VTechWorks staff will be away for the Thanksgiving holiday beginning at noon on Wednesday, November 22, through Friday, November 24, and will not be replying to requests during this time. Thank you for your patience, and happy holidays!
Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran
A plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation. (C) 2013 Optical Society of America