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dc.contributor.authorGood, Laura Leeen_US
dc.date.accessioned2014-03-14T20:43:06Z
dc.date.available2014-03-14T20:43:06Z
dc.date.issued2003-07-18en_US
dc.identifier.otheretd-08102001-144854en_US
dc.identifier.urihttp://hdl.handle.net/10919/34451
dc.description.abstractThe objective of this research was to isolate genes encoding isoforms of the enzyme D-myo-inositol 3-phosphate synthase (MIPS, E.C. 5.5.1.4) from soybean and to characterize their expression, especially with respect to their involvement in phytic acid biosynthesis. A MIPS-homologous cDNA, designated GmMIPS1, was isolated via PCR using total RNA from developing seeds. Southern blot analysis and examination of MIPS-homologous soybean EST sequences suggested that GmMIPS1 is part of a multigene family of at least four similar members. The sequences of promoter and genomic regions of GmMIPS1 and GmMIPS2 revealed a high degree of sequence conservation. Northern and western blot analyses showed that MIPS transcript and protein are abundantly expressed early in seed development. Immunolocalization of MIPS protein in developing seeds confirmed expression of MIPS early in seed development and correlated MIPS protein accumulation in soybean seed tissue with tissues in which phytic acid is known to accumulate. The promoter region of GmMIPS1 was isolated and analyzed for possible seed-specificity using promoter:GUS fusions. Two GmMIPS1 promoter fragments were capable of conferring GUS expression when bombarded directly into developing soybean seeds. However, preliminary bombardment experiments into soybean cell suspension culture indicated that both promoter fragments drove expression of GUS in undifferentiated tissue, indicating a potential lack of seed-specificity.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartllgetd.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectMIPSen_US
dc.subjectmyo-inositol phosphate synthaseen_US
dc.subjectGlycine maxen_US
dc.subjectphytic aciden_US
dc.subjectsoybean seed developmenten_US
dc.titleIsolation and Characterization of D-Myo-Inositol-3-Phosphate Synthase Gene Family Members in Soybeanen_US
dc.typeThesisen_US
dc.contributor.departmentPlant Pathology, Physiology, and Weed Scienceen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplinePlant Pathology, Physiology, and Weed Scienceen_US
dc.contributor.committeechairGrabau, Elizabeth A.en_US
dc.contributor.committeememberMcDowell, John M.en_US
dc.contributor.committeememberGillaspy, Glenda E.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08102001-144854/en_US
dc.date.sdate2001-08-10en_US
dc.date.rdate2006-10-27
dc.date.adate2001-08-13en_US


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