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dc.contributor.authorBorris, Douglas J.en_US
dc.date.accessioned2014-03-14T20:48:13Z
dc.date.available2014-03-14T20:48:13Z
dc.date.issued2002-11-08en_US
dc.identifier.otheretd-11222002-024854en_US
dc.identifier.urihttp://hdl.handle.net/10919/35791
dc.description.abstractBacteriophage Felix O1, also known as enterobacteria phage O1, has been used to type Salmonella Typhi and is an excellent candidate for use in bioremedial and therapeutic applications. It has extremely high intra-species specificity and is strictly virulent in nature, unable to undergo lysogeny. To facilitate the development of the bacteriophage for use in these areas, the full sequence of the genome had been elucidated previously. In this work, identification and classification of functional coding sequences via reverse transcriptase-polymerase chain reaction was performed.

All of the 115 putative open reading frames (ORFs) studied were found to be functional. 53.0%, 9.6%, and 18.3% of the ORFs investigated were found to initiate expression early, middle or late in the lytic cycle, respectively. Expression of the remaining 19.1% ORFs was evident when the amount of total RNA was increased and when samples were taken at a later time point. Comparisons between bacteriophage Felix O1 and the phage with the most shared homologs, phage T4, revealed many similarities in times of gene expression.

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dc.publisherVirginia Techen_US
dc.relation.haspartBorris.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectBacteriophageen_US
dc.subjectSalmonellaen_US
dc.subjectFelix O1en_US
dc.titleTemporal Analysis of Bacteriophage Felix O1 Gene Expressionen_US
dc.typeThesisen_US
dc.contributor.departmentVeterinary Medical Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineVeterinary Medical Sciencesen_US
dc.contributor.committeememberEyestone, Willard H.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11222002-024854/en_US
dc.contributor.committeecochairPierson, Frank Williamen_US
dc.contributor.committeecochairSriranganathan, Nammalwaren_US
dc.date.sdate2002-11-22en_US
dc.date.rdate2004-03-17
dc.date.adate2003-03-17en_US


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