Show simple item record

dc.contributor.authorTan, Rachel Hsing Hsingen_US
dc.date.accessioned2014-03-14T20:37:01Z
dc.date.available2014-03-14T20:37:01Z
dc.date.issued2008-03-12en_US
dc.identifier.otheretd-05152008-174339en_US
dc.identifier.urihttp://hdl.handle.net/10919/32819
dc.description.abstractMultiple biomarkers of oxidative stress have been measured and used in human medicine to diagnose and monitor airway disease. The purpose of the study was to determine if similar relationships existed between inflammatory and oxidative stress biomarkers in exhaled breath condensate (EBC), bronchoalveolar lavage fluid (BALF), red blood cells, white blood cells, and plasma; and cytokine expression in airway inflammatory cells and mucosal biopsies of RAO-affected horses and their controls. Sixteen horses in pairs were used: 8 non-RAO-affected (controls) and 8 RAO-affected horses. Samples from all horses were collected at remission (S1), during environmental challenge (S2) and at recovery (S3). RAO-affected horses had significant alterations in cellular glutathione peroxidase (cGPx) activity, ascorbic acid and pH in a number of biological samples. Concentrations of 8-isoprostanes, isofurans, amino acids and mRNA expression of interleukin 4 (IL4), gamma interferon (INFγ), inducible nitric oxide synthase (iNOS), extracellular glutathione peroxidase (GPx-3), and cytosolic superoxide dismutase (SOD-1) were not significantly different or were at the limits of detection. Conductivity was measured and assessed as a potential correctional factor for respiratory fluid dilution. The alterations in biomarker concentrations demonstrate that oxidative stress is an important component of airway inflammation in RAO-affected horses. Further research is warranted in the use of biomarkers and the effects of dietary interventions.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartRTanThesis08.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectexhaled breath condensateen_US
dc.subjectbronchoalveolar lavageen_US
dc.subjectglutathione peroxidaseen_US
dc.subjectpHen_US
dc.subject8-isoprostaneen_US
dc.subjectisofuranen_US
dc.subjectamino aciden_US
dc.subjectrecurrent airway obstructionen_US
dc.subjectoxidative stressen_US
dc.titleBiomarkers of oxidative stress and inflammation in biological samples collected from recurrent airway obstruction (RAO)-affected horses and their controls.en_US
dc.typeThesisen_US
dc.contributor.departmentVeterinary Medical Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineVeterinary Medical Sciencesen_US
dc.contributor.committeechairThatcher, Craig D.en_US
dc.contributor.committeememberBuechner-Maxwell, Virginia A.en_US
dc.contributor.committeememberCrisman, Mark Virgilen_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05152008-174339/en_US
dc.date.sdate2008-05-15en_US
dc.date.rdate2008-06-10
dc.date.adate2008-06-10en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record