Fe-9S-Mo-C-homocitrate] active-site species, designated FeMo-cofactor, and ...
  • Structuring and Supporting Excellence in Undergraduate Biochemistry and Molecular Biology Education: The ASBMB Degree Accreditation Program 

    Moore, Victoria Del Gaizo; Loertscher, Jennifer; Dean, Diane M.; Bailey, Cheryl P.; Kennelly, Peter J.; Wolfson, Adele J. (2018-12-01)
  • The NifZ accessory protein has an equivalent function in maturation of both nitrogenase MoFe protein P-clusters 

    Jimenez-Vicente, Emilio; Yang, Zhi-Yong; del Campo, Julia S. Martin; Cash, Valerie L.; Seefeldt, Lance C.; Dean, Dennis R. (2019-04-19)
    The Mo-dependent nitrogenase comprises two interacting components called the Fe protein and the MoFe protein. The MoFe protein is an (22) heterotetramer that harbors two types of complex metalloclusters, both of which are ...
  • The Lyme disease spirochete's BpuR DNA/RNA-binding protein is differentially expressed during the mammal-tick infectious cycle, which affects translation of the SodA superoxide dismutase 

    Jutras, Brandon L.; Savage, Christina R.; Arnold, William K.; Lethbridge, Kathryn G.; Carroll, Dustin W.; Tilly, Kit; Bestor, Aaron; Zhu, Haining; Seshu, Janakiram; Zuckert, Wolfram R.; Stewart, Philip E.; Rosa, Patricia A.; Brissette, Catherine A.; Stevenson, Brian (2019-07)
    When the Lyme disease spirochete, Borrelia burgdorferi, transfers from a feeding tick into a human or other vertebrate host, the bacterium produces vertebrate-specific proteins and represses factors needed for arthropod ...
  • Two of the three Transformer-2 genes are required for ovarian development in Aedes albopictus 

    Li, Xiaocong; Jin, Binbin; Dong, Yunqiao; Chen, Xiaoguang; Tu, Zhijian; Gu, Jinbao (2019-06)
    In Drosophila melanogaster, transformer-2 (tra2) plays an essential role in the sex-specific splicing of doublesex (dsx) and fruitless (fru), two key transcription factor genes that program sexual differentiation and ...
  • Comparison of Common Analytical Methods for the Quantification of Total Polyphenols and Flavanols in Fruit Juices and Ciders 

    Ma, Sihui; Kim, Cathlean; Neilson, Andrew P.; Griffin, Laura E.; Peck, Gregory M.; O'Keefe, Sean F.; Stewart, Amanda C. (2019-07-17)
    Multiple analytical methods are used for quantification of total polyphenols and total flavanols in fruit juices and beverages. Four methods were evaluated in this study: Folin-Ciocalteu (F-C), Lowenthal permanganate (L-P), ...
  • Internalization of Erythrocyte Acylpeptide Hydrolase Is Required for Asexual Replication of Plasmodium falciparum 

    Elahi, Rubayet; Dapper, Christie; Klemba, Michael (American Society for Microbiology, 2019-05-08)
    The human malaria parasite Plasmodium falciparum causes disease as it replicates within the host's erythrocytes. We have found that an erythrocyte serine hydrolase, acylpeptide hydrolase (APEH), accumulates within developing ...
  • Expression analysis and knockdown of two antennal odorant-binding protein genes in Aedes aegypti 

    Sengul, Meryem S.; Tu, Zhijian (Entomological Society of America, 2010-10-05)
    The presence and expression of odorant-binding proteins (OBPs) in the olfactory organs suggest that they play an important role in mosquito olfaction. However, no direct evidence has been found for their involvement in the ...
  • Odorant receptor c-terminal motifs in divergent insect species 

    Miller, Raymond; Tu, Zhijian (Entomological Society of America, 2008-09-22)
    Insect odorant receptors are a large family of seven transmembrane proteins believed to be G-protein coupled receptors. The peptide sequences of two odorant receptors within a given species may share as little as 17% ...
  • Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids 

    Vavricka, Christopher J.; Yoshida, Takanobu; Kuriya, Yuki; Takahashi, Shunsuke; Ogawa, Teppei; Ono, Fumie; Agari, Kazuko; Kiyota, Hiromasa; Li, Jianyong; Ishii, Jun; Tsuge, Kenji; Minami, Hiromichi; Araki, Michihiro; Hasunuma, Tomohisa; Kondo, Akihiko (Springer Nature, 2019-05-01)
    Previous studies have utilized monoamine oxidase (MAO) and L-3,4-dihydroxyphenylalanine decarboxylase (DDC) for microbe-based production of tetrahydropapaveroline (THP), a benzylisoquinoline alkaloid (BIA) precursor to ...
  • Complete Genome Sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286 

    Umaña, Ariana; Lemkul, Justin A.; Slade, Daniel J. (American Society for Microbiology, 2019-02-21)
    Fusobacterium necrophorum is a pathogenic Gram-negative, anaerobic bacterium. In this study, we present the first complete genome sequence of Fusobacterium necrophorum subsp. necrophorum ATCC 25286. These data provide a ...
  • Borrelia burgdorferi peptidoglycan is a persistent antigen in patients with Lyme arthritis 

    Jutras, Brandon L.; Lochhead, Robert B.; Kloos, Zachary A.; Biboy, Jacob; Strle, Klemen; Booth, Carmen J.; Govers, Sander K.; Gray, Joe; Schumann, Peter; Vollmer, Waldemar; Bockenstedt, Linda K.; Steere, Allen C.; Jacobs-Wagner, Christine (National Academy of Sciences, 2019-06-17)
    Lyme disease is a multisystem disorder caused by the spirochete Borrelia burgdorferi. A common late-stage complication of this disease is oligoarticular arthritis, often involving the knee. In ∼10% of cases, arthritis ...
  • Identification of aaNAT5b as a spermine N-acetyltransferase in the mosquito, Aedes aegypti 

    Guan, Huai; Wang, Maoying; Liao, Chenghong; Liang, Jing; Mehere, Prajwalini; Tian, Meiling; Liu, Hairong; Robinson, Howard; Li, Jianyong; Han, Qian (PLOS, 2018-03-19)
    Mosquitoes transmit a number of diseases in animals and humans, including Dengue, Chikungunya and Zika viruses that affect millions of people each year. Controlling the disease-transmitting mosquitoes has proven to be a ...
  • Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS 

    Whiten, Shavonn R.; Ray, W. Keith; Helm, Richard F.; Adelman, Zach N. (PLOS, 2018-03-23)
    The Aedes aegypti mosquito is the principal vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus. These arboviruses are transmitted during adult female mosquito bloodfeeding. While these viruses ...
  • Engel Hall News, Winter 2018 

    Unknown author (Biochemistry Dept. Virginia Tech., 2018)
    This newsletter highlights events, new faculty, and student research in the Department of Biochemistry at Virginia Tech.
  • A Pterin-Dependent Signaling Pathway Regulates a Dual-Function Diguanylate Cyclase-Phosphodiesterase Controlling Surface Attachment in Agrobacterium tumefaciens 

    Feirer, Nathan; Xu, Jing; Allen, Kylie D.; Koestler, Benjamin J.; Bruger, Eric L.; Waters, Christopher M.; White, Robert H.; Fuqua, Clay (American Society for Microbiology, 2015-07)
    The motile-to-sessile transition is an important lifestyle switch in diverse bacteria and is often regulated by the intracellular second messenger cyclic diguanylate monophosphate (c-di-GMP). In general, high c-di-GMP ...
  • Diversity and Functional Analysis of the FeMo-Cofactor Maturase NifB 

    Arragain, Simon; Jimenez-Vicente, Emilio; Scandurra, Alessandro A.; Buren, Stefan; Rubio, Luis M.; Echavarri-Erasun, Carlos (2017-11-14)
    One of the main hurdles to engineer nitrogenase in a non-diazotrophic host is achieving NifB activity. NifB is an extremely unstable and oxygen sensitive protein that catalyzes a low-potential SAM-radical dependent reaction. ...
  • Comparative Genomics and Proteomic Analysis of Assimilatory Sulfate Reduction Pathways in Anaerobic Methanotrophic Archaea 

    Yu, Hang; Susanti, Dwi; McGlynn, Shawn E.; Skennerton, Connor T.; Chourey, Karuna; Iyer, Ramsunder; Scheller, Silvan; Tavormina, Patricia L.; Hettich, Robert L.; Mukhopadhyay, Biswarup; Orphan, Victoria J. (Frontiers, 2018-12-03)
    Sulfate is the predominant electron acceptor for anaerobic oxidation of methane (AOM) in marine sediments. This process is carried out by a syntrophic consortium of anaerobic methanotrophic archaea (ANME) and sulfate ...

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