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dc.contributor.authorTanner, Justin Rogersen_US
dc.date.accessioned2014-03-14T20:20:08Z
dc.date.available2014-03-14T20:20:08Z
dc.date.issued2010-10-27en_US
dc.identifier.otheretd-12102010-104722en_US
dc.identifier.urihttp://hdl.handle.net/10919/30038
dc.description.abstractThe need for new antibiotics has been highlighted recently with the increasing pace of emergence of drug resistant pathogens (MRSA, XDR-TB, etc.). Modification of existing antibiotics with the additions of side chains or other chemical groups and genomics based drug targeting have been the preferred method of drug development at the corporate level in recent years. These approaches have yielded few viable antibiotics and natural products are once again becoming an area of interest for drug discovery. We examined the antimicrobial â Red Soilsâ of the Hashemite Kingdom of Jordan that have historically been used to prevent infection and cure rashes by the native peoples. Antimicrobial producing bacteria were present in these soils and found to be the reason for their antibiotic activity. After isolation, these bacteria were found to excrete their antimicrobials into the liquid culture media which we could then attempt to isolate for further study. Adsorbent resins were employed to capture the antimicrobial compounds and then elute them in a more concentrated solution. As part of a drug discovery program, we sought a way to quickly characterize other soils for potential antibiotic producing bacteria. The community level physiologic profile was examined to determine if this approach would allow for a rapid categorizing of soils based on their probability of containing antimicrobial producing microorganisms. This method proved to have a high level of variability that could not be overcome even after mixing using a commercial blender. The role of these antimicrobial producing bacteria within their natural microbial community has largely been confined to microbe-plant interactions. The role of antimicrobial-producing microorganisms in driving the diversity of their community has not been a focus of considerable study. The potential of an antimicrobial-producing bacterium to act as a driver of diversity was examined using an artificial microbial community based in a sand microcosm. The changes in the microbial assemblage indicate that antimicrobial-producing bacteria may act in an allelopathic manner rather than in a predatory role.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartTanner_JR_D_2010.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectAdsorbent Resinen_US
dc.subjectBiolog Ecoplateâ ¢en_US
dc.subjectKeystone Speciesen_US
dc.subjectNatural Product Drug Discoveryen_US
dc.titleAntimicrobial Producing Bacteria as Agents of Microbial Population Dynamicsen_US
dc.typeDissertationen_US
dc.contributor.departmentBiologyen_US
dc.description.degreePh. D.en_US
thesis.degree.namePh. D.en_US
thesis.degree.leveldoctoralen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineBiologyen_US
dc.contributor.committeechairFalkinham, Joseph O. IIIen_US
dc.contributor.committeememberOberlies, Nicholas H.en_US
dc.contributor.committeememberHagedorn, Charles IIIen_US
dc.contributor.committeememberValett, H. Mauriceen_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12102010-104722/en_US
dc.date.sdate2010-12-10en_US
dc.date.rdate2010-12-10
dc.date.adate2010-12-10en_US


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