Now showing items 1-6 of 6
Focusing of mammalian cells under an ultrahigh pH gradient created by unidirectional electropulsation in a confined microchamber
(The Royal Society of Chemistry, 2014-06-09)
The transport and manipulation of cells in microfluidic structures are often critically required in cellular analysis. Cells typically make consistent movement in a dc electric field in a single direction, due to their ...
Low-input and multiplexed microfluidic assay reveals epigenomic variation across cerebellum and prefrontal cortex
Extensive effort is under way to survey the epigenomic landscape of primary ex vivo tissues to establish normal reference data and to discern variation associated with disease. The low abundance of some tissue types and ...
On-chip manufacturing of synthetic proteins for point-of-care therapeutics
Therapeutic proteins have recently received increasing attention because of their clinical potential. Currently, most therapeutic proteins are produced on a large scale using various cell culture systems. However, storing ...
Immunomagnetic separation of tumor initiating cells by screening two surface markers
(Springer Nature, 2017-01-11)
Isolating tumor initiating cells (TICs) often requires screening of multiple surface markers, sometimes with opposite preferences. This creates a challenge for using bead-based immunomagnetic separation (IMS) that typically ...
Microfluidic Electroporation for Cellular Analysis and Delivery
(The Royal Society of Chemistry, 2013-07-08)
Electroporation is a simple yet powerful technique for breaching the cell membrane barrier. The applications of electroporation can be generally divided into two categories: the release of intracellular proteins, nucleic ...
BRCA1 mutations attenuate super-enhancer function and chromatin looping in haploinsufficient human breast epithelial cells
Abstract Background BRCA1-associated breast cancer originates from luminal progenitor cells. BRCA1 functions in multiple biological processes, including double-strand break ...