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dc.contributor.authorRittler, Matthew Roberten_US

Duchenne's muscular dystrophy (DMD) is a debilitating disease that affects approximately 1 in 3500 boys, with many DMD patients dying before the age of 20 due to cardio-respiratory complications. DMD is the result of defects in the gene that encodes dystrophin, an integral muscle membrane protein. Although the genetic defect has been identified, the relation between the absence of expressed dystrophin and the mechanisms leading to its onset are still unclear. One possibility is that disrupted calcium (Ca2+) handling by the sarcoplasmic reticulum (SR) leads to an increased cytosolic Ca2+ concentration that activates proteolytic and apoptotic pathways that initiate muscle fiber death. However, little is known about the role of disrupted SR function in the onset of DMD.

The purpose of this study was to test the hypothesis that altered calcium cycling by the SR could contribute to elevated cytosolic Ca2+ levels in the early stages of DMD, and thereby account for the onset of disease pathogenesis. Rates of SR Ca2+ uptake and release were determined in quadriceps muscles obtained from maturing dystrophic and control mice prior to the overt signs of the disease at ages ~9 and 21 days. In addition, the content of several key Ca2+ handling proteins, including two isoforms of the sarco(endo)plasmic reticulum ATPase pump (SERCA 1 & 2), ryanodine receptor type 1 (RyR1), parvalbumin, and calsequestrin were determined by Western analysis. Two dystrophic mouse models were used, the mdx mouse which lacks dystrophin, and the mdx:utrophin-deficient (mdx:utrn-/-) mouse which also lacks utrophin, a protein homolog of dystrophin.

The rate of SR Ca2+ uptake in quadriceps muscles of mdx/utrn-/- mice aged 21 days was 73.1% and 61.3% higher than age-matched control and mdx muscles, respectively (p < 0.05). There was no difference in SR Ca2+ release rates between the genotypes at either age. There were significant increases in the content of each of the calcium handling proteins with age (p < 0.05), but no significant differences were detected between genotypes at either age. These data demonstrate the Ca2+ release rates of dystrophic SR are not compromised, but suggest the increased uptake rates of mdx:utrn-/- SR may be an adaptation to increased cytosolic calcium levels, and/or be due to changes in intrinsic SERCA function and/or regulation. The role of increased SR Ca2+ uptakes rates in onset of DMD pathogenesis can not be directly determined from the present data; therefore it is suggested that future studies directly assess cytosolic Ca2+ concentration and examine the role of SERCA regulatory proteins in intact fibers obtained from mdx:utrn-/- muscles at age 21 days.

dc.publisherVirginia Techen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.titleSarcoplasmic Reticulum Calcium Handling in Maturing Skeletal Muscle From Two Models of Dystrophic Miceen_US
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen_US of Scienceen_US Polytechnic Institute and State Universityen_US
dc.contributor.committeechairGrange, Robert W.en_US
dc.contributor.committeememberWilliams, Jay H.en_US
dc.contributor.committeememberDuncan, Robert B. Jr.en_US

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