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dc.contributor.authorRuark, Edwin Warrenen
dc.date.accessioned2014-03-14T21:08:49Zen
dc.date.available2014-03-14T21:08:49Zen
dc.date.issued1970en
dc.identifier.otheretd-01132010-020032en
dc.identifier.urihttp://hdl.handle.net/10919/37198en
dc.description.abstractThe effect of triiodothyronine (L-T₃) on the protein, RNA and DNA fractions of the thymus nuclei of growth-arrested sulfaguanidine-fed rats has been studied. A single dose of 15 µg of L-T₃ stimulated the incorporation of ¹⁴C-labelled amino acids into total nuclear protein, whole histone and histone fractions. The incorporation of the labelled amino acid into nuclear protein was increased 40 percent over the control 4 hours after the administration of L-T₃. The incorporation of alanine-1-¹⁴C into the total histone fraction reached a maximum 4 hours after the administration of L-T₃ and then decreased 8 and 16 hours after the administration of L-T₃. The incorporation of alanine-1-¹⁴C into histone fraction f₁ reached a maximum at 4 hours after the administration of L-T₃ while the incorporation into fractions f2a, f2b and f₃ did not reach a maximum until 8 hours after the administration of L-T₃. Time course studies showed that the protein and RNA moieties of thymus chromatin of rats injected with a single dose of L-T₃ was increased to a maximum at 4 hours. The Tm of chromatin was increased 2° by L-T₃ administration as early as 2 hours. However, the Tm of DNA was not affected. Four hours after the administration of L-T₃ the template efficiency increased 171 percent. The template efficiency of DNA was not affected by L-T₃. These results demonstrate that thyroid hormones modify the chromatin in such a way that the template efficiency is increased. An hypothesis for the mechanism of thyroid hormone action was presented.en
dc.format.extent89 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.isformatofOCLC# 22366655en
dc.relation.haspartLD5655.V856_1970.R8.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1970.R8en
dc.subject.lcshRatsen
dc.subject.lcshThymusen
dc.subject.lcshThyroid hormonesen
dc.titleThe response of rat thymus nuclei to thyroid hormonesen
dc.typeDissertationen
dc.contributor.departmentBiochemistry and Nutritionen
dc.description.degreePh. D.en
thesis.degree.namePh. D.en
thesis.degree.leveldoctoralen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.disciplineBiochemistry and Nutritionen
dc.type.dcmitypeTexten
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-01132010-020032/en
dc.date.sdate2010-01-13en
dc.date.rdate2010-01-13en
dc.date.adate2010-01-13en


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