Collections in this community

Recent Submissions

  • Borrelia burgdorferi peptidoglycan is a persistent antigen in patients with Lyme arthritis 

    Jutras, Brandon L.; Lochhead, Robert B.; Kloos, Zachary A.; Biboy, Jacob; Strle, Klemen; Booth, Carmen J.; Govers, Sander K.; Gray, Joe; Schumann, Peter; Vollmer, Waldemar; Bockenstedt, Linda K.; Steere, Allen C.; Jacobs-Wagner, Christine (National Academy of Sciences, 2019-06-17)
    Lyme disease is a multisystem disorder caused by the spirochete Borrelia burgdorferi. A common late-stage complication of this disease is oligoarticular arthritis, often involving the knee. In ∼10% of cases, arthritis ...
  • Identification of aaNAT5b as a spermine N-acetyltransferase in the mosquito, Aedes aegypti 

    Guan, Huai; Wang, Maoying; Liao, Chenghong; Liang, Jing; Mehere, Prajwalini; Tian, Meiling; Liu, Hairong; Robinson, Howard; Li, Jianyong; Han, Qian (PLOS, 2018-03-19)
    Mosquitoes transmit a number of diseases in animals and humans, including Dengue, Chikungunya and Zika viruses that affect millions of people each year. Controlling the disease-transmitting mosquitoes has proven to be a ...
  • Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS 

    Whiten, Shavonn R.; Ray, W. Keith; Helm, Richard F.; Adelman, Zach N. (PLOS, 2018-03-23)
    The Aedes aegypti mosquito is the principal vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus. These arboviruses are transmitted during adult female mosquito bloodfeeding. While these viruses ...
  • Engel Hall News, Winter 2018 

    Unknown author (Biochemistry Dept. Virginia Tech., 2018)
    This newsletter highlights events, new faculty, and student research in the Department of Biochemistry at Virginia Tech.
  • A Pterin-Dependent Signaling Pathway Regulates a Dual-Function Diguanylate Cyclase-Phosphodiesterase Controlling Surface Attachment in Agrobacterium tumefaciens 

    Feirer, Nathan; Xu, Jing; Allen, Kylie D.; Koestler, Benjamin J.; Bruger, Eric L.; Waters, Christopher M.; White, Robert H.; Fuqua, Clay (American Society for Microbiology, 2015-07)
    The motile-to-sessile transition is an important lifestyle switch in diverse bacteria and is often regulated by the intracellular second messenger cyclic diguanylate monophosphate (c-di-GMP). In general, high c-di-GMP ...
  • Diversity and Functional Analysis of the FeMo-Cofactor Maturase NifB 

    Arragain, Simon; Jimenez-Vicente, Emilio; Scandurra, Alessandro A.; Buren, Stefan; Rubio, Luis M.; Echavarri-Erasun, Carlos (2017-11-14)
    One of the main hurdles to engineer nitrogenase in a non-diazotrophic host is achieving NifB activity. NifB is an extremely unstable and oxygen sensitive protein that catalyzes a low-potential SAM-radical dependent reaction. ...
  • Comparative Genomics and Proteomic Analysis of Assimilatory Sulfate Reduction Pathways in Anaerobic Methanotrophic Archaea 

    Yu, Hang; Susanti, Dwi; McGlynn, Shawn E.; Skennerton, Connor T.; Chourey, Karuna; Iyer, Ramsunder; Scheller, Silvan; Tavormina, Patricia L.; Hettich, Robert L.; Mukhopadhyay, Biswarup; Orphan, Victoria J. (Frontiers, 2018-12-03)
    Sulfate is the predominant electron acceptor for anaerobic oxidation of methane (AOM) in marine sediments. This process is carried out by a syntrophic consortium of anaerobic methanotrophic archaea (ANME) and sulfate ...
  • Eubacterial SpoVG Homologs Constitute a New Family of Site-Specific DNA-Binding Proteins 

    Jutras, Brandon L.; Chenail, Alicia M.; Rowland, Christi L.; Carroll, Dustin; Miller, M. Clarke; Bykowski, Tomasz; Stevenson, Brian (PLOS, 2013-06-20)
    A site-specific DNA-binding protein was purified from Borrelia burgdorferi cytoplasmic extracts, and determined to be a member of the highly conserved SpoVG family. This is the first time a function has been attributed to ...
  • BpaB, a novel protein encoded by the Lyme disease spirochete’s cp32 prophages, binds to erp Operator 2 DNA 

    Burns, Logan H.; Adams, Claire A.; Riley, Sean P.; Jutras, Brandon L.; Bowman, Amy; Chenail, Alicia M.; Cooley, Anne E.; Haselhorst, Laura A.; Moore, Alisha M.; Babb, Kelly; Fried, Michael G.; Stevenson, Brian (Oxford University Press, 2010)
    Borrelia burgdorferi produces Erp outer surface proteins throughout mammalian infection, but represses their synthesis during colonization of vector ticks. A DNA region 50 of the start of erp transcription, Operator 2, was ...
  • Genomic insights into the Ixodes scapularis tick vector of Lyme disease 

    Gulia-Nuss, Monika; Nuss, Andrew B.; Meyer, Jason M.; Sonenshine, Daniel E.; Roe, R. Michael; Waterhouse, Robert M.; Sattelle, David B.; de la Fuente, Jose; Ribeiro, Jose M.; Megy, Karine; Thimmapuram, Jyothi; Miller, Jason R.; Walenz, Brian P.; Koren, Sergey; Hostetler, Jessica B.; Thiagarajan, Mathangi; Joardar, Vinita S.; Hannick, Linda I.; Bidwell, Shelby; Hammond, Martin P.; Young, Sarah; Zeng, Qiandong; Abrudan, Jenica L.; Almeida, Francisca C.; Ayllon, Nieves; Bhide, Ketaki; Bissinger, Brooke W.; Bonzon-Kulichenko, Elena; Buckingham, Steven D.; Caffrey, Daniel R.; Caimano, Melissa J.; Croset, Vincent; Driscoll, Timothy; Gilbert, Don; Gillespie, Joseph J.; Giraldo-Calderon, Gloria I.; Grabowski, Jeffrey M.; Jiang, David; Khalil, Sayed M. S.; Kim, Donghun; Kocan, Katherine M.; Koci, Juraj; Kuhn, Richard J.; Kurtti, Timothy J.; Lees, Kristin; Lang, Emma G.; Kennedy, Ryan C.; Kwon, Hyeogsun; Perera, Rushika; Qi, Yumin; Radolf, Justin D.; Sakamoto, Joyce M.; Sanchez-Gracia, Alejandro; Severo, Maiara S.; Silverman, Neal; Simo, Ladislav; Tojo, Marta; Tornador, Cristian; Van Zee, Janice P.; Vazquez, Jesus; Vieira, Filipe G.; Villar, Margarita; Wespiser, Adam R.; Yang, Yunlong; Zhu, Jiwei; Arensburger, Peter; Pietrantonio, Patricia V.; Barker, Stephen C.; Shao, Renfu; Zdobnov, Evgeny M.; Hauser, Frank; Grimmelikhuijzen, Cornelis J. P.; Park, Yoonseong; Rozas, Julio; Benton, Richard; Pedra, Joao H. F.; Nelson, David R.; Unger, Maria F.; Tubio, Jose M. C.; Tu, Zhijian; Robertson, Hugh M.; Shumway, Martin; Sutton, Granger; Wortman, Jennifer R.; Lawson, Daniel; Wikel, Stephen K.; Nene, Vishvanath M.; Fraser, Claire M.; Collins, Frank H.; Birren, Bruce; Nelson, Karen E.; Caler, Elisabet; Hill, Catherine A. (Springer Nature, 2016-02)
    Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic ...
  • Early preclinical detection of prions in the skin of prion-infected animals 

    Wang, Zerui; Manca, Matteo; Foutz, Aaron; Camacho, Manuel V.; Raymond, Gregory J.; Race, Brent; Orru, Christina D.; Yuan, Jue; Shen, Pingping; Li, Baiya; Lang, Yue; Dang, Johnny; Adornato, Alise; Williams, Katie; Maurer, Nicholas R.; Gambetti, Pierluigi; Xu, Bin; Surewicz, Witold; Petersen, Robert B.; Dong, Xiaoping; Appleby, Brian S.; Caughey, Byron; Cui, Li; Kong, Qingzhong; Zou, Wen-Quan (2019-01-16)
    A definitive pre-mortem diagnosis of prion disease depends on brain biopsy for prion detection currently and no validated alternative preclinical diagnostic tests have been reported to date. To determine the feasibility ...
  • Identification of MOS9 as an interaction partner for chalcone synthase in the nucleus 

    Watkinson, Jonathan I.; Bowerman, Peter A.; Crosby, Kevin C.; Hildreth, Sherry B.; Helm, Richard F.; Winkel, Brenda S.J. (PeerJ, 2018-09-19)
    Plant flavonoid metabolism has served as a platform for understanding a range of fundamental biological phenomena, including providing some of the early insights into the subcellular organization of metabolism. Evidence ...
  • The Lyme disease spirochete’s BpuR DNA- / RNA-binding protein is differentially expressed during the mammal-tick infectious cycle, and affects translation of the SodA superoxide dismutase 

    Jutras, Brandon L.; Arnold, William; Savage, Christina; Tilly, Kit; Beastor, Aaron; Rosa, Patti; Brisette, Catherin; Stevenson, Brian
  • Borrelia burgdorferi SpoVG DNA- and RNA-Binding Protein Modulates the Physiology of the Lyme Disease Spirochete 

    Savage, Christina R.; Jutras, Brandon L.; Bestor, Aaron; Tilly, Kit; Rosa, Patricia A.; Tourand, Yvonne; Stewart, Philip E.; Brissette, Catherine A.; Stevenson, Brian (AMER SOC MICROBIOLOGY, 2018-06-01)
  • Juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes 

    Liu, Pengcheng; Fu, Xiaonan; Zhu, Jinsong (NATL ACAD SCIENCES, 2018-08-14)
    Juvenile hormone (JH) regulates many aspects of insect development and reproduction. In some processes, JH plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20E). In Aedes aegypti ...
  • Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti 

    Hu, Wanqi; Tu, Zhijian J (2018-12-24)
    Abstract Background Aedes aegypti is an important mosquito vector that transmits arboviruses that cause devastating diseases including Zika, dengue fever, yellow fever and ...
  • Pure early zygotic genes in the Asian malaria mosquito Anopheles stephensi 

    Wu, Yang; Hu, Wanqi; Biedler, James K; Chen, Xiao-Guang; Tu, Zhijian J (2018-12-24)
    Abstract Background The Asian malaria mosquito, Anopheles stephensi, is a major urban malaria vector in the Middle East and on the Indian subcontinent. Early zygotic transcription, ...
  • Broccoli Fluorets: Split Aptamers as a User-Friendly Fluorescent Toolkit for Dynamic RNA Nanotechnology 

    Chandler, Morgan; Lyalina, Tatiana; Halman, Justin; Rackley, Lauren; Lee, Lauren; Dang, Dylan; Ke, Weina; Sajja, Sameer; Woods, Steven; Acharya, Shrija; Baumgarten, Elijah; Christopher, Jonathan; Elshalia, Emman; Hrebien, Gabriel; Kublank, Kinzey; Saleh, Saja; Stallings, Bailey; Tafere, Michael; Striplin, Caryn; Afonin, Kirill A. (MDPI, 2018-12-02)
    RNA aptamers selected to bind fluorophores and activate their fluorescence offer a simple and modular way to visualize native RNAs in cells. Split aptamers which are inactive until the halves are brought within close ...

View more