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The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa
A series of in vitro studies were conducted to evaluate
the effect of poly-l-lysine concentration, molecular weight,
and encapsulation temperature on the post encapsulation survivability
of spermatozoa. Viability of spermatozoa encapsulated
at 2012 C using four poly-l-lysine concentrations
(.05%, .15%, .25%, and .35%) did not differ over the 8 h incubation
period. However, the viability of each of the four
treatments was lower than that of the unencapsulated control
(p<.05 and p<.01; percentage motility and percentage intact
acrosomes, respectively), indicating spermatozoal damage occurred
during the encapsulation process. Capsule wall
thickness and integrity for the .15%, .25%, and .35% concentrations
were greater (p<.Ol) than that of the .05% capsules.