Trace metal effects on ectomycorrhizal growth, diversity, and colonization of host seedlings
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The second experiment examined the adsorption of copper and zinc to acidic Uchee fine loamy sand. Contrary to expectations, the soil adsorbed up to 667 ppm Cu and 238 ppm Zn. Adsorption occurred mainly in the non-crystalline fraction of the soil. This analysis is a new approach in mycorrhizal research, and the crucial need for such tactics is discussed.
The third experiment surveyed ectomycorrhizae on a mine reclamation project in Wise County, Virginia. Pinus strobus trees planted 1, 8, 13, and 25 years prior to the experiment were sampled. Colonization was lower than in well developed soils, but occurred on all seedlings. Increased colonization and a late stage mycobiont (Tuber) occurred on roots taken from the 25 year old subsite. A new observation was made of Suillus americanus on one year old seedlings. Lack of species overlap among sites suggests localized inoculum sources.
The last experiment explored Pinus strobus and Pinus virginiana seedlings naturally regenerating on acidic, bare-mineral soil exposed by a road cut in Floyd County, Virginia. Ectomycorrhizal colonization ranged between 30 to 80 percent. Wide variation among individual samples suggests patchy inoculum distribution. Scleroderma citrinum, a common early-stage fungus, was dominant throughout. Other early stage genera included Rhizopogon, Pisolithus, and Thelephora. Mid to late stage genera including Suillus and Lactarius were identified. Cenococcum, often a dominant taxon, was a minor taxon here. The unusual presence of the ericoid mycobionts Hymenoscyphus and Oidiodendron is discussed. These results suggest that native inoculum can be an important resource for seedling recruitment.
- Doctoral Dissertations