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dc.contributor.authorVerbiest, Leenen_US
dc.date.accessioned2014-03-14T20:34:13Z
dc.date.available2014-03-14T20:34:13Z
dc.date.issued2000-04-20en_US
dc.identifier.otheretd-04252000-14290005en_US
dc.identifier.urihttp://hdl.handle.net/10919/31887
dc.description.abstractTodayâ s development of novel vaccines stresses the need for edible vaccines that are inexpensive, easily administered and capable of being stored and transported without refrigeration. Without these characteristics, developing countries find it difficult to adopt vaccination as the central strategy for preventing their most devastating diseases. A promising approach is the production of vaccines in plants we commonly consume. Two major obstacles have been encountered in developing vaccines in plants. First, the expression level of foreign antigens tends to be low and second, co-expression of an adjuvant may be required to facilitate an appropriate immune response. Ricin, a plant toxin that survives the human digestive process, has been proven to stimulate an immune response and could therefore serve as a suitable adjuvant. The long-term goal is to produce a vaccine that protects against the disease entamoebic dysentery.

The specific goal of this research was to produce ricin in tobacco as adjuvant for the vaccine. Vectors were constructed that fused the ricin coding sequence to different plant promoters and transgenic tobacco plants were generated by transformation with Agrobacterium tumefaciens. The levels of expression in these transgenic plants were tested using immunoblot assays. Southern blot analysis was performed for the highest expressors of each construct. The enzymatic activity of the tobacco-synthesized ricin was shown using a protein translation inhibition assay. Expression of ricin was also confirmed using transient transformation of hairy root cultures. Future experiments will address the practical use of the tobacco-synthesized ricin as adjuvant, as well as the expression of the ricin B subunit fused to a protective antigen of Entamoeba histolytica in tobacco as edible vaccine.

en_US
dc.publisherVirginia Techen_US
dc.relation.haspartLVtitlepage.pdfen_US
dc.relation.haspartLVvita.doc.pdfen_US
dc.relation.haspartLVREFERENCES.doc.pdfen_US
dc.relation.haspartLVappendix.doc.pdfen_US
dc.relation.haspartLVlisttables.doc.pdfen_US
dc.relation.haspartLVch5.doc.pdfen_US
dc.relation.haspartLVch4.doc.pdfen_US
dc.relation.haspartLVch3.doc.pdfen_US
dc.relation.haspartLVch1.doc.pdfen_US
dc.relation.haspartLVAcknowl.doc.pdfen_US
dc.relation.haspartLVch2.doc.pdfen_US
dc.relation.haspartLVch6.doc.pdfen_US
dc.relation.haspartLVcont.doc.pdfen_US
dc.relation.haspartLVabstract.doc.pdfen_US
dc.relation.haspartLVlistfig.doc.pdfen_US
dc.rightsI hereby grant to Virginia Tech or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.en_US
dc.subjectadjuvanten_US
dc.subjectricinen_US
dc.subjectedible vaccineen_US
dc.subjectEntamoeba histolyticaen_US
dc.titleExpression strategies for plant-based production of a vaccine adjuvanten_US
dc.typeThesisen_US
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplinePlant Pathology, Physiology, and Weed Scienceen_US
dc.contributor.committeechairCramer, Carole L.en_US
dc.contributor.committeememberDenbow, Cynthia J.en_US
dc.contributor.committeememberWinkel, Brenda S. J.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04252000-14290005/en_US
dc.date.sdate2000-04-25en_US
dc.date.rdate2001-04-26
dc.date.adate2000-04-26en_US


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