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dc.contributor.authorCassidy, Clifton Clarken_US
dc.date.accessioned2010-07-23en_US
dc.date.accessioned2014-03-14T20:40:58Z
dc.date.available2010-07-23en_US
dc.date.available2014-03-14T20:40:58Z
dc.date.issued2010-02-03en_US
dc.date.submitted2010-07-01en_US
dc.identifier.otheretd-07012010-214035en_US
dc.identifier.urihttp://hdl.handle.net/10919/33859
dc.description.abstractBrucella abortus is a zoonotic agent that primarily infects cattle and causes brucellosis. B. abortus strain RB51 is a live, attenuated vaccine licensed for cattle. However, there is no available vaccine to prevent human brucellosis. Outer membrane vesicles have been tested as potential vaccines to prevent diseases caused by bacterial species. OMV are constantly released from Gram-negative bacteria. They are comprised principally of the outer membrane components and periplasmic proteins from the bacterial cell envelope. The research in this thesis examined the adjuvant property of non-replicative, metabolically active irradiated strain RB51 and the protective ability of OMV derived from strain RB51. Irradiated B. abortus strain RB51 was assessed for its ability to act as an adjuvant to induce protection against malaria. It was found that irradiated B. abortus strain RB51 administered along with fasciclin related adhesive protein (FRAP) to mice induced a protective immune response and a significant decrease in parasitemia after challenge with Plasmodium berghei. Strain RB51 and strain RB51 over-producing Cu/Zn superoxide dismutase (Cu/Zn SOD) were used to produce OMV. Western blotting and SDS-PAGE gel staining confirmed the presence of OMV and the over-production of Cu/Zn SOD. OMV were delivered to mice using an intraperitoneal route and, in some cases, with aluminum hydroxide adjuvant. The immune response was assessed by antibody isotyping with respect to OMV and measuring splenic clearance (i.e. protection) from a B. abortus strain 2308 challenge. The results demonstrate that OMV from B. abortus strain RB51 or strain RB51 over producing Cu/Zn SOD produced a Th1 polarized immune response as measured by specific OMV antibodies and cytokines but no statistically significant protection was observed.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartCassidy_CC_T_2010.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectouter membrane vesiclesen_US
dc.subjectstrain RB51en_US
dc.subjectBrucella abortusen_US
dc.subjectadjuvanten_US
dc.subjectblebsen_US
dc.subjectvaccineen_US
dc.subjectmiceen_US
dc.subjectOMVen_US
dc.subjectmalariaen_US
dc.subjectFRAPen_US
dc.titleBrucella abortus Strain RB51 Outer Membrane Vesicles as a Vaccine Against Brucellosis in a Murine Modelen_US
dc.typethesisen_US
dc.contributor.departmentVeterinary Medical Sciencesen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
dc.contributor.committeechairBoyle, Stephen M.en_US
dc.contributor.committeememberMullarky, Isis K.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07012010-214035/en_US
dc.contributor.committeecochairSriranganathan, Nammalwaren_US


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