Show simple item record

dc.contributor.authorGarrison, Kevin Leeen_US
dc.date.accessioned2014-03-14T20:39:53Z
dc.date.available2014-03-14T20:39:53Z
dc.date.issued2012-06-04en_US
dc.identifier.otheretd-06122012-154308en_US
dc.identifier.urihttp://hdl.handle.net/10919/33542
dc.description.abstractHair cell structures are one of the most common forms of sensing elements found in nature. In humans, approximately 16,000 auditory hair cells can be found in the cochlea of the ear. Each hair cell contains a stereocilia, which is the primary structure for sound transduction. This study looks to develop and characterize a bilayer lipid membrane (BLM) operated artificial hair cell sensor that resembles the stereocilia of the human ear. To develop this sensor, a flexible substrate with internal compartments for hosting the biomolecules and mating cap are constructed and experimentally characterized. The regulated attachment method (RAM) is used to form bilayers within the sealed device. Capacitance measurements of the encapsulated bilayer show that the sealing cap slightly compresses the bottom insert and reduces the size of the enclosed bilayer. Single channel measurements of alamethicin peptides further verify that the encapsulated device can be used to detect the gating activity of transmembrane proteins in the membrane. The flexible substrate was incorporated into a low-noise, portable test fixture. The response of the sensor and tip velocity of the hair were measured with respect to an impulse input on the test fixture and several frequency response functions (FRFs) were created. The FRF between the sensor and the tip velocity was used to show that the hair vibration was transmitted to the bilayer for certain hair lengths. The transfer function between the sensor and the input was used to show the effect of membrane potential on sensor response.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartGarrison_KL_T_2012.pdfen_US
dc.relation.haspartGarrison_KL_T_2012_Copyright1.pdfen_US
dc.relation.haspartGarrison_KL_T_2012_Copyright2.PDFen_US
dc.relation.haspartGarrison_KL_T_2012_Copyright3.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectmembrane-based sensoen_US
dc.subjectphospholipidsen_US
dc.subjectcell membraneen_US
dc.subjecthair cell sensoren_US
dc.subjectbilayer lipid membraneen_US
dc.titleDesign, Fabrication, and Validation of Membrane-Based Sensorsen_US
dc.typeThesisen_US
dc.contributor.departmentMechanical Engineeringen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineMechanical Engineeringen_US
dc.contributor.committeechairLeo, Donald J.en_US
dc.contributor.committeememberGrant, John Wallaceen_US
dc.contributor.committeememberTarazaga, Pablo A.en_US
dc.contributor.committeememberSarles, Stephen A.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06122012-154308/en_US
dc.date.sdate2012-06-12en_US
dc.date.rdate2012-07-13
dc.date.adate2012-07-13en_US


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record