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dc.contributor.authorDegener, Arthur W. Jr.en
dc.date.accessioned2014-03-14T20:10:34Zen
dc.date.available2014-03-14T20:10:34Zen
dc.date.issued1999-04-08en
dc.identifier.otheretd-042399-164339en
dc.identifier.urihttp://hdl.handle.net/10919/27183en
dc.description.abstractWith the increased demands of therapeutic proteins, there is going to be a need for new purification technologies which have high throughput, high yield and high resolution. Three purification technologies were explored as potential new technology to isolate recombinant and endogenous milk proteins: Expanded bed adsorption chromatography(EBAC) combined with hydrophobic interaction chromatography(HIC), Recycle continuous flow electrophoresis(RCFE) and Free flow isoelectric focusing(FFIEF). The first process(EBAC/HIC) used with Zn2+ as a selective precipitating agent, purified recombinant human protein C(rhPC) and IgG(contaminated with less than 1% IgA) from swine milk with high resolution and high yield while processing about 10-20 grams in a single operation. The second process(RCFE) was able to isolate the active sub-populations of rhPC from major milk contaminants( - and -pig casein) as wells as from the inactive sub-populations of rhPC. RCFE was able to process 1.5g total protein per hour on a small scale and is currently being researched to process 1kg total protein per hour. The third and final purification process(FFIEF) sub-fractionated 100mg of immuno-purified rhPC into 50 fractions. The FFIEF was able to produce a linear pH gradient over the range of 3-10 using 2% ampholytes. The fractionated rhPC showed differing degrees of activity that resulted from the -carboxylated glutamic acids and the sialic acids.en
dc.publisherVirginia Techen
dc.relation.haspartthesis.PDFen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectendogenous milk proteinsen
dc.subjectrecombinant proteinsen
dc.subjectfree flow iso-electric focusingen
dc.subjectFree flow electrophoresisen
dc.subjectExpanded bed adsorption chromatographyen
dc.titleDownstream processing of recombinant and endogenous proteins from livestock milken
dc.typeDissertationen
dc.contributor.departmentChemical Engineeringen
dc.description.degreePh. D.en
thesis.degree.namePh. D.en
thesis.degree.leveldoctoralen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.disciplineChemical Engineeringen
dc.contributor.committeechairVelander, William H.en
dc.contributor.committeememberDrohan, William N.en
dc.contributor.committeememberWilliams, Kimberly Forstenen
dc.contributor.committeememberConger, William L.en
dc.contributor.committeememberDavis, Richey M.en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-042399-164339/en
dc.date.sdate1999-04-23en
dc.date.rdate2000-04-29en
dc.date.adate1999-04-29en


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