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dc.contributor.authorAljabri, Hareb Mohammeden_US
dc.date.accessioned2014-03-14T20:44:17Z
dc.date.available2014-03-14T20:44:17Z
dc.date.issued2010-08-16en_US
dc.identifier.otheretd-08262010-121034en_US
dc.identifier.urihttp://hdl.handle.net/10919/34788
dc.description.abstractA major pathway of tyramine and dopamine synthesis in insects is through the decarboxylation of tyrosine and DOPA, respectively. Although tyrosine decarboxylase (TDC) has been mentioned in some reports, it has never been critically analyzed. The high sequence identity shared by tyrosine decarboxylase and DOPA decarboxylase in insects, and the similar structures of the substrates, tyrosine and DOPA, raise the possibility that both tyrosine decarboxylase and DOPA decarboxylase (DDC) have activities to tyrosine and DOPA. In this study, after tyrosine decarboxylase and DOPA decarboxylase enzymes of Anopheles gambiae were expressed, their substrate specificities and biochemical properties were critically analyzed. My results provide clear biochemical evidence establishing that the mosquito tyrosine decarboxylase functions primarily on the production of tyramine with low activity to DOPA. In contrast, mosquito DOPA decarboxylase is highly specific to DOPA with essentially no activity to tyrosine.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartAljabri_HM_T_2010.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectTyrosineen_US
dc.subjectDOPA decarboxylase (DDC)en_US
dc.subjectTyrosine decarboxylase (TDC)en_US
dc.subjectTyramineen_US
dc.subjectDopamineen_US
dc.titleComparative analysis of Anopheles gambiae L-tyrosine decarboxylase and L-DOPA decarboxylaseen_US
dc.typeThesisen_US
dc.contributor.departmentBiochemistryen_US
dc.description.degreeMaster of Science in Life Sciencesen_US
thesis.degree.nameMaster of Science in Life Sciencesen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineBiochemistryen_US
dc.contributor.committeechairLi, Jianyongen_US
dc.contributor.committeememberLarson, Timothy J.en_US
dc.contributor.committeememberTu, Zhijian Jakeen_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08262010-121034/en_US
dc.date.sdate2010-08-26en_US
dc.date.rdate2012-04-24
dc.date.adate2010-09-14en_US


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