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dc.contributor.authorBeaty, Myron Hen_US
dc.date.accessioned2014-03-14T20:42:08Z
dc.date.available2014-03-14T20:42:08Z
dc.date.issued1991-12-01en_US
dc.identifier.otheretd-07282008-135156en_US
dc.identifier.urihttp://hdl.handle.net/10919/34224
dc.description.abstractIn an attempt to expand the knowledge of the diversity of algal taxa that are capable of successful recovery after freezing to and thawing from -196°C, 365 algal strains representing 13 algal classes were studied using various cryoprotectants at several concentrations. A cryopreservation protocol involving culturing the algae under low light (17-22 uE m-2 s-I) on semisolid (I % agar) media, controlled cooling at 1°C min-I, rapid thawing, and the return of cultures to agar media was developed. This protocol produced successful recovery of >77% (283) of the algal strains tested. Of the 283 successfully cryopreserved strains comprising 240 species (68 genera), >220 species (34 genera) were cryopreserved for the first time. In addition, preliminary data suggest that some relationships exist among proposed systematic evolutionary lines, environmental preferences, cryoprotectants, and the organism's cryopreservability.en_US
dc.format.mediumBTDen_US
dc.publisherVirginia Techen_US
dc.relation.haspartLD5655.V855_1991.B427.pdfen_US
dc.subjectetc.en_US
dc.subjectCryopreservation of organsen_US
dc.subjecttissuesen_US
dc.subject.lccLD5655.V855 1991.B427en_US
dc.titleCryopreservation of eukaryote algaeen_US
dc.typeThesisen_US
dc.contributor.departmentBiologyen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
dc.contributor.committeechairParker, Bruce C.en_US
dc.contributor.committeememberMiller, Orson K. Jr.en_US
dc.contributor.committeememberOrcutt, David M.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07282008-135156/en_US
dc.date.sdate2008-07-28en_US
dc.date.rdate2008-07-28
dc.date.adate2008-07-28en_US


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