Show simple item record

dc.contributor.authorElhadj, Selimen_US
dc.date.accessioned2014-03-14T20:57:49Z
dc.date.available2014-03-14T20:57:49Z
dc.date.issued1998-05-05en_US
dc.identifier.otheretd-41298-161018en_US
dc.identifier.urihttp://hdl.handle.net/10919/37167
dc.description.abstractThe recovery of pharmaceuticals from blood plasma is often expensive, limited, and is subject to contamination. The objective of this feasibility study is to present recovery designs and the most significant parameters affecting manufacturing costs of human Serum Albumin (hSA), human Protein C (hPC), and Factor VIII (FVIII) from the milk of transgenic livestock. All the processes presented are based on chromatography columns to achieve separation. The sensitivity of the manufacturing cost of the protein to various parameters are illustrated graphically and discussed, along with the cost distribution percentages for each type of process. It was found that manufacturing costs decrease with superficial velocity, expression levels, column yields, column capacities, and scale of production increases with all other parameters remaining constant. However the estimated cost decreases tend to level out at higher values of the parameters. Finally a judicious choice of separation scheme, such as Fall-Through (FT) versus Direct Adsorption (DA) for hSA production, can decrease the protein cost by as much as 38%, due to savings from wash and elution buffers. The costs associated with the production of hSA (DA process), hSA (FT process), hPC, and FVIII are 3.56, 2.20, 129, and 11,780 $/gr respectively. By means of the optimization of manufacturing parameters to reduce costs, key areas of emphasis for process development were identified.en_US
dc.publisherVirginia Techen_US
dc.relation.haspartMajpaper.pdfen_US
dc.rightsI hereby grant to Virginia Tech or its agents the right to archive and to make available my thesis or dissertation in whole or in part in the University Libraries in all forms of media, now or hereafter known. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.en_US
dc.subjecthuman serum albuminen_US
dc.subjecthuman protein Cen_US
dc.subjectfactor VIIIen_US
dc.subjecttherapeutic proteinen_US
dc.subjectmanufacturing costen_US
dc.subjectrecoveryen_US
dc.subjectmilken_US
dc.subjecttransgenicen_US
dc.titleEconomic Feasibility of the Recovery of Therapeutic Proteins From the Milk of Transgenic Livestocken_US
dc.typeMajor paperen_US
dc.contributor.departmentChemical Engineeringen_US
dc.description.degreeMaster of Engineeringen_US
thesis.degree.nameMaster of Engineeringen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineChemical Engineeringen_US
dc.contributor.committeechairVelander, William H.en_US
dc.contributor.committeememberWilliams, Kimberly Forstenen_US
dc.contributor.committeememberDavis, Richey M.en_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-41298-161018/en_US
dc.date.sdate1998-05-05en_US
dc.date.rdate1999-05-12
dc.date.adate1998-05-12en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record