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dc.contributor.authorRiquelme Breazeal, Maria Virginiaen_US
dc.date.accessioned2014-03-14T20:43:32Z
dc.date.available2014-03-14T20:43:32Z
dc.date.issued2011-08-05en_US
dc.identifier.otheretd-08152011-132615en_US
dc.identifier.urihttp://hdl.handle.net/10919/34564
dc.description.abstractAnthropogenically generated antibiotic resistance genes (ARGs) are considered emerging contaminants, as they are associated with a critical human health challenge, are persist independent of a bacterial host, are subject to transfer between bacteria, and are present at amplified levels in human-impacted environments. Given the gravity of the problem, there is growing interest in advancing water treatment processes capable of limiting ARG dissemination. This study examined the potential for membrane treatment of microconstituent ARGs, and the effect of wastewater colloids on their removal. Native and spiked extracellular vanA (vancomycin resistance) and blaTEM (β-lactam resistance) ARGs were tracked by quantitative polymerase chain reaction through a cascade of membrane filtration steps. To gain insight into potential associations occurring between ARGs and colloidal material, the wastewater colloids were characterized by scanning electron microscopy, as well as in their protein, polysaccharide, and total organic carbon content. The results suggest that extracellular DNA (eDNA) containing ARGs interacts with wastewater colloids, and can both be protected against degradation and be removed more efficiently in the presence of wastewater colloidal material. Thus, ARG removal may be achievable in sustainable water reuse scenarios using lower cost membranes than would have been selected based on molecular size alone. As membranes are likely to play a vital role in water sustainability, the results of this study enable consideration of ARG removal as part of a comprehensive strategy to manage emerging contaminants and to minimize overall public health risks.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartRiquelmeBreazeal_MV_T_2011.pdfen_US
dc.relation.haspartRiquelmeBreazeal_MV_2011_Fairuse.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectUFen_US
dc.subjectnanofiltrationen_US
dc.subjectwastewateren_US
dc.subjectARGsen_US
dc.subjectAntibiotic resistance genesen_US
dc.subjectmicrofiltrationen_US
dc.subjectultrafiltrationen_US
dc.subjectMFen_US
dc.subjectcolloidsen_US
dc.subjectNFen_US
dc.titleEffect of wastewater colloids on membrane removal of microconstituent antibiotic resistance genesen_US
dc.typeThesisen_US
dc.contributor.departmentEnvironmental Engineeringen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineEnvironmental Planningen_US
dc.contributor.committeechairPruden-Bagchi, Amy Jillen_US
dc.contributor.committeememberNovak, John T.en_US
dc.contributor.committeememberVikesland, Peter J.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08152011-132615/en_US
dc.date.sdate2011-08-15en_US
dc.date.rdate2011-09-08
dc.date.adate2011-09-08en_US


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