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dc.contributor.authorFang, Zhenglaien_US

The goal of this project was to design a new kind of tagged supramolecular structural motif. These systems are modular in design and able to bind to DNA. The motif can be represented as TAG-LA-BL-BAS (TAG = NMR active phosphine ligand, LA = light absorber, BL = bridging ligand and BAS = bioactive site). The TAG provides a NMR probe for the characterization of supramolecular complexes as well as for the future investigation of the metal complexâ DNA interaction process. In this project the phosphorous ligand PEt2Ph was selected as the TAG due its ability to provide an easy 31 P NMR probe in the research. The LA represents the light absorber which could be photoexcited by photons of proper energy, here a Ru II (tpy)(PEt2Ph)(BL) chromophore is used. The bridging ligands are those bidentate polyazine ligands that can connect two metal center together in a polymetallic system and have a low energy ð * orbital. The BAS represents the bioactive sites for binding to DNA, in this case the cis-Pt II Cl2 moiety based on previous studies with cisplatin.

The Ru-Pt bimetallic complexes [(tpy)Ru(PEt2Ph)(BL)PtCl2](PF6)2 (BL = bpm or dpp) and their precursors were designed, successfully synthesized and characterized. The synthesis followed a building block approach, allowing variation of the supramolecular system. The final bimetallic complexes were made without need for Al2O3 column chromatograph, important due to the presence of the labile Pt II Cl2 center.

The bimetallic complexes and all of their monometallic precursors were fully characterized by FAB MS, electrochemistry, electronic absorption and 31 P NMR spectroscopy. The Ru-Pt bimetallic complex containing the bpm bridging ligand and its precursors were also characterized by 1 H NMR. The FAB MS spectra of the complexes is characterized by the appearance of the parent ion peaks [M-PF6] + and [M-2PF6] + . The cyclic voltammogram of all complexes show metal based oxidation(s) and ligand based reductions. The electronic absorption spectra of the complexes are characteristic of the lowest lying Ru to bridging ligand MLCT (metal to ligand charge transfer) transition with higher energy bridging and terminal ligand based Pi to Pi* transitions. The electronic absorption data are consistent with the electrochemical data. The 31 P NMR technique provides an efficient and easy characterization method for the complexes, showing the utility of this structural moiety.

The DNA binding activity of the bimetallic complexes were studied by non-denaturing agarose gel electrophoresis and the results show that these tagged bimetallic complexes can bind to DNA through the cis-Pt II Cl2 moiety. This binding has a more pronounced retardation effect on DNA migration than cis-[Pt(NH3)2Cl2] (cisplatin), but less than [Ru(bpy)2(dpq)PtCl2](CF3SO3)2. The DNA binding study establishes these bimetallic complexes with a NMR tag ligand, PEt2Ph, as a new kind of DNA binding agent.

dc.publisherVirginia Techen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.titleThe synthesis and characterization of a new kind of tagged Ru-Pt bimetallic DNA binding agenten_US
dc.contributor.departmentChemistryen_US of Scienceen_US Polytechnic Institute and State Universityen_US
dc.contributor.committeechairBrewer, Karen J.en_US
dc.contributor.committeememberKingston, David G. I.en_US
dc.contributor.committeememberHanson, Brian E.en_US
dc.contributor.committeecochairWinkel, Brenda S. J.en_US

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