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dc.contributor.authorSchuchert, Jennifer Annen_US
dc.date.accessioned2014-03-14T20:41:51Z
dc.date.available2014-03-14T20:41:51Z
dc.date.issued2002-06-26en_US
dc.identifier.otheretd-07242002-214305en_US
dc.identifier.urihttp://hdl.handle.net/10919/34135
dc.description.abstractTraditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all serotypes excluding serotype 4 or a 489 bp DNA fragment in all serotypes including serotype 4. Primers specific to the capsular polysaccharide biosynthesis regions of A. pleuropneumoniae serotypes 1, 2, and 8 amplified a 1.6 kb, a 1.7 kb, and 970 bp fragment in the respective serotype. This PCR assay detects A. pleuropneumoniae and identifies serotypes 1, 2, and 8.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartfinalThesis082602.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectMultiplex PCRen_US
dc.subjectserotypingen_US
dc.subjectActinobacillus pleuropneumoniaeen_US
dc.titleDetection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reactionen_US
dc.typeThesisen_US
dc.contributor.departmentVeterinary Medical Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineVeterinary Medical Sciencesen_US
dc.contributor.committeechairInzana, Thomas J.en_US
dc.contributor.committeememberBoyle, Stephen M.en_US
dc.contributor.committeememberSriranganathan, Nammalwaren_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07242002-214305/en_US
dc.date.sdate2002-07-24en_US
dc.date.rdate2003-08-30
dc.date.adate2002-08-30en_US


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