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dc.contributorVirginia Tech
dc.contributor.authorElgart, V.
dc.contributor.authorJia, T.
dc.contributor.authorKulkarni, R.
dc.date.accessioned2014-02-26T19:10:04Z
dc.date.available2014-02-26T19:10:04Z
dc.date.issued2010-06
dc.identifier.citationElgart, Vlad; Jia, Tao; Kulkarni, Rahul. "Quantifying mRNA Synthesis and Decay Rates Using Small RNAs," Biophysical Journal 98(12), 2780-2784 (2010); doi: 10.1016/j.bpj.2010.03.022
dc.identifier.issn0006-3495
dc.identifier.urihttp://hdl.handle.net/10919/25771
dc.description.abstractRegulation of mRNA decay is a critical component of global cellular adaptation to changing environments. The corresponding changes in mRNA lifetimes can be coordinated with changes in mRNA transcription rates to fine-tune gene expression. Current approaches for measuring mRNA lifetimes can give rise to secondary effects due to transcription inhibition and require separate experiments to estimate changes in mRNA transcription rates. Here, we propose an approach for simultaneous determination of changes in mRNA transcription rate and lifetime using regulatory small RNAs (sRNAs) to control mRNA decay. We analyze a stochastic model for coupled degradation of mRNAs and sRNAs and derive exact results connecting RNA lifetimes and transcription rates to mean abundances. The results obtained are then generalized to include nonstoichiometric coupled degradation of sRNAs. Our analysis suggests experimental protocols for determining parameters controlling the efficiency of stoichiometric regulation by small RNAs and for analyzing factors and processes regulating changes in mRNA transcription and decay.
dc.language.isoen_US
dc.publisherCELL PRESS
dc.subjectstochastic gene-expression
dc.subjectescherichia-coli
dc.subjectregulatory rnas
dc.subjectdegradation
dc.subjectbacteria
dc.titleQuantifying mRNA Synthesis and Decay Rates Using Small RNAs
dc.typeArticle
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006349510003541
dc.date.accessed2014-02-05
dc.title.serialBiophysical Journal
dc.identifier.doihttps://doi.org/10.1016/j.bpj.2010.03.022


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