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dc.contributor.authorLeininger, Dagny Jayneen_US
dc.date.accessioned2014-03-14T20:40:02Z
dc.date.available2014-03-14T20:40:02Z
dc.date.issued2001-05-09en_US
dc.identifier.otheretd-06152001-174539en_US
dc.identifier.urihttp://hdl.handle.net/10919/33584
dc.description.abstractDiagnosis techniques and treatments for Escherichia coli mastitis in the dairy bovine were evaluated in two experiments. The first experiment evaluated eosin methylene blue agar as a method of distinguishing E.coli from other gram-negative mastitis pathogens. Escherichia coli will usually produce a green metallic sheen on eosin methylene blue agar. One hundred and twenty-nine milk samples or gram-negative isolates from milk samples were used to compare eosin methylene blue agar to a commercial biochemical test strip (the accepted standard). There was an intermethod agreement of 96.9% and a k-value of 93.7% indicating excellent agreement beyond chance between test methods. Eosin methylene blue agar is a reliable method for differentiation of E. coli from other gram-negative mastitis pathogens. The second experiment evaluated the efficacy of frequent milk-out as a treatment for E. coli mastitis. Sixteen Holstein dairy cows were divided into 2 blocks and randomly assigned to 1 of 4 treatment groups: 1) non-infected, not frequently milked-out, i.e. not treated (NI-NT), 2) experimentally infected with E. coli, not treated (EC-NT), 3) non-infected, frequently milked-out (NI-FMO), and 4) experimentally infected with E. coli, frequently milked-out (EC-FMO). Hours to bacterial, clinical and systemic cure were not different between the EC-NT and EC-FMO treatment groups. Serum a-lactalbumin concentrations were evaluated between treatment groups as a measure of udder health. Serum a-lactalbumin concentrations were higher in cows in the EC-NT treatment group than cows in the NI-NT, NI-FMO and EC-FMO treatment groups at 12 hours post-experimental challenge. Serum a-lactalbumin concentrations were higher in cows in the NI-FMO treatment group than in cows in the NI-NT, EC-NT and EC-FMO treatment groups at 36 hours post-experimental challenge. Results from this study do not support frequent milk-out as a treatment for E. coli mastitis.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartLeiningerThesis.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectMAstitisen_US
dc.subjectEscherichia colien_US
dc.subjectEsoin Methylene BLue Agaren_US
dc.subjectFrequent Milk-outen_US
dc.titleEscherichia coli Mastitis in the Dairy Bovineen_US
dc.typeThesisen_US
dc.contributor.departmentVeterinary Medical Sciencesen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
dc.contributor.committeechairRoberson, Jerry R.en_US
dc.contributor.committeememberElvinger, Francois C.en_US
dc.contributor.committeememberAkers, Robert Michaelen_US
dc.contributor.committeememberHovingh, Ernest P.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06152001-174539/en_US
dc.date.sdate2001-06-15en_US
dc.date.rdate2002-06-28
dc.date.adate2001-06-28en_US


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