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dc.contributor.authorvan der Aa, Eveline Mariaen_US
dc.date.accessioned2014-03-14T20:37:40Z
dc.date.available2014-03-14T20:37:40Z
dc.date.issued2010-05-05en_US
dc.identifier.otheretd-05192010-125546en_US
dc.identifier.urihttp://hdl.handle.net/10919/33008
dc.description.abstractWide literature precedence exists for polymers containing electrostatic interactions and polymers containing hydrogen bonding motifs, however the combination of electrostatic and hydrogen bonding interactions is not widely investigated in current literature. Polyelectrolytes containing hydrogen bonding groups are expected to exhibit properties of both classes of supramolecular interactions. A series of adenine- and thyminecontaining PDMAEMA and tert-butyl acrylate copolymers were synthesized to investigate the effect of incorporating hydrogen bonding groups into a polyelectrolyte. Incorporation of the styrenic nucleobases significantly affected the solubility of these copolymers on aqueous solutions and showed salt-triggerability with higher contents of these groups. Polyelectrolytes are capable of binding and condensing DNA through electrostatic interactions with the negatively charged phosphate groups of the DNA backbone; however a high degree of cytotoxicity is also often observed for these gene delivery systems. The high level of cytotoxicity is attributed to high degree of cationic character for the polyplexes formed with these systems according to the proton-sponge hypothesis. One method of reducing the overall cationic character for these systems is incorporation of non-electrostatic binding mechanisms such as hydrogen bonding. A series of nucleobase-containing PDMAEMA copolymers were utilized in order to investigate the effect of incorporation of these groups on the cell viability, binding efficiency, and transfection efficiency of PDMAEMA.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartvanderAa_EM_T_2010.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectgene deliveryen_US
dc.subjectwater-solubleen_US
dc.subjecthydrogen bondingen_US
dc.subjectelectrostatic interactionsen_US
dc.subjectnucleobaseen_US
dc.subjectpolyelectrolyteen_US
dc.titleSYNTHESIS AND CHARACTERIZATION OF NUCLEOBASE-CONTAINING POLYELECTROLYTES FOR GENE DELIVERYen_US
dc.typeThesisen_US
dc.contributor.departmentChemistryen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
dc.contributor.committeechairTimothy E. Longen_US
dc.contributor.committeememberTheresa M. Reinekeen_US
dc.contributor.committeememberRobert E. Mooreen_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05192010-125546/en_US
dc.date.sdate2010-05-19en_US
dc.date.rdate2013-05-21
dc.date.adate2010-07-16en_US


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