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dc.contributor.authorLowe, Jeanetteen_US
dc.date.accessioned2014-03-14T20:48:44Z
dc.date.available2014-03-14T20:48:44Z
dc.date.issued1999-11-19en_US
dc.identifier.otheretd-120199-164438en_US
dc.identifier.urihttp://hdl.handle.net/10919/35902
dc.description.abstractThe objective of this study was to identify peptide ligands, using phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for biopanning. Peptides derived from the amplified pools were sequenced and studied. The phage display for in vivo ovary experiments yielded no pool of peptides after two cycles of biopanning and re-amplification. With the same initial concentration of a random 7-mer or 12-mer library, there were repeating sequences derived after three and four biopanning cycles on mouse embryos and unfertilized ova. The sequences were not distinguishable from a control group. Subsequent experimentation using a random 15-mer library to select for internalized phage-peptides yielded two apparent consensus sequences, RNVPPIFNDVYWIAF (9/32 or 28%) and HGRFILPWWYAFSPS (11/32 or 34%). The 15-mer control group yielded no clones. The deduced peptide sequences were compared to known sequences to ascertain their uniqueness. No significant similarities were found, yielding two possible novel motifs. Through this adapted process of phage display and further research, the phage display technology may be used as a tool in the recognition of specific mouse gamete sites. By identifying binding sites of mouse gametes, the peptides might be exploited as a means of studying the embryo cell surface or cytoplasmic components and mouse sperm-egg interactions. Such peptides may also be used for macromolecule delivery in transfection or transgenesis.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartJLthesisETD.pdfen_US
dc.rightsI hereby grant to Virginia Tech or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.en_US
dc.subjectphage displayen_US
dc.subjectzona pellucidaen_US
dc.subjectmouse embryologyen_US
dc.subjecttransgenesisen_US
dc.titlePhage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucidaen_US
dc.typeThesisen_US
dc.contributor.departmentDairy Scienceen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineDairy Scienceen_US
dc.contributor.committeechairGwazdauskas, Francis C.en_US
dc.contributor.committeememberWong, Eric A.en_US
dc.contributor.committeememberRussell, Christopher G.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-120199-164438/en_US
dc.date.sdate1999-12-01en_US
dc.date.rdate2000-07-11
dc.date.adate1999-07-11en_US


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