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dc.contributor.authorCowing, Brandy Ellenen_US
dc.date.accessioned2014-03-14T20:33:13Z
dc.date.available2014-03-14T20:33:13Z
dc.date.issued2000-04-04en_US
dc.identifier.otheretd-04072000-08550041en_US
dc.identifier.urihttp://hdl.handle.net/10919/31644
dc.description.abstractThe growth of many breast cancers is stimulated by the action of the hormone estrogen. Hormonal therapy used to treat these estrogen-dependent breast cancers acts by interfering with the action of estrogen. Current treatments, such as tamoxifen, are not consistently useful due to development of resistance to these drugs. Tamoxifen treatment can also lead to the development of other gynecological cancers, therefore the discovery of novel treatment options for breast cancer is critical. Vitamin B6 is well documented for its role as a modulator of steroid hormones. Pyridoxal phosphate (PLP), the active form of Vitamin B6, may interfere with the action of the estrogen receptor (ER) by blocking the hormone-binding and/or DNA-binding site of the ER. The objective of this study was to examine the effects of Vitamin B6 supplementation on cell proliferation and estrogen-dependent gene expression in breast cancer cells. To accomplish this, estrogen-dependent (MCF-7 and T-47D) and estrogen-independent (BT-20) breast cancer cells were grown in medium supplemented with 0,100, or 300 µM pyridoxal (PL) in the absence or presence of 0.01µM estradiol. Cell counts and [3H]-thymidine incorporation into DNA were assessed in all cell lines. The expression of pS2, an estrogen-sensitive gene, was performed using RNA extracted from MCF-7 cells. PL supplementation was found to significantly decrease total cell numbers and DNA synthesis in both the estrogen-dependent (ER+) and -independent (ER-) breast cancer cells, but did not alter the expression of pS2. These results indicate that PL significantly impairs growth of breast cancer cells and may be exerting its effects via a steroid-independent mechanism.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartbrandyellen1.pdfen_US
dc.rightsI hereby grant to Virginia Tech or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.en_US
dc.subjectEstrogenen_US
dc.subjectPyridoxalen_US
dc.subjectVitamin B6en_US
dc.subjectBreast Canceren_US
dc.subjectSteroid Hormoneen_US
dc.subjectMammaryen_US
dc.titleVitamin B6 Decreases Proliferation and DNA Synthesis in Human Mammary Carcinoma Cell Lines In Vitroen_US
dc.typeThesisen_US
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineHuman Nutrition, Foods, and Exerciseen_US
dc.contributor.committeechairDavis, Barbara A.en_US
dc.contributor.committeememberBunce, George Edwinen_US
dc.contributor.committeememberTalmadge, Robert J.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04072000-08550041/en_US
dc.date.sdate2000-04-07en_US
dc.date.rdate2001-04-14
dc.date.adate2000-04-14en_US


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